Purple sweet potato leaf extract-containing composition capable of preventing hair loss and strengthening hair and preparation method of purple sweet potato leaf extract-containing composition

Abstract

The invention provides a purple sweet potato leaf extract-containing composition with the effects of preventing hair loss and strengthening hair and a preparation method thereof.The composition is prepared from, by weight, 100-120 parts of purple sweet potato leaves, 25-35 parts of hyacinth bean coats, 25-35 parts of pyrus bretschneideri peels, 25-35 parts of Chinese waxgourd peels, 8-12 parts of Wuzmann grass, 90-110 parts of sophora flowers, 25-35 parts of morinda citrifolia ferment, 25-35 parts of cactus powder and 8-12 parts of snake slough. 25-35 parts of Chinese arborvitae twig and leaf and 25-35 parts of mulberry leaf. According to the composition containing the purple sweet potato leaf extract, all the extracts are compounded, the formula is scientific and reasonable, synergistic effect is achieved, irritation is small, safety is high, in the using process, hair roots are nourished, effective components efficiently penetrate into hair follicles, the activity of cells around the hair follicles is activated, hair growth is effectively promoted, alopecia is prevented, and the effect is good. The effects of preventing hair loss and fixing hair are achieved. Meanwhile, the composition has an efficient inhibition effect on 5alpha-reductase and main microorganisms, and the probability of seborrheic alopecia is reduced. In addition, the shampoo also has multiple effects of soothing the scalp, improving the scalp environment and nourishing the hair.

Description

Technical Field

[0001] The invention relates to the technical field of plant extracts, and in particular to a composition containing a purple sweet potato leaf extract and having the functions of preventing hair loss and strengthening hair, and a preparation method thereof. Background Art

[0002] Seborrheic alopecia, also known as androgenic alopecia (AGA), belongs to the category of "oily wind" and "hair-moth hair loss" in traditional Chinese medicine. It is mainly manifested by the gradual receding of the hairline on the forehead, or the gradual thinning and loss of hair on the top of the head, accompanied by increased dandruff, oily scalp, and obvious itching. The main manifestations for men are the M-shaped upward expansion of the temples on both sides of the forehead, forming a horseshoe-shaped appearance; the main manifestations for women are the decrease in hair density on the top of the head, sparse hair, and the expansion of the hair loss area on the top, forming a "Christmas tree"-like appearance. This disease is a common, frequently occurring, and difficult-to-treat disease in clinical dermatology. A large number of studies have shown that 80% of male and 50% of female patients can attribute their hair loss to AGA, which not only affects the quality of life of patients, but also their psychological state.

[0003] Finasteride is a type II 5α-reductase inhibitor that can block the conversion of testosterone (T) into the more active dihydrotestosterone (DHT), thereby weakening the inhibitory effect of androgens on hair follicles. The therapeutic mechanism of minoxidil is not yet fully understood, and may involve multiple pathways, such as opening the ATP-dependent K + Channels, affecting AR activity and stability, etc. Although both are widely used in clinical practice, neither can completely block the progression of the disease, and both have adverse reactions to varying degrees. Therefore, the use of natural herbal plants to treat hair loss has become a research hotspot.

[0004] However, the existing shampoos with pure Chinese medicine formulas have the problem of poor effect or slow effect, while pure chemical shampoos are more irritating to the scalp and even damage the scalp tissue, which has safety issues. In view of the problems of poor anti-hair loss and hair fixation effect and strong irritation in the existing hair lotions, it is very necessary to find a hair lotion with less irritation and excellent anti-hair loss and hair fixation effect. Summary of the invention

[0005] In view of this, the present invention provides a composition containing a purple sweet potato leaf extract having the effects of preventing hair loss and strengthening hair and a preparation method thereof.

[0006] The technical solution of the present invention is achieved in this way: A composition containing a purple sweet potato leaf extract with the effect of preventing hair loss and strengthening hair, comprising the following raw materials in parts by weight: 100-120 parts of purple sweet potato leaves, 25-35 parts of lentil skins, 25-35 parts of pear peels, 25-35 parts of wax gourd peels and 8-12 parts of osmanthus sinensis, 100-120 parts of purple sweet potato leaves, 25-35 parts of lentil skins, 25-35 parts of pear peels, 25-35 parts of wax gourd peels and 8-12 parts of osmanthus sinensis, 90-110 parts of Sophora japonica flowers, 25-35 parts of noni fruit enzymes, 25-35 parts of cactus powder, 8-12 parts of snake skins, 25-35 parts of Platycladus orientalis leaves and 25-35 parts of mulberry leaves.

[0007] A method for preparing a composition containing a purple sweet potato leaf extract having the effect of preventing hair loss and strengthening hair, the specific steps comprising: (1) Crush dried purple sweet potato leaves, lentil skins and Usman grass and place them in supercritical CO 2 In the extraction device, firstly extract at 40-50°C and 10-20MPa for 20-40min, then continue to extract at 55-65°C and 25-40MPa for 20-40min, add the extract into a separation kettle for separation, and concentrate under reduced pressure to obtain extract a; (2) crushing fresh pear peel and wax gourd peel, adding them into deionized water, and soaking them under high pressure. After that, ultrasonic extraction is performed, and the extract is concentrated under reduced pressure to obtain extract b; (3) Adding Platycladus orientalis leaves and mulberry leaves to an ethanol solution, soaking and extracting at a temperature of 4-10°C, collecting the extract, standing at low temperature, centrifuging to obtain a supernatant, and concentrating under reduced pressure to obtain extract c; (4) adding Sophora japonica flower and cactus powder to water, performing hot-pressing distillation extraction, and concentrating under reduced pressure to obtain extract d; (5) Add noni fruit enzyme and snake slough into water and soak for 30-60 minutes, then boil them, first boil them over high heat for 30-50 minutes, then boil them over low heat for 20-30 minutes, keep them slightly boiling, filter them, filter the filtrate on a microporous filter membrane, and concentrate the filtrate under reduced pressure to obtain extract e; (6) The extract a, the extract b, the extract c, the extract d and the extract e are mixed evenly to obtain a composition containing a purple sweet potato leaf extract.

[0008] Furthermore, in step (2), the liquid ratio of the crushed pear peel and wax gourd peel to deionized water is 1:15-20 g / mL; the high-pressure immersion is carried out at 20-25°C and 6-10 MPa for 20-30 min; the ultrasonic extraction is performed 2-4 times, each time for 20-30 min, at a temperature of 60-80°C and an ultrasonic frequency of 20-30 kHz.

[0009] Furthermore, in step (3), the solid-liquid ratio of the Platycladus orientalis leaves and mulberry leaves to the ethanol solution is 1:9-11 g / mL; the concentration of the ethanol solution is 70%-90% v / v; the number of immersion extractions is 2-3 times, each extraction is 4-6 hours; and the low-temperature standing is standing at 0-4°C for 6-10 hours.

[0010] Furthermore, in step (4), the material-liquid ratio of the Sophora japonica flower and cactus powder to water is 1:6-10 g / mL; the hot pressure distillation is carried out at 0.2-0.3 MPa and 80-100° C. for 2-4 hours; In step (5), the liquid ratio of the noni fruit enzyme, snake skin and water is 1:10-12 g / mL.

[0011] The invention discloses an application of a composition containing a purple sweet potato leaf extract and having the effects of preventing hair loss and strengthening hair in the preparation of a hair lotion product.

[0012] Furthermore, the preparation method of the hair lotion product comprises: (1) adding a composition containing purple sweet potato leaf extract into water, stirring and heating the water to above 70° C. to form a liquid medicine; (2) Amino acid thickener PEG-120, sodium lauroyl glutamate, cocamidopropyl betaine, NaCl, potassium sorbate, tea polyphenols, guar gum and aqueous azone are added to the liquid in sequence, stirred evenly, and water is added to 1000 mL to obtain the target hair wash product.

[0013] Furthermore, in step (1), the solid-liquid ratio of the composition containing purple sweet potato leaf extract to water is 1:5-7 g / mL.

[0014] Furthermore, in step (2), the following raw materials are included in parts by weight: 25-35 parts of amino acid thickener PEG-120, 90-110 parts of sodium lauroyl glutamate, cocamidopropyl betaine, 18-22 parts of NaCl, 4-6 parts of potassium sorbate, 4-6 parts of tea polyphenols, 1-3 parts of guar gum and 9-11 parts of aqueous azone.

[0015] Compared with the prior art, the present invention has the following beneficial effects: 1. The present invention provides a composition containing purple sweet potato leaf extract with anti-hair loss and hair fixation, which is compounded by extracts such as purple sweet potato leaf, lentil coat, pear peel, wax gourd peel and Usman grass, and the formula is scientific and reasonable, synergistic, less irritating, and highly safe. During use, the hair root can be nourished, so that the effective ingredients can efficiently penetrate into healthy hair follicles, activate the cell activity around the hair follicles, thereby effectively promoting the growth of hair, effectively preventing hair loss, and playing an effective anti-hair loss and hair fixation effect. At the same time, the 5α-reductase and main microorganisms such as Malassezia furfur, Propionibacterium acnes and Staphylococcus aureus that cause seborrheic alopecia are efficiently inhibited, thereby reducing the probability of seborrheic alopecia. In addition, it also has the effects of soothing the scalp, improving the scalp environment, nourishing the hair, enhancing the elasticity and gloss of the hair, reducing hair breakage and shedding, and having multiple effects.

[0016] 2. The various extracts obtained by the specific extraction method of the present invention are used to prepare hair lotions with short treatment courses, quick results and long-term anti-hair loss and hair strengthening effects. Generally, a good anti-hair loss and hair strengthening effect can be achieved after four to eight weeks of use, and the application prospect is good. DETAILED DESCRIPTION

[0017] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0018] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0019] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0020] Example 1 The composition containing purple sweet potato leaf extract of this embodiment includes the following raw materials in parts by weight: 110 parts of purple sweet potato leaves, 30 parts of lentil skins, 30 parts of pear peels, 30 parts of wax gourd peels and 10 parts of Usman grass, 100 parts of Sophora japonica flowers, 30 parts of Noni fruit enzymes, 30 parts of cactus powder, 10 parts of snake skins, 30 parts of Platycladus orientalis leaves and 30 parts of mulberry leaves.

[0021] The preparation method of the above-mentioned composition containing purple sweet potato leaf extract comprises the following specific steps: (1) Crush dried purple sweet potato leaves, lentil skins and Usman grass and place them in supercritical CO 2 In the extraction device, the extraction is first performed at 45°C and 15MPa for 340min, and then at 60°C and 30MPa for 30min. The extract is added to a separation kettle for separation and concentrated under reduced pressure to obtain extract a. (2) Fresh pear peel and wax gourd peel were crushed, added into deionized water at a solid-liquid ratio of 1:18 g / mL, and soaked at 23°C and 8 MPa for 25 min. After completion, ultrasonic extraction was performed at 70°C and 25 kHz for 3 times, each time for 25 min, and the extract was concentrated under reduced pressure to obtain extract b; (3) According to the solid-liquid ratio of 1:10 g / mL, Platycladus orientalis leaves and mulberry leaves were added to 80% v / v ethanol solution, immersed in 6°C for 3 times for extraction, each extraction for 4 h, and the extract was collected and allowed to stand at 2°C for 8 h. The supernatant was obtained by centrifugation and concentrated under reduced pressure to obtain extract c; (4) Sophora japonica flower and cactus powder were added to water at a solid-liquid ratio of 1:8 g / mL, and hot-pressed distillation was performed, specifically: treatment at 0.25 MPa and 90 °C for 3 h, and reduced pressure concentration was performed to obtain extract d; (5) Noni fruit enzyme and snake skin were added to water at a solid-liquid ratio of 1:11 g / mL, soaked for 45 min, and then decocted. First, boiled over high heat for 40 min, then decocted over low heat for 25 min, kept slightly boiling, filtered, and the filtrate was finely filtered on a microporous filter membrane. The fine filtrate was concentrated under reduced pressure to obtain extract e; (6) The extract a, the extract b, the extract c, the extract d and the extract e are mixed evenly to obtain a composition containing a purple sweet potato leaf extract.

[0022] Example 2 The composition containing purple sweet potato leaf extract of this embodiment includes the following raw materials in parts by weight: 100 parts of purple sweet potato leaves, 25 parts of lentil skins, 25 parts of pear peels, 25 parts of wax gourd peels and 8 parts of Usman grass, 90 parts of Sophora japonica flowers, 25 parts of Noni fruit enzymes, 25 parts of cactus powder, 8 parts of snake skins, 25 parts of Platycladus orientalis leaves and 25 parts of mulberry leaves.

[0023] The preparation method of the above-mentioned composition containing purple sweet potato leaf extract comprises the following specific steps: (1) Crush dried purple sweet potato leaves, lentil skins and Usman grass and place them in supercritical CO 2 In the extraction device, the extraction is first performed at 40°C and 10MPa for 20 minutes, and then at 55°C and 25MPa for 20 minutes. The extract is added to a separation kettle for separation, and concentrated under reduced pressure to obtain extract a. (2) Fresh pear peel and wax gourd peel were crushed, added into deionized water at a solid-liquid ratio of 1:15 g / mL, and soaked at 20°C and 6 MPa for 20 min. After completion, ultrasonic extraction was performed twice at 60°C and 20 kHz, each time for 20 min, and the extract was concentrated under reduced pressure to obtain extract b; (3) According to the solid-liquid ratio of 1:9 g / mL, Platycladus orientalis leaves and mulberry leaves were added to 70% v / v ethanol solution, immersed twice at 4°C for extraction, each extraction for 4 h, and the extract was collected and allowed to stand at 0°C for 6 h. The supernatant was obtained by centrifugation and concentrated under reduced pressure to obtain extract c; (4) Sophora japonica flower and cactus powder were added to water at a solid-liquid ratio of 1:6 g / mL, and hot-pressed distillation was performed, specifically: treatment at 0.2 MPa and 80 °C for 2 h, and concentration under reduced pressure to obtain extract d; (5) Noni fruit enzyme and snake skin were added to water at a solid-liquid ratio of 1:10 g / mL, soaked for 30 min, and then decocted. First, boiled over high heat for 30 min, then decocted over low heat for 20 min, kept slightly boiling, filtered, and the filtrate was finely filtered on a microporous filter membrane. The fine filtrate was concentrated under reduced pressure to obtain extract e; (6) The extract a, the extract b, the extract c, the extract d and the extract e are mixed evenly to obtain a composition containing a purple sweet potato leaf extract.

[0024] Example 3 The composition containing purple sweet potato leaf extract of this embodiment includes the following raw materials in parts by weight: 120 parts of purple sweet potato leaves, 35 parts of lentil skins, 35 parts of pear peels, 35 parts of wax gourd peels and 12 parts of Usman grass, 110 parts of Sophora japonica flowers, 35 parts of Noni fruit enzymes, 35 parts of cactus powder, 12 parts of snake skins, 35 parts of Platycladus orientalis leaves and 35 parts of mulberry leaves.

[0025] The preparation method of the above-mentioned composition containing purple sweet potato leaf extract comprises the following specific steps: (1) Crush dried purple sweet potato leaves, lentil skins and Usman grass and place them in supercritical CO 2 In the extraction device, the extraction is first performed at 50°C and 20 MPa for 40 min, and then at 65°C and 40 MPa for another 40 min. The extract is added to a separation kettle for separation, and concentrated under reduced pressure to obtain extract a. (2) Fresh pear peel and wax gourd peel were crushed, added into deionized water at a solid-liquid ratio of 1:20 g / mL, and soaked at 25°C and 10 MPa for 30 min. After completion, ultrasonic extraction was performed at 80°C and 30 kHz for 4 times, each time for 30 min, and the extract was concentrated under reduced pressure to obtain extract b; (3) According to the solid-liquid ratio of 1:11 g / mL, Platycladus orientalis leaves and mulberry leaves were added to 90% v / v ethanol solution, immersed in water for 3 times at 10°C for extraction, each extraction for 6 h, and the extract was collected and allowed to stand at 4°C for 10 h. The supernatant was obtained by centrifugation and concentrated under reduced pressure to obtain extract c; (4) Sophora japonica flower and cactus powder were added to water at a solid-liquid ratio of 1:10 g / mL, and hot-pressed distillation was performed, specifically: treatment at 0.3 MPa and 100 °C for 4 h, and reduced pressure concentration was performed to obtain extract d; (5) Noni fruit enzyme and snake skin were added to water at a solid-liquid ratio of 1:12 g / mL, and the mixture was soaked for 60 min, then decocted, first boiled over high heat for 50 min, then decocted over low heat for 30 min, kept at a slight boil, filtered, and the filtrate was finely filtered on a microporous filter membrane, and the fine filtrate was concentrated under reduced pressure to obtain extract e; (6) The extract a, the extract b, the extract c, the extract d and the extract e are mixed evenly to obtain a composition containing a purple sweet potato leaf extract.

[0026] Comparative Example 1 The difference from Example 1 is that the amount of raw materials is different, and the rest is consistent with Example 1.

[0027] That is, the composition containing purple sweet potato leaf extract in this comparative example includes the following raw materials in parts by weight: 90 parts of purple sweet potato leaves, 20 parts of lentil skins, 20 parts of duck pear peels, 20 parts of wax gourd peels and 5 parts of Usman grass, 120 parts of Sophora japonica flowers, 40 parts of Noni fruit enzymes, 40 parts of cactus powder, 15 parts of snake skins, 40 parts of Platycladus orientalis leaves and 40 parts of mulberry leaves.

[0028] Comparative Example 2 The difference from Example 1 is that: lentil skins are not included, the amount of the missing substance is distributed to the corresponding components according to the proportion of other substances in Example 1, and the rest is consistent with Example 1.

[0029] Comparative Example 3 The difference from Example 1 is that: pear peel and wax gourd peel are not contained, and the amount of the missing substances is distributed to the corresponding components according to the proportion of other substances in Example 1. The rest is consistent with Example 1.

[0030] Comparative Example 4 The difference from Example 1 is that the preparation method is different, and the rest is consistent with Example 1.

[0031] The composition containing purple sweet potato leaf extract of this comparative example includes the following raw materials in parts by weight: 110 parts of purple sweet potato leaves, 30 parts of lentil skins, 30 parts of pear peels, 30 parts of wax gourd peels and 10 parts of Usman grass, 100 parts of Sophora japonica flowers, 30 parts of Noni fruit enzymes, 30 parts of cactus powder, 10 parts of snake skins, 30 parts of Platycladus orientalis leaves and 30 parts of mulberry leaves.

[0032] The preparation method of the above-mentioned composition containing purple sweet potato leaf extract comprises the following specific steps: According to the solid-liquid ratio of 1:8g / mL, purple sweet potato leaves, lentil skins, pear peels, wax gourd peels, Usman grass, Sophora japonica, Noni fruit enzyme, cactus powder, snake skins, Platycladus orientalis leaves and mulberry leaves are added to water, boiled at 70°C for 12h to obtain a medicinal solution, filtered, and concentrated under reduced pressure to obtain a composition containing purple sweet potato leaf extract.

[0033] Test Example 1-5α-Reductase Activity Effect Experimental principle: The main cause of seborrheic alopecia is that 5ɑ-reductase in the scalp hair follicles can convert testosterone into highly active dihydrotestosterone (DHT), which has an affinity with androgen receptor (AR) more than 5 times greater than testosterone. DHT binds to AR concentrated in the hair follicle unit and enters the cell nucleus, thereby producing a series of biological effects, reducing the concentration of intracellular cyclic adenosine monophosphate, shortening the hair growth cycle, and miniaturizing the hair follicles, thereby causing hair loss. Therefore, the oil control and anti-hair loss effect can be evaluated by detecting the inhibition rate of the test substance on 5ɑ-reductase.

[0034] Experimental method: (1) Dilute the samples of Examples 1-3 and Comparative Examples 1-4 N times with 0.1 mol / L Tris-HCl buffer for the next test. Take out the required strips from the aluminum bag after equilibration at room temperature for 20 minutes. Seal the remaining strips with a ziplock bag and place them back at 4°C. Note: The concentration of this sample should not be too high, as it will lead to an imbalance between the sample and the reductase concentration. A preliminary experiment is required to find the most suitable concentration.

[0035] (2) Set up blank wells, positive control wells, sample wells and negative control wells; except for the blank group, no enzyme was added. In addition, 50 μL of 5α-reductase was added to each well of the other groups and incubated in a constant temperature incubator at 37°C for 10 min; add the samples of Examples 1-3 and Comparative Examples 1-4 to the sample wells, and add the positive control (EGGG with a certain molar concentration) to the positive control wells; note that the molar concentration ratio between the sample and the positive control should be appropriate (at least less than 10 times); do not add any sample or inhibitor to the negative control; incubate the microplate after sample addition for 10 min, and wash the plate 5 times (the washing solution is provided by the kit, and the original washing solution is diluted 20 times for later use).

[0036] (3) Add 100uL of horseradish peroxidase (HRP)-labeled detection antibody to each well of the negative control group, positive control group, and sample wells, seal the reaction wells with a sealing film, and incubate in a 37°C water bath or incubator for 60 min.

[0037] (4) Discard the liquid, pat dry on absorbent paper, and wash the plate 5 times.

[0038] (5) Add 50 μL of substrate A and B to each well and incubate at 37°C in the dark for 15 min.

[0039] (6) Add 50 μL of stop solution to each well and measure the OD value of each well at a wavelength of 450 nm within 15 min.

[0040] Among them, the inhibition rate of 5α-reductase is calculated as: ① Sample inhibition rate: inhibition rate (%) = 100 × (OD 空白 +OD 阴性对照 -OD 样品） / (OD 空白 +OD 阴性对照） ② Positive control inhibition rate: inhibition rate (%) = 100 × (OD 空白 +OD 阴性对照 -OD 阳性对照） / (OD 空白 +OD 阴性对照） The results are shown in Table 1.

[0041] Table 1

[0042] As can be seen from Table 1, the compositions of Examples 1-3 of the present invention have a higher inhibition rate on 5α-reductase, while the compositions of Comparative Examples 1-4 have a lower inhibition rate on 5α-reductase, indicating that the synergistic synergistic effect of the compositions of the present invention can better exert the effect of inhibiting the activity of 5α-reductase and has a better anti-hair loss effect.

[0043] Test Example 2-Bacterial Inhibition Test Experimental principle: The main microorganisms that cause seborrheic alopecia include aerobic fungi (Malassezia furfur), anaerobic bacteria (Propionibacterium acnes) and facultative aerobic bacteria (Staphylococcus aureus). Therefore, the inhibition zone test is used to determine the inhibitory effect of the test sample on Malassezia furfur (Propionibacterium acnes ATCC 44344), Propionibacterium acnes (Propionibacterium acnes ATCC 11827) and Staphylococcus aureus (Staphylococcus aureusATCC49775) to evaluate the antibacterial and anti-hair loss effect.

[0044] Experimental method: The compositions prepared in Examples 1-3 and Comparative Examples 1-4 were diluted with dimethyl sulfoxide (DMSO) to a sample with a mass fraction of 5%, and the following test was performed: Aseptic operation was used, different culture methods were used for different microorganisms, fungal culture medium was used for Malassezia furfur, anaerobic culture method was used for Propionibacterium acnes, and LB broth culture medium was used for Staphylococcus aureus. The three activated bacteria were prepared into bacterial suspensions, and 0.2 mL of each bacterial suspension was smeared on the Leeming & Notman medium plate, and the Oxford cup was placed vertically on the surface of the corresponding medium with tweezers, and gently pressed to make it contact with the medium without gaps. Each plate can hold 3 small tubes, and 0.1 mL of various samples to be tested were dripped into each small tube, and marked to prevent overflow. After anaerobic culture in a 37°C incubator for 3 days, the results were observed. During the culture, on the one hand, the test bacteria began to grow, and on the other hand, the sample to be tested spread around with the Oxford cup as the origin, forming a circular area where the colony could not grow, i.e., the "inhibition zone". The larger the inhibition zone, the better the acne removal effect. The antibacterial activity was determined by the diameter of the inhibition zone.

[0045] The criteria for judging the antibacterial effect are as follows: the diameter of the inhibition zone is greater than 20 mm for high efficiency; the diameter of the inhibition zone is 15-20 mm for medium efficiency; the diameter of the inhibition zone is 10-15 mm for low efficiency, and the diameter of the inhibition zone is less than 10 mm for ineffectiveness.

[0046] The experimental results are shown in Table 2.

[0047] Table 2

[0048] As can be seen from Table 2, the compositions of Examples 1-3 of the present invention have strong antibacterial effects on Malassezia furfur, Propionibacterium acnes, and Staphylococcus aureus, while the compositions of Comparative Examples 1-4 have smaller inhibition zones for Malassezia furfur, Propionibacterium acnes, and Staphylococcus aureus than those of Examples 1-3, indicating that the compositions of the present invention have synergistic effects and can better play the role of inhibiting the main microorganisms that cause hair loss.

[0049] Test Example 3 - Mouse Hair Growth Test 80 rats of half male and half female with similar body weight and hair growth were purchased and divided into a modeling group of 70 and a control group of 10. A 3cm×3cm area was selected from the same position on the back of the rat and dyed yellow with 3% picric acid solution as the test area. Except for the control group, the mice in other groups were subcutaneously injected with testosterone propionate (1mg / kg•d dose) behind the neck for 60 consecutive days, once a day, for modeling, and then depilated to mark the test area. The modeled mice were randomly divided into 7 groups, 10 in each group, respectively, Examples 1-3 and Comparative Examples 1-4, and the compositions prepared by Examples 1-3 and Comparative Examples 1-4 were applied to the test area every day, with the same dosage. The changes in the skin on the back of the mice and the hair growth were observed daily. The control group was not treated after depilation. After 30 days, each group of experimental mice randomly selected 10 longer hairs in the test area, measured their lengths with a vernier caliper, and collected and weighed the hair in the test area. The test results were statistically analyzed, the results were averaged, and the hair in the test area of ​​the mice was observed to see if it was sparse or messy. The experimental results are shown in Table 3.

[0050] Table 3

[0051] As can be seen from Table 3, the compositions prepared in Examples 1-3 of the present invention can nourish the roots of hair, so that the effective ingredients can efficiently penetrate into healthy hair follicles, activate the cell activity around the hair follicles, and thus better promote the growth of mouse hair. At the same time, they can nourish the hair, making the hair smoother. However, the compositions of Comparative Examples 1-4 are not as effective in promoting the growth of mouse hair as Examples 1-3, indicating that the compositions of the present invention synergistically promote the growth of mouse hair.

[0052] Test Example 4-Clinical hair loss prevention test 80 healthy people aged 18-35 with hair loss were randomly selected, 40 men and 40 women, who were required not to use any hair care products within one month, and were randomly divided into 8 groups, with 10 people in each group. Group 1 was used as the control group, and groups 2-8 corresponded to Examples 1-3 and Comparative Examples 1-4 respectively; one day before the experiment, the number of hairs dropped during the washing process in each group was collected, which was recorded as the initial hair loss amount; the people in groups 2-8 used the anti-hair loss and hair-fixing compositions prepared by Examples 1-3 and Comparative Examples 1-4 to wash their hair every 2 days, and after washing their hair for 60 days, the number of hairs dropped during the last washing process was recorded, which was recorded as the final hair loss amount, and then the hair loss inhibition rate was calculated. The control group did not use the anti-hair loss and hair-fixing compositions of the present invention, and washed their hair normally before the experiment.

[0053] Hair loss inhibition rate (100%) = (average initial hair loss amount - average final hair loss amount) / average initial hair loss amount × 100% The results are shown in Table 4.

[0054] Table 4

[0055] As can be seen from Table 4, the compositions prepared in Examples 1-3 of the present invention can effectively reduce the occurrence of hair loss and inhibit hair loss, while the hair loss inhibition rate of the compositions of Comparative Examples 1-4 decreases, indicating that the synergistic effect of the compositions of the present invention can inhibit the occurrence of hair loss.

[0056] Test Example 5-Irritation Test Thirty healthy rabbits weighing (2.0±0.2) kg were randomly divided into three groups, with 10 animals in each group. The hair on both sides of the rabbit back skin was removed 24 hours before the experiment and the skin was checked for injury before the experiment. Injured skin cannot be used for skin irritation test. The composition of Examples 1-3 was prepared into a diluted aqueous solution at a ratio of 1%, and the test sample was applied 3 times a day for 7 consecutive days. The unapplied area can be used as a control for observation. "+" indicates that the rabbit skin is congested and swollen; "++" indicates that the congestion and swelling are still there, but there is an increasing trend; "-" indicates no congestion and swelling.

[0057] The test results are shown in Table 5.

[0058] Table 5

[0059] It can be seen from Table 5 that after the compositions of Examples 1-3 of the present invention were applied to the rabbit skin, there was no congestion or redness or swelling, which indicates that the compositions prepared by the present invention have no irritation to the skin and have excellent safety in use.

[0060] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A composition containing purple sweet potato leaf extract for preventing hair loss and strengthening hair, characterized in that: In terms of weight, the invention comprises the following raw materials: 100-120 parts of purple sweet potato leaves, 25-35 parts of lentil skins, 25-35 parts of pear peels, 25-35 parts of wax gourd peels and 8-12 parts of Usman grass, 100-120 parts of purple sweet potato leaves, 25-35 parts of lentil skins, 25-35 parts of pear peels, 25-35 parts of wax gourd peels and 8-12 parts of Usman grass, 90-110 parts of Sophora japonica flowers, 25-35 parts of Noni fruit enzyme, 25-35 parts of cactus powder, 8-12 parts of snake skins, 25-35 parts of Platycladus orientalis leaves and 25-35 parts of mulberry leaves.

2. The method for preparing a composition containing purple sweet potato leaf extract with anti-hair loss and hair solidification properties as claimed in claim 1, characterized in that: The specific steps include: (1) Crush dried purple sweet potato leaves, lentil skins and Usman grass, place them in a supercritical CO2 extraction device, first extract them at 40-50°C and 10-20MPa for 20-40 min, then continue to extract them at 55-65°C and 25-40MPa for 20-40 min, add the extract into a separation kettle for separation, and concentrate under reduced pressure to obtain extract a; (2) crushing fresh pear peel and wax gourd peel, adding them into deionized water, and soaking them under high pressure. After that, ultrasonic extraction is performed, and the extract is concentrated under reduced pressure to obtain extract b; (3) Adding Platycladus orientalis leaves and mulberry leaves to an ethanol solution, soaking and extracting at a temperature of 4-10°C, collecting the extract, standing at low temperature, centrifuging to obtain a supernatant, and concentrating under reduced pressure to obtain extract c; (4) adding Sophora japonica flower and cactus powder to water, performing hot-pressing distillation extraction, and concentrating under reduced pressure to obtain extract d; (5) Add noni fruit enzyme and snake slough into water and soak for 30-60 minutes, then boil them, first boil them over high heat for 30-50 minutes, then boil them over low heat for 20-30 minutes, keep them slightly boiling, filter them, filter the filtrate on a microporous filter membrane, and concentrate the filtrate under reduced pressure to obtain extract e; (6) The extract a, the extract b, the extract c, the extract d and the extract e are mixed evenly to obtain a composition containing a purple sweet potato leaf extract.

3. The method for preparing a composition containing purple sweet potato leaf extract with anti-hair loss and hair strengthening effects as claimed in claim 2, characterized in that: In step (2), the material-liquid ratio of the crushed pear peel and wax gourd peel to deionized water is 1:15-20 g / mL; the high-pressure immersion is carried out at 20-25°C and 6-10 MPa for 20-30 min; the ultrasonic extraction is performed 2-4 times, each time for 20-30 min, at a temperature of 60-80°C and an ultrasonic frequency of 20-30 kHz.

4. The method for preparing a composition containing purple sweet potato leaf extract with anti-hair loss and hair solidification properties as claimed in claim 2, characterized in that: In step (3), the solid-liquid ratio of the Platycladus orientalis leaves and mulberry leaves to the ethanol solution is 1:9-11 g / mL; the concentration of the ethanol solution is 70%-90% v / v; the number of immersion extractions is 2-3 times, each extraction is 4-6 hours; and the low-temperature standing is standing at 0-4°C for 6-10 hours.

5. The method for preparing a composition containing purple sweet potato leaf extract with the function of preventing hair loss and strengthening hair as claimed in claim 2, characterized in that: In step (4), the material-liquid ratio of the Sophora japonica flower and cactus powder to water is 1:6-10 g / mL; the hot pressure distillation is carried out at 0.2-0.3 MPa and 80-100° C. for 2-4 hours; In step (5), the liquid ratio of the noni fruit enzyme, snake skin and water is 1:10-12 g / mL.

6. Use of the composition containing purple sweet potato leaf extract for preventing hair loss and strengthening hair as claimed in claim 1 in the preparation of a hair lotion product.

7. The use according to claim 6, characterized in that The preparation method of the hair lotion product comprises: (1) adding a composition containing purple sweet potato leaf extract into water, stirring and heating the water to above 70° C. to form a liquid medicine; (2) Amino acid thickener PEG-120, sodium lauroyl glutamate, cocamidopropyl betaine, NaCl, potassium sorbate, tea polyphenols, guar gum and aqueous azone are added to the liquid in sequence, stirred evenly, and water is added to 1000 mL to obtain the target hair wash product.

8. The use according to claim 7, characterized in that In step (1), the solid-liquid ratio of the composition containing purple sweet potato leaf extract to water is 1:5-7 g / mL.

9. The use according to claim 7, characterized in that: In step (2), the following raw materials are included in parts by weight: 25-35 parts of amino acid thickener PEG-120, 90-110 parts of sodium lauroyl glutamate, cocamidopropyl betaine, 18-22 parts of NaCl, 4-6 parts of potassium sorbate, 4-6 parts of tea polyphenols, 1-3 parts of guar gum and 9-11 parts of aqueous azone.

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