Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preservation method for denitrifying bacteria

A technology of denitrifying bacteria and storage aids, applied in the preservation of microorganisms, etc., can solve the problems of high storage costs and high energy consumption for long-term storage, and achieve the effects of high survival rate, economical and convenient storage, and simple methods

Active Publication Date: 2018-06-05
CHINA PETROLEUM & CHEM CORP +1
View PDF7 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method needs to add high-concentration nutrients and preservatives, and the preservation cost is relatively high.
In addition, the cryopreservation temperature is -20~-70°C. In order to improve the preservation effect, usually choose -50~-70°C, and the energy consumption for long-term storage is high.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preservation method for denitrifying bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Prepare NO 2 - -N concentration is 200mg / L, glycerol is the carbon source, and the concentration is 2500mg / L in terms of COD. The denitrifying bacteria are cultivated in a 10L biological reaction tank with a temperature of 27°C and a pH of 7.8. The stirring speed is 20 rpm. When the bacteria entered the stable growth period, the culture was stopped, and the bacteria were collected after sedimentation and centrifugation.

[0029] Prepare the preserved nutrient solution, according to the mixture of denitrifying bacteria and preserved nutrient solution, the concentration of nutrients is: the concentration of glycerol is 1.0g / L in terms of COD, and the concentration of NO 2 - -N concentration is 0.2g / L, Fe 2+ The concentration is 0.02g / L, K + The concentration is 0.1g / L, Ca 2+ The concentration is 0.05g / L, Mg 2+ The concentration is 0.1g / L preparation.

[0030]Mix the bacterium with the prepared nutrient solution, add preservation aid A according to the concentratio...

Embodiment 2

[0032] The culture process and operating conditions are the same as in Example 1, except that the concentration of glycerol is 2.5 g / L in terms of COD. After 2 years, they were taken out to investigate the activity and survival rate of the bacteria. After 2 days of recovery, the activity of the bacteria can be completely restored, and the survival rate can reach 95%. When the amount of protective agent was increased, the preservation effect was basically the same.

Embodiment 3

[0034] The cultivation process and operating conditions are the same as those in Example 1, except that they are stored in a low-temperature refrigerator at -50°C. After 2 years, they were taken out to investigate the activity and survival rate of the bacteria. After 2 days of recovery, the activity of the bacteria can be completely restored, and the survival rate can reach 95%. The preservation effect is basically the same, but the energy consumption of -50 ℃ preservation is relatively high.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a preservation method for denitrifying bacteria. The preservation method comprises the following steps: (1) culturing denitrifying bacteria to a logarithmic phase and collecting the bacteria; (2) preparing a preservation nutrient solution for the denitrifying bacteria; (3) mixing the denitrifying bacteria obtained in the step (1) with the preservation nutrient solution obtained in the step (2), and adding a proper amount of a preservation aid, wherein the preservation aid comprises glycolipid, sugar alcohol and organic acid salts; and (4) carrying out low-temperature freezing preservation. Compared with the prior art, the method provided by the invention is applicable to preservation of a great amount of denitrifying bacteria and has the advantages of high survivalrate, quick activity recovery, low energy consumption for preservation and the like.

Description

technical field [0001] The invention relates to a method for preserving bacteria, in particular to a simple and effective method for freezing and preserving denitrifying bacteria at low temperature. Background technique [0002] Ammonia nitrogen is a pollutant that the country realizes the total amount of water pollution control. Excessive ammonia nitrogen content in the water body will cause eutrophication of the water quality, resulting in the excessive reproduction of some algae in the water body, and the growth of other organisms will be affected, thus destroying the aquatic ecosystem and causing Water quality deteriorates and affects its function. Biological denitrification technology is currently a hot spot in the research and application of denitrification technology at home and abroad because of its environmental protection, high efficiency and no secondary pollution. Biological denitrification is to first oxidize ammonia nitrogen to nitrate nitrogen or nitrite nitr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04
CPCC12N1/04
Inventor 孙丹凤高会杰郭志华赵胜楠李宝忠陈明翔
Owner CHINA PETROLEUM & CHEM CORP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com