The invention relates to a method and a culture medium for improving the embryoid induction rate of eggplant anther culture, and belongs to the technical field of
plant tissue culture. The method comprises the following steps: (1) obtaining an embryoid; and (2) obtaining the regenerated haplobiont. The culture medium used in the method is an anther culture
solid culture medium and a healthy
seedling rooting
solid culture medium. The method has the following advantages that the steps of the culture method are simplified, the operation is convenient, the embryoid induction rate of eggplant anther culture can be effectively improved, and particularly the problem that the culture efficiency of recalcitrant eggplant varieties with poor anther culture response is low is solved; according to the method, the
embryo forming period is short, embryoid formation can be observed by naked eyes only in three weeks of anther
inoculation, the eggplant haploid breeding process is remarkably accelerated, and the breeding efficiency is improved.