Preservation and rejuvenation method for hairy roots of scutellaria baicalensis

A technology of hairy roots and Scutellaria baicalensis, which is applied in the field of preservation and rejuvenation of Scutellaria baicalensis hairy roots, can solve the problems of cumbersome time-consuming, cell degeneration, high cost, etc., and achieve the effects of simple operation, reduced metabolism, and low cost

Active Publication Date: 2014-07-02
奈曼旗国安农业开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Commonly used plant cell preservation methods include liquid nitrogen cryopreservation method and cryogenic refrigeration method: liquid nitrogen preservation requires special equipment and high cost, and is generally only used in professional preservation institutions; laboratories mostly use cryogenic refrigeration to preserve plant cells, but the preservation process The living environment will be changed due to the accumulation of metabolites. Generally, it needs to be retransferred for preservation after 1 to 4 months. Although no special equipment is required, it is cumbersome and time-consuming, so it is limited to short-term preservation, and frequent transplantation can easily cause cell degradation

Method used

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  • Preservation and rejuvenation method for hairy roots of scutellaria baicalensis
  • Preservation and rejuvenation method for hairy roots of scutellaria baicalensis
  • Preservation and rejuvenation method for hairy roots of scutellaria baicalensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for preserving and rejuvenating hairy roots of Scutellaria baicalensis, comprising the following steps:

[0035] (1) Under sterile conditions, select Scutellaria baicalensis Georgi, which has a high hairy root biomass and baicalin content, and pick its light color, straight root system, many branches and slight root surface. For the hairy root tip of Scutellaria baicalensis with obvious villi, the picking site should contain 1 to 5 growth points, and the branch length is 0.2 to 1.0 cm.

[0036] (2) The hairy root of the selected excellent strain was inserted into the liquid medium at an inoculum amount of 0.5%, and the culture condition was 110r·min -1 , 25 ℃ dark culture for 18 days. The pH of the liquid medium is 6.0, containing 30g·L -1 Sucrose, 500mg·L -1 Hydrolyzed milk protein, 2.0mg·L -1 Glycine, 0.5mg·L -1 Thiamine hydrochloride, 0.5mg·L -1 Pyridoxine hydrochloride, 0.5mg·L -1 Niacin and 150mg·L -1 Inositol, and the inorganic salt part.

[0037...

Embodiment 2

[0043] (1) Under sterile conditions, select a strain with high hairy root biomass and baicalin content, and pick the Scutellaria baicalensis hairy root with light color, straight root system, many branches and obvious microvilli on the root surface The tip, the picking part should contain 1 to 5 growth points, and the branch length is 0.2 to 1.0 cm.

[0044] (2) Insert the hairy roots of the selected excellent strains into the liquid medium at an inoculation amount of 0.7%, and the culture condition is 110r·min -1 , 28 ° C dark culture for 20 days. The pH of the liquid medium is 5.8, containing 40g L -1 Sucrose, 500mg·L -1 Hydrolyzed milk protein, 0.5mg·L -1 Glycine, 2.5mg·L -1 Thiamine hydrochloride, 1.5mg·L -1 Pyridoxine hydrochloride, 2.0mg·L -1 Niacin and 50mg·L -1Inositol, and the inorganic salt part.

[0045] (3) Take the hairy root of Scutellaria baicalensis with vigorous vitality and synchronous growth, inoculate it into the preservation medium at an inoculatio...

Embodiment 3

[0051] (1) Under sterile conditions, select a strain with high hairy root biomass and baicalin content, and pick the Scutellaria baicalensis hairy root with light color, straight root system, many branches and obvious microvilli on the root surface The tip, the picking part should contain 1 to 5 growth points, and the branch length is 0.2 to 1.0 cm.

[0052] (2) Insert the hairy roots of the selected excellent strains into the liquid medium at an inoculation amount of 0.8%, and the culture condition is 110r·min -1 , 26 ° C dark culture for 15 days. The pH of the liquid medium is 5.5, containing 50g L -1 Sucrose, 1000mg·L -1 Hydrolyzed milk protein, 4.0mg·L -1 Glycine, 0.5mg·L -1 Thiamine hydrochloride, 0.5mg·L -1 Pyridoxine hydrochloride, 0.2mg·L -1 Niacin and 200mg·L -1 Inositol, and the inorganic salt part.

[0053] (3) Take the hairy root of Scutellaria baicalensis with vigorous vitality and synchronous growth, inoculate it into the preservation medium at an inocula...

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Abstract

The invention discloses a preservation and rejuvenation method for hairy roots of scutellaria baicalensis, and belongs to the technical field of plant cell preservation. The technical scheme of the invention mainly comprises the following steps: chosen root tip parts of the hairy roots of the scutellaria baicalensis are shaded and cultivated in a liquid culture medium to the logarithmic phase; the hairy roots of the scutellaria baicalensis cultivated by the liquid are chosen to add in a preservation culture medium, and are shaded and stewed for preservation at the room temperature; the hairy roots of the scutellaria baicalensis cultivated through preservation are taken to inoculate in an activation culture medium, and are shaded and cultivated for 20-25 days; and the activated hairy roots of the scutellaria baicalensis are taken to inoculate in a rejuvenation culture medium, and are shaded and cultivated for 15-20 days at the temperature of 25-28 DEG C. The preservation and rejuvenation method has low cost, is easy to operate, and solves the problems of high demand on liquid nitrogen preservation speciality, high cost and complexity in conventional refrigeration preservation.

Description

technical field [0001] The invention belongs to the technical field of plant cell preservation, and relates to a method for preserving and rejuvenating hairy roots of Scutellaria baicalensis. Background technique [0002] Plant cell preservation technology is the basis of production and research. Its principle is to artificially put cells into a dormant state, reduce their growth and metabolic activities, and achieve the purpose of inhibiting cell proliferation and reducing variation. Commonly used plant cell preservation methods include liquid nitrogen cryopreservation method and cryogenic refrigeration method: liquid nitrogen preservation requires special equipment and high cost, and is generally only used in professional preservation institutions; laboratories mostly use cryogenic refrigeration to preserve plant cells, but the preservation process The living environment will be changed due to the accumulation of metabolites. Generally, retransplantation and preservation w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 施春阳齐香君钱卫东李雅
Owner 奈曼旗国安农业开发有限公司
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