45 results about "Porcine skin" patented technology

Collagen membranes are formed from porcine rinds (i.e., pig skins) for us in a variety of applications and, most preferably, for wrapping food products, such as hams and the like. First, after removing skins from the porcine, the skins are promptly frozen. In later processing, the rinds are thawed and then enzymatically defatted. Then, a quick alkalinic hydrolyzation is performed on the rinds. Then, an acidic hydrolyzation is performed on the rinds. The rinds are then ground into a gel-like fluid mass. Finally, the fluid mass is extruded, sheeted and dried into a collagen membrane. The collagen membrane produced can be, in preferred embodiments, used for wrapping food products, such as hams.

A process for preparing the biologic filler able to be injected in soft tissue for repairing it or beautifying skin includes such steps as providing the fresh skin of piglet, removing subcutaneous fat, immersing in defatting liquid for defatting, defatting again, alkali treating, removing alkali, cutting for layering, softening by enzyme liquid, cross-linking, water washing, bleaching, cutting to become particles, packing and Co-60 irradiation.

The invention discloses antigen-free porcine dermal collagen fibers and a preparation method thereof. The antigen-free porcine dermal collagen fibers with excellent performance and wide application are prepared by using purified porcine skin as a raw material and adopting a method of combining physics, chemistry and biochemistry to perform a series of treatment such as fluffing, fiber decomposition, fiber separation, carding and the like on the purified porcine skin on the premise of ensuring good mechanical property of the purified porcine skin unchangeable. The antigen-free porcine dermal collagen fibers have good biocompatibility, good mechanical property and proper degradability, and can be widely applied to preparation of medicinal biological materials such as tissue engineering scaffold materials, hemostatic materials, medicinal operation stitches and the like.

This invention relates to a moisture collagen protein face mask and its preparation method. This method is to extract the collagen protein from the fresh hogskin which is the functional material. It is made up of the 0.05-5% collagen protein, 0.2-10% cellulose derivant, the starch 0.05-10%, the moisture agent and the cross linker 1-20%. The process includes the reaction, the dry and forming. The face mask has not only the double moisture keeping efficient but good toughness, the ductility and adhesiveness; also it has the good feelness. So it is a good cosmetic.

The medicated diet with functions of replenishing collagen and nursing face is produced with pig trotter, cow trotter, pork skin and / or chicken toe, soybean, black bean, date, longan, tremellar, black fungus, black sesame, white sesame, peanut, wolfberry fruit, Dangshen, salt and water as material. Its production process includes the steps of washing the materials, boiling and stewing. It has effective health functions of replenishing collagen and nursing face, and wide material source.

The invention discloses a method for extracting pigskin collagen peptides. The method comprises the following steps that: fresh pig skin serving as a main raw material undergoes cleaning, water addition, addition of sodium sulfite and boiling, filtration, defatting of lipase, enzymolysis of protease, enzymic inactivation, decoloring and membrane separation, and the obtained filtrate is dried to form the pigskin collagen peptides. The method has the advantages of short production period, low cost, mild reaction conditions and environmental friendliness, and the products prepared by the method are high in safety and widely applied in the fields of healthcare products, cosmetics, medicines and the like.

The present invention discloses a new application of pig skin which can be substituted for human skin and its preparation method. Said preparation method includes the following steps: killing health living while pig with about 50 kg, shaving hair and cleaning pigskin, skinning and chipping to obtain skin sheet, cross-linking with glutaraldehyde, cleaning with balanced salt solution phosphate, soaking it in IM physiological saline containing trypsase, vibrating with Triton-100, more washing it with balanced salt solution phosphate and storing it in refrigerator with 4 deg.C for stand-by. Said invented product has the functional characteristics of two skin substitutes of Integra artificial skin and ADM, so that it not only can be used as temporary wound surface covering material for protecting surface of wound, but also can be used as permanent substitute for dermis for repairing surface of the wound.

The invention discloses a SgRNA for lowering immunogenicity, a low-immunogenicity dressing and a preparation method thereof. The sgRNA for lowering immunogenicity has a length from 18nt and 22nt; the3' end of the target sequence on the GGTA1 gene of the sgRNA contains GG, and the GC content is 40%-60%; The sgRNA does not have SNPs in the genome sequence of the target combination site; the sgRNA is compared with a pig GGTA1 gene to carry out the whole gene off-target effect analysis, and maximum 5 base mismatches are allowed in the off-target site. The low-immunogenicity dressing is pig skin,the pig skin is derived from a GGTA1 gene knockout pig. The sgRNA for lowering immunogenicity and the low-immunogenicity dressing have the advantages of low immunogenicity, even no immunogenicity, canbe used as a human skin substitute and reduce the risk of complications.

The invention relates to a circRNA related to subcutaneous fat of pigs and an application thereof, and specifically relates to a circRNA_11897 related to subcutaneous fat of pigs and the application thereof. The invention identifies and analyzes the circRNA of the subcutaneous fat of large white pigs and Laiwu pigs, adopts a systematic bioinformatic method for performing functional analysis on thedifferential expression circRNA and finds the circRNA_11897 and the genes ssc-miR-27a, ssc-miR-27b-3p and SCD related to the circRNA_11897. The experiment proves that the expression quantity of the circRNA_11897 and the genes has a close relation with the subcutaneous fat of pigs. The invention provides a new theoretical basis and a potential target for improving the quality of pork and preventing the happening of metabolic diseases caused by dyslipidemia.

The invention relates to a method for extracting ultralow molecular collagen. The method for extracting the ultralow molecular collagen is characterized by comprising the following steps of: 1, rinsing porcine skin or bovine skin by using water; 2, heating and stewing the cleaned porcine skin or bovine skin with water; 3, removing partial moisture from the low-concentration colloidal liquid through an evaporator; 4, adding 1 to 3 percent trypsin into the high-concentration colloidal liquid to perform enzymolysis; and 5, purifying and sterilizing the ultralow molecular collagen liquid, and drying the ultralow molecular collagen liquid into collagen powder through a spray dryer. The method for extracting the ultralow molecular collagen can keep the molecular weight of a large amount of collagen finished product below 3,000 Daltons and fully extract the collagen in the raw materials.

The production method of medical pork skin includes the following steps: a. raw material preparation; b. soaking the raw material in physiological salt solution; c. dewatering; d. soaking in cerium nitrate solution; e. double-layer vacuum packaging; f. using electronic uccelerator to make irradiation and disinfection; and g. freezing, putting into storage. Said invention adopts the cerium nitrate solution and electronic accelerator irradiation and disinfection, so that it raises the sterilizing disinfection effect of pork skin, raises its anti-infective power, and can delay repellency and can prevent growth of bacteria, and can shorten wound healing time, therefore it can obtain good effect in clinical application.

The invention discloses a novel bioengineering decellularized dermal matrix and a preparing method thereof. The preparing method includes the steps that porcine skin is pretreated to obtain dermal layer skin graft, and a mixed protective solution is used in all the dermal matrix decellularization process; a high hydrostatic pressure technology is used for preseparating cells in dermal tissue; residual cell nucleuses are digested through a composite nuclease method; detergent is used for removing loose smudge cell sheets; the mixed protective solution is used for rinsing. Suitable and efficient physical means are used and supplemented with a small amount of enzymes and detergent, cell components in the dermis are fully removed, under whole-process protection of the protective solution, it can be ensured that immunogenicity of the dermal matrix is reduced to the maximum on the basis of ensuring complete removal of the cell nucleuses, a normal three-dimensional collagenous fiber structure and array polarity of the dermal matrix are reserved, and therefore the prepared decellularized dermal matrix is high in stability and biocompatibility.

The invention discloses a method for making purified pigskin. In the method, healthy and fresh pigskin is taken as raw material, the pigskin is repeatedly cleaned without changing the three dimensional structure of the pigskin, multiple enzymes and basic materials are adopted to treat the pigskin alternately, and non collagen ingredients and small molecular substances in the pigskin are removed, thus obtaining the purified pigskin with favourable biocompatibility and bioactivity. The purified pigskin can be used for extracting medical collagen, preparing tissue engineering support material and repairing and reconstructing material.

The present invention belongs to the field of medical accessory bioengineering. The sterilized heterogeneous medical biomembrane is sterilized piglet skin and has a thickness of 0.5-1.0mm. The invention is characterized in that a plurality of gaps are distributed on the surface. Compared with the prior art, the sterilized heterogeneous medical biomembrane of the invention has a beneficial effect that the piglet skin which is a heterogeneous skin with optimal biocompatibility to the human body is selected. Appropriate thickness and the designing of gaps cause that the rewarming is quick and the using is convenient in application. The same sterilization effect can be obtained when an electron accelerator of nuclear reactor is adopted for irradiating with a time shorter than that of the irradiation time of Cobalt-60. Furthermore no katogene exists for the dermal cells and fibrous tissue of biomembrane.

The invention provides a construction method of immortalized cell lines of porcine subcutaneous adipose precursor cells. The method includes the following steps that primary porcine subcutaneous adipocytes are obtained; cells are cultured, and pBABE-puro-hTERT plasmids are transfected when the cell growth density is 60%-70%; after transfection for 48+ / -2 h, a 30%-serum DMEM medium containing 4 microgram / mL purinomycin used for screening; after screening for 48+ / -2 h, and liquid is changed for cultivation of the DMEM medium; after cells are cloned, the cloned cells are digested and cultured, and identification is performed until the immortalized porcine subcutaneous adipose precursor cell model is obtained. The immortalized cell lines of porcine subcutaneous adipose precursor cells can be used for adipose precursor cell proliferation, or polyester mechanism differentiation and regulation mechanism research.

The production process of medicinal pig skin includes the following steps: soaking pig skin in degreasing solution, comprising soda, lime and water, for 0.5-4 hr to degrease; soaking the washed pig skin in medicine solution comprising Norfloxacin and water for 8-14 hr to medicate; and irradiating the treated pig skin with Co-60 ray. The pig skin thus treated is bacteria-free, has obvious antiseptic effect, less rejection and less protein and water loss, and may be used clinically.

The present invention provides a fetal pig skin precursor tissue for repairing the skin wound. The fetal pig skin precursor tissue is originated from the inbred strain of all pig species, the fetal pig of the blocked group and the fetal pig of the hybridization breeds. The gestational age of the fetal pig is from 21 days till the birth day, which comprises direct obtaining the fetal pig skin precursor tissue or the fetal pig skin precursor tissue after gene modification. The xenotransplantation is conducted after preparing the tissue into the shape of the cell suspension, the cell mass, the particle or the stamp skin or the big piece of skin. Under proper microenvironment, the present invention comprises the cleaning, the low cell immune response ability, induction of the immune tolerance between the host and the implant (fetal pig skin) or modification in the pig skin precursor tissue. The skin precursor tissue provided in the present invention can grow and develop into a complete skin with the dermis, the epidermis and the appendages of the skin. The present invention can be used in repairing the skin defect caused by various kinds of reasons. The present invention can promote the wound healing; therefore a complete skin is formed.

The invention discloses a preparation method of decellularized porcine dermal matrix dressing by using pork rind. The preparation method is characterized in that a physical method, a chemical method and a biochemistry method are adopted, a freeze drying technique is adopted, under the premise that a three-dimensional network structure of the pork rind is maintained unchanged, through the steps ofdegreasing, removing hair, alkali treatment for inactivation of virus, decellularizing, cross-linking, post-pretreatment, irradiation sterilization and the like, antigenic substances in the pork rindcan be effectively and thoroughly cleared, the shape of a hypodermal cell epimatrix is completely reserved, and the properties of the physical intensity, the hydrophilicity, the appropriate degradability, the storage stability and the like of the materials are improved.

The invention relates to a method for high-efficiency in-vitro separation culture of porcine skin-derived stem cells. The method comprises the following steps of cutting off adult porcine skin, removing hair, fat and dirt of the adult porcine skin, immersing the treated adult porcine skin in alcohol having the content of 75%, cleaning the adult porcine skin by PBS, cutting the adult porcine skin into small porcine skin pieces, carrying out digestion in 0.25%Trypsin at a temperature of 4 DEG C until the next day, cleaning the porcine skin pieces by a DMEM high-glucose culture solution, carrying out digestion by 0.25%Trypsin-0.04%EDTA, carrying out blowing beating, carrying out screening by a cell sieve of 400 meshes to obtain a cell suspension, transferring the cell suspension into a porcine skin-derived stem cell culture solution, carrying out culture, cleaning the adult porcine skin cells subjected to primary culture for 3-4 days by PBS, adding 0.25%Trypsin-0.04%EDTA into the culture products, carrying out rinsing and digestion, tapping a side wall of a dish so that the cells fall off, transferring the cell suspension subjected to digestion into a centrifuge tube, carrying out centrifugation, removing a supernatant, carrying out cell re-suspension, and carrying out passage. The method has simple and convenient processes and good repeatability. The porcine skin-derived stem cells obtained by the method can express beta1-integrin and Nestin skin-derived stem cell specific proteins and are alkaline phosphatase-positive cells.

A preparation method of pig or cow bladders membrane biological dressing used in treatment of burns. It relates to a preparation method of pig or cow bladders as a biological dressing in order to provide new biological dressing product and belongs to preparation of medical technology field. The preparation steps are: extract bladder, clean, sort, and aerate, cut, disinfect, and chill. The pig or cow bladder membrane biological dressings prepared by this way can meet the operating requirements of burns in the meanwhile the products are low-cost owing to its no requirement for special equipments in preparation process. Contrast to pigskin dressing, the pig or cow bladder membrane biological dressings are relative good and inexpensive. As preparation method comes out, use pig or cow bladder as the biological dressing in burn treatment can be carried out, the medical costs for burned patients also reduced significantly and a better curative treatment superior to pigskin treatment can be achieved and it has made contributions to the development and technology progress to the burn treatment.

A feature-beautifying liquid collagen honey is prepared through a reaction of collagen in pigskin and wild honey according to a certain proportion, is a pure natural drug liquid, and does not have any chemical substances. The drug liquid collagen is extracted from the pigskin; during extraction, the collagen in the pigskin can be converted into gelatin in a cooking process; gelatin has a netty space, can be combined with a lot of water, enhances cell physiological metabolism, and effectively improves body physiological functions and a water storage function of skin tissue cells, so that cells are moistened and are kept at a moist state, skin is prevented from premature wrinkling, and the aging process of the skin is delayed; moreover, the honey also has skin-care and feature-beautifying effects and has long shelf life. Therefore, the extracted pigskin collagen and the honey are subjected to mixing and reacting according to a certain proportion, and a soft body like jelly is generated, namely the feature-beautifying liquid. The drug liquid is convenient to use, safe and environmentally friendly, and can be both oral and externally applied. Externally application is equivalent to exoroot fertilization of plants.

The invention relates to a method for depilating pigs, belonging to the field of animal husbandry and slaughtering, and is used for slaughtering and processing live pigs. A method of depilation of pigs, depilation with water scalding. The scalding water temperature is controlled at 56-58 ℃ in summer, and the scalding time is 4-6 minutes; in winter, it is 58-61 ℃, and the scalding time is 12-15 minutes. The scalding time and water temperature in spring and autumn are between winter and summer. After mechanical depilation, pour the heated food-grade rosin oil ester (adding a certain proportion of lard suet) onto the surface of the pig for re-hair removal (heating with a rosin heating pot). The oil ester is manually smashed and played back to the rosin heating pot for repeated heating. Using the method of the present invention for hair removal, the average hair removal rate of Huai pig commercial pigs is 98%. After knife trimming, the broken hair root in pig body skin was less than 2%. Pig skin is white and neat.

The invention discloses a derived scaffold based on a skin-derived acellular matrix and a construction method of the derived scaffold. Through a decellularization technology, firstly, pigskin is subjected to decellularization treatment through a pancreatin method, a Triton-X-100 method and a DNase method in sequence, then a freeze-dried decellularization matrix is digested through pepsase, then the decellularization matrix is mixed with gelatin and chitosan in a matching mode, and the porous three-dimensional acellular matrix derived scaffold is obtained through freeze drying. The pigskin-derived acellular matrix is selected as the matrix material, and the gelatin and chitosan are compounded to prepare the composite porous scaffold, so that advantage complementation of the functions of the materials is realized. The pigskin is wide in source and low in cost, and meanwhile, the extracellular matrix is a substance synthesized and secreted in the growth process of cells and can provide a natural growth microenvironment of fibroblasts to the maximum extent; the gelatin is compounded to improve the mechanical strength of the scaffold and increase the cell adhesiveness of the scaffold; the chitosan is compounded to improve the mechanical strength and water absorption of the scaffold and endow the scaffold with broad-spectrum antibacterial performance. The scaffold can be used as an excellent biomedical material in the field of skin tissue engineering.

Bone jelly with tomato is made from the following materials according to parts by weight: 30-35 parts of tomato, 10-11 parts of edible salt, 3-4 parts of sodium glutamate, 25-30 parts of porcine bone, 1-1.5 parts of lactic acid bacteria powder, 6-8 parts of glucose, 15-20 parts of porcine skin, 3-4 parts of anise, 3-4 parts of fennel, 1-2 parts of chicken extract, 1-1.5 parts of Radix Ophiopogonis, 0.5-1 part of Radix Scrophulariae, 0.5-1 part of Caulis Dendrobii, 1-2 parts of Radix Polygonati Officinalis and the like; as to the bone jelly with tomato of the invention, natural meat skin as jelly material is made into a jelly condiment that melts easily, is rich in nutrition and delicious in taste, whole tomato juice and bone powder are added to the condiment to provide rich vitamins and calcium, and in collocation with traditional Chinese medicine extracts from such as Radix Platycodonis, Radix Ophiopogonis and Radix Scrophulariae, the effects of tonifying yin and nourishing blood and tonifying iron are achieved; broken-wall yeast powder is used to improve delicacy and as a condiment, and good taste is brought.

The invention discloses moist essence collagen milk (2). Fresh porcine skins are cleaned and heated after fat is removed, collagen (1) in the porcine skins is extracted at high temperature after the fat is completely removed, and then the collagen (1) and wild honey react according to a certain proportion to prepare the moist essence collagen honey. The moist essence collagen honey is a pure natural liquid medicine free from any chemical substances. The collagen (1) of the liquid medicine can enhance cell physiological metabolism and effectively improve body physiological functions and water storage functions of skin tissue cells, so that the cells are moistened and kept in a moisture state, skins are prevented from prematurely wrinkling, the aging process of the skins is delayed, honey has skin care and cosmetic functions and long shelf lives, and collagen protein (1) extracted from porcine skins and the honey are mixed and reacted according to a certain proportion to generate soft body like jellies to obtain moist essence (2). The liquid medicine is convenient to use, safe and environmentally friendly, can be taken orally and can also be externally applied (external application is equivalent to foliage fertilization of plants).

The invention discloses a method for constructing a skin nonlinear constitutive model under the action of a compression load. The method comprises the following steps: establishing an improved Odgen model as a skin hyperelastic part model; establishing a Maxwell model as a relaxation unit to describe a relaxation phenomenon caused by skin viscoelasticity; establishing a standard three-parameter solid model as a creep unit to describe a creep phenomenon caused by skin viscoelasticity; by introducing a strain rate function, a specific model capable of describing skin mechanical behaviors under the strain rate of 10 <-4 > / s-10 < 3 > / s is established; by utilizing the pigskin nonlinear constitutive model provided by the invention, the strain rate sensitivity of the pigskin and relaxation and creep phenomena caused by viscoelasticity of the pigskin can be captured, and a foundation is laid for subsequent modeling and simulation of skin materials.