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Skin spermatogonium protection liquid and preparation method thereof

A spermatogonia and protection solution technology, applied in the field of skin spermatogonia protection solution and its preparation, can solve the problem of the disappearance of the viability of epidermal stem cells or spermatogonial stem cells, the influence of the viability of epidermal stem cells or spermatogonial stem cells, and the inability of basal medium. Reaching the clinical level and other issues, achieving the effects of stable nature, improved storage time, and reduced shrinkage

Inactive Publication Date: 2017-09-05
CENTURY BIOSTRENGTH BEIJING PTY LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The storage time of direct bottling is generally less than 12 hours, which brings considerable time pressure to the transportation, handover and detection of skin cells or spermatogonia, and also limits the scope of its storage business development; in addition, by adding basic culture base to preserve skin cells or spermatogonial cells, because the basal medium cannot meet the requirements of clinical grades, which brings risks to their preservation and seriously affects the viability of epidermal stem cells or spermatogonial stem cells
In addition, the prior art has not disclosed a stem cell protection solution that can preserve skin cells or spermatogonia at the same time, and a placenta preservation solution of the prior art CN105532641 and its method of use are used to preserve skin cells or spermatogonia. After 12 hours, the viability of epidermal stem cells or spermatogonial stem cells has disappeared; therefore, there is an urgent need to provide a protective solution that can preserve both skin cells and spermatogonial cells

Method used

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  • Skin spermatogonium protection liquid and preparation method thereof
  • Skin spermatogonium protection liquid and preparation method thereof
  • Skin spermatogonium protection liquid and preparation method thereof

Examples

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Embodiment 1

[0030] A kind of skin spermatogonia protection liquid, this protection liquid is that gentamicin is dissolved with DMEM medium aqueous solution, every mL described DMEM medium aqueous solution dissolves described gentamycin 80U, and described DMEM medium aqueous solution The concentration is 13mg / mL.

Embodiment 2

[0032] A kind of skin spermatogonia protection liquid, this protection liquid is that kanamycin is dissolved with DMEM medium aqueous solution, every mL described DMEM medium aqueous solution dissolves 120U of described gentamycin, and described DMEM medium aqueous solution The concentration is 25mg / mL, and the preparation method of this protective solution comprises the steps:

[0033] 1) Take 1 / 3 volume of DMEM medium aqueous solution, add 1 / 4 unit of kanamycin at 10°C, mix well, and let stand for 5 minutes;

[0034] 2) Add 1 / 3 volume of DMEM medium aqueous solution, mix well, add 1 / 3 unit of kanamycin at 10°C, mix well, and let stand for 7 minutes;

[0035] 3) Add the remaining 1 / 3 volume of DMEM medium aqueous solution, mix well, add the remaining 5 / 12 units of kanamycin at 10°C, mix well, and obtain the skin spermatogonial cell protection solution.

Embodiment 3

[0037]A skin spermatogonial cell protection solution, which is formed by dissolving gentamicin, kanamycin and tobramycin with DMEM medium aqueous solution, and dissolving gentamicin in every mL of the DMEM medium aqueous solution 82.5U, kanamycin 15U, tobramycin 18U, the concentration of the DMEM medium aqueous solution is 16.9mg / mL.

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Abstract

The invention provides skin spermatogonium protection liquid and a preparation method thereof. The protection liquid is mainly formed by dissolving aminoglycoside antibiotics in DMEM (Dulbecco's modified eagle medium) culture medium water solution, 80-120U of the aminoglycoside antibiotics are dissolved in every mL of the DMEM culture medium water solution, and the concentration of the DMEM culture medium water solution is 13-25mg / mL. The skin spermatogonial stem cell protection liquid sufficiently simulates a human internal environment and is more suitable for survival of skin stem cells and spermatogonial stem cells and stable in property, the survival rate of the skin stem cells and the spermatogonial stem cells can be remarkably improved, and cultivation of the skin stem cells and the spermatogonial stem cells can be further enlarged while the skin stem cells and the spermatogonial stem cells are preserved.

Description

technical field [0001] The invention belongs to the field of skin spermatogonia preservation, and in particular relates to a skin spermatogonia protection solution and a preparation method thereof. Background technique [0002] Spermatogonia belong to the early developmental stage of male germ cells, which can continuously divide cells, increase the number of cells, and differentiate into spermatocytes. Primitive germ cells are differentiated to form spermatogonia, spermatogonia are replicated to form primary spermatocytes, primary spermatocytes undergo the first meiosis to form secondary spermatocytes, and then undergo the second meiosis to form sperm cells. Spermatogonial stem cells are the most studied at present. [0003] As the largest organ of the human body, the skin has multiple functions such as sensation, temperature regulation, secretion and excretion, and it can also serve as a barrier between the human body and the external environment to maintain the stabilit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/02
Inventor 曹毓琳李霞云白志惠潘新刘世红卢承前
Owner CENTURY BIOSTRENGTH BEIJING PTY LTD
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