Bacillus synergist and synergistic biocontrol bacterium agent

A technology of bacillus and biocontrol agents, applied in the directions of fungicides, biocides, biocides, etc., can solve the problem of reducing soil microbial synergists, etc., so as to weaken the antagonistic effect and negative impact, and achieve remarkable synergistic effect. Improve the effect of prevention

Active Publication Date: 2016-03-23
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on how to promote the germination and growth of bio-control bacteria and how to reduce the impact of soil microorganisms on bio-control bacteria

Method used

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  • Bacillus synergist and synergistic biocontrol bacterium agent
  • Bacillus synergist and synergistic biocontrol bacterium agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The bacillus synergist of this embodiment is composed of the following components in mass percentage: 50% of soybean meal, 15% of methyl cellulose, and 35% of sucrose.

[0023] The synergistic biocontrol agent of the present embodiment includes the following components in parts by weight: 5 parts of bacillus biocontrol agent and 1 part of bacillus synergist; the bacillus synergist consists of the following mass percentages Composition: soybean meal 50%, methyl cellulose 15%, sucrose 35%.

[0024] The Bacillus biocontrol bacterial agent is Bacillus amyloliquefaciens bacterial agent, which is the ZY-9-13 wettable powder developed by Zhengzhou Tobacco Research Institute of China National Tobacco Corporation, which is the original powder of ZY-9-13 bacterial agent It is prepared by mixing wood sodium, sodium lauryl sulfate, sodium alginate, sucrose and diatomaceous earth according to the mass ratio of 20:11:1:2:2:164; for details, refer to the records in the patent document...

experiment example 1

[0027] This experimental example detects the effect of the synergistic biocontrol agent obtained in Example 1. The test site is the tobacco experimental field in Biyang, Henan Province. The Bacillus amyloliquefaciens bacteria agent and clear water were used as the control. The tobacco roots were irrigated during the transplanting period and the early stage of the disease; the incidence and disease index of tobacco black shank were counted during the peak disease period to calculate the control effect. In addition, after the molecular labeling of the biocontrol bacteria, 3 days after the biocontrol agent was applied, the rhizosphere soil was taken to measure the number of biocontrol bacteria (microscopic fluorescence detection), and the survival rate of the biocontrol bacteria was calculated. The results are shown in Table 1.

[0028]Table 1 embodiment 1 gained synergistic biocontrol bacterium agent to the preventive effect of tobacco black shank and synergist to biocontrol bac...

Embodiment 2

[0032] The bacillus synergist of this embodiment is composed of the following components in mass percentage: 40% of soybean meal, 20% of methyl cellulose, and 40% of sucrose.

[0033] The synergistic biocontrol agent of the present embodiment includes the following components in parts by weight: 5 parts of bacillus biocontrol agent and 1 part of bacillus synergist; the bacillus synergist consists of the following mass percentages Composition: soybean meal 40%, methyl cellulose 20%, sucrose 40%.

[0034] The Bacillus biocontrol bacterial agent is Bacillus lysinus tobacco bacterial agent, which is Kpa strain wettable powder jointly developed by Zhengzhou Tobacco Research Institute of China National Tobacco Corporation and Fuzhou Company of Jiangxi Tobacco Company. It is made by mixing the original powder of the agent with wood sodium, sodium lauryl sulfate, sodium alginate, sucrose and diatomaceous earth according to the mass ratio of 20:11:1:2:2:164; for details, refer to the p...

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Abstract

The invention discloses a bacillus synergist and a synergistic biocontrol bacterium agent. The bacillus synergist is prepared from, by mass, 10%-80% of soybean pulp, 10%-80% of methylcellulose and 10%-80% of sucrose. According to the bacillus synergist, the soybean pulp, the methylcellulose and the sucrose are compounded, the bacillus synergist is used for a bacillus biocontrol bacterium agent, germination of spores of the bacillus can be promoted, and the survival rate of biocontrol bacteria is increased; the control effect of the biocontrol bacteria on disease bacteria is improved, the antagonistic action and negative effect of indigenous microorganisms on bacillus biocontrol bacteria are weakened, rapid germination and growth of the biocontrol bacteria are promoted, and the control effect of the biocontrol bacteria is improved; the synergistic effect of the bacillus synergist on the bacillus biocontrol bacterium agent is obvious through field experiments.

Description

technical field [0001] The invention belongs to the technical field of microbial synergists, specifically relates to a bacillus synergist, and also relates to a synergistic biocontrol agent using the above-mentioned bacillus synergist. Background technique [0002] Biocontrol bacteria refer to beneficial microorganisms used to control plant diseases. In nature, plant hosts not only closely interact with pathogens, but also extensively interact with surrounding non-pathogenic microorganisms. Microbial control of plant diseases refers to reducing the number of pathogens or weakening the pathogenic activity of pathogens by one or more microorganisms, thereby reducing the occurrence of diseases caused by pathogens. It is an important part of the biological control of plant diseases to prevent and control plant diseases by using the antibacterial effect of some antagonistic microorganisms on pathogens in nature, the competition effect of nutrients and space, the reparasitic effe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/00A01N63/00A01P3/00
CPCA01N25/00A01N63/00
Inventor 奚家勤薛超群万应发宋纪真孟庆华白建保王广山胡利伟牟文君尹启生郭建华刘芳
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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