A kind of preparation method of heterogeneous acellular dermal matrix

Abstract

The invention relates to a preparation method of a heterogeneous acellular dermal matrix. Fresh salted pigskin is used as a raw material, and the method of combining physics, chemistry and biochemistry is used to degrease, alkali-swell, eliminate alkali-swell and treat with keratinase , secondary enzyme treatment and a series of treatments to prepare a heterogeneous (pig) acellular dermal matrix with excellent performance and wide application. The method adopts keratinase treatment and secondary enzyme treatment, and the method has the advantages of less environmental pollution and the heterogeneous (pig) acellular dermal matrix treated by the method has a complete three-dimensional structure of collagen, good water vapor permeability, high porosity, The material has excellent mechanical properties and can be widely used in the fields of burns and plastic surgery.

Description

technical field [0001] The invention relates to the field of bioengineering, more specifically, to a method for preparing heterogeneous (pig) acellular dermal matrix Background technique [0002] Acellular matrix (acellular dermal matrix, ADM) is obtained by using physical, chemical, biological and other methods to completely remove the epidermis and cells containing antigen components in the skin, leaving only the extracellular matrix containing collagen in the dermis. Wound coverage, tissue defect filling, guiding tissue regeneration, etc. Because ADM has no cellular components at all and low immune activity, it generally does not induce immune responses. However, ADM retains the three-dimensional structure of normal collagen and the extracellular matrix containing collagen scaffolds in the dermis. These components can support the growth of the split skin and are also conducive to the adhesion and proliferation of seed cells. Therefore, the acellular matrix is ​​widely us...

AI Technical Summary

Problems solved by technology

[0003] Porcine acellular dermal matrix (PADM) prepared by traditional methods has disadvantages such as incomplete cell removal, poor permeability, low porosity, and low tensile strength.

The Chinese patent No. CN1387923 discloses a heterogeneous acellular dermal scaffold and its preparation method. The scaffold is obtained by treating suckling pig skin with trypsin and glutaraldehyde. The dermal scaffold treated by this method has tensile strength High and good biocompatibility, but its permeability is poor, the wound is prone to fluid accumulation, gas accumulation, slow vascularization, and glutaraldehyde is toxic to cells, which is potentially dangerous

The Chinese patent with the patent number CN1343523 discloses a microporous allogeneic or heterogeneous acellular dermal substitute that can be used for wound repair, using mechanical or laser methods to regularly make dense penetrating holes on the acellular dermis. Although it can solve the shortcomings of poor permeability of acellular dermal substitutes, too large or too dense pore size will increase the exposure of antigenic sites and the chances of antigen-presenting cells contacting ADM, resulting in poor cell compatibility and a certain degree of disrupt the structure of the collagen scaffold

Images