Pigskin processing method for transdermal experiment

Abstract

The invention discloses a processing method of pigskin for a transdermal experiment. The processing method comprises the following steps: selecting Bama miniature pigs suitable for month age and skin requirements according to customer requirements; cleaning the whole piglet with clean water until no dirt or impurity exists on the skin surface of the piglet; performing anesthesia and bloodletting operation on the piglets, and implementing euthanasia; fixing a piglet, and splitting the skin with a pig killing tool; splitting the piglet; after the pigskin is taken down, subcutaneous fat of the pigskin is removed through a tool, and hair on the surface is removed through a hair removing tool until no hair root protrudes on the skin surface; the processed pigskin is placed on a processing machine, machine scales are adjusted, pigskin processing is started, and the thickness of the pigskin reaches the thickness needed by customers; taking down the pigskin, cleaning, packaging, storing in a cabinet freezer, and detecting and extracting the activity characteristics of the pigskin in a laboratory.

Description

Technical Field

[0001] The invention belongs to the technical field of transdermal experiments, and particularly relates to a pigskin processing method for transdermal experiments. Background Art

[0002] Transdermal testing is a commonly used drug permeability test method. It provides an important basis for drug development and clinical application by evaluating the permeability of drugs on human skin. Since transdermal testing is often used as an important means to evaluate the safety and effectiveness of ointments, creams, gels, semi-solid drugs and cosmetics, many related products need to provide data related to transdermal testing when providing relevant literature information for registration. We usually also use animal, human or artificial ex vivo skin, and cooperate with specific analytical methods to dynamically measure the absorption dose and rate of the drug substance through the skin within a certain period of time, so as to study the permeation behavior of the sample on the skin.

[0003] The results of transdermal tests are affected by many factors. First, the selection of appropriate test skin and its source, processing and storage methods are crucial to the accuracy of the results. Secondly, factors such as the type, pH value and volume of the receiving medium will also affect the test results. In addition, the operating skills and actual operation process of the researchers may also lead to differences in test reproducibility between different laboratories and different operators, making the test results unreliable. Among all the influencing factors, the skin used in the test is undoubtedly the most critical part in determining the results of transdermal absorption tests. Selecting the right skin type, such as human, animal or artificial ex vivo skin, is crucial to simulating the real penetration environment and accurately evaluating the transdermal performance of drugs or cosmetics.

[0004] Therefore, in order to obtain reliable test results, transdermal tests require careful selection of test skin and its source, as well as proper processing and storage. At the same time, appropriate receiving media should be selected according to actual needs. For this purpose, a pig skin processing method for transdermal experiments is proposed. Summary of the invention

[0005] The purpose of the present invention is to overcome the problem in the prior art that in order to obtain reliable test results, the test skin and its source must be carefully selected, and appropriate processing and storage must be performed. A method for processing pig skin for transdermal experiments is provided, which processes Bama Xiang pigs to make the Bama Xiang pig skin meet the requirements of transdermal experiments.

[0006] In order to achieve the above object, the present invention provides a pigskin processing method for transdermal experiment, characterized in that it comprises the following steps: 1) Select Bama Xiang pigs of suitable age and skin requirements according to customer requirements; 2) Wash the whole piglet with clean water until there is no dirt or impurities on the piglet's skin; 3) Anesthetize and bleed the piglets, and euthanize them; 4) Fix the piglet and cut open its skin with a pig-killing tool; 5) Cut open the piglet; 6) After removing the pig skin, use tools to remove the subcutaneous fat of the pig skin, and use hair removal tools to remove the surface hair until there are no hair roots protruding from the skin surface; 7) Place the processed pigskin on the processing machine, adjust the machine scale, and start processing the pigskin to make the thickness of the pigskin reach the thickness required by the customer; 8) Remove the pig skin, clean it, package it, and store it in the freezer; 9) Laboratory testing of the active characteristics of extracted pig skin.

[0007] Preferably, the Bama Xiang pig is 4-6 months old and weighs 2.5-3.5 kg.

[0008] Preferably, the tool is a dermatome.

[0009] Preferably, the skin thickness is 1 mm and the size is 20*30 cm.

[0010] Preferably, the freezing temperature of the freezer is -20°C and the storage time is within 3 months.

[0011] Preferably, the incision site is the abdomen.

[0012] Preferably, the pig skin is taken from one of the back, abdomen and buttocks of the pig.

[0013] The beneficial effects of the present invention are: The present invention uses Bama Xiang pig skin as the transdermal experiment has the following advantages: 1. Transdermal permeability: Bama Xiang pig skin showed good transdermal permeability in transdermal experiments. Its skin tissue structure is similar to that of human skin, and has similar permeability. It can accurately simulate the permeability of human skin and provide reliable data.

[0014] 2. Stability: Bama Xiang pig skin has good stability and can maintain relatively consistent transdermal performance during long-term experiments. This is very important for long-term observation or repeated experiments to ensure the reliability and repeatability of experimental results.

[0015] 3. Anatomical and physiological similarity: Bama Xiang pig skin is similar to humans in terms of anatomy and physiology. Its surface stratum corneum, keratinocyte density and lipid composition are similar to human skin, making transdermal experiments more able to simulate real situations and provide experimental data closer to the human body.

[0016] 4. Availability: Compared with other animal models, Bama Xiang pig has better availability and selectivity. Under the premise of complying with ethical standards, Bama Xiang pig skin samples can be obtained more conveniently for transdermal experiments.

[0017] 5. Economic feasibility: Compared with using human body for transdermal experiments, using Bama Xiang pig skin can reduce experimental costs and ethical considerations. This makes transdermal experiments more economically feasible and provides an effective method in some preliminary studies and screening.

[0018] In summary, the use of Bama Xiang pig skin for transdermal experiments has the advantages of high transdermal permeability, good stability, and high anatomical and physiological similarity. This provides a reliable and economically feasible option for the safety assessment of drugs and cosmetics, and provides researchers with more avenues to explore. DETAILED DESCRIPTION

[0019] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0020] This embodiment provides a pig skin processing method for transdermal experiment, which is characterized by comprising the following steps: 1) Select Bama Xiang pigs of suitable age and skin requirements according to customer requirements; 2) Wash the whole piglet with clean water until there is no dirt or impurities on the piglet's skin; 3) Anesthetize and bleed the piglets, and euthanize them; 4) Fix the piglet and cut open its skin with a pig-killing tool; 5) Cut open the piglet; 6) After removing the pig skin, use tools to remove the subcutaneous fat of the pig skin, and use hair removal tools to remove the surface hair until there are no hair roots protruding from the skin surface; 7) Place the processed pigskin on the processing machine, adjust the machine scale, and start processing the pigskin to make the thickness of the pigskin reach the thickness required by the customer; 8) Remove the pig skin, clean it, package it, and store it in the freezer; 9) Laboratory testing of the active characteristics of extracted pig skin.

[0021] As a technical optimization solution of the present invention, the Bama Xiang pig is 4-6 months old and weighs 2.5-3.5 kilograms.

[0022] As a technical optimization solution of the present invention, the tool is a skin knife.

[0023] As a technical optimization solution of the present invention, the skin thickness is 1 mm and the size is 20*30 cm. Since there is a difference of about 0.2 mm in the thickness of the piglet skin between the abdomen and the back, vertical cutting sampling is adopted.

[0024] As a technical optimization solution of the present invention, the freezing temperature of the freezer is -20°C and the storage time is within 3 months to ensure the experimental effect of the organization.

[0025] As a technical optimization scheme of the present invention, the incision position is the abdomen, and the pig skin is taken from one of the pig's back, abdomen and buttocks, which are relatively thick and have better epidermis and dermis structure, and are closer to the characteristics of human skin.

[0026] The working process of the pig skin processing method for transdermal experiment in this embodiment is as follows: select skin from healthy edible pigs as samples for transdermal experiment, ensure that skin without obvious lesions, damage or infection is selected to ensure the accuracy and reliability of the experimental results. Usually, the back, abdomen and buttocks of the pig are the most commonly used skin collection sites. These sites are relatively thick and have a good epidermis and dermis structure, which are closer to the characteristics of human skin. Fresh skin samples are obtained from slaughtered pigs, and pre-experimental preparations are carried out as soon as possible, the skin is cleaned, the hair is removed, and the subcutaneous tissue is removed to obtain pure skin slices, and finally placed in a freezer and stored in an environment of -20°C. The storage time is within 3 months to ensure the experimental effect of the tissue.

[0027] The above contents are merely examples and explanations of the structure of the present invention. The technicians in this technical field may make various modifications or additions to the specific embodiments described or replace them in a similar manner. As long as they do not deviate from the structure of the invention or exceed the scope defined by the claims, they should all fall within the protection scope of the present invention.

Claims

1. A method for processing pig skin for transdermal experiment, characterized in that: The steps include: 1) Select Bama Xiang pigs of suitable age and skin requirements according to customer requirements; 2) Wash the whole piglet with clean water until there is no dirt or impurities on the piglet's skin; 3) Anesthetize and bleed the piglets, and euthanize them; 4) Fix the piglet and cut open its skin with a pig-killing tool; 5) Cut open the piglet; 6) After removing the pig skin, use tools to remove the subcutaneous fat of the pig skin, and use hair removal tools to remove the surface hair until there are no hair roots protruding from the skin surface; 7) placing the pigskin processed in step (6) on a processing machine, adjusting the machine scale, and starting the pigskin processing so that the thickness of the pigskin reaches the thickness required by the customer; 8) Remove the pig skin, clean it, package it, and store it in the freezer; 9) Laboratory testing of the active characteristics of extracted pig skin.

2. The method for processing pig skin for transdermal experiment according to claim 1, characterized in that: The Bama miniature pigs in step (1) are 4-6 months old and weigh 2.5-3.5 kg.

3. The method for processing pigskin for transdermal experiment according to claim 1, characterized in that: The tool used in step (6) is a skin knife.

4. The method for processing pigskin for transdermal experiment according to claim 1, characterized in that: In step (7), the skin thickness is 1 mm and the size is 20*30 cm.

5. The method for processing pig skin for transdermal experiment according to claim 1, characterized in that: The freezing temperature of the freezer in step (8) is -20°C and the storage time is within 3 months.

6. The method for processing pigskin for transdermal experiment according to claim 1, characterized in that: The incision site in step (5) is the abdomen.

7. The method for processing pigskin for transdermal experiment according to claim 1, characterized in that: The pig skin in step (6) is taken from one of the back, abdomen and buttocks of the pig.