Pleionebulbocodioides rolfe seed germination culture medium formula and preparation method

A technology of germination medium and medium formulation, which is applied in the field of ice ball seed germination medium formulation and preparation, can solve the problems of small reproduction coefficient, low seed germination rate, and shorten seed germination time, so as to promote germination and growth, inhibit The effect of growth and proliferation, preventing the growth of miscellaneous bacteria

Pending Publication Date: 2019-05-31
毕节市中药研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Although the above-mentioned scheme solves the deficiencies of the prior art to a certain extent, in the disclosed patent, the pseudobulb of the ice ball is used as the propagation material, and compared with the seed propagation, its propagation coefficient is small and the growth rate is slow; in Liao Tingting et al. In the study, the growth medium of the tissue bud block explants could not jointly promote the seed germination and seedling growth of the ice ball, and

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  • Pleionebulbocodioides rolfe seed germination culture medium formula and preparation method
  • Pleionebulbocodioides rolfe seed germination culture medium formula and preparation method
  • Pleionebulbocodioides rolfe seed germination culture medium formula and preparation method

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Experimental program
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Example Embodiment

[0020] Example 1

[0021] A preparation formula of ice hockey seed germination medium includes the following components: 1 / 2MS or 1 / 4MS, 0.5~2.5mg / L 6-BA, 0.1~0.3mg / L NAA, 0.1~0.25g / L GA, 40-50g / L banana mash, 3-15g / L prickly pear juice, 2-15g / L kudzu root, 1-15g / L hawthorn, 2-10g / L polyporus, 1 ~10g / L Polygonum capitatum, 1~10g / L Lonicera, 6~10g / L carrageenan, 25~30g / L white granulated sugar.

[0022] 1 / 2MS or 1 / 4MS is used as a basic medium, 6-BA, NAA and GA promote seed germination, banana puree and prickly pear juice are conducive to the rooting of prickly pear seedlings. Pueraria lobata, Hawthorn, Polyporus umbellatus, Polygonum capitatum and Lonicera japonica are components of Chinese medicine, which can promote the germination and growth of seeds, effectively inhibit the growth and proliferation of bacteria on the medium, avoid the growth of the medium, and help improve the speed of ice balls The efficiency of reproduction.

[0023] The method of the present invention for ...

Example Embodiment

[0033] Example 2

[0034] A preparation formula of ice ball seed germination medium includes the following components: 1 / 2MS, 1.0mg / L 6-BA, 0.2mg / L NAA, 0.15g / L GA, 45g / L banana puree , 10g / L prickly pear juice, 5g / L kudzu root, 4g / L hawthorn, 4g / L polyporus, 4g / L Polygonum capitatum, 2g / L pineapple, 8g / L carrageenan, 30g / L white granulated sugar.

[0035] Control experiment 2: A hockey puck seed germination medium prepared with the preparation formula of this example was used as the experimental tissue culture medium; the other components were: 1 / 4MS+0.1mg / L NAA+2.0mg / L 6- BA + 3% sucrose + 0.5% activated carbon + 0.55% agar was used as the control medium. The experiment operation is as follows:

[0036] After washing the fruit with running water, first disinfect and sterilize with 0.1% mercury liter solution for 8 minutes, then disinfect with 75% alcohol for 4 minutes, and rinse with sterile water for 5 to 8 times. Under aseptic conditions, cut the fruit and gently shake the po...

Example Embodiment

[0040] Example 3

[0041] A preparation formula of ice ball seed germination medium includes the following components: 1 / 2MS, 2.0mg / L 6-BA, 0.1mg / L NAA, 0.1g / L GA, 40g / L banana puree , 10g / L prickly pear juice, 5g / L kudzu root, 4g / L hawthorn, 4g / L polyporus, 4g / L Polygonum capitatum, 2g / L pineapple, 8g / L carrageenan, 30g / L white granulated sugar.

[0042] Control experiment 2: A hockey puck seed germination medium prepared with the preparation formula of this example was used as the experimental tissue culture medium; the other components were: 1 / 2MS+1.5mg / l IAA+0.05% casein+2 % Sucrose + 0.75% activated carbon + 0.55% agar was used as the control medium. The experiment operation is as follows:

[0043] After washing the fruit with running water, first disinfect and sterilize with 0.1% mercury liter solution for 8 minutes, then disinfect with 75% alcohol for 4 minutes, and rinse with sterile water for 5 to 8 times. Under aseptic conditions, cut the fruit and gently shake the powd...

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Abstract

The invention belongs to the technical field of tissue culture of traditional Chinese medicine plants, and particularly relates to a pleionebulbocodioides rolfe seed germination culture medium formulaand a preparation method. The pleionebulbocodioides rolfe seed germination culture medium formula is prepared from 1/2 MS or 1/4 MS, 0.5-2.5 mg/L of 6-BA, 0.1-0.3 mg/L of NAA, 0.1-0.25 g/L of GA, 40-50 g/L of a mashed banana, 3-15 g/L of a rosa roxburghii juice, 2-15 g/L of pueraria lobata, 1-15 g/L of a hawthorn, 2-10g / L of umbellate pore fungus, 1-10g / L of polygonum capitatum and 1-10g / Lof lonicera confusa. The formula has the advantages that the rosa roxburghii juice is used as one of preparation materials of the culture medium, other traditional Chinese medicine components are added on the basis, which can promote seed germination, shorten the germination time of pleionebulbocodioides rolfe seeds and increase the seed germination rate, and the formula can inhibit the growth ofhybrid bacterium on the culture medium compared with a conventional culture medium.

Description

technical field [0001] The invention relates to the technical field of ice ball seed tissue culture, in particular to a formulation of a seed germination medium for ice ball seeds and a preparation method. Background technique [0002] puck ( Oreorchis patens (Lindl.) Lindl.) also known as Shan Arrowhead, Mao Arrowhead, and Nibinzi are the pseudobulbs of the perennial orchidaceae herbaceous plant Suanlan, which has the effects of reducing swelling, dispelling stagnation, reducing phlegm, and detoxification, and is widely used in anti-tumor Among the drugs. In particular, the huge medicinal value is in short supply in the market. [0003] The seeds of ice balls are small, the pollination rate is low under natural conditions, the seeds have no endosperm, they cannot provide the nutrients required for germination, and rarely germinate into seedlings. Due to over-exploitation in recent years, the resources of wild ice hockey are scarce, and with the deterioration of environm...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 查钦阮培均张翔宇王永邹涛柳敏周茂嫦李恒谦黄晓旭王彩云成忠均
Owner 毕节市中药研究所
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