110 results about "Miniature pig" patented technology

The invention discloses a method for breeding Bama miniature pigs. The method includes the steps that pregnant sows are fed by postconceptual age fetus growth fodder from the 92 day of pregnancy to the day of farrowing, and the postconceptual age fetus growth fodder comprises, by weight, 40-70 parts of corn, 10-30 parts of rice bran, 25-40 parts of black bean pulp, 10-20 parts of fish protein concentrate, 15-25 parts of sweet potato leaves, 30-50 parts of hair weeds, 0.5-5 parts of rhizoma atractylodis macrocephalae, 0.5-5 parts of radix scutellariae, 3-8 parts of massa medicata fermentata, 5-10 parts of radix salviae miltiorrhizae, 5-10 parts of rhamnus aurea and 0.5-2 parts of plant extract, wherein the plant extract is a water solution containing 3% of cinnamyl aldehyde, 4% of mannan oligosaccharide and 5% of carvacrol. According to the method for breeding the Bama miniature pigs, the growth activity of piglets and the reproductive performance of the sows after farrowing can be improved obviously, and the healthy growth of the Bama miniature pigs at the later period can be promoted.

The invention discloses a large-scale ecological grazing pig cultivation method, and belongs to the technical field of animal husbandry cultivation. A female miniature pig is hybridized with a female Taihu pig; a hybridized first generation young sow is selected as a female parent, and hybridized with two male black pigs as male parents; and the hybridized second generation is ecologically cultivated. A luxuriant grass is taken as a sports ground type grazing district; renascent herbs are mixed sown on the grass; various pastures are mixed sown on the grass every year; an ecological hog house is built; strains, fermented grass and natural nutrition healthcare pills or the like are prepared; when the pigs are cultivated, one or more of black wheat, cyperus esculentus, prepared soybean, endive, alfalfa, fermented grass meal and natural nutrition healthcare sugared pills are fed to the pigs in the hog house in variable interval; after the pigs freely eat, the pigs are driven to the sports yard to graze in a short time. Various pastures are planted on the sports yard until the sports grass per Mu can allow more than 100 pigs to be cultivated, and thus the large-scale ecological cultivation is achieved.

The invention discloses a pig house washing device for cultivation of Congjiang miniature pigs. The pig house washing device comprises a base plate. Two fixing seats are fixed to the top of the base plate symmetrically, and second sliding grooves are formed in the adjacent lateral sides of the fixing seats respectively; a second sliding block is slidably mounted in each second sliding groove, anda lifting plate is fixedly connected between the two second sliding blocks; a first chain transmission mechanism is mounted at the bottom of the lifting plate, and comprises a first motor and a firstrotating shaft; a first driving chain wheel is mounted on an output shaft of the first motor, the first rotating shaft penetrates the lifting plate, and the first rotating shaft and the lifting plateare connected rotatably; a first driven chain wheel is mounted on the first rotating shaft, and a first chain is connected between the first driven chain wheel and the first driving chain wheel. The pig house washing device has the advantages of convenience in operation, time and labor saving, high cleaning efficiency, comprehensiveness in cleaning of grounds and walls, and improved hygiene of pighouses.

A miniature pig running machine belongs to the technical field of animal training or exercise equipment. The invention provides a miniature pig running machine having the advantages of good protective properties and reliability, convenient observation and easy operation. The running machine comprises a supporting framework, wherein the front end of the bottom of the supporting frame is provided with wheels, and the back end is provided with adjusting supporting feet; the front end of the top of the supporting frame is provided with a motor cover and an upright pole, the upright pole is provided with an instrument operation display panel, the back end of the motor cover is provided with a Z-shaped baffle, a lower end horizontal plate of the Z-shaped baffle is pressed under the lower end face of the motor cover, an upper end horizontal plate of the Z-shaped baffle is located above the upper end of the motor cover, and the horizontal size of the Z-shaped baffle is greater than or equal to the horizontal size of the motor cover; and the supporting framework at the back end of the Z-shaped baffle is provided with an internally visual closed body which is provided with the back end having a folding door, the supporting framework at the lower end of the closed body is provided with a running belt in the length direction of the closed body, and the width of the running belt is greater than or equal to the width of the closed body.

The invention discloses a fragrant pig feed and belongs to the technical field of feeds. The fragrant pig feed comprises the following raw materials in parts by weight: 30 to 50 parts of big-leaf fast-growing sophora japonica, 30 to 50 parts of sweet grass, 15 to 20 parts of a vitamin additive, 60 to 80 parts of extruded corn, 30 to 50 parts of fermented soybean meal, 8 to 15 parts of yam flour, 10 to 15 parts of auxiliary material, 60 to 80 parts of water and 20 to 30 parts of complex beneficial microorganism, wherein the auxiliary material is a Chinese traditional herb feed additive. The original flavor and quality of Bama miniature pigs fed by the feed disclosed by the invention are kept. Due to the special effects of the big-leaf fast-growing sophora japonica and the like which comprise a great amount of excellent growth factors, the fragrant pigs are more mellow in meat, are more delicious in taste; lean meat of the fragrant pigs is fine and mellow; fat meat of the fragrant pigs is crispy; soup prepared by the fragrant pigs is thick and white, and the endless aftertaste is achieved; not only is healthy and green meat which is attractive in price and quality and is nuisanceless produced, but also loss of grain feeds is greatly reduced, the cost is reduced and economic benefits are increased.

The invention provides a targeting vector with the swine ApoE gene knocked out as well as a construction method of the targeting vector. The targeting vector takes the carrier pLoxPneo as a skeleton carrier, and comprises a positive screening component, a negative screening marker gene and the 5' homologous arm and 3' homologous arm of the swine ApoE gene. The invention further provides applications of the targeting vector in preparing animal models with the swine ApoE gene knocked out. With the adoption of the method provided by the invention, the healthy homozygote miniature pig with the ApoE gene knocked out is successfully obtained, therefore, a foundation is laid for the development of the novel medicines for treating cardiovascular diseases, and meanwhile, a guarantee is provided for developing pathological study, drug screening, efficacy evaluation and the like.

The invention relates to a preparation method of heterogeneous acellular dermal matrix. The preparation method comprises the following steps: taking bama miniature pig skin as a skin source, reversely taking split-thickness skin by adopting a skin drum, carrying out the steps of removing epidermis and extracting cells, thus obtaining acellular dermis, enabling the acellular dermis to react with carboxymethyl chitosan, thus obtaining acellular dermal matrix, finally, treating the acellular dermal matrix by adopting a softening agent and a humectant, so that the prepared acellular dermal matrix is closer to the natural state of the skin of the human body, and thus the use compliance of the acellular dermal matrix is improved. The heterogeneous acellular dermal matrix prepared by adopting the method disclosed by the invention is low in immunogenicity, meanwhile, the heterogeneous acellular dermal matrix keeps the three-dimensional stereoscopic structure of dermal collagen fibers, improves the stability of the three-dimensional structure of the dermal collagen fibers, and has the excellent biocompatibility.

The invention discloses a Bama miniature pig somatic cell cloning method, which comprises the following steps: 1) preparing related agents; 2) cultivating Bama miniature pig somatic cells; 3) freezing the Bama miniature pig somatic cells; 4) unfreezing the frozen somatic cells; 5) acquiring and externally cultivating pig oocytes; 6) transplanting nucleus; and 7) transplanting embryos. The invention has the advantages of speeding up the research on domestic transgenosis pig technology and human heterogeneous organ transplantation, accelerating the development of animal husbandry in Guangxi even the whole country, and academically filling the gap in China.

The invention relates to a cervical spondylotic myelopathy (CSM) experimental animal model and a making method thereof, belonging to the technical field of osteopathic medicine experimental animal models. According to the technical scheme of the invention, a living rabbit, goat, dog, miniature pig or experimental mouse is used as a model body, a compression nail is placed at C3 cervical vertebra body of the rabbit, goat, dog, miniature pig or experimental mouse, and developing biomaterial is filled inside the filling hole of the compression nail. The making method of the CSM experimental animal model comprises the following steps of: making a hole at the C3 cervical vertebra body, and placing the compression nail into the hole to cause spinal cord ventral compression. The CSM experimental animal model provided by the invention has the following benefits that the C3 cervical vertebra is selected as the vertebral body where the compression nail is placed, which completely breaks the domestic and abroad traditional mode of compression at C4 or C5 cervical vertebra, is a new breakthrough in CSM experimental animal model making and achieves an ideal experimental effect; and the surgical operation is simple and safe, and the change of CSM after long-term chronic compression can be studied in various imaging techniques at the same time.

The present invention discloses an experimental method for evaluating eye irritation by using miniature pig erythrocytes. The method mainly comprises: (1) preparing a suspension of miniature pig erythrocytes; (2) carrying out a miniature pig erythrocyte hemolysis test; (3) carrying out a protein denaturation experiment; (4) predicting a model; (5) carrying out classification and judgment. According to the present invention, the source of the erythrocytes is reliable and safe; the tested material is easy to obtain, and has good uniformity; the test system is simple and cheap, and no special equipment is required; the detection method is rapid, sensitive and universal; the method of the present invention can be directly applicable for safety evaluations of chemicals, cosmetics, pesticides, eye medicines and other substances possibly contacting the eyes, wherein the chemicals comprise surfactants, disinfection products, detergent and the like, the cosmetics comprise shampoo, bath foam, eye cream and the like; the method further can be applicable for rapidly screening, classifying and identifying the eye irritations of the raw materials, the formulas or the products.

The invention discloses an FTO gene for intramuscular fat deposition. The FTO gene is an FTO (fat mass and obesity associated) gene which is separated and cloned from Banna miniature pig muscle tissue and is associated with intramuscular fat deposition. The whole length of the CDA (Complete Coding Sequence) of the gene is 1518bp and the CDS nucleotide sequence is shown as SEQ.ID.NO.1. In addition, the CDS nucleotide sequence codes 505 amino acids of which the amino acid sequence is shown as SEQ.ID.NO.2. Through the detection of a tissue expression profile of the FTO gene in seven tissues, such as pig heart, liver, spleen, lung, kidney, muscle and fat, the result shows that the FTO gene is greatly expressed in the fat tissue and the functions of the FTO gene for regulating and controlling the fat deposition in fat cells are preliminarily shown.

The invention discloses a method for detecting fat deposition of a Bama miniature pig by virtue of the LPL (Lipoprotein Lipase) gene. The method is combined with the research conclusion that the LPL gene is in positive correlation with fat deposition, and fat deposition of the Bama miniature pig is detected based on the expression level of the LPL gene in adipose tissues. The method has the characteristics of being rapid, accurate and convenient to operate and does not influence the normal growth of the pig; the method is suitable for dynamically monitoring fat deposition of a pig in a cultivating process, so that the growing condition of the pig can be mastered at any time, fat excessive deposition is prevented, and thus the optimal carcass lean meat rate of the Bama miniature pig is obtained.

The invention discloses a mobile pig house for Bamaxiang pig breeding, which comprises a pig house body, a railing bar, a breeding plate and a push plate, the pig house body is connected to a drainage pipe through a drainage pipe, and the pig house A non-slip layer is fixed on the house body, a feeding trough is fixed on the fixed vertical bar, the fence rail and the fixed vertical bar are connected to each other, the breeding plate is fixed at the bottom of the pig house body, and the push plate is located at the bottom of the pig house body. The inner side of the cleaning tank, and the outside of the push plate is fixed with a handle, the upper end surface of the push plate is fixed with a partition, the bottom of the pig house body is installed on the universal wheel, and the left and right ends of the pig house body are provided with Outfall. The mobile pig house for Bamaxiang pig breeding uses multiple water spray heads to clean the pig itself, which is more convenient to clean. The leaked feces are processed in a centralized manner, which is more convenient to clean and use.

The invention relates to a breeding method of bama miniature pigs. The breeding method comprises the following steps: piggery is selected, piglets is selected, the miniature pig is fed with infancy feed when the pig is at young age, the miniature pig is fed with adult feed when the pig is grown up, at the same time the pig is continuously fed with water and green vegetables until the miniature pig grows to the weight about 30kg, and then the pigs is crops. The method supplements energy, protein, mineral, vitamins and the like to the miniature pig in the process of breeding, satisfies the need of the rapid growth of the pig, promotes the growth and development both of the miniature pig and improves the healthy state of the pig, improves the immunity of the pig, allows the pig to move in the activity field for 3-5 hours through building activity field, which leads the ratio of lean pork to pork is more than 50%. The method has the advantages that large-scale miniature pig breeding is achieved, the comprehensiveness of gathering food in the wild of the miniature pig is enhanced, disease resistance of the miniature pig is improved, and a feeding method of frankincense pig is provided.

The invention discloses a method for detecting real-time fluorescence quota PCR of epidemic encephalitis B virus and a kit. A primer and a TaqMan probe is designed in accordance with the conserved region of the epidemic encephalitis B virus E gene, which is used for quantitatively detecting nucleic acid copy number of the epidemic encephalitis B virus in samples. Particularly, the forward primer of the primer has a nucleotide sequence of SEQ ID NO: 1 in a sequence table, the reverse primer of the primer has a nucleotide sequence of SEQ ID NO: 2 in the sequence table, and TaqMan probe has a nucleotide sequence in SEQ ID NO: 3 in the sequence table. The invention has larger valuable significance in the field of controlling the quality of biological products related to the human and animal sources and inspecting and quarantining the imported and exported animals, ensures the quality of the related biological products and controls the spread of the epidemic encephalitis B and the medication safety of people. The detection method and the kit of the invention can be used for inspecting the epidemic encephalitis B virus on cerebrospinal fluid samples of the clinical patients, miniature pig brain tissue samples and the biological products, and have wide application prospect.

The inventive subject matter relates to a method for evaluating potential compounds and vaccines for the prevention or treatment of dengue virus infection. The method utilizes pigs as an animal model for the evaluation of test vaccine or drug compounds. The breeds that can be utilized and in the inventive method include Yorkshire or Lancashire as well as miniature pig breeds.

The invention discloses a breeding method for boars of miniature pigs. Healthy miniature piglets which are just born are selected to be fed, an ecological captivity method is adopted, the scatter-feed environment is mainly used, a hog house is used in an auxiliary manner, green feed, energy feed and an auxiliary material which are of different proportions are matched according to the boars, of different ages of days, of the miniature pigs, the feed is abundant in nutrition, the miniature pigs are driven to exercise once at 10 o'clock every morning and are driven to exercise once at 5 o'clock every afternoon, and the exercise time should be kept being 1 h to 2 h. By means of the breeding method, the whole breeding process of the miniature pigs is optimized on the aspects of hog house construction, feed ratios, daily feeding and disease prevention and treatment of breeding, and the breeding environment of the boars of the miniature pigs is overall improved; and by means of the ratios of the feed, for the growth and reproduction characteristics of the miniature pigs, the stress resistance and the disease resistance of the boars of the miniature pigs are improved, and therefore stomachs and intestines are regulated, the anti-stress capacity is increased, the health of the boars of the miniature pigs is improved, the boars are prevented from being fatigue, and the boars, in good health, of the miniature pigs can breed good-quality miniature piglets.

The invention relates to a feeding method of Bama miniature pigs. The feeding method of Bama miniature pigs provided by the invention comprises the following steps that feed formulas of a feed A anda feed B are applied; the feed A and the feed B are mixed according to a proportion of 1:2 and piglets are fed 35-55 days after the piglets are born; the feed A and the feed B are mixed according to aproportion of 1:1 and the piglets are fed 55-80 days after the piglets are born; and the feed A and the feed B are mixed according to a proportion of 2:1 and the piglets are fed 80-120 days after thepiglets are born. The breeding method for breeding Bama miniature pigs provided by the invention has reasonable formulas, is suitable for the growth and development and physiological characteristicsof the Bama miniature pigs, can remarkably increase the adaptability of the change from breast milk to feed of the bred Bama miniature pigs, greatly improves the appetite of the bred Bama miniature pigs, and accelerates the growth of the bred Bama miniature pigs, so that the slaughter time is reduced. The breeding method for breeding Bama miniature pigs is low in cost and accords with the production practice of pig farms.

The invention discloses an easy-to-clean pig food trough for Bamaxiang pigs, which comprises a pig food trough outer shell, a food feed tank and a water tank are arranged inside the pig food tank outer shell, and the inner surfaces of the food feed tank and the water tank are uniform. A leak hole is installed, the upper surface of the food trough is provided with a first slide rail, and the inside of the first slide rail is connected with a slider, and the sliders are connected through an adjustment plate, and the upper end of the adjustment plate is installed with a The first push-pull plate, the right end of the first push-pull plate is connected with a cover plate, the inner and outer sides of the left end of the food trough and the inner and outer sides of the right end of the water tank are respectively provided with nozzles and connecting holes, and the outer shell of the pig food trough The left and right ends of the utility model are all connected with the support base through the support plate, and the pig-calling bell is installed on the outside of the support plate. The easy-to-clean pig trough for Bamaxiang pigs is designed with leak holes, nozzles, pig bells, cover plates, magnets, adjustment plates, grooves and sealing blocks, which can facilitate the cleaning of pig troughs and reduce pig food consumption. Bacteria in the tank.

The invention relates to a processing method of aquatic product, in particular to a processing method of instant leek-flavor sleeve-fish ear. The method adopts fresh or frozen sleeve-fish ear having the greatest outer diameter of less than 5 cm and without pollution or deformity, and comprises the steps of cleaning, middle cutting, scalding, pickling, high-temperature roasting for sterilization, packing, sealing and secondary sterilization. The sleeve-fish ear processed by the steps is of a micro 'pig ear' shape. The method provided by the invention is simple and easy to implement, and has reasonable procedure; the processed sleeve-fish product avoids nutrition loss, has an attractive appearance, and can be eaten when being unpacked, thereby improving convenience in eating sleeve-fish. The soft-package sleeve-fish instant food can be stored for 6 months to one year at normal temperature, and the product is easy to store and circulate.

The invention discloses an intramuscular fat deposition fatty acid transport protein 1 (FATP-1) gene. The gene is the fatty acid transport protein 1 (FATP-1) gene which is separated and cloned from a Bannan miniature pig muscle tissue and is associated with intramuscular fat deposition. The full length of a CDS sequence of the gene is 1,941bp and the CDS nucleotide sequence is shown as SEQ. ID. No. 1. The CDS nucleotide sequence encodes 646 amino acids, and an amino sequence is shown as SEQ. ID. No. 2. The tissue expression spectrums of the FATP-1 gene in seven tissues of a pig, i.e. the heart, the liver, the spleen, the lung, the kidney, the muscle and the fat, are detected, and the result shows that the expression amount of the FATP-1 gene is the highest in fat tissues. The result primarily shows that the FATP-1 gene has a function of regulating and controlling fat deposition in a fat cell.

The invention belongs to the field of animal husbandry breeding, and particularly relates to a breeding method of hybrid Bama miniature pigs. The method is characterized by comprising the following steps of 1 mating and 2 pig manure sewage treating. According to the method, the hybrid Bama miniature pigs are bred by adopting wild pigs in the south as male parents and adopting Bama miniature pigs in Guangxi as female parents, therefore, the disease resistance and the pork quality of the Bama miniature pigs can be improved, the survival rate of the Bama miniature pigs can be increased, and the pig variety can be improved; in addition, pig manure sewage is treated by adopting polyaluminium chloride and crosslinked chitosan microspheres, therefore, the COD value, the pH value, the TN value and the TP value of the sewage are greatly decreased, the water quality is improved, the water utilization rate is increased, pollution of the pig manure sewage to the environment is reduced, and meanwhile the breeding cost is greatly reduced.

The invention discloses a preparation method of a miniature pig with a human complement regulatory protein knocked into a Rosa26 site at a fixed point, which is characterized in that a human complement regulatory protein gene is inserted into a pig ROSA26 gene site in a fixed-point gene recombination mode, so that the miniature pig with constitutive expression of the human complement regulatory protein is constructed. The constructed miniature pig not only avoids the unclear genetic background caused by exogenous random insertion and the unstable expression quantity caused by chromosome separation in the progeny breeding process, but also provides important animal resources for the research of xenotransplantation and immune related diseases in the future, and has important clinical application value.

The invention provides a method for breeding Bama miniature pigs through a fermentation bed. The method for breeding Bama miniature pigs through the fermentation bed comprises the following steps: 1) building a piggery: building the piggery on a sunny slope in a rotatable manner, wherein the piggery faces to the south, ventilating openings are formed around the piggery and in an upper cover, mangers and drinking bowls are arranged at both ends of the piggery, and the fermentation bed is arranged in the middle of the piggery; 2) placing padding on the fermentation bed, covering the padding with a plastic film, and fermenting for 5 to 7 days; 3) when the padding is cooled to 25 DEG C or below, and enabling the Bama miniature pigs to enter the piggery according to the standard that each Bama miniature pig occupies 0.6 to 0.8 m<2> of the padding, and the humidity of the padding is retained at 50 to 55 percent; 4) feeding the Bama miniature pigs with coarse and green feeds respectively in the morning and evening, and slaughtering the pigs after the pigs are raised for 110 to 120 days; 5) performing plowing and harrowing. According to the method for breeding Bama miniature pigs through the fermentation bed, the disease resistance and the growing speed of the Bama miniature pigs are improved through breeding on the fermentation bed, and breeding sanitation is guaranteed; the method is pollution-free.