51results about How to "Promote germination and growth" patented technology

The invention relates to a pest trapping device and a preparation method and application thereof. The pest trapping device comprises a hard colored pest attracting board, a cingula with infective pests, and hanging objects with both attractants and sustained-releasing agents, wherein the hard colored pest attracting board is folded into a box of which the left end and the right end are opened; the cingula with the infective pests is placed at the bottom of the inner side wall of the box; and the hanging objects with both the attractants and the sustained-releasing agents are hung at the top of the inner side wall of the box. The pest trapping device has the advantages that by use of the taxis of pests to colors and odors, and the pests at the room temperature are trapped in the pest trapping device and then stained with fungal conidial powder to be killed by infection. Besides, the body surfaces of some pests flying out of the pest trapping device carry conidial powder, so that spores are actively diffused outside to infect other pests, and accordingly, the purpose of continually controlling the pests is achieved.

The invention relates to a tissue culture method of Gentiana dahurica Fisch for rapid in vitro reproduction. The method comprises the following steps: (1) selection and sterilization process of explant, comprising taking the stem tip of seedling of biennial Gentiana dahurica Fisch in a greenhouse as the explant, dipping for sterilization and washing so as to obtain the sterilized explant stem tip; (2) inoculation of explant, comprising inoculating the explant stem tip after sterilization at a callus tissue induction culture medium so as to form callus tissue; (3) proliferation and differentiation culture of the callus tissue, comprising inoculating the callus tissue to a callus tissue proliferation culture medium to culture so as to form single buds of Gentiana dahurica Fisch; (4) induction of root growth, comprising separating the single buds of Gentiana dahurica Fisch and afterwards transplanting into the root growing culture medium to culture so as to form complete regenerated plantlets; (5) sterilization of seedling medium; (6) transplanting of plant, comprising transplanting the complete regenerated plantlets into the seedling substrate subjected to sterilization directly, and acclimating the seedling so as to grow normal Gentiana dahurica Fisch plants. According to the method, the problems of overlong seedling period and low reproduction coefficient of Gentiana dahurica Fisch are overcome, and the method is easy to operate and can be used for rapid factory seedling culture.

The invention relates to a composite microbial fertilizer capable of preventing the vegetable root-knot nematode disease and a preparation method thereof, and belongs to the field of fertilizer production. The composite fertilizer is produced by the following steps: adding carbon containing plant powder and cereal protein powder into a decomposed organic fertilizer to prepare an organic substance, adding functional bacteria into the organic substance, and fully and evenly mixing to obtain the novel multi-resistance composite fertilizer. The functional bacteria can breed massively around the roots of vegetables and crops to form circular protective covers, thus the diseases caused by root-knot nematode are prevented effectively, a good ecological environment is created for the growth of vegetables, at the same time, the soil environment is improved, the soil hardening is avoided, the ventilation and oxygen content of soil are improved, the product quality and output are increased, and the fertilizer has the functions of pesticide and fertilizer. The organic substance content of the composite microbial fertilizer is not less than 50%, and the beneficial bacterium content is 6*10<9> CFU/g. The composite microbial fertilizer is easy to use, the fertilizing effect is high, the fertilizer only needs one application, labor is saved, and the environment is protected.

The invention discloses an ecological restoration method for an abandoned mine. The method comprises the following steps of S1, sampling and testing soil of the mine; S2, searching for a restoration soil source; S3, detecting the soil source, the pH of soil of the soil source and the content of calcium ions; S4, conducting acid mine restoration; S5, conducting alkaline mine restoration; S6, preparing a calcium ion solution; S7, conducting fertilization at regular time, wherein N fertilizer, P fertilizer, K fertilizer and other kinds of fertilizer can be applied at regular time to improve the soil fertility; S8, planting plants; S9, inoculating rhizobia, wherein the rhizobia are inoculated to the leguminous plants during planting; S10, introducing animals, wherein several groups of earthworms are freed in the soil source in the early stage; S11, observing restoration conditions. The restoration method is more scientific and reasonable, the soil activity is improved, improvement is accelerated, the mine restoration cycle is shortened, heavy metal ions in the mine can be enriched, germination and growth of the planted plants can also be promoted, and the structure, water permeabilityand ventilation of the soil can be improved.

The invention discloses a big-seedling container cultivation method for macadamia nuts, and belongs to the technical field of macadamia nut planting. The method includes the following steps of (1) seed selection, (2) seed pretreatment, (3) sowing, (4) rootstock seedling cultivation, (5) grafting, (6) grafted seedling management and (7) big seedling management; the survival rate of transplanting isup to 95%. Through a rootstock seedling combination cultivation method, field rootstock seedlings are grafted 3-4 months earlier than those in a traditional breeding method, staggered emergence is achieved in four batches, the production efficiency is improved, and the market demand is met. The big-seedling container cultivation method for the macadamia nuts is simple to operate and easy to master, seedlings are orderly, the survival rate is high, flowering and fruiting can be advanced by 1-2 years, the planting cycle is shortened, the management is convenient, and the cost is saved.

The invention discloses a rapid cultivation method of macadamia nut rootstocks. The method comprises the steps that 1, macadamia nut seeds are put into a 0.5%-0.8% trisodium phosphate solution for soaking before sowing is conducted, the seeds on the lower portion are taken out and washed through clean water, and then the seeds are sequentially placed under the conditions of four different stages; 2, the seeds obtained in the first step are put into a constant-temperature vibrator loaded with a second soaking solution, oscillating soaking is conducted for 15-20 h, and the temperature ranges from 40 DEG C to 45 DEG C; 3, sprouting cultivation is conducted on the soaked seeds on the condition that the temperature ranges from 35 DEG C to 38 DEG C and the relative humidity ranges from 80% to 85% till the width of natural cracking sprout holes of the seeds reaches 2.5 mm or above, and the seeds are taken out; 4, the seeds obtained in the third step are sown into a sand bed substrate and cultivated till the height from seedlings to the surface layer of the sand bed substrate reaches 20 cm or above and the stem thickness reaches 0.6 cm, and the seedling can serve as the rootstocks for grafting. The method can rapidly cultivate the rootstocks suitable for grafting propagation of macadamia nuts.

The invention discloses a method for culturing high temperature-resistant osmunda japonica seedlings. The method comprises the following steps: a step (1) of spore collection; a step (2) of spore pretreatment; a step (3) of seedling culture substrate preparation; a step (4) of spore seeding; and a step (5) and management after seeding. Each growth condition during osmunda japonica spore seedling culture is reasonably controlled and matched, temperature resistance characteristics of the seedlings are improved well, so that the seedlings can still grow well under the conditions of 30-35 DEG C, the germination rate of osmunda japonica spores can be remarkably improved, the germination duration is shortened, and the method has good popularization value.

The invention discloses a camellia oleifera grafting propagation method. The method includes steps: S1, scion selection, to be specific, taking semi-lignified spring shoots, growing in the current year, of valuable and rare or good-variety camellia oleifera trees as scions; S2, scion treatment, to be specific, soaking cut faces of the scions in a growth synergist at 15-25 DEG C for 0.5-1h, whereinthe growth synergist comprises 5-20Mug/L plant antibacterial peptides aqueous solution, 40-80mg/L lysozyme aqueous solution, 5-10mg/L 2-dodecylene diacid aqueous solution, 20-30mg/L compound sodium nitrophenolate aqueous solution and 100-150mg/L abelmoschus manihot extract aqueous solution; S3, rootstock selection and treatment; S4, grafting; S5, after-grafting management. By adoption of common camellia oleifera as rootstocks for grafting of valuable and rare or good-variety camellia oleifera, the population quantity of endangered valuable and rare camellia oleifera can be remarkably increased by means of vegetative propagation to guarantee normal annual rate of increase, and a rich camellia oleifera gene resource library can be established, such as camellia luteoflora which is a new species of camellia oleifera; high-yield, stable-yield, high-oil-yield and high-oil-quality camellia oleifera fruits can be obtained, such as Ganshi 84-8.

The invention discloses a seed coating agent for promoting germination and seedling formation of leguminous seeds in saline-alkali soil and a preparation method of the seed coating agent. The seed coating agent mainly comprises a soybean rhizobium solution, a bacillus liquid a, a bacillus liquid b, silk fibroin, peptone and the like. A film is formed outside the seeds before sowing, so that the seeds are helped to resist abiotic stress outside soil, and a protective layer can be formed around rhizosphere during seedling growth, so that the harm of abiotic stress to the seedlings is further reduced, and the leguminous seeds can grow healthily.

Compositions, admixtures and methods for resisting erosion such as by wind and/or water are provided. The compositions comprise an emulsion, dispersion or suspension of a hydrophilic polymer. In preferred embodiments, the polymer comprises branched anionic polyacrylamide. The composition may be combined with water and optionally a matrix material, to form an admixture that is applied to the soil or other material needing erosion resistance. The matrix material preferably comprises cellulose and/or mulch. The methods comprise providing or forming an aqueous mixture of the compositions and applying them to an area of land or material sought to be provided with erosion resistance.

The invention discloses an annual prevention and elimination method for weeds in a sugarcane field. According to the annual prevention and elimination method, in windless and sunshiny morning or evening, 90% acetochlor EC, 95% glyphosate powder, 24% clethodim EC, 20% glufosinate-ammonium aqua, washing powder and the like are used as the spraying agent, and are uniformly mixed with clear water according to a certain ratio; and the obtained mixture is sequentially sprayed to the sugarcane field three times for weeding at the critical growth stage of sugarcane and weed growth nodes of the sugarcane field, so the absence of weeds in the sugarcane field can be ensured all year. According to the present invention, the four agents are reasonably matched at the optimal weeding season in the sugarcane field, the weed removing rate can achieve more than 95% in 10 days after application of the agents, the labor and the cost are saved, the annual weed prevention effect can be achieved, the occurrence of plant diseases, insect pests, weeds and rats can be effectively reduced, and the all-year normal growth of sugarcane can be ensured.

The invention relates to the technical field of plant planting, in particular to a multi-flower globe amaranth planting method. The method includes the steps of transplanting, seedling period managing, growth vigorous period managing and flowering period managing. According to the growth characteristics of globe amaranth at different stages, by means of different fertilizer-water strategies, processing is conducted through nutrition glue and various types of nutrient solutions, the branching of the globe amaranth is promoted, the number of flowers is increased, and the color of the globe amaranth flowers is changed. The globe amaranth processed through the method has the average flower branch number of about 14 branches per plant and the average flower number of about 17 flowers per plant;meanwhile, the flower color of the globe amaranth is changed and presented as the bluish violet-acid blue color and is the color which ordinary globe amaranth does not have, and the ornamental valueof the globe amaranth is remarkably improved.

The invention discloses a lucid ganoderma wild-imitating cultivation method. The method comprises the steps that maple, radix fici hirtae, China wood oil wood, white oak, litchi trees, longan trees, schefflera octophylla, pistacia weinmannifolia, castanopsis fagaceae, fortune paulownia wood, wheat bran, cottonseed hulls and lime serve as a culture medium, then lucid ganoderma strains are inoculated, and lucid ganoderma fruiting fungus bags are cultivated; and soil, traditional Chinese medicine residues and a water-retaining agent are used as carriers to prepare an earthing substrate, leaves ofthe above wood are used as mushroom bed coverings, the lucidum ganoderma fruiting fungus bags are arranged and planted in a mushroom bed, and then are covered with the earthing substrate and the mushroom bed coverings, and finally lucid ganoderma is cultivated. By implementing the method, the prepared earthing substrate and the mushroom bed coverings are high in nutrient content, rich in humus, good in water retention property and good in air permeability, hyphae are massively propagated in the environment through cross covering of the earthing substrate and the mushroom bed coverings, and the hyphae are not only very good in quality but also grow rapidly. Lucid ganoderma sporocarp grows in the simulated wild environment, so that the yield and quality of lucid ganoderma are obviously improved, and the economic benefit is very remarkable.

According to the engineering bacterium capable of efficiently expressing chitinase and the application of the engineering bacterium, the engineering bacterium takes pichia pastoris GS115 as an expression host, and strategies such as gene copy number, cofactor co-expression and high-density fermentation are combined, so that the obtained chitinase has the advantages of high expression quantity, high activity and the like. The chitinase expressed by the engineering bacterium can hydrolyze high-concentration colloidal chitin, the hydrolytic activity is good, the hydrolysate is mainly N-acetylglucosamine and then N, N-diacetyl chitobiose, and the chitinase can be used for promoting germination and growth of rice and wheat seeds.

The invention discloses a cultivation method of pleurotus ostreatus, and relates to the technical field of cultivation of edible fungi. The method comprises the following steps of preparation of a culture medium material, bagging inoculation, cultivation of hyphae, fruiting management and harvesting. According to the method, by controlling the selection of a culture medium, the cultivation of thehyphae and the fruiting management, pleurotus ostreatus is scientifically managed and cultivated; by means of the method, the robust growth of the hyphae and the quick formation of sporophores are effectively promoted, the yield of the pleurotus ostreatus is increased, the quality of pleurotus ostreatus is improved, the production period is shortened, the bagging time of the hyphae can be shortened to 13-14 d, the hyphae are white, thick and dense, the fruiting time is 4-5 d earlier, the growth cycle is effectively shortened, cultivated pleurotus ostreatus is large in mushroom surface and robust and has good nutritional value and high economic benefits, and the content of amino acids, polysaccharides, mineral elements and the like is high.

The invention provides a microorganism for preventing and controlling apple root rot: Trichoderma harzianum HZD-5, Trichoderma koningii HZD-6, and also provides a microbial agent which is composed ofthe above two bacteria and used for preventing and controlling apple root rot. Research results show that the production method of the microbial agent is simple and the effect of preventing and controlling apple root rot is obvious; the microbial agent can effectively increase the disease resistance of apples; and there is no chemical residue due to addition of chemical drugs.

The invention discloses a method for improving a survival rate of the initial culture of passiflora caerulea. The method comprises the following steps: (1) initial culture medium preparation; (2) explant cultivation: selecting passiflora caerulea branches, cutting the branches into stem segments containing 1-2 buds, cutting off part of leaves, performing cuttage into a seedling-raising pot, performing placing into a greenhouse, spraying sterilization, desinsection and virus prevention and control medicines once during cuttage, spraying the sterilization, desinsection and virus prevention and control medicines once every 5-10 days, and taking the branches as explants for tissue culture after cultivation for a period of time; (3) explant disinfection: cutting leaves of the branches, cuttingthe branches into multiple segments, wherein axillary buds are located in the middle of the stem segments, and performing disinfection with a disinfectant containing HgCl2 and ascorbic acid; and (4) explant inoculation and culture. In the method, an explant material is cultured, the material is placed in the greenhouse for culture, pre-sterilization and other treatments are performed, the cleanliness of the explants is improved, and an HgCl2 disinfection time is shortened, so that a pollution rate of the initial culture is reduced to 15% or less. A browning rate of the explants is reduced, anda survival rate is increased.

The invention belongs to the technical field of tissue culture of traditional Chinese medicine plants, and particularly relates to a pleionebulbocodioides rolfe seed germination culture medium formulaand a preparation method. The pleionebulbocodioides rolfe seed germination culture medium formula is prepared from 1/2 MS or 1/4 MS, 0.5-2.5 mg/L of 6-BA, 0.1-0.3 mg/L of NAA, 0.1-0.25 g/L of GA, 40-50 g/L of a mashed banana, 3-15 g/L of a rosa roxburghii juice, 2-15 g/L of pueraria lobata, 1-15 g/L of a hawthorn, 2-10g / L of umbellate pore fungus, 1-10g / L of polygonum capitatum and 1-10g / Lof lonicera confusa. The formula has the advantages that the rosa roxburghii juice is used as one of preparation materials of the culture medium, other traditional Chinese medicine components are added on the basis, which can promote seed germination, shorten the germination time of pleionebulbocodioides rolfe seeds and increase the seed germination rate, and the formula can inhibit the growth ofhybrid bacterium on the culture medium compared with a conventional culture medium.

The invention discloses an osmunda japonica spore artificial breeding matrix. The breeding matrix is prepared from turfy soil, river sand, decomposed organic manure, paulownia wood sawdust, serpentine, stone needle, soybean lecithin, lycium barbarum polysaccharide, shrimp shell powder, seaweed gel, inorganic fertilizer, diethyl aminoethyl hexanoate, gibberellin and traditional Chinese medicine extracts. The prepared breeding matrix is rich in nutrient, reasonable in matching, loose and breathable in texture and high in water and fertility retention, accords with the growth and development rule of osmunda japonica spores, can remarkably increase the germination rate of the osmunda japonica spores, shortens germination duration, and improves quality of seedlings.

The invention relates to a hedgerow cultivation method, in particular to a honey locust hedgerow cultivation method. According to the technical scheme, in one month before honey locusts germinate, honey locust saplings with the diameter at breast height ranging from 1.5 cm to 2 cm are obtained and planted linearly in a single row at intervals ranging from 0.6 m to 0.7 m, after leaves fall in winter of the current year, trunks of the honey locust saplings are bent to the same side along the ranking line of the honey locust saplings from the portions 0.3 m away from the ground, after leaves fall in winter of the next year, all vertical lateral branches are bent in the bending direction of the original trunks from the portions 0.3 m away from the lateral branch base portions, and after the operation is repeatedly conducted for five years, winter pruning is conducted. According to the honey locust hedgerow cultivation method, the isolation effect of a hedgerow can be improved, disease and insect attack resistant capacity of the hedgerow is improved to a large extent, and the service life of the hedgerow is prolonged.

The invention discloses a Lentinus edodes cultivation technology. The Lentinus edodes cultivation technology comprises the steps of batching, sub-bagging, sterilization, inoculation, spawn running andfruiting, and further comprises a pre-wetting step between the batching step and the sub-packaging step; and the pre-wetting step is characterized in that a prepared dry culturing material containingold wood chips is pre-wetted before being bagged, and is stirred during watering, the pre-wetting temperature is controlled at 18-20 DEG C, the pre-wetting period is 2-4 days, and the water content of the obtained pre-wetted mixed culturing material is (60 +/- 3)% to make the culturing material absorb sufficient water and be sufficiently wetted. The pre-wetting step arranged before the sub-bagging and the control of the pre-wetting temperature at 18-20 DEG C promote the germination and growth of competitors, and the poor resistance of the competitors to high temperature and high humidity in the competitor growth and breeding stages is used to increase the sterilization efficiency.