Target CRTAC1 for inhibiting lung adenocarcinoma and application of target CRTAC1

A 1. CRTAC1, lung adenocarcinoma technology, applied in the biological field

Active Publication Date: 2020-10-20
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current research on the CRTAC1 gene mainly focuses on genomics analysis, but in terms of basic medicine

Method used

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  • Target CRTAC1 for inhibiting lung adenocarcinoma and application of target CRTAC1
  • Target CRTAC1 for inhibiting lung adenocarcinoma and application of target CRTAC1
  • Target CRTAC1 for inhibiting lung adenocarcinoma and application of target CRTAC1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1. The expression of CRTAC1 is uniformly down-regulated in lung adenocarcinoma tissues and cell lines

[0065] 1) The expression of CRTAC1 mRNA in 58 pairs of lung adenocarcinoma patients in the TCGA database was analyzed by t-test using GraphPad Prism software. Pfigure 1 A).

[0066] 2) Tissue samples

[0067] The clinical samples of lung adenocarcinoma used in the present invention were provided by a cooperative hospital, and the tissue was diagnosed as lung adenocarcinoma by a pathological physician. During specimen collection, a position about 3 cm around the lung adenocarcinoma tissue was selected as the paracancerous tissue control. A portion of the tissue was fixed with 4% PFA, and the rest was frozen at -80°C for later use. All patients have detailed information such as name and pathological information. The experimental procedure was approved by ethical review.

[0068] 3) Tissue / cell total RNA extraction

[0069] 1. Take out the collected lung canc...

Embodiment 2

[0098] Kaplan-Meier survival analysis was performed on the survival information of CRTAC1 in 401 lung adenocarcinoma patients in the TCGA database using GraphPad Prism software. The high expression of CRTAC1 (>median; n=109) and the low expression of CRTAC1 ( figure 2 A). That is, the expression of CRTAC1 was significantly positively correlated with the survival of lung adenocarcinoma patients ( figure 2 A).

Embodiment 3

[0099] Example 3. Overexpression of CRTAC1 significantly inhibits the proliferation of lung adenocarcinoma cells

[0100] 1) Select H1299 and A549 cells as research objects, infect H1299 and A549 cells with GFP-CRTAC1 plasmid and GFP empty plasmid virus supernatant, and use Puromycin drug screening to establish stable cell lines H1299 (CRTAC1), A549 (CRTAC1) and Its control cell lines H1299 (Vector), A549 (Vector). The overexpression effect was detected by Western Blot, and β-Actin was used as an internal reference, such as image 3 A and 3D are shown.

[0101] 2) The soft agar colony formation experiment was used. At room temperature, a medium was prepared in a clean bench, and 12.6ml (1.8ml×7) was preheated at 45-46°C for more than 10min. Microwave heating and boiling agar for 3 times, after cooling to 45-46°C, take 8.4ml (1.2ml×7) of agar and mix quickly in a medium preheating tube, absorb 3ml / well and spread the next layer of agar glue, and let it solidify naturally for mo...

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PUM

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Abstract

The invention discloses application of CRTAC1 as a target in preparation of a drug for diagnosing and treating lung adenocarcinoma, and the CRTAC1 can be used as a target for growth, proliferation, invasion and migration of lung adenocarcinoma cells. The CRTAC1 has the following functions: the overexpressed CRTAC1 inhibits the proliferation of lung adenocarcinoma cells, subcutaneous tumor formation capacity of nude mice, the migration and invasion of the lung adenocarcinoma cells and the formation of lung metastatic lesions in the nude mice.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a new target for the research on growth, proliferation and metastasis of lung adenocarcinoma cells and its application, detecting the mRNA or protein expression level of CRTAC1, which can further be used as a diagnostic and prognostic marker for lung adenocarcinoma, and expectations It has become a new target for the treatment of lung adenocarcinoma. Background technique [0002] Lung cancer is one of the malignant tumors with the fastest growing morbidity and mortality, and the greatest threat to the health and life of the population. The five-year survival rate of lung cancer patients in most countries is between 10-19%, and in China it is 19.4-20.2%. According to the latest statistics from my country Cancer Center, lung cancer ranks first in the country, with an annual incidence of about 781,000, accounting for 20.55% of all types of cancer. Lung cancer is the cancer ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886G01N33/574A61K45/00A61P35/00
CPCA61K45/00A61P35/00C12Q1/6886C12Q2600/158G01N33/57423G01N33/57484
Inventor 黄海山常园园李海英金红蕾
Owner WENZHOU MEDICAL UNIV
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