Preparation method of decellularized porcine dermal matrix dressing by using pork rind

Abstract

The invention discloses a preparation method of decellularized porcine dermal matrix dressing by using pork rind. The preparation method is characterized in that a physical method, a chemical method and a biochemistry method are adopted, a freeze drying technique is adopted, under the premise that a three-dimensional network structure of the pork rind is maintained unchanged, through the steps ofdegreasing, removing hair, alkali treatment for inactivation of virus, decellularizing, cross-linking, post-pretreatment, irradiation sterilization and the like, antigenic substances in the pork rindcan be effectively and thoroughly cleared, the shape of a hypodermal cell epimatrix is completely reserved, and the properties of the physical intensity, the hydrophilicity, the appropriate degradability, the storage stability and the like of the materials are improved.

Description

technical field [0001] The invention belongs to the processing field of biomedical materials, and in particular relates to a preparation method of pigskin decellularized dermis matrix dressing. Background technique [0002] In the past two decades, the research and development of materials for the treatment of skin defects has progressed rapidly, mainly in two directions, one is the treatment of autologous skin or allogeneic donated skin, and the other is the treatment of heterogeneous skin or organs, including the skin of cattle, sheep and pigs. , tendon, visceral membrane, intestinal mucosa, etc. Several companies in China have obtained relevant registration certificates and used them in actual clinical practice. [0003] The main component of pigskin acellular dermal matrix dressing is collagen (fiber), which has good physical and mechanical properties, weak immune antigenicity, biodegradability and absorbability, good interaction with cells, and good hemostatic performa...

AI Technical Summary

Problems solved by technology

[0006] 1. Manual operation, labor intensity is too high;

[0007] 2. There may be hair roots and other impurities in the mass-produced matrix materials;

[0008] 3. The physical strength is low, because the glutaraldehyde crosslinking agent is highly toxic, the dosage should not be high, and the treatment time should not be long, not more than 2 hours. If it is too high or too long, it will cause the material to turn yellow and become brittle, so it acts as a disinfectant in normal times. Function, the processing time is about 30 minutes, and this time is too short, it can not achieve the actual effect of cross-linking to improve the physical strength, and the low physical strength will cause the material to be easily torn during the stretching process, and it is easy to degrade and break during the treatment of covering the wound , after the scab is incompletely shed due to disintegration, the thickness is not uniform enough, and it is easy to rot and smell after use;

[0009] 4. Wet storage is not safe, and it needs to be preserved with preservatives, etc.

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