9 results about "Miniature pig" patented technology

This invention discloses an intestinal fungus Saccharomyces cerevisiae M7015 and its applications belong to the field of microbial technology. Saccharomyces cerevisiae The accession number for M7015 is GDMCC No. 66195. This invention first demonstrated, using a mouse subcutaneous stroma embolism model, that the aforementioned strain can promote angiogenesis; secondly, using mouse and Bama miniature pig myocardial infarction models, it was found that the aforementioned strain can promote collateral angiogenesis in ischemic areas of the heart and improve cardiac function. This invention not only fully elucidates... Saccharomyces cerevisiae M7015 promotes the formation of collateral vessels in the heart and improves cardiac function, and also reveals the broad application prospects of this bacterium in the treatment of vascular diseases.

This application relates to the technical field of biological gene detection, specifically disclosing a miniature pig SNP molecular marker combinatorial system and its application. The miniature pig SNP molecular marker combinatorial system disclosed in this application includes at least one of 221 SNP molecular markers. The physical locations of these 221 SNP molecular markers are determined based on sequence alignment during 10× coverage whole-genome sequencing of miniature pigs. The site information of the 221 SNP molecular markers is shown in Table 1. The miniature pig SNP molecular marker combinatorial system provided in this application is used for detecting SNP genotyping in miniature pigs and possesses novel, stable, cost-effective, and efficient properties.

The invention discloses application of water microspheres of modified polysaccharide in promoting wound skin regeneration and inhibiting wound skin fibrosis. In the invention, compared with a Control group (silicone oil) as a control and an unmodified polysaccharide water microsphere group (AVBEC), when the modified polysaccharide water microsphere group (AVBER) is externally applied to the skin of a bama miniature pig in a deep II-level scald model, the wound healing can be effectively promoted, the inflammation degree and duration can be controlled, and the extracellular matrix composition and plasticity can be regulated; expression of fibrosis marker protein is reduced, and the dual effects of promoting skin regeneration and inhibiting fibrosis are achieved. The problems that natural extracts and common polysaccharide microsphere products are low in biological activity and poor in stability are solved, and new functions of modified polysaccharide are found.

An experimental miniature pig rearing cage includes a rearing cage, a support base, and a heater. The rearing cage has a first switch door on its outer side and a second switch door on one side. The lower part of the rearing cage is connected to the support base, and the upper part of the support base has a fixed base. The lower part of the rearing cage has a lying board, and the lower part of the lying board is connected to the fixed base. The lying board has a heating chamber inside, and a heating pipe is connected to the inner side of the heating chamber. The heating pipe passes through the lying board and connects to the heater. The bottom of the heater is connected to the support base. The lower part of the rearing cage has multiple drainage troughs. Before use, the cage is disinfected, improving the survival rate of miniature pigs. The temperature of the miniature pigs' living environment can be adjusted according to needs. The cage is simple to operate, has a reasonable structure, and can achieve centralized collection of feces, reducing the probability of disease in miniature pigs and facilitating their rearing.

The application discloses a method for establishing a Bama miniature pig spleen deficiency and dampness excess model, and belongs to the technical field of animal models. The method is to reduce the immunity of the body by intraperitoneal injection of cyclophosphamide, and then to stimulate the Bama miniature pig by using high-sugar and high-fat feed and senna leaves in combination with a high-humidity external environment. After 8 weeks, the Bama miniature pig has the clinical manifestations of the spleen deficiency and dampness excess, such as body fatness, fatigue and laziness, white and slippery tongue fur, and loose and watery stool, and the model is verified in the reverse direction by using the classic compound Shenglin Bai Zhusan. The Bama miniature pig spleen deficiency and dampness excess model is applied to the research of human spleen deficiency and dampness excess and the development of spleen-invigorating and dampness-expelling drugs. The model provides a new model for standardizing, standardizing and scientifically evaluating the research of spleen-invigorating and dampness-expelling traditional Chinese medicines, and has the advantages of good practicability, strong conformity, simplicity and objectivity, and conforms to the guidance of the theory of traditional Chinese medicine syndromes, and has a wide application prospect.

An experimental miniature pig farrowing crate includes a farrowing crate, a slatted floor, a fixed enclosure, a feeding trough, a ramp, a movable enclosure, a fixing frame, and locking rods. The ramp is connected to the front of the farrowing crate, which has a storage cavity inside. A discharge pipe is located at the rear of the farrowing crate and communicates with the storage cavity. The discharge pipe is connected to a nut via a threaded connection. The fixed enclosure is connected to the upper part of the farrowing crate. The front of the fixed enclosure is connected to a main enclosure door via a shaft, and the sides of the fixed enclosure are connected to secondary enclosure doors via shafts. The fixed enclosure is connected to the feeding trough, which is connected to the farrowing crate. The feeding trough has a water trough and a feed trough. A sow activity area is located in the middle of the farrowing crate, and piglet activity areas are located on both sides of the farrowing crate. The farrowing crate has an installation groove that is adapted to the slatted floor and is connected to the slatted floor.

This invention discloses the application of the AKR1C1 gene and its encoded protein in breeding pig breeds with superior metabolic function. It belongs to the field of genetic engineering technology, and particularly relates to the application of the AKR1C1 gene in breeding pig breeds with superior metabolic function. The nucleotide sequence of the AKR1C1 gene is shown in SEQ ID NO.1. The amino acid sequence of the protein encoded by the AKR1C1 gene is shown in SEQ ID NO.2. This invention provides the AKR1C1 gene of the Guangxi Bama miniature pig, clarifies the nucleotide sequence of the AKR1C1 gene and the amino acid sequence of the encoded protein, and provides the application of the AKR1C1 gene in regulating bile acid metabolism in pig liver, providing a new molecular target for pig genetic breeding and contributing to the breeding of pig breeds with superior metabolic function.

The invention relates to the technical field of animal model construction, and particularly discloses a pregnancy intrauterine cochlea drug delivery animal model and a construction method and application thereof, and the construction method of the animal model provided by the invention comprises the following steps: establishing a pregnant mouse and Bama miniature pig model; under the guidance of an ultrasonic imaging system, in-vitro visual guidance is carried out on an intrauterine embryo cochlea injection path during pregnancy, an injection device is inserted into an embryo cochlea area, and drugs such as a gene carrier are injected into a target area through an ultrasonic guidance injection device, so that the intrauterine cochlea fixed-point administration animal model during pregnancy is obtained. According to the invention, the drug delivery of the embryo cochlea area is realized in a safer, faster and less-damage manner by combining the ultrasonic guidance technology. The animal model can be used for establishing drug therapy, gene knockout, supplementation and overexpression at the intrauterine embryo stage during pregnancy and simulating a technical scene for preclinical research of prenatal gene therapy of hereditary hearing loss.

The invention relates to the technical field of biomedical experimental animal models, in particular to a drug induction-based miniature pig diabetes mellitus model construction method and application. The drug-induced miniature pig diabetes model construction method comprises the following steps: mixing a citric acid solution with a sodium citrate solution to obtain a mixed solution, and adjusting the pH value of the mixed solution to 4.2-5.0 to obtain a citric acid buffer solution; under the dark condition, streptozotocin is dissolved in the citric acid buffer solution, and a streptozotocin solution is obtained; the prepared streptozotocin solution is injected into the ear edge vein of the fasting miniature pig at a time, and construction of the miniature pig diabetes mellitus model is completed; the injection dosage in the step S3 is 140 to 160 mg / kg. The construction steps of the miniature pig diabetes model are simplified, the long-term stable miniature pig diabetes model can be constructed, and a simpler, more convenient and more stable model construction method is provided for research on a miniature pig diabetes disease mechanism and a treatment method.