Preparation method of acellular dermal matrix material

A technology of acellular dermis and matrix materials, applied in the field of preparation of acellular dermis matrix materials, which can solve problems such as incomplete crosslinking, material tearing, and low physical strength, so as to improve physical and mechanical properties and reduce biodegradability , the effect of high degree of mechanization

Inactive Publication Date: 2019-02-22
JIANGYIN BENXIANG BIOTECHOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] 1. Manual operation, labor intensity is too high;
[0008] 2. Degreasing, hair removal, virus inactivation, cross-linking and other processes are incomplete, and the product may contain fat and hair roots;
[0009] 3. The physical strength is low, because the glutaraldehyde crosslinking agent is highly toxic, the dosage should not be high, and the treatment time should not be long, not more than 2 hours. If it is too high or too long, it will cause the material to turn yellow and become brittle, so it acts as a disinfectant in normal times. Function, the processing time is about 30 minutes, and this time is too short, it can not achieve the actual effect of cross-linking to improve the physical strength, and the low physical strength will cause the material to be easily torn during the stretching process, and it is easy to degrade and break during the treatment of covering the wound , after the scab is incompletely shed due to disintegration, the thickness is not uniform enough, and it is easy to rot and smell after use;

Method used

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  • Preparation method of acellular dermal matrix material

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Embodiment 1

[0039] A method for preparing an acellular dermal matrix material, comprising the following steps, wherein, unless otherwise specified, the amount of the raw and auxiliary materials is expressed as the weight percentage of the processed weighed tomographic skin slice, that is, the weight of the raw and auxiliary materials = weight of sliced ​​skin piece processed × percentage:

[0040] (1) After slaughtering and skinning healthy pigs, trim the edges, and remove excess floating meat on a hydraulic meat remover. After washing with a large amount of water, use a skinning machine to cut the animal skin into a split skin with a thickness of 0.3-1.0mm. The slices are weighed, and then these slices are sequentially processed in the drum as follows.

[0041] (2) Chemical degreasing. Set the drum temperature to 31°C, add 150% water, 2% leveling agent, 35% degreasing agent, rotate at a constant speed for 60 minutes, and dry the waste liquid. And after washing twice with water, repeat ...

Embodiment 2

[0049] A method for preparing an acellular dermal matrix material, comprising the following steps, wherein, unless otherwise specified, the amount of the raw and auxiliary materials is expressed as the weight percentage of the processed weighed tomographic skin slice, that is, the weight of the raw and auxiliary materials = weight of sliced ​​skin piece processed × percentage:

[0050] (1) Select healthy sheep, after slaughtering and peeling, trim the edges, remove excess floating meat on a hydraulic meat remover, wash with a large amount of water, and cut the animal skin into slices with a thickness of 0.3-1.0mm with a skinning machine The skin slices are weighed, and then these sliced ​​skin slices are sequentially processed in the drum as follows.

[0051] (2) Chemical degreasing. Set the temperature of the drum at 30°C, add 150% of water, 2% of Pingping, and 5% of degreasing agent, rotate at a constant speed for 120min, and control the waste liquid. And after washing twi...

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Abstract

The invention discloses a preparation method of an acellular dermal matrix material. The preparation method of the acellular dermal matrix material is characterized in that removing flesh and hair from an animal skin, then taking a split-skin graft, and carrying out defatting, unhairing, inactivation of virus, cell extraction, crosslinking modification, cleaning and moisturizing, packing and irradiation sterilization processes to obtain the acellular dermal matrix material. The preparation method of the acellular dermal matrix material has the characteristics that the process time is short, aneffect of removing antigen substances is good, and the material has quite good adhering property, moisture retention property, breathability and the like.

Description

technical field [0001] The invention relates to a method for preparing an acellular cortical matrix material, belonging to the field of biomedical material preparation. Background technique [0002] In the past two decades, the research and development of materials for the treatment of skin defects has progressed rapidly, mainly in two directions, one is the treatment of autologous skin or allogeneic donated skin, and the other is the treatment of heterogeneous skin or organs, including the skin of cattle, sheep and pigs. , tendon, visceral membrane, intestinal mucosa, etc. Several companies in China have obtained relevant registration certificates and used them in actual clinical practice. Regarding the preparation of acellular dermal matrix materials, related invention patents have also been disclosed in recent years, such as: [0003] Invention patent application 201710682043.6 discloses a preparation method of acellular dermal matrix, which includes the following steps:...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/50
CPCA61L27/362A61L27/3687A61L27/3691A61L27/507A61L2430/34
Inventor 黄盛丛亮亮刘汝诚
Owner JIANGYIN BENXIANG BIOTECHOLOGY
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