Preservation method for denitrifying bacteria

Abstract

The invention relates to a preservation method for denitrifying bacteria. The preservation method comprises the following steps: (1) culturing denitrifying bacteria to a logarithmic phase and collecting the bacteria; (2) preparing a preservation nutrient solution for the denitrifying bacteria; (3) mixing the denitrifying bacteria obtained in the step (1) with the preservation nutrient solution obtained in the step (2), and adding a proper amount of a preservation aid, wherein the preservation aid comprises glycolipid, sugar alcohol and organic acid salts; and (4) carrying out low-temperature freezing preservation. Compared with the prior art, the method provided by the invention is applicable to preservation of a great amount of denitrifying bacteria and has the advantages of high survivalrate, quick activity recovery, low energy consumption for preservation and the like.

Description

technical field

[0001] The invention relates to a method for preserving bacteria, in particular to a simple and effective method for freezing and preserving denitrifying bacteria at low temperature. Background technique

[0002] Ammonia nitrogen is a pollutant that the country realizes the total amount of water pollution control. Excessive ammonia nitrogen content in the water body will cause eutrophication of the water quality, resulting in the excessive reproduction of some algae in the water body, and the growth of other organisms will be affected, thus destroying the aquatic ecosystem and causing Water quality deteriorates and affects its function. Biological denitrification technology is currently a hot spot in the research and application of denitrification technology at home and abroad because of its environmental protection, high efficiency and no secondary pollution. Biological denitrification is to first oxidize ammonia nitrogen to nitrate nitrogen or nitrite nitr...

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