35results about How to "Identification achieved" patented technology

The invention discloses a method for extracting a common traditional Chinese medicine from traditional Chinese medicinal materials and authenticating the common traditional Chinese medicine through MALDI-TOF-MS, and belongs to the technical field of biology. According to the method, the traditional Chinese medicinal materials are smashed at high speed, total protein and polypeptide are extracted from the traditional Chinese medicinal materials, dialysis desalination is conducted, then the MALDI-TOF-MS is adopted for detection, and a traditional Chinese medicinal material protein map is obtained. The simple protein extracting technology and Malditof are combined for authenticating the protein maps of the common traditional Chinese medicinal materials such as astragalus membranaceus, ginseng and liquorice. The method has the advantages of being easy and reliable to implement, and provides selections and references for authenticating the common traditional Chinese medicinal materials.

The invention discloses a method for qualitatively detecting adulteration grease in vegetable oil by utilizing stoichiometry. The method comprises the following steps: acquiring gas chromatographic data, and distinguishing the type of adulterated grease in the vegetable oil by utilizing judgment analysis and clustering analysis. According to the method, thirteen judgment analysis models are established, the steps for comparing with the standard vegetable oil map and comparing the content of fatty acid are omitted when a to-be-detected sample is detected, and the ratio of fatty acid is directly substituted to distinguish the type of the adulterated grease, so that the palm oil, colza oil, cotton seed oil, soybean oil, tea seed oil and peanut oil adulterated in rice bran oil can be distinguished, and the detection accuracy is more than 96 percent. More than 9 percent of adulterated palm oil, more than 2 percent of adulterated colza oil, more than 5 percent of adulterated cotton seed oil and more than 16 percent of soybean oil in the rice bran oil can be distinguished through the clustering analysis of the palm oil, colza oil, cotton seed oil and soybean oil adulterated in rice bran oil.

The invention relates to molecular biological techniques, specifically to a method for identifying new generations breeding prawn varieties. The method comprises the following steps: amplifying and sequencing a mitochondria control zone of colonies to be identified by using primers in the mitochondria control zone; amplifying the haplotype colonies to be identified and according with generations breeding colonies in the sequenced mitochondria control zone by using five pairs of microsatellite sites and performing microsatellite parting to the amplified products; after that, distinguishing and analyzing according to microsatellite parting data to further obtain new generations breeding prawn varieties. The method identifies through molecular markers so as to overcome the difficulty that phenotypic characteristics identify new generations breeding varieties difficultly, finishes detection when prawn individual is small and is not influenced by development stage and size of the prawn individual so as to improve accuracy for identification; the identifying method can identify the new generations breeding prawn varieties.

The invention provides a method and system for identifying a Chinese population epithelial cell body fluid spot tissue source from the gene level. The method comprises the steps of utilizing a first PCR amplification primer set for performing first amplification on body fluid spot DNA, and obtaining a first amplification PCR product, wherein the first PCR amplification primer set is composed of nine pairs of amplification primers corresponding to nine genes of CYP2B7P1, MUC4, L.cri, HTN3, STATH, MUC7, LCE2B, DCD and CCL27 one to one; according to the gene expression condition in the first amplification PCR product, whether or not at least one of vaginal secretion, saliva and skin is contained in body fluid spots is judged. According to the scheme, the Chinese population body fluid tissue source can be effectively determined from the gene level, the step for identifying the body fluid or spot tissue source thereof at a criminal investigation site is greatly simplified, and the accuratescientific basis can be supplied to case character clearing, conviction and sentencing.

The invention discloses a primer pair and a kit for identification or assisted identification of stored bean weevils. The primer pair disclosed by the invention is composed of the following five primer pairs: a primer pair 1 (a sequence 1 and a sequence 2), a primer pair 2 (a sequence 3 and a sequence 4), a primer pair 3 (a sequence 5 and a sequence 6), a primer pair 4 (a sequence 7 and a sequence 8) and a primer pair 5 (a sequence 9 and a sequence 10). Used for identification of species of the stored bean, the kit is not affected by the individual development status of a sample and the integrity of the sample, and has the advantages of being time-saving, high-efficient and accurate; and the primer pair and the kit can be widely applied to the food storage departments and quarantine departments.

The invention discloses a method for identifying plant lactobacillus, which relates to an identifying method. The method solves the problems that the existing method for identifying the plant lactobacillus has long period and high cost. The method comprises the following steps: 1, performing PCR amplification by using a primer containing 22 basic groups; and 2, performing electrophoresis detection so as to realize identification of the plant lactobacillus. The identifying method of the invention has short period, and finishes the identification by only about 2 hours; and compared with the existing identifying method, the identifying method of the invention can identify the plant lactobacillus without special complex instruments and data processing, and has low identifying cost.

The invention discloses a testing device for identifying sealing performance of a nonmetal sealing member and a method thereof. The testing device comprises a device body which is internally providedwith a chamber with an opening in bottom of the device body. The bottom of the device body is provided with a bottom cover which is closely connected with the device body. The chamber is internally provided with a filling member with a gap to the inner wall of the device body. The gap between the inner wall of the device body and the sidewall of the filling member is provided with a to-be-tested nonmetal sealing member. The top of the device body is provided with a liquid injection inlet which is communicated with the chamber. The bottom cover is provided with a detecting hole for detecting whether leakage occurs. The testing device further comprises the components of a plurality of pressurizing pipes for introducing gas, a depressurizing pipe, a heating device, a temperature measuring device and a pressure measuring device. The testing device and the method thereof satisfy a requirement for a device test according to an ISO 10423:2009 appendix F.1.13.5.3 standard and furthermore can be applied to device tests of a petroleum equipment well head and a production tree.

The invention belongs to the technical field of traditional Chinese medicine detection, and particularly provides a method for simultaneously identifying components of rhizoma coptidis and cortex phellodendri, which comprises the following steps: (1) preparing a test solution, a reference solution and a reference medicinal material solution; (2) thin layer chromatography detection: carrying out two-time expansion and respective inspection, wherein butyl acetate-methanol-isopropanol-water is adopted as a developing solvent for first-time developing, n-butanol-water-acetic acid is adopted as a developing solvent for second-time developing, a'secondary thin-layer developing method ' is utilized for the first time, the developing solvent with specific composition is adopted, the same thin-layer plate is adopted, and second-time sample application is not needed; the method can be used for simultaneously and qualitatively identifying the components of rhizoma coptidis and cortex phellodendri, avoids the increase of resources and cost caused by independently and qualitatively identifying the components of rhizoma coptidis and cortex phellodendri, ensures the uniformity, stability and reliability of quality, and has the advantages of high accuracy, time and cost saving and easiness in popularization and application.

A detection kit of group B streptococcus rapid serological typing and a preparation method thereof relate to microbiological detection. The detection kit of group B streptococcus rapid serological typing is provided with a kit body and test paper arranged in the kit body. The test paper is provided with a bottom lining, a sample pad, a marking pad, a coating film, and filter paper, wherein the sample pad, the marking pad, the coating film, and the filter paper are successively connected from the left to the right and are arranged on the bottom lining. The method comprises 1) preprocessing fluorescent particles; 2) activating fluorescent microspheres; 3) combining group B streptococcal antibodies; 4) preparing the marking pad; 5) preparing coating antibodies; and 6) assembling strips. The kit and the method are convenient and fast. When the kit and the method are applied to sample detection, people only needs to wait 10 to 15 minutes after the sample is added and then a detection resultcan be acquired. Operation is simple. The kit and the method can be used for group B streptococcus pure bacteria colonies of clinical culture identification, an enriched culture solution and detection and typing of secretion specimens for clinical routine sampling.

The process of identifying the co-epiphytic dominant bacteria composition and the specific host bacteria of sponge includes the following steps: preparing co-epiphytic microbe macro genome total DNA sample via grinding sponge, treating with lysozyme and proteinase K to release DNA, and phenol-chloroform treatment; PCR amplification with the sample as template and general bacterial primer to obtain 16S rDNA segment smaller than 500 bp; denaturing gradient gel electrophoresis to separate mixed segment and obtain 16S rDNA-DGGE gene fingerprint; recovering DNA bands, secondary PCR amplification, denaturing gradient gel electrophoresis, purification, cloning and sequencing to complete homology and system development analysis; analyzing bands in the DGGE fingerprint to complete the molecular identification of the co-epiphytic dominant bacteria composition; and finding out the differential bands in the DGGE fingerprint to complete the molecular identification of specific host bacteria.

The invention provides a block chain-based data processing and authentication report issuing method and system. The method comprises the steps: obtaining electronic data sent by a user based on an electronic data storage platform; verifying the integrity, compliance and consistency of the electronic data; if the integrity, compliance and consistency of the electronic data pass verification, obtaining corresponding key data from the electronic data according to retrieval keyword information input by judicial expertise personnel, and/or performing skin color processing on skin color inspection materials contained in the electronic data through a skin color percentage algorithm to obtain media information, wherein the media information comprises picture information and video information; storing an authentication record uploaded by the judicial expertise personnel in real time, wherein the authentication record is obtained by the judicial expertise personnel by utilizing the key data and/or the video information to perform judicial expertise; and generating an authentication report based on the authentication record so as to enable a judicial expertise center manager to check the authentication report. According to the invention, judicial expertise can be carried out on electronic data on line.

The invention discloses a DNA (deoxyribonucleic acid) bar code. The nucleotide sequence of the DNA bar code is as shown in SEQ ID NO.1. According to the DNA bar code, an ITS sequence of a muscadine isfirstly disclosed, available molecular markers are provided for species identification of the muscadine, and the DNA bar code further comprises parts of conserved sequences and has universality whenbeing used for species identification of grapes. The invention discloses a method and a primer for preparing the nucleotide sequence of the DNA bar code and can acquire sequences of an ITS area of themuscadine. The invention further discloses a molecular identification method of the muscadine and a detection primer and a kit for identifying the muscadine. PCR (polymerase chain reaction) amplification is performed by the aid of the detection primer or the kit to obtain sequences of ITS areas of the grapes, an amplification sequence and the sequence of the DNA bar code of the muscadine are compared or applied to construction of phylogenetic trees of the grapes, so that grape varieties are accurately identified, and identifying processes are simple to operate, high in efficiency and good inrepeatability.

The invention discloses a method for identifying drought tolerance of a tea tree. The method comprises the following steps of picking young sprouts with leaves; clipping the young sprouts according to a principle that one tree bud is provided with two leaves, spreading and airing the tree buds on a glass dish, putting in a withering room for naturally withering indoors, weighing once every one hour, and taking out when the weight-loss ratio of the tree buds reach to 20 to 50 percent; sticking the withered tree buds on a floating plate, putting the floating plate in water, enabling the lower end of the floating plate to immerse in water and the leaves above water, and rewatering; when the weight gain rate of the tree buds reach to 23 percent to 60 percent, wiping water, putting the rewatered tree buds into a drying oven for drying, and recording the weight after drying; identifying the drought tolerance of the tea tree; utilizing a plant relative water content formula for calculating the relative water content of each tree bud, if the relative water content result is larger than or equal to 57 percent, identifying the tree bud as the tree bud of the tea tree with drought tolerance, and otherwise, identifying the tree bud as the tree bud of the tea tree with drought sensitivity.

The invention relates to the technical field of poultry genetic resources and biology, in particular to a method and primer combination for effectively identifying Luyuan chicken breeds. According to the invention, a group of Luyuan chicken specific genetic loci are obtained by screening and include SNPs loci and INDELs loci. The specific genetic loci are distributed on different chromosomes and are used as identification loci, so that the accuracy and flexibility of a detection test are improved. Secondly, the INDELs loci capable of being effectively used for identifying the Luyuan chicken breeds are obtained by screening for the first time. The identification rate of the genetic specific loci obtained by screening on Luyuan chickens is 80% or above. The identification method provided by the invention can more effectively realize the identification of the Luyuan chickens and is high in practicability; and the method, the primer combination and a kit can be widely popularized and applied, and have important significance for identification and protection of the Luyuan chickens.