Pleionebulbocodioides rolfe seed germination culture medium formula and preparation method

Abstract

The invention belongs to the technical field of tissue culture of traditional Chinese medicine plants, and particularly relates to a pleionebulbocodioides rolfe seed germination culture medium formulaand a preparation method. The pleionebulbocodioides rolfe seed germination culture medium formula is prepared from 1 / 2 MS or 1 / 4 MS, 0.5-2.5 mg / L of 6-BA, 0.1-0.3 mg / L of NAA, 0.1-0.25 g / L of GA, 40-50 g / L of a mashed banana, 3-15 g / L of a rosa roxburghii juice, 2-15 g / L of pueraria lobata, 1-15 g / L of a hawthorn, 2-10g / L of umbellate pore fungus, 1-10g / L of polygonum capitatum and 1-10g / Lof lonicera confusa. The formula has the advantages that the rosa roxburghii juice is used as one of preparation materials of the culture medium, other traditional Chinese medicine components are added on the basis, which can promote seed germination, shorten the germination time of pleionebulbocodioides rolfe seeds and increase the seed germination rate, and the formula can inhibit the growth ofhybrid bacterium on the culture medium compared with a conventional culture medium.

Description

technical field [0001] The invention relates to the technical field of ice ball seed tissue culture, in particular to a formulation of a seed germination medium for ice ball seeds and a preparation method. Background technique [0002] puck ( Oreorchis patens (Lindl.) Lindl.) also known as Shan Arrowhead, Mao Arrowhead, and Nibinzi are the pseudobulbs of the perennial orchidaceae herbaceous plant Suanlan, which has the effects of reducing swelling, dispelling stagnation, reducing phlegm, and detoxification, and is widely used in anti-tumor Among the drugs. In particular, the huge medicinal value is in short supply in the market. [0003] The seeds of ice balls are small, the pollination rate is low under natural conditions, the seeds have no endosperm, they cannot provide the nutrients required for germination, and rarely germinate into seedlings. Due to over-exploitation in recent years, the resources of wild ice hockey are scarce, and with the deterioration of environm...

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Problems solved by technology

[0005] Although the above-mentioned scheme solves the deficiencies of the prior art to a certain extent, in the disclosed patent, the pseudobulb of the ice ball is used as the propagation material, and compared with the seed propagation, its propagation coefficient is small and the growth rate is slow; in Liao Tingting et al. In the study, the growth medium of the tissue bud block explants could not jointly promote the seed germination and seedling growth of the ice ball, and the reproduction coefficient was small, and it could not inhibit the growth of miscellaneous bacteria; in the research of Youchao et al., the seed germination rate Low, the explant medium is easy to be infected with miscellaneous bacteria

So far, there is no report about the substratum that can promote the germination of ice ball seeds, shorten the germination time of seeds, improve the germination rate of seeds and inhibit the growth of miscellaneous bacteria on the substratum

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