69results about How to "Inhibition of growth and proliferation" patented technology

The invention discloses a controlled-atmosphere ice-temperature fresh-preservation method for seawater shrimps, which comprises the following steps of: soaking caught seawater shrimps with a 4-hexylresorcinol solution with the mass concentration of 1-2% for 2-4min, wherein the volume of the soaking solution is 1.5-2 times as heavy as the weight of the raw material; disinfecting and sterilizing the soaked seawater shrimps for 5-10min by using ozone water with the water temperature lower than 5 DEG C and the ozone concentration of 1-1.5mg / L, fishing out, rinsing with clean water lower than 5 DEG C, draining, putting into controlled-atmosphere packaging bags or boxes, and injecting a gas mixture of 70-80% CO2 and 20-30% N2 for packaging by using a controlled-atmosphere packaging machine, wherein 2-4mL gas is injected for each gram of seawater shrimps; and storing the packaged product at subzero 2.0 to 0 DEG C. The method can remarkably prolong the shelf life of the seawater shrimps to 8-12 days, ensures the eating safety of the product and maintain the good quality of the product.

The invention discloses a preparation method of a dihydroartemisinin liquid preparation, which belongs to the field of medicine. The preparation method comprises the following steps: firstly, dissolving dihydroartemisinin into dimethyl sulfoxide, then adding ethanol and polyethylene glycol 400 and further diluting the dihydroartemisinin to obtain the dihydroartemisinin liquid preparation which can be mixed and dissolved with water in any proportion. Because of enhancing the dispersion degree of the dihydroartemisinin greatly, the water solubility of the dihydroartemisinin liquid preparation is obviously enhanced. The dihydroartemisinin liquid preparation is used as an anti-tumour preparation and can obviously suppress the growth and the multiplication of the cells of a leukaemia cell strain K562 which is cultured in vitro, so that the anti-tumour effect of the dihydroartemisinin liquid preparation is obviously enhanced.

The invention relates to a combined medicine with a synergistic action for treating T cell lymphomata. The combined medicine comprises bortezomib and SAHA, wherein the proportion of the bortezomib to the SAHA is 5-10nM:1-2.0 mu M. The invention also provides an application of the combined medicine in preparing a medicine for treating lymphomata diseases, wherein the lymphomata is T cell lymphomata. By using the combined medicine, the synergistic action of the two medicines is exerted, and the growth and the multiplication of T lymphomata cells can be synergically suppressed. By combining the two medicines, the curative effect is enhanced, and the cost can also be greatly lowered.

The invention belongs to the technical field of biological medicine, and particularly relates to a method for preparing a protamine-deoxycholic acid conjugate with a heparin transfer function. The amidogen of protamine and the carboxyl of deoxycholic acid form an amide bond through teh effect of a cross-linking agent to prepare an amphipathic conjugate, and then the amphipathic conjugate and heparin form a self-assembled aggregation in order to conveniently release the heparin in cells. The hydrophobic modification of the conjugate can enhance the self-aggregation stability of nano-composites; the cationic performance of the conjugate entraps the heparin, so that the distribution capability of the heparin in the cells is increased, and the heparin is prevented from, degradation caused by the effect of heparinase and further the transfer of the heparin into cancer cells and the release and biological effect of the heparin in the cells are realized.

The invention provides an anticancer traditional Chinese medicine composition, belonging to the technical field of medicines. The traditional Chinese medicine composition comprises bezoar, sweet wormwood herb, musk, root of China creeper and all-grass of nerved twayblade. The traditional Chinese medicine composition has the beneficial effects that through a reasonable ratio of the five medicinal raw materials, the traditional Chinese medicine composition has synergetic effects and the double effects of directly inhibiting cancer cell growth and proliferation and enhancing the immune function; through clinical research trials, the traditional Chinese medicine composition can selectively inhibit and kill cancer cell proliferation to achieve the anticancer effect, can also promote differentiation of T and B lymphocytes and activate NK cells to enhance the body immunity, has quick treatment effects on cancers, does not have toxic or side effect, has a wide anticancer spectrum, especially has obvious curative effects on lung cancers, liver cancers, gastric cancers, intestinal cancers, lymph cancers, brain tumors, nasopharynx cancers, esophagus cancers, pancreatic cancers and cervical cancers, and does not have any impact on body cells, tissues, organ functions and hemogram in the medication period.

The invention relates to a micro / nanometer fiber slow release preparation for treating cicatrices and a preparation method thereof. The micro / nanometer fiber slow release preparation is a complex consisting of panaxoside Rg3 and degradable high molecular polymer fibers, wherein the panaxoside Rg3 is dispersed in the degradable high molecular polymer fibers in a non-crystal form, and accounts for 0.1 to 50 percent of the mass of the complex; and the diameters of the degradable high molecular polymer fibers are between 5 nanometers and 10 micrometers. The method for preparing the micro / nanometer fiber slow release preparation comprises the following steps of: dissolving the panaxoside Rg3 and degradable high molecular polymer in an organic solvent to form mixed solution, spinning the mixed solution in a method of electrostatic spinning, and drying under vacuum to prepare the micro / nanometer fiber slow release preparation. By the micro / nanometer fiber slow release preparation, the release concentration and action time of active medicaments of the panaxoside Rg3 at local parts of the cicatrices are improved, the availability of the medicaments is improved, the long-term release of the medicaments is realized, and the requirement of the panaxoside Rg3 in clinical treatment is met, so the micro / nanometer fiber slow release preparation has the characteristics of high adaptability, simple process, low cost, high repeatability and the like.

The invention belongs to the technical field of food, and particularly relates to a probiotics powder and a preparation method thereof. The probiotics powder of the invention comprises the following components in parts by weight: 8-16 parts of Pu'er tea enzyme powder, 10-18 parts of multiple fungus powder, 6-8 parts of soybean protein isolate powder, 6-12 parts of spirulina powder, 12-20 parts ofmodified yeast glucan, 2-6 parts of black bamboo salt, 4-8 parts of honey and 32-46 parts of fructo-oligosaccharide. The probiotics powder provided by the invention is reasonable and scientific in compatibility, and the components interact with each other. The probiotics powder not only has a good taste and good stability, but also has the effects of moistening intestines, relaxing bowels, expelling toxins, lowering fat and reducing weight.

The invention belongs to the technical field of biological medicines, and particularly relates to a preparation method of a mediated-heparin intracellular delivery crosslinking nanometer carrier. PEG-modified PEI is adopted; and heparin is entrapped by using cation performance of PET, so that the cell endocytosis ability is increased; the water solubility, the stability and the blood metabolic capability of a carrier and nano composites are improved by PEG molecules; removal of combination of composites and protein is avoided; meanwhile, degradation of the heparin caused by heparin enzyme action is avoided; in addition, effective release of the heparin in cells is achieved; and a disulfide bond is introduced through a crosslinking agent on the basis, so that nanoparticles are degraded to release the heparin, thus the heparin plays a biological effect in the cells.

The invention belongs to the technical field of plant tissue culture, and particularly relates to a growth medium capable of promoting seed germination and seedling growth of bletilla striata, a formula of the growth medium and a preparation method. The preparation formula of the growth medium capable of promoting seed germination of bletilla striata comprises the following components: MS or 1 / 2 MS, 0.1-2.0 mg / L of NAA (naphthalene acetic acid), 0.8-4.0 g / L of activated carbon, 40-60 g / L of mashed potatoes, 40-60 g / L of mashed bananas, 1-7 g / L of perilla stem, 2-5 g / L of clematis chinensis, 3-10 g / L of uniflower swisscentaury root, 2-9 g / L of notopterygium incisum, 3-6 g / L of viola mandshurica, 2-5 g / L of lophatherum gracile, 1-4 g / L of vietnamese sophora root, 2-5 g / L of radix asparagi and 4-7 g / L of reed rhizome. The growth medium has the advantages as follows: MS or 1 / 2 MS is taken as the basic growth medium; NAA promotes seed germination; the mashed bananas and the activated carbon facilitate seedling strengthening and rooting; seed germination and seedling growth can be directly promoted by joint application of perilla stem, clematis chinensis, uniflower swisscentaury root and notopterygium incisum; growth of bletilla striata symbiotic bacteria can be promoted by viola mandshurica, lophatherum gracile, the vietnamese sophora root, radix asparagi and reed rhizome, and seed germination and seedling growth are promoted indirectly.

The invention discloses a crop rotation, intercropping and relay intercropping cultivation method for relieving continuous cropping obstacles of konjac. According to the method, a crop rotation, intercropping and relay intercropping cultivation technology is integrated into a konjac planting system, and the konjac and green manure are subjected to crop rotation, so that the land utilization efficiency can be improved, the physical and chemical properties of soil can be improved, the soil fertility can be adjusted, and the continuous cropping diseases of the konjac can be effectively blocked; the konjac and high-stalk crops are intercropped, so that land, water, light and heat resources are fully utilized, and the high-stalk crops provide shading protection for the konjac; the konjac is subjected to relay intercropping to cover plants, and vegetation with proper density and height is rapidly formed on ridges, so that the growth of field weeds is inhibited, the earth surface temperaturein a high-temperature period is reduced, the soil moisture is kept, the diversity of soil organisms is increased, beneficial soil biological communities are promoted, and the growth and proliferationof harmful pathogenic bacteria of soft rot and southern blight are inhibited; and meanwhile, the crop rotation, intercropping and relay intercropping cultivation system interferes with the identification, positioning and damage of pests to the konjac, so that the survival of natural enemies is facilitated, the field pests are inhibited and killed, wounds caused by the pests on stems and leaves ofkonjac plants are reduced, the probability that the konjac is infected by the pathogenic bacteria is reduced, chemical herbicides, bactericides and insecticides do not need to be applied in the growthperiod of the konjac, the green, efficient and sustainable development of the konjac planting industry and the quality safety of konjac products are guaranteed, the konjac industry is greatly promoted, and the comprehensive and rapid development of economy and society in Shaanxi-Sichuan and Yunnan-Guizhou mountainous areas is promoted.

A composite medicine for treating B-cell leucoma is prepared from rituximab and suberoylanilide hydroxamic acid (SAHA). Its advantages are obvious synergistic effect, high curative effect and low cost.

The invention discloses application of CRTAC1 as a target in preparation of a drug for diagnosing and treating lung adenocarcinoma, and the CRTAC1 can be used as a target for growth, proliferation, invasion and migration of lung adenocarcinoma cells. The CRTAC1 has the following functions: the overexpressed CRTAC1 inhibits the proliferation of lung adenocarcinoma cells, subcutaneous tumor formation capacity of nude mice, the migration and invasion of the lung adenocarcinoma cells and the formation of lung metastatic lesions in the nude mice.

The invention discloses a Survivin promoter-controlled suicide gene HSVtk eukaryotic expression vector. The eukaryotic expression vector disclosed by the invention, in combination with an exogenous drug, namely ganciclovir (GCV), has a specific killing effect on KYSE150 esophageal carcinoma cells, and the effect is significantly stronger than that on HepG2 hepatoma carcinoma cells.

The invention discloses tableted candies rich in fucoidin and an application of the tableted candies rich in fucoidin, and relates to the technical field of foods. The tableted candies comprise the following raw materials in parts by weight of 0.1-30 parts of fucoidin, 0.1-5 parts of chitosan oligosaccharide, 5-10 parts of sodium alginate, 30-60 parts of materia medica stomach nourishing powder, 5-30 parts of sorbitol, 1-10 parts of microlite cellulose, and 0.5-1 part of magnesium stearate, wherein the materia medica stomach nourishing powder is a composition of any one or more of hedgehog mushroom powder, ginger powder, poria cocos powder, dried orange peel powder, broccoli powder, haw powder and Chinese jujube powder, and the specification of the obtained tableted candies is 0.2g / tablet-2.0g / tablet. The fucoidin, the chitosan oligosaccharide, the sodium alginate and the materia medica stomach nourishing powder are used as main raw materials, and are medicinal and edible or common food raw materials, so that the tableted candies are leisure foods which can effectively eliminate helicobacter pylori in oral cavity and stomach, invigorate the spleen and nourish the stomach, and can prevent side effects on human bodies caused by antibiotics for conventional medication.

The invention relates to an anticancer active oligopeptide and an expression vector and application thereof, in particular to anticancer active OPB (oligopeptidase B) oligopeptide and an expression vector and application thereof. A sequence of the oligopeptide is Gly-Asn-Lys-Lys-Trp-Glu-Gln-Lys-Gln-Val-Gln-Ile-Lys-Thr-Leu-Glu-Gly-Glu-Phe-Ser-Val-Thr-Met-Trp-Ser-Ser. The oligopeptide is used to prepare anticancer drugs. The expression vector pMBP-2xOPB is constructed. A peptide fragment synthesized by using the OPB sequence can inhibit the growth and proliferation of tumor cells, and it is shown that the OPB-based peptide fragment and its derivatives are potential drugs for treating cancers.

The invention discloses application of carboline compounds in preparation of drugs for resisting human tongue squamous cell carcinoma. According to the application, discovered through researches, on one hand, the carboline compounds A and B can inhibit growth and propagation of a human tongue squamous cell carcinoma cell CAL-27, and on the other hand, activity of migration of the human tongue squamous cell carcinoma cell CAL-27 can be inhibited, so that the carboline compounds A and B can be developed into antitumor drugs for inhibiting growth and / or transferring of the human tongue squamous cell carcinoma.

The invention belongs to the technical field of gene diagnosis and treatment, and discloses an INPP5B gene and application of an expression product of the INPP5B gene in diagnosis and treatment of breast cancer. The INPP5B gene is used for preparing products for diagnosing and treating the breast cancer. The INPP5B gene and the protein expression product thereof gene can be used as specific markers for diagnosing the breast cancer. The INPP5B gene and the expression product thereof can also be used as a gene treatment tool for preparing the product for treating the breast cancer, thereby providing a new breast cancer treatment approach.

In order to solve the problems existing in preparation of a metal material super-hydrophobic film in the prior art, the invention provides a micro-nano super-hydrophobic film on a metal substrate surface, a preparation method and application thereof. The preparation method comprises the following step (1) of taking pure water with the resistivity of 1.0 [mu]S / cm-50.0 [mu]S / cm as solvents, and dispersing fatty acid salts or detergents containing the fatty acid salts in the pure water for forming uniform aqueous solutions or emulsion, wherein the detergents containing the fatty acid salts comprise different kinds of toilet soap, washing soap, laundry powder, detergents, liquid laundry detergents and hand lotion containing the above components; and the step (2) of soaking cleaned metal materials in the aqueous solutions or the emulsion obtained in the step (1) at 0-60 DEG C, so that a super-hydrophobic film is formed on the surface of the metal substrate. The super-hydrophobic film prepared with the preparation method is of stripy, irregular flaky and acicular micro-nano structures, and the static contact angle with water is larger than 150 DEG.

The invention relates to the technical field of medicine application, in particular to application of radix bupleuri liver-soothing powder to preparation of a medicine for non-surgical or chemoradiotherapy treatment of breast cancer. Studies discover that radix bupleuri liver-soothing powder can inhibit growth and proliferation, as well as clone formation ability, of the breast cancer cells and growth of breast cancer transplantation tumors. According to the complicated etiology and pathology of breast cancer, the medicine is mainly used for dispersing the liver and rectifying qi, and promoting blood circulation to remove blood stasis, and can achieve the treatment effect through overall body conditioning. The medicine can be directly used for treating breast cancer without surgeries or chemoradiotherapy to achieve a very good anti-tumor effect, and therefore, a simple and effective treatment medicine is provided for treatment of breast cancer. Meanwhile, the medicine is a traditional Chinese medicine compound, can be widely used clinically, is small in side effect and definite in curative effect, so that the life quality of patients is improved, and the economical burden of the patients is relieved.

The invention relates to Bombinin-8, a gene of the Bombinin-8 and application of the Bombinin-8 in pharmaceuticals. The Bombinin-8 provided by the invention has broad-spectrum antibacterial activity, plays a role in inhibiting the growth and propagation of three kinds of liver cancer cells and can be applied to the preparation of antitumor pathogenic microorganism infection resisting drugs.

The invention discloses a bacterial RNase E truncation body and application thereof. A protein sequence of the bacterial RNase E truncation body is shown in SEQ ID NO.1. An N end of the RNase E is used for degrading a functional domain of RNA, and a nucleotide coding sequence of the truncation body (Ret) is synthesized in vitro, an SV40 nuclear localization sequence is added at a 5' end of the RNase E, so that the RNase E is expressed in a cancer cell nucleus, various RNAs including mRNA are directly degraded in the cell nucleus, and the biosynthesis of oncoprotein is subjected to interference, thereby inhibiting the growth, proliferation, migration and invasion of cancer cells, and being expected to achieve the effect of inhibiting tumors.

The invention discloses a preparation method for an antibacterial and adsorbing mycotoxin nano-montmorillonite feed additive, and belongs to the field of livestock and poultry feed additives. The additive is formed by the following raw materials, by weight percentage: 40-50% of nano-montmorillonite, 1-5% of zinc oxide, 4-6% of yeast cell walls, 5-8% of bacillus subtilis freeze-dried powder, 4-5% of vitamin C, 10-15% of propionic acid / propionate, and 7-11% of sodium diacetate. The additive has strong ion exchange capacity and mycotoxin adsorption capacity, and the loss of nutrients cannot be produced; and the additive can effectively prevent diarrhoea in livestock and poultry, inhibit the propagation of mould, adsorb and degrade mycotoxins, mitigate the influence of the mycotoxins on the growth of livestock and poultry, improve intestinal flora balance in the livestock and poultry, enhance the growth performance of the livestock and poultry, and effectively reduce the use of antibioticsduring livestock and poultry breeding.

The invention relates to a fermentative composition with a cervical cancer resisting function. The fermentative composition is prepared by fermenting a culture medium; the culture medium is prepared from the following components in percentage by weight: 2 to 4 percent of cortex phellodendri, 1 to 3 percent of rhizoma atractylodis, 1.5 to 2.5 percent of fructus kochiae, 1 to 2 percent of rhizoma curcumae, 2 to 3 percent of herba cirsiijaponici, 1.5 to 2.5 percent of radix salviae miltiorrhizae, 0.8 to 1.5 percent of scolopendra, 1 to 2 percent of rhizoma coptidis, 0.5 to 1 percent of rhizoma cyperi, 0.5 to 1 percent of fructus cnidii, 0.5 to 1 percent of fructus perillae, 0.2 to 0.5 percent of scorpion, 0.5 to 1 percent of excrementum pteropi, 0.2 to 2 percent of radix angelicae sinensis, 0.5 to 1 percent of radix dipsaci, 6 to 10 percent of carbon source, 1 to 5 percent of nitrogen source, 2 to 10 percent of fermentation strain, and the balance of water. The invention also provides a preparation method of the fermentative composition. The fermentative composition provided by the invention has a better inhibiting effect on cervical cancer cells, has no side effect on a human body, and is simple in preparation process.

The invention belongs to the technical field of medicine, and relates to a biologically safe antitumor drug sensitizer, and a preparation method and application thereof. The fluorescent carbon quantum dot is prepared by taking sugar as a raw material and oil as a reaction solvent through heating and stirring similar to traditional cooking. Compared with a traditional carbon quantum dot preparation method, the preparation method is simple, time-saving, easy to implement, simple in preparation condition requirement, low in cost and capable of achieving large-scale preparation. The prepared fluorescent carbon quantum dot is uniform in size, stable and adjustable in fluorescence emission, high in water dispersibility, high in safety and good in biocompatibility. Meanwhile, the chemical structural characteristics of the carbon quantum dot are similar to those of glucose, the carbon quantum dot can competitively inhibit glucose uptake of tumor cells, interfere growth and proliferation of the tumor cells, improve the sensitivity of the tumor cells to various antitumor agents and improve the tumor treatment effect, and the carbon quantum dot is a sensitizer for widely enhancing the tumor treatment effect, and can be popularized and applied to combined treatment of various tumors.

The invention provides application of cephaeline hydrochloride in preparing medicine for treating glioma, and belongs to the technical field of medicine. The method comprise the steps that an MTT method is adopted for detecting the influence of the cephaeline hydrochloride on growth and proliferation of human glioma cell lines U251 and SHG44; genomic DNA electrophoresis, DAPI staining, TUNEL staining and other methods are adopted for detecting the apoptosis induction effect of the cephaeline hydrochloride on human glioma cell SHG44 cells cultured in vitro. The results show that the cephaelinehydrochloride has the effect of inhibiting proliferation of the glioma cells.

The invention discloses a preparation method for tumor suppressor gene-modified mesenchymal stem cells. The method comprises the following steps: A) separation of MSCs and primary culture and subculture; B) building of a tumor suppressor gene recombinant vector; and C) expression and identification of the tumor suppressor gene in MSCs. According to the method, recombination of a target tumor suppressor gene is carried out by adopting an adenovirus vector, the target gene is jointly expressed in the MSCs after the recombinant plasmid transfects the MSCs, and the preparation method has high infection rate and coexpression efficiency. Compared with other virus vectors, the vector disclosed by the invention has the advantages that the adenovirus transduction efficiency is relatively high, and fissional stem cells can be efficiently transfected, and the vector is not integrated into chromosome. Therefore, the safety is relatively high.

The invention belongs to the technical field of tissue culture of traditional Chinese medicine plants, and particularly relates to a pleionebulbocodioides rolfe seed germination culture medium formulaand a preparation method. The pleionebulbocodioides rolfe seed germination culture medium formula is prepared from 1 / 2 MS or 1 / 4 MS, 0.5-2.5 mg / L of 6-BA, 0.1-0.3 mg / L of NAA, 0.1-0.25 g / L of GA, 40-50 g / L of a mashed banana, 3-15 g / L of a rosa roxburghii juice, 2-15 g / L of pueraria lobata, 1-15 g / L of a hawthorn, 2-10g / L of umbellate pore fungus, 1-10g / L of polygonum capitatum and 1-10g / Lof lonicera confusa. The formula has the advantages that the rosa roxburghii juice is used as one of preparation materials of the culture medium, other traditional Chinese medicine components are added on the basis, which can promote seed germination, shorten the germination time of pleionebulbocodioides rolfe seeds and increase the seed germination rate, and the formula can inhibit the growth ofhybrid bacterium on the culture medium compared with a conventional culture medium.

The invention discloses an application of a 2-(2,2,2-trifluoroethylidene)-1,3-diketone compound in preparation of anti-lung cancer drugs, and relates to an application in the field of medicines. The compound is preferably 1,3-bis(4-(tert-butyl) phenyl)-2-(2,2,2-trifluoroethylidene) propane-1,3-diketone, can inhibit the growth and proliferation of a human lung cancer cell line, is combined with a TrxR1 protein specific region of human lung cancer cells, inhibits the functional activity of the human lung cancer cells, up-regulates the ROS level of the cells, finally blocks the cycle of the humanlung cancer cells to a G1 stage or a G2 / M stage, promotes apoptosis or non-apoptotic death of the human lung cancer cells, and is a potential new anti-lung cancer drug.

The invention discloses siRNA for targeted inhibition of Mus81 gene expression, siRNA plasmid, interfering lentivirus and a construction method and application thereof; a siRNA sequence of human Mus81 gene is designed, the corresponding siRNA plasmid and interfering lentivirus are constructed, lentivirus mediation siRNA method is used for specific inhibition of Mus81 gene expression in hepatoma carcinoma cells so as to realize the effects of inhibition of hepatoma carcinoma cell growth proliferation, promotion of hepatoma carcinoma cell apoptosis and improvement of hepatoma carcinoma cell chemosensitivity, and other various aspects, the Mus81 gene which is a very effective drug target for treatment of liver cancer is provided, and the siRNA has great significance to preparation of Mus81 gene-targeted treatment drugs of the liver cancer.