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Farrow skin prosoma organization for renovating skin wound, preparing method and use method thereof

A technology for repairing skin and fetal pigs, applied in prosthetics, medical science, etc., can solve the problems of not being able to completely overcome the risk of teratoma formation in embryonic tissue, and fail to achieve satisfactory results, so as to reduce scar formation and prevent Infection, the effect of improving the prognosis

Inactive Publication Date: 2008-03-12
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a large number of previous experimental studies have not been able to completely overcome the risk of embryonic tissue formation of teratomas
In particular, preclinical experiments on large animal models have not achieved satisfactory results

Method used

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  • Farrow skin prosoma organization for renovating skin wound, preparing method and use method thereof
  • Farrow skin prosoma organization for renovating skin wound, preparing method and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Acquisition and preparation of fetal pig skin precursor tissues of Guizhou Xiaoxiang pigs with gestational ages E42 and E55

[0029] 1. Anesthesia: female Guizhou miniature pig sows with accurate gestational age (E42 and E55), 3% sodium pentobarbital: total dose 1ml / kg, injected into the ear vein in divided portions; half the amount for the first time, divided into 2-3 times The interval between injections is about 15 minutes, and it can be increased or decreased according to the specific situation.

[0030] 2. Make an opening in the middle of the back of the abdomen. The length of the incision is about 8cm. Open each layer layer by layer and enter the abdominal cavity.

[0031] 3. Carefully turn out the uterus, touch the bulge of the uterus (with fetal pigs) with your hands, incise the uterus with a small incision to take the fetus, ligate the umbilical cord, and suture the full thickness of the uterine wall with blanket suture.

[0032] 4. Wash the fetal p...

Embodiment 2

[0037] Example 2: BABL / c nude mouse transplantation of fetal skin precursor tissue of Guizhou Xiaoxiang pigs at gestational ages E42 and E55.

[0038] 1. Anesthesia: Mouse weight 15-18g / mouse, 1% pentobarbital sodium 0.1ml ip, if wake up during operation, add 0.02ml / mouse as appropriate. (According to the total dose of 5-8μl / g body weight).

[0039] 2. Fixation: Fix the nude mice with sterile medical adhesive tape.

[0040] 3. Disinfection: Disinfect the back and double auricles of nude mice with povidone iodine.

[0041] 4. Subcutaneous puncture of the auricles: Use a 12-gauge lumbar puncture needle to perform subcutaneous puncture at the middle and upper 1 / 3 of the dorsal sides of the bilateral auricles from the distal end to the proximal end. The fetal pig microskin outside the needle hole space at the front end of the 12-gauge lumbar puncture needle was implanted under the skin of the pinna.

[0042] 5. Open with a scalpel on the back: 1cm×1.5cm or 1cm×2cm, full-thickne...

Embodiment 3

[0046] Embodiment 3: Observing the growth after transplantation (recording the general changes of the wound surface and the subcutaneous surface of the auricle).

[0047] 1. Changes of back wound after E42 transplantation. After the transplantation of fetal pig skin precursor tissue of E42, the wound on the back of nude mice is covered by allogeneic skin, which will turn black within a week and may be accompanied by blisters, etc. After one week, the allogeneic skin is almost completely necrotic, forming a dry scab. On the wound surface; 3-4 weeks, some subcutaneous bulges appeared successively at the junction of the dry scab of the allogeneic skin and the normal skin, the number was small and not obvious, the surface layer of normal skin was well covered, and could slide with the skin; 4-5 weeks, the bulges further increased , the number increased, obviously visible, the bulge was solid, round, and protruded on the back in a disc shape, and the subcutaneous dark shadow could ...

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Abstract

The present invention provides a fetal pig skin precursor tissue for repairing the skin wound. The fetal pig skin precursor tissue is originated from the inbred strain of all pig species, the fetal pig of the blocked group and the fetal pig of the hybridization breeds. The gestational age of the fetal pig is from 21 days till the birth day, which comprises direct obtaining the fetal pig skin precursor tissue or the fetal pig skin precursor tissue after gene modification. The xenotransplantation is conducted after preparing the tissue into the shape of the cell suspension, the cell mass, the particle or the stamp skin or the big piece of skin. Under proper microenvironment, the present invention comprises the cleaning, the low cell immune response ability, induction of the immune tolerance between the host and the implant (fetal pig skin) or modification in the pig skin precursor tissue. The skin precursor tissue provided in the present invention can grow and develop into a complete skin with the dermis, the epidermis and the appendages of the skin. The present invention can be used in repairing the skin defect caused by various kinds of reasons. The present invention can promote the wound healing; therefore a complete skin is formed.

Description

technical field [0001] The present invention relates to a source of xenogeneic skin transplantation for wound repair, specifically, the present invention relates to the use of fetal pig skin precursor tissue of a certain gestational age for repairing wound defects caused by various reasons and rebuilding complete skin, To achieve a real physiological restoration. Background technique [0002] The wound problem is the core issue in the treatment of severe burns and trauma. There is a serious shortage of autologous skin sources. Allogeneic and xenogeneic skins with mechanical and physiological protective effects are currently biological dressings that cover wounds and temporarily simulate skin functions. However, adult allogeneic or xenograft skin transplantation needs to overcome strong immune rejection. Although the survival time of allogeneic or xenograft can be prolonged by inhibiting rejection and inducing immune tolerance, it is still rejected by the recipient eventuall...

Claims

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Application Information

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IPC IPC(8): A61L27/60
Inventor 吴军黄正根罗高兴程文广贺伟峰
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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