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Normal-temperature preservation method for denitrifying bacteria

A denitrifying bacteria, normal temperature technology, applied in the preservation of microorganisms, etc., can solve the problems of uneconomical and applicable, and achieve the effects of economy and simplicity, reduction of storage energy consumption, and simple methods

Active Publication Date: 2018-06-05
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cryopreservation temperature of this method is -20~-70°C, which is not economical and applicable to some bacteria that need short-term preservation.

Method used

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  • Normal-temperature preservation method for denitrifying bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Prepare NO 2 - -N concentration is 200mg / L, glycerol is the carbon source, and the concentration is 2500mg / L in terms of COD. The denitrifying bacteria are cultivated in a 10L biological reaction tank with a temperature of 27°C and a pH of 7.8. The stirring speed is 20 rpm. When the bacteria entered the stable growth period, the culture was stopped, and the bacteria were collected after sedimentation and centrifugation.

[0028] Prepare the preserved nutrient solution, according to the mixture of denitrifying bacteria and preserved nutrient solution, the concentration of nutrients is: the concentration of glycerol is 1.0g / L in terms of COD, and the concentration of NO 2 - -N concentration is 0.2g / L, Fe 2+ The concentration is 0.02g / L, K + The concentration is 0.1g / L, Ca 2+ The concentration is 0.05g / L, Mg 2+ The concentration is 0.1g / L preparation.

[0029] Mix the bacteria with the prepared nutrient solution, add preservation aid A according to the concentratio...

Embodiment 2

[0031] The culture process and operating conditions are the same as in Example 1, except that it is preserved at 0°C. After 12 months, it was taken out to investigate the activity and survival rate of the bacteria. After 10 hours of recovery, the activity of the bacteria can be fully recovered, and the survival rate can reach 95%.

Embodiment 3

[0033] The cultivation process and operating conditions are the same as those in Example 1, except that the preservation aid A is added according to the concentration of the preservative in the system at 1.0 mg / L. After 6 months, it was taken out to investigate the activity and survival rate of the bacteria. After 8 hours of recovery, the activity of the bacteria can be fully recovered, and the survival rate can reach 95%.

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Abstract

The invention relates to a normal-temperature preservation method for denitrifying bacteria. The normal-temperature preservation method comprises the following steps: (1) culturing denitrifying bacteria to a logarithmic phase and collecting the bacteria; (2) preparing a preservation nutrient solution which mainly comprises a carbon source, a nitrogen source, trace substances, etc.; (3) mixing thedenitrifying bacteria obtained in the step (1) with the preservation nutrient solution obtained in the step (2) in a preservation container, and adding a proper amount of a preservation aid, wherein the preservation aid comprises glycolipid, sugar alcohol and organic acid salts; and (4) subjecting mixed liquor obtained in the step (3) to hermetic culture for 4 to 12 h and then carrying out normal-temperature preservation. Compared with the prior art, the method provided by the invention is applicable to normal-temperature long-term preservation of a great amount of denitrifying bacteria and has the advantages of good preservation effect at normal temperature, high survival rate, quick activity recovery, etc.

Description

technical field [0001] The invention relates to a method for preserving bacteria, in particular to a simple and effective method for preserving denitrifying bacteria at room temperature. Background technique [0002] Ammonia nitrogen is a pollutant that the country realizes the total amount of water pollution control. Excessive ammonia nitrogen content in the water body will cause eutrophication of the water quality, resulting in the excessive reproduction of some algae in the water body, and the growth of other organisms will be affected, thus destroying the aquatic ecosystem and causing Water quality deteriorates and affects its function. Biological denitrification technology is currently a hot spot in the research and application of denitrification technology at home and abroad because of its environmental protection, high efficiency and no secondary pollution. Biological denitrification is to first oxidize ammonia nitrogen to nitrate nitrogen or nitrite nitrogen through...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04
CPCC12N1/04
Inventor 孙丹凤高会杰郭志华赵胜楠李宝忠陈明翔
Owner CHINA PETROLEUM & CHEM CORP
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