21 results about "Spermatogonium" patented technology

The invention relates to the technical field of biological medicines, in particular to an umbilical cord mesenchymal stem cell exosome and application of the umbilical cord mesenchymal stem cell exosome in preparation of a medicine for treating male infertility caused by hidden testis. The prepared high-concentration umbilical cord mesenchymal stem cell exosome suspension preparation is directly used for treating cultured spermatogonial cells and spermatoblasts in vitro, is injected into the testis of a mouse, and is used for improving the seminiferous tubule and spermatogenic microenvironment of testis tissue and promoting division and proliferation of the spermatogonial cells and the spermatoblasts. The method can effectively reverse oligospermia and azoospermia caused by the hidden testis, and compared with a model without intervention of treatment means, the method can effectively promote increase and even regeneration of sperms in the testis.

A sequential cell culture system and methods for the in vitro generation of human spermatozoa from differentiating spermatogonia (dSPGs) and spermatogonial stem cells (SSCs) is provided. The instant disclosure encompasses the discovery and optimization of distinct, stage-specific culture media and conditions that enable, for the first time, the complete progression of human spermatogenesis outside the body. The process includes: (1) inducing entry of dSPGs and SSCs into meiosis; (2) supporting meiotic progression and completion to round spermatids; and (3) promoting spermiogenesis and the formation of elongated, spermatozoa. The invention further provides compositions, systems, and protocols for each stage, as well as markers and methods for identifying successful progression through each developmental transition. This system enables, for the first time, the complete in vitro recapitulation of human spermatogenesis, providing a platform for research, infertility treatment, and assisted reproductive technologies.

The invention discloses a system for directionally differentiating chicken primordial germ cells into gametes in vitro and a regulation and control method, the system comprises a basic culture medium: based on DMEM / F12, adding 10% of fetal calf serum, 2% of chicken serum, 2 mmol / L of L-glutamine, 1 mmol / L of sodium pyruvate and 5.5 * 10 <-5 > mol / L of beta-mercaptoethanol; the spermatogonium induction culture medium further comprises BMP4, RA and GDNF; the oogonium induction culture medium also comprises BMP15, GDF9 and EGF (Epidermal Growth Factor). According to the system and the regulation and control method, the differentiation efficiency is improved, the culture period is shortened, and the number of cell clusters is remarkably increased.

The invention belongs to the technical field of medicines, and particularly discloses application of afzelin in preparation of an anti-testis aging medicament, and testis aging resistance is particularly improvement or recovery of an aged testis tissue structure. The invention also provides a medicament for resisting testis aging. The medicament contains afzelin. The invention provides a new application of afzelin, the afzelin can be used for saving senescence testicular tissue structure disorder, remarkably reducing the epididymal sperm deformation rate and increasing the number of supporting cells and undifferentiated spermatogonium cells, the new application value of afzelin is developed, and a theoretical basis is provided for developing afzelin into anti-reproductive aging drugs.

The invention relates to the technical field of biomedicine, in particular to a construction method and application of a testis organ capable of differentiating sperms in vitro. The polypeptide hydrogel is used as a three-dimensional scaffold for testicular cell growth, and meanwhile, the testicular cell proliferation promoting liquid and the testicular cell differentiation promoting liquid are added in different culture stages, so that the construction of testicular organs is effectively realized. The obtained testis organs comprise testis seminiferous tubule structures consistent with those in vivo and various testis cells with the same arrangement mode; and in addition, spermatogonium can be successfully induced to differentiate deformed sperms in vitro. The testicular organ construction method provided by the invention is short in period, high in sperm differentiation success rate, simple and convenient to operate and low in cost. The constructed testis organ can be applied to screening of drugs or toxicants, testis ectogenesis models and clinical diagnosis and treatment, and has a wide application prospect and an important scientific value.

The invention provides a composition for promoting proliferation of mesenchymal stem cells and / or relieving spermatogonial cell injury as well as a preparation method and application of the composition, and belongs to the technical field of traditional Chinese medicine compositions. The composition is prepared from the following raw materials in parts by weight: 10-20 parts of ginseng, 5-15 parts of maca, 5-10 parts of oyster peptide, 5-15 parts of sika deer penis, 3-8 parts of sika deer blood, 3-8 parts of fresh ginger, 5-8 parts of herba cistanche, 4-10 parts of sea cucumber peptide, 4-8 parts of rhizoma polygonati, 5-10 parts of burdock root, 2-5 parts of fructus lycii powder and 1-3 parts of folium cortex eucommiae. The composition is prepared into a traditional Chinese medicine preparation by adopting a superfine grinding process, and the traditional Chinese medicine preparation has the effects of promoting proliferation of mesenchymal stem cells (MSCs), repairing spermatogonial cell injury caused by oxidative stress (such as chemotherapy and radiation) or aging (such as age-related), reducing the ROS level, improving spermatogenic dysfunction, assisting in treating male infertility and delaying senescence of a reproductive system and a whole body.

The invention discloses an application of SB216763 in improving the in-vitro proliferation capacity of porcine spermatogonium. Therefore, the SB216763 can be used as an additive of the porcine spermatogonial cell in-vitro culture medium, and the porcine spermatogonial cell in-vitro culture medium combined with various growth factors is prepared. Researches show that clone groups of the porcine spermatogonium cultured by the culture medium additionally added with the SB216763 are increased, the number of the clone groups is remarkably increased, the expression quantity of genes related to proliferation is remarkably increased, apoptosis genes are remarkably reduced, and the expression of genes related to reproductive specificity and pluripotent maintenance is also increased, so that the in-vitro proliferation and long-term maintenance of the porcine spermatogonium are improved, and the porcine spermatogonium can be used for preparing a novel culture medium for the porcine spermatogonium. Therefore, the in-vitro culture level of the PSCs is systematically improved. Therefore, the invention provides a reliable basis for establishing a long-term, stable and efficient in-vitro culture system of the porcine spermatogonium, and provides a stable and efficient cell model for gene editing of the porcine spermatogonium, preparation of transgenic animals and research on reproductive diseases; the method also has potential application value in the aspects of genetic improvement of pig breeds or preservation of male germplasm resources.

A sequential cell culture system and methods for the in vitro generation of human spermatozoa from differentiating spermatogonia (dSPGs) and spermatogonial stem cells (SSCs) is provided. The instant disclosure encompasses the discovery and optimization of distinct, stage-specific culture media and conditions that enable, for the first time, the complete progression of human spermatogenesis outside the body. The process includes: (1) inducing entry of dSPGs and SSCs into meiosis; (2) supporting meiotic progression and completion to round spermatids; and (3) promoting spermiogenesis and the formation of elongated, spermatozoa. The invention further provides compositions, systems, and protocols for each stage, as well as markers and methods for identifying successful progression through each developmental transition. This system enables, for the first time, the complete in vitro recapitulation of human spermatogenesis, providing a platform for research, infertility treatment, and assisted reproductive technologies.

The present disclosure provides bovine animals and methods to create recipient bovine animals for spermatogenic stem cell transplantation / complementation through modulation of the NANOS2 gene. In one embodiment genome editing was used to create bovine animals with insertions or deletions (indels) that inactivate or otherwise modulate NANOS2 gene activity so that resulting male bovines lack functional germ cells yet retain functional testicular somatic cells, and bovine females are fertile. These bovine males can then be transplanted or complemented with donor cells capable of giving rise to cells of the spermatogenic lineage, or spermatogenic stem cells and used for breeding.

The invention belongs to the field of basic medical research, and particularly relates to a testicular toxicity animal model establishing method which comprises the following steps: performing intragastric administration on male rats with a preparation containing podophyllotoxin according to the dosage of 15 mg / kg per day; the medicine is continuously administrated for 4 days; according to the method, the rat can be induced to generate testicular toxicity phenotypes, and the phenotypes at least include that the number of sperms is remarkably reduced, the activity of the sperms is reduced, the testosterone level of serum is abnormally increased, and spermatogenic tubules in testicular tissue are irregular in shape and spermatogenic epithelium is thinned.

The invention relates to the technical field of aquaculture, and discloses a method for manufacturing and transplanting artificial spermatophore of Xinxiu lobsters. The method comprises the following steps: acquiring a physiological and biochemical parameter set, including testis development stage indexes, sperm motility density values and spermatophore matrix component proportions, of the Xinxiu lobsters to be treated; constructing a multi-modal spermatophore quality evaluation link, performing fusion analysis on the multi-source spermatophore feature flow, and determining artificial spermatophore simulation parameters and receptor lobster adaptation parameters; a dynamic environment simulation device is arranged, and a multi-dimensional environment disturbance signal is generated according to the adaptive parameters to simulate transplantation environment fluctuation; establishing a distributed operation framework, and generating a node operation strategy based on a transplantation control node state and environment disturbance; generating a spermatophore transplantation operation instruction based on the simulation parameters; and creating an encrypted anti-interference operation channel, and implementing node cooperative processing and artificial spermatophore transplantation execution. According to the method, a multi-link technology is integrated, and a systematic scheme is provided for making and transplanting the artificial spermatophore of the Xinxiu lobsters.

An object of the present invention is to provide a method for simply and efficiently inducing differentiation of oogonia or prospermatogonia from primordial germ cell-like cells or primordial germ cells. The object is solved by a method for producing oogonia or prospermatogonia comprising a step of culturing primordial germ cell-like cells or primordial germ cells in a medium containing a BMP receptor type 2 signal activator to obtain oogonia or prospermatogonia.

The present application relates to the preparation and application field of biological peptide, and particularly relates to a plectinum peptide and application of the plectinum peptide in preparation of a product for repairing male reproductive system damage function. The present application utilizes green controllable enzymolysis technology to obtain protein polypeptides with nutritional health care and medicinal values, and further researches on the basis to obtain an active peptide sequence with high biological activity, obtains the active peptide with high purity through a chemical synthesis mode, and verifies the biological activity of the peptide by using mouse spermatogonia.

The invention provides a specific marker gene lhh2a. 2.2 of spermatogonium of larimichthys crocea as well as a screening method and application of the specific marker gene lhh2a. 2.2, and belongs to the field of molecular markers and the field of germline stem cell research. According to the invention, the nucleotide sequence of the lhh2a. 2.2 gene is as shown in SEQ ID NO: 1; through screening and analysis of a single cell transcription sequencing technology, and through in-situ hybridization, Vasa gene and PCNA gene co-localization, it is determined that the lhh2a. 2.2 gene can specifically identify spermatogonium in a proliferation state; a core tool and a prerequisite condition can be provided for establishment of a high-efficiency germ cell transplantation technology, purification of spermatogonial stem cells for in-vitro culture and construction of a transgenic pseudosciaena crocea strain.

The invention discloses application of lycium barbarum red in treating male reproductive toxicity, and belongs to the technical field of biological medicine. The invention provides application of lycium barbarum red in preparation of a medicine for treating male reproductive toxicity. The male reproductive toxicity is caused by exposure to benzophenone substances or exposure to diethylstilbestrol. Animal experiments find that the lycium barbarum red can effectively improve testis function damage induced by benzophenone substance BP-3 or estrogen diethylstilbestrol, improve seminiferous tubule damage degree, increase seminiferous epithelium height, increase sperm number, spermatogonium and primary spermatoblasts, improve spermatogenic ability, increase pregnancy rate, improve sperm yield, improve sperm quality and improve sperm quality. The growth and development of offspring are promoted.

The invention discloses a lateolabrax japonicus testis cryopreservation solution which comprises the following components: an MEM culture medium, 35.2% of a buffer solution, 1.3-1.6 M of dimethyl sulfoxide, 0.1-0.3 M of trehalose and 10% of fetal calf serum, and the concentrations are all the concentrations in the MEM culture medium. The invention also discloses an ultralow-temperature cryopreservation method for the testis of the lateolabrax japonicus, which comprises the following steps: adding the testis of the lateolabrax japonicus into the cryopreservation liquid, freezing at-80 DEG C, and transferring to liquid nitrogen for cryopreservation. By adopting the testis cryopreservation liquid provided by the invention, the lateolabrax japonicus testis is subjected to ultralow-temperature cryopreservation, the prepared spermatogonium after recovery still survives, and the survival rate is more than 50%. Reliable donor guarantee is provided for germ cell transplantation, so that the application range of fish germ cell transplantation is remarkably widened.

The invention relates to the field of fertility preservation and protection before adolescence, in particular to a transition culture method before immature testicular tissue transplantation. In a transition culture process before immature testicular tissue transplantation, coexistence states (such as FSH and testosterone continuously exist in a male body) of various growth factors and hormones in an in-vivo microenvironment are simulated, or in-vivo physiological dynamic changes are simulated. According to the transition culture method disclosed by the invention, the huge environmental difference caused by transplanting the testicular tissue before adolescence to the mature testicular tissue is reduced, the proliferation of spermatogonium and the maturation of supporting cells are remarkably promoted, the survival rate of the spermatogonium after transplantation is improved, and a foundation is laid for spermatogenesis after transplantation.

The present invention provides methods for diagnosing or predicting a fertility defect, such as infertility, in a mammalian or human male subject by detecting PPP6R3 or a combination of PPP6R3, EIF3C and EIF4G1. The invention also provides kits and instruments for diagnosing or predicting fertility defects, such as infertility disorders, in mammalian male individuals by detecting said proteins. According to the application, it is found and proved that PPP6R3 is crucial to spermatogenesis for the first time, S39 of EIF3C and S1217 of EIF4G1 are targets for PP6 to regulate spermatogonial differentiation, a new insight is provided for the relation between phosphorylation participated by phosphoprotein phosphatase and spermatogonial differentiation, and deep research on the relation is beneficial to development of a male infertility treatment method.

The application relates to a kit for detecting the differentiation state of fruit fly testis spermatogonia and an application thereof, wherein the kit comprises reagents for detecting the expression level of a Bckdhb gene, the Bckdhb gene being a dominant expression gene in fruit fly testis tissue. By using a UAS / Gal4 system to specifically knock down Bckdhb in spermatogonia and driving the Bckdhb by Bam-Gal4, it is found that the down-regulation of the Bckdhb expression level can cause the spermatogonia differentiation to be blocked, the testis top nucleus deepening distance is increased, the germ cell differentiation is defective, meanwhile, the Bckdhb mRNA level is significantly decreased, and the Piwi mRNA level is significantly increased. The expression level of the Bckdhb can be detected to effectively judge the differentiation state of the spermatogonia. The kit provided by the application can be used for fruit fly testis spermatogonia differentiation research, and has important scientific research application value.

This invention discloses a rapid method for detecting calcium signals in undifferentiated and differentiated spermatogonia. The method uses a mild enzymatic digestion method to prepare a testicular single-cell suspension. Indo-1 AM, Thy-APC, and c-kit-PE are used for staining and labeling. The cell population to be analyzed is determined based on the difference between the lateral and forward scattering areas of the cells in the testicular cell sample. Then, Thy-APC and c-kit-PE are used to label undifferentiated and differentiated spermatogonia, respectively, to further select specific cell groups. Finally, the fluorescence intensity changes of indo-1 AM in each specific cell subpopulation are detected, thereby obtaining the changes in calcium ion concentration in undifferentiated, undifferentiated-to-differentiated, and differentiated spermatogonia. This method can rapidly analyze the calcium ion concentration in undifferentiated, undifferentiated-to-differentiated, and differentiated spermatogonia. The method is simple and convenient, and provides a foundation for identifying spermatogonial dysfunction caused by calcium signal imbalance.

The application discloses a method for efficiently inducing haploid sperms from Chinese Ophiocephalus sertifer primordial cells in vitro, and improves the efficiency of inducing primordial cells to generate functional sperms in vitro, and comprises the following steps: placing the primordial cells in a three-dimensional non-stent type culture material containing a sperm induction culture medium to culture; the sperm induction culture medium is obtained by adding sex hormones, melatonin and ERK1 / 2 activators into a basic sperm culture medium. The application further discloses a culture medium for efficiently inducing haploid sperms from Chinese Ophiocephalus sertifer primordial cells in vitro.