90 results about "Indigenous microorganisms" patented technology

The invention relates to an activating agent and a method for repairing an organic contaminated site by targeted stimulation of indigenous microorganisms based on negative pressure infiltration promotion, which are characterized in that the activating agent containing nutrient substances, a sustained-release material and a surfactant is promoted to be uniformly distributed in the contaminated site through a negative pressure effect formed in soil by vacuumizing, so that the growth and activity of the indigenous microorganisms can be stimulated, and the effect of repairing the organic contaminated site by targeted stimulation of the indigenous microorganisms is achieved. The degradation of organic pollutants is accelerated. The defects that an existing in-situ organic pollutant site repairing technology is high in energy consumption, poor in effect, incapable of achieving secondary utilization of repaired soil, small in application range and the like are overcome, the provided activating agent can be combined with indigenous microorganisms in the site in a targeted mode, nutrient substances needed by growth are provided for the indigenous microorganisms, biodegradation of organic pollutants is promoted, and the effect of in-situ repairing of the organic pollutant site is achieved. And the activating agent is uniformly distributed in the polluted site under the negative pressure effect, so that more uniform and effective in-situ remediation is realized.

The invention discloses a method for repairing arsenic-cadmium combined polluted rice field soil, which comprises the following steps of: reducing the redox potential Eh value of the soil to-100 to-300mV by controlling the height of a flooded water layer of the polluted soil and adding a reducing agent, reducing arsenic in the soil into trivalent arsenic, and simultaneously constructing an indigenous microbial community with high arsenic methylation capability; oxidizing by draining and drying, methylating trivalent arsenic by indigenous microbial communities, and gradually volatilizing arsenic through methylation; and applying a carbon-based functional biological membrane mainly comprising a cadmium passivation flora to stably passivate cadmium. According to the method, the arsenic methylation capacity of microorganisms is improved by precisely regulating and controlling the Eh value, in-situ passivation of cadmium is enhanced through the synergistic effect of the carbon-based carrier and the composite flora, synchronous enhancement of arsenic methylation volatilization and cadmium biological membrane passivation is achieved, the difficulty that an arsenic and cadmium pollution treatment technology is'helpful 'is converted into an acting point of technical breakthrough, and the method is suitable for large-scale popularization and application. The arsenic and cadmium co-treatment of the combined polluted soil is realized.

The invention relates to an integrated grouting device and an offshore wind power single pile foundation anti-scour construction method based on an indigenous microorganism precuring seabed, and the method comprises the following steps: prefabricating a special integrated grouting device according to the installation condition of an offshore wind power single pile foundation; an integrated grouting device is installed on the seabed of the to-be-constructed area through a hammering method and a negative pressure method; urease microorganisms are enriched and cultivated in in-situ seabed soil through an integrated grouting device, the microorganisms are induced to generate calcium carbonate gelatinization crystals so as to bond sandy soil particles, and the overall strength of a surface seabed is improved; and a single-pile foundation is installed on the microorganism solidification seabed. Before pile sinking construction, the surface seabed of the to-be-constructed area is intensified in advance through in-situ enrichment cultivation of indigenous urease microorganisms and induction of mineralization and crystallization of the microorganisms, scouring protection of the single pile foundation in the whole service cycle is achieved, and then the local scouring problem in the pile sinking process is solved.

The invention provides a remediation method for hexavalent chromium polluted underground water, and relates to the technical field of water treatment. According to the preparation method, rice hulls are adopted as raw materials and treated through an oxygen-limited pyrolysis-ferric salt dipping composite technology, and the rice hull-based iron-rich biochar material is obtained; loading microorganisms with the rice hull-based iron-rich biochar material to obtain an adsorption material; the adsorption material is used for carrying out adsorption treatment on the hexavalent chromium-containing polluted underground water. Aiming at Cr (VI) polluted underground water, a prepared rice husk-based iron-rich biomass material is adopted to load domesticated soil indigenous microorganisms, and the domesticated soil indigenous microorganisms are added into a nearly neutral Cr (VI) polluted soil / underground water reaction system, so that efficient reductive degradation of Cr (VI) is realized, and the influence mechanism of Fe < 3 + > is explored.

The utility model discloses an isolation belt device for blocking and treating acid mine wastewater, and belongs to the technical field of mine wastewater isolation. The device comprises a buoy, an isolation belt fixing frame is arranged at the bottom of the buoy, a weight settling box is arranged at the bottom of the isolation belt fixing frame, and filler is filled in the weight settling box; biomass filler is arranged in the isolation belt fixing frame, and three-dimensional elastic brush filler is arranged on the front side and the rear side of the isolation belt fixing frame in a binding mode. The device disclosed by the utility model is simple in structure, can be effectively assembled according to site conditions, can be in contact with the bottom, realizes multi-depth treatment, can effectively reduce the concentration of dissolved oxygen converged into AMD, gradually improve the bottom layer environment, promote the growth of anaerobic microorganisms, perform biofilm formation synergistic treatment by using indigenous microorganisms and avoid the problem of adaptability of exogenous microbial agents, and can be effective for a long time; the cost and the risk of additionally adding nutrient sources and microorganisms are reduced.

According to the lysogenic phage community and the extraction method and application thereof, bacterial and phage communities are separated from in-situ soil in a medium proportion through a tangential flow technology, single bacterial communities and single phage communities are obtained through enrichment and concentration, meanwhile, mitomycin C is added into the single bacterial communities for lysogenic induction, and the lysogenic phage communities are obtained. And then filtering and enriching by using the tangential flow system to obtain a high-flux lysogenic phage community, and finally obtaining a single bacterial community, a bacterial-phage mixed community and a bacterial-lysogenic phage mixed community. The phage transplantation technology can promote indigenous microorganisms to participate in the plant growth and development process, and the transplanted lysogenous phage can participate in host plant hormone anabolism by optimizing a microbial network structure and injecting auxiliary metabolism genes. The method provides a long-acting environment-friendly microorganism synergistic growth promoting technology for green agriculture development in China.

The invention relates to a heavy metal polluted soil repairing and reinforcing method by utilizing indigenous microorganisms in cooperation with CO2 carbonization, which comprises the following steps: firstly, measuring indexes of heavy metal polluted soil, stacking the heavy metal polluted soil in different areas, and collecting specific polluted soil to test microbial indexes; preparing a polluted soil-urease bacterium suspension, and culturing to form a suspension; preparing an indigenous urease bacterium solution; preparing an indigenous urease bacterium liquid-urea mixed solution; then polluted soil is pre-repaired, a curing agent and the mixed solution are mixed, and particles are prepared and cured; and finally, high-concentration CO2 is introduced for reinforced carbonization repair, and the improved soil meeting the requirements is obtained. According to the method, indigenous microorganisms and CO2 carbonization are fully utilized, the method is especially suitable for repairing and reinforcing heavy metal polluted soil containing lead, copper, zinc and the like, the problems that a traditional solidification and stabilization method is high in energy consumption and poor in economical efficiency, and an existing microorganism mineralization technology is poor in microorganism adaptability and poor in repairing effect are solved, and the method is suitable for large-scale popularization and application. The method has the advantages of simple operation process, economy, effectiveness, low carbon and environmental protection.

The invention discloses an electrothermal coupling remediation method for water and soil integrated treatment of an organic polluted site, and belongs to the technical field of in-situ remediation of polluted sites. An electric field is arranged in a to-be-treated area, electric heating equipment is arranged between a cathode and an anode of the electric field, and a surface active agent added near the cathode and the anode, an oxidizing agent and a microbial electron acceptor are subjected to electrodialysis migration under the pulse electric impact effect of the electric field; and under the heating condition, desorption and dissolution of adsorbed pollutants are promoted, mixing collision with an activated oxidizing agent is promoted, electron acceptors conveyed by electromigration and heat synergistically improve the abundance and degradation activity of indigenous microorganism flora, soil residual and water-phase dissolved organic pollutants are synchronously degraded, and then electricity is utilized. And remediation is conducted in a dialysis, electromigration and thermal action coupling mode till the remediation target of the soil and the underground water is achieved, and water and soil integrated treatment on the organic pollution to-be-treated area is achieved.

The invention relates to the technical field of water quality treatment, and discloses a black and odorous water body treatment method which comprises the following steps: S1, placing and preparing a mixed injection mechanism; s2, stirring the water body to promote the water body to form upwelling flow; s3, adding a light-oxygen activation material into the water body; and S4, digesting pollutants in the water body, and activating indigenous microorganisms in the bottom mud and the water body. The technical scheme provided by the invention can solve the problems of unstable water quality, high investment cost, easy black and odorous return, possible secondary environmental pollution and the like in the existing black and odorous water body treatment technology.

The invention relates to the technical field of soil and underground water remediation, in particular to an in-situ microbial remediation method for organic underground polluted water and soil, which comprises the following steps: S1, separating indigenous microorganisms from the soil in a polluted area; s2, adding indigenous microorganism internal and external microorganism bacterium agents into a culture medium to obtain a mixed bacterium solution; s3, soaking a carrier into the mixed bacterial liquid, and performing shaking culture to obtain a carrier loaded with microorganisms; s4, the carrier loaded with the microorganisms is sown into the soil of the contaminated area, and remediation is completed after remediation is conducted for 30-40 days; and in the remediation process, underground water in the polluted area is pumped and sprayed into the soil. In the remediation process, underground water is pumped out for spraying, the soil water content is guaranteed, meanwhile, microorganisms can infiltrate along with the underground water, the underground water is remedied at the same time, the remediation time is shortened, and the remediation effect is guaranteed.

The invention relates to the technical field of soil remediation, in particular to an efficient in-situ microbial remediation method for organic contaminated soil, which comprises the following steps: layering and dividing the contaminated soil into layers and regions, and injecting different medicaments into circulating well groups positioned in different regions to achieve the purpose of adjusting an underground microbial environment, and automatically forming and constructing an aerobic-anaerobic transition zone. Meanwhile, self-circulation of underground water is accelerated through the circulating well group, different indigenous microorganism florae are activated, and a metabolic chain is formed; and aerobic / anaerobic microorganisms are periodically injected into the injection well, an electron donor and a carbon source are synchronously provided, the steady state of an ecosphere is maintained, and the aims of optimizing the construction period and the treatment effect of the in-situ bioremediation technology of the polluted soil and the underground water are achieved.

The invention provides a phosphorus release improving type compound fertilizer based on a fungal extracellular polymer, a preparation method and application, and belongs to the technical field of microorganisms, the phosphorus release improving type compound fertilizer is prepared by compounding the extracellular polymer generated by specific rhodotorula mucilaginosa WTSX01 and inorganic phosphate according to the mass ratio of 1: 9; the preparation method comprises the following steps: extracting and purifying high-activity EPS from a strain fermentation broth by a low-temperature dialysis method, separating lipid from EPS as a natural adhesive by a cosolvent extraction method, and finally mixing EPS, lipid and inorganic phosphate. The invention also discloses a synergistic application of the fertilizer and a phosphorus-solubilizing fungus aspergillus niger (CCTCC No. M2023240), so that the release efficiency of phosphorus in soil is improved; the fertilizer provided by the invention provides exclusive nutrition for phosphate-solubilizing microorganisms, effectively overcomes indigenous microorganism competition, remarkably promotes microorganism growth and phosphorus release, and realizes reduction and synergism of phosphate fertilizer and yield increase of crops.

The invention belongs to the technical field of petroleum hydrocarbon contaminated soil remediation, and particularly relates to a calcium peroxide pre-oxidation and microorganism synergistic remediation method for a petroleum hydrocarbon contaminated site. The method comprises the following steps that 1, 10-20 g / kg of calcium peroxide is added into soil, an activating agent is added, the water content of the soil is adjusted, pre-oxidation culture is conducted, and the pre-oxidation time is 20-25 days; 2) adding a conditioning agent, and adjusting the pH value of the soil to an interval range suitable for microbial growth; 3) applying a microbial nutrient in a spraying manner according to 1%-3% of the mass of the soil; and 4) adjusting the moisture content of the soil, maintaining the environment temperature at 24-26 DEG C under an aerobic condition, and culturing for 55-95 days. A small dose of activated calcium peroxide (CP) is adopted to perform pre-oxidation treatment on petroleum hydrocarbon pollutants in the soil, so that the activity of indigenous microorganisms can be enhanced, and the petroleum hydrocarbon polluted soil is remedied by cooperating with the indigenous microorganisms.

The invention discloses a method for constructing a small watershed non-point source pollution comprehensive control system, and relates to the technical field of ecological environment protection and treatment research. A small watershed needing non-point source pollution treatment is selected, and a pollution monitoring point scheme is determined from a preset monitoring station library according to geographical environment data and historical water quality data; digging a water channel, communicating the small watershed water system with the water channel, and enabling external water to flow into the water system; a small watershed water body is treated by adopting measures such as garbage floating object cleaning, algae killing and bottom mud degrading and repairing, so that the watershed water quality is improved within a short time. Further, selecting a proper area around the small watershed water body to construct a healthy water ecosystem, providing an adhesion matrix and a habitat for indigenous microorganisms to form a biological membrane, meanwhile, putting a proper amount of aquatic organisms to enable the water body to achieve a self-purification effect, and regularly detecting the water quality; and analyzing and evaluating the monitoring data through a preset non-point source pollution model to provide a decision basis for comprehensive treatment.

A lysogenic phage community, its extraction method and application. The tangential flow technology is used to proportionally isolate bacteria and phage communities from in-situ soil, and single-bacteria communities and single-phage communities are obtained through enrichment and concentration. At the same time, mitomycin C is added to the single-bacteria community for lysogenic induction, and then the tangential flow system is used again for filtration and enrichment to obtain a high-throughput lysogenic phage community. Finally, we obtain single-bacteria communities, bacteria-phage mixed communities, and bacteria-lysogenic phage mixed communities. The phage transplantation technology can promote the participation of indigenous microorganisms in the process of promoting plant growth and development. The transplanted lysogenic phages can participate in the synthesis and metabolism of host plant hormones by optimizing the microbial network structure and injecting auxiliary metabolic genes. This method provides a long-term and environmentally friendly microbial synergistic growth promotion technology for the development of green agriculture in China.

The application discloses an iron mine tailing pond wastewater recycling device, which comprises a slag slurry pump, a primary filter tank, a plurality of comprehensive filter barrels, a medicament tank, a microbial incubator and a clean water tank. An annular flow guide cover is arranged in the middle of the comprehensive filter barrel. A flow guide pipe extends from one side of the bottom of the comprehensive filter barrel to the upper part of the center of the flow guide cover. An annular first filter plate and a second filter plate are arranged on the outer side of the bottom of the flow guide cover from bottom to top. The second filter plate is slidably connected to the flow guide cover. A plurality of sludge collecting boxes are arranged at the bottom of the first filter plate for collecting the precipitated sludge. The application can stratify the precipitated sludge and retain the sludge with high microbial activity, so that the integrated operation of rapid sampling and subsequent cultivation of the sludge can be realized, and a new idea is provided for the indigenous microbial flora to treat the tailing pond wastewater.

The invention discloses a preparation method of a biomass-based slow-release agent for repairing polycyclic aromatic hydrocarbon polluted underground water, which comprises the following steps: 1, preparation of soybean milk fermentation liquor: weighing soybeans, soaking the soybeans in ultrapure water according to a solid-to-liquid ratio of 1: 20-1: 50 for 12-48 hours, pulping, and adding ultrapure water until the volume is 500mL to obtain a soybean milk matrix; inoculating 10mL of polycyclic aromatic hydrocarbon polluted underground water indigenous microorganism bacterial liquid into the soybean milk matrix, and carrying out closed fermentation under a constant-temperature oscillation condition, 2, preparation of a multifunctional composite slow-release agent: weighing 35-60 parts by weight of FeSO4. 7H2O, 1-5 parts by weight of rhamnolipid, 5-10 parts by weight of humic acid, 1-5 parts by weight of methionine, 5-10 parts by weight of peanut peptide, 1-10 parts by weight of soybean lecithin, 3-10 parts by weight of a yeast extract and 40-60 parts by weight of the soybean milk fermentation broth prepared in the step 1; the method has the beneficial effects that the degradation efficiency on organic pollutants such as polycyclic aromatic hydrocarbon is remarkably enhanced, and a reliable material basis and theoretical support are provided for engineering application of an underground water bioremediation technology.

The invention belongs to the technical field of plant planting, and particularly discloses a durian planting soil microbial flora improvement method, which comprises the following steps: collecting a target area soil sample at multiple points, and comprehensively detecting and analyzing the physicochemical properties and microbial flora composition of the soil; based on a detection result, screening beneficial indigenous microorganism strains and compounding; the compound flora is propagated by adopting a liquid submerged fermentation technology to prepare a microbial agent; the microbial agent is evenly applied to durian planting soil; and scientific fertilization management is synchronously matched while soil microbial flora improvement is implemented. Through fermentation culture and application of indigenous beneficial microorganisms in local soil, the indigenous beneficial microorganisms are high in environmental adaptability and easy to colonize in target soil, and the indigenous microorganisms are applied to durian planting soil, so that the flora structure of the durian planting soil is effectively restored, the physical and chemical properties of the soil are improved, and the fertility of the soil is improved.

The invention relates to the technical field of biological cells, and discloses a biologically fermented soil conditioner and a preparation method thereof.The soil conditioner is prepared from, by weight, 3 + / -2 parts of water dispersible nano sulfur-containing colloidal suspension, 70 + / -10 parts of compound amino acid solution, 20 + / -5 parts of compound ion mineral substance and 7 + / -2 parts of organic acid; according to the method, the soil conditioner is added into the soil, ionic mineral substances in the conditioner can rapidly promote ATP generation of indigenous microorganisms to promote microbial breeding gene change and shorten the breeding cycle, indigenous flora proliferation in the soil can be activated, the indigenous microorganisms are greatly proliferated to enable a soil layer to generate a fermentation effect, and the soil layer is protected from being damaged. The generated aerobic bacteria can multiply to kill anaerobic bacteria, and the multiplication of the aerobic bacteria not only can improve the soil fertility, potassium dissolving capacity, phosphorus dissolving capacity, salt dissolving capacity and nitrogen fixing capacity, but also can degrade heavy metal pollutants, so that the occurrence of soil-borne diseases is prevented and controlled, and the purposes of integrally repairing and improving the soil and increasing the crop yield are achieved.

The invention provides a method for preparing a camellia oleifera forest soil conditioner and a soil organic fertilizer based on indigenous flora and application of the method, and belongs to the technical field of microorganism utilization. According to the method, indigenous microorganisms captured from soil without fertilization interference under old camellia oleifera forests and healthy camellia oleifera trees in a germplasm resource nursery of more than one hundred years and indigenous microorganisms captured from broad-leaved forest or bamboo forest soil in a natural ecological system are combined according to a specific proportion; the oil-tea camellia forest soil conditioner or the oil-tea camellia forest soil organic fertilizer obtained through propagation culture can quickly improve and restore the oil-tea camellia forest soil in hills and mountainous areas and remarkably improve the yield and quality of oil-tea camellia seeds, is a solution based on nature, and is economical, practical, efficient, green, environmentally friendly and sustainable.

This invention relates to the field of food biotechnology and fermentation microbiology, specifically a method for obtaining lactic acid bacteria (LAB) isolates as starter culture candidates. The method is carried out by preparing a wheat-based fermentation substrate and adding gelatin protein as a nitrogen source to form a fermentation medium of wheat, gelatin, and fructose. Indigenous microorganisms in the substrate are inoculated into the fermentation medium and incubated under conditions that support the growth of lactic acid bacteria until lactic acid bacteria isolates are obtained. Fermentation is carried out at a temperature of 30–37°C for 12–72 hours, with a gelatin concentration of 10% (w / v) and a wheat to water ratio of 1:1 to 1:5 (w / v). The fermentation medium, a combination of wheat, gelatin, and fructose, functions as a selective medium for growing and selecting lactic acid bacteria before further isolation on standard microbiological media.This method produces lactic acid bacteria isolates that can be used as starter culture candidates in food fermentation processes.

The invention relates to a method for degrading petroleum hydrocarbon in soil by utilizing goethite reinforced endogenous microorganisms, goethite is used as a biological stimulant to activate the activity of soil endogenous functional bacteria for degrading petroleum hydrocarbon, dominant phylum of the soil endogenous functional bacteria is proteobacteria, actinomycetes and thick-wall phylum, and the relative abundance accounts for 50-80%. Under the conditions that the pH value of soil is 6.0-9.0 and the water content of the soil is 20%-50%, the degradation rate of total petroleum hydrocarbon in the soil is larger than or equal to 60%, and the degradation rate of components such as colloid and asphaltene is larger than or equal to 45%.

A method for producing methane through conversion of carbon in crude oil by microorganisms in an oil reservoir, comprising: S1: in the presence of a biological surface-active substance, promoting the degradation of crude oil in a crude oil produced liquid sample to generate a first product containing methane; and when the amount of methane generated in S1 is stable, performing S2; and S2: in the presence of the biological surface-active substance and directionally domesticated microorganisms, further degrading the first product and converting same into methane. According to the method, the effect of exogenous microorganisms can be synergized with indigenous microorganisms to form an oil recovery system that utilizes CO2 and crude oil to produce methane, and the efficiency of converting CO2 into methane can be overall improved by combining biotic factors with abiotic factors.

The invention belongs to the technical field of water quality restoration, and particularly relates to an iron-based-medical stone-persulfate composite restoration agent and a preparation method thereof. The high-dispersion and stable iron active center of the functionalized iron-based medical stone carrier can durably and efficiently catalyze persulfate to generate free radicals, ammonia nitrogen, nitrite and organic pollutants in a water body are rapidly oxidized and degraded, the active components are not easy to dissolve out or inactivate, and the oxidation efficiency is long-acting and stable. By designing the I-type assistant and the II-type assistant, the I-type assistant realizes rapid reduction of pollutants through gel slow release and microporous structure regulation and control in the initial stage of remediation. After the oxidant is used up, the anti-oxidation microcapsule wall material is gradually degraded, a nitrogen source, a carbon source, a phosphorus source and trace elements are slowly released, and growth and metabolism of indigenous microorganisms or specific florae are directionally stimulated, so that early-stage oxidation intermediate products are thoroughly mineralized into harmless substances. Meanwhile, the final product is high in mechanical strength and convenient to store, transport and add.

The present invention discloses a microbial remediation method applicable to uranium-contaminated groundwater, and relates to the field of water pollution remediation. The method comprises the following steps: conducting a uranium pollution survey on groundwater in a contaminated area, determining the contamination plume according to the uranium concentration, calculating the permeability coefficient of the rock and soil layer and the influence radius of the remediation well; constructing a plurality of remediation well positions so that the remediation areas of the plurality of remediation wells overlap and cover the contamination plume; adding a remediation agent in each remediation well, the remediation agent comprising sodium bicarbonate, sodium acetate, ethanol, ferrous sulfide and water. The present invention is applicable to the microbial remediation technology of uranium-contaminated groundwater, and the remediation agent considers three aspects, namely, regulating the environment, providing nutrition and preventing rebound, improving the living environment of microorganisms, stimulating and improving the biological reduction efficiency of heavy metal uranium by indigenous microorganisms in groundwater, and will not cause secondary pollution to the environment. The uranium contamination of groundwater can be continuously remediated, and the remediation area can be wide, and a large amount of manpower and material resources are not required, so the method is simple and economical.

The utility model discloses a kitchen waste treatment and display system based on a double-chamber microbial fuel cell. The kitchen waste treatment and display system comprises an anode, a cathode, a kitchen waste collection chamber, a water chamber, an ion exchange membrane, an electric signal collector, a display, a deodorization pipe, an aeration pipe and an air inlet device, the kitchen waste collecting chamber and the water chamber are communicated with each other and are separated by an ion exchange membrane; the anode is arranged in the kitchen waste collecting chamber, the cathode is arranged in the water chamber, the anode and the cathode are connected through a wire, and the wire is further connected with an electric signal collector; an aeration pipe is arranged in the water chamber and is connected with an air inlet device outside the water chamber. According to the kitchen waste treatment and display system disclosed by the utility model, indigenous microorganisms in the kitchen waste are used for carrying out anaerobic decomposition on the kitchen waste, and generated electrons reach the cathode through the lead and are subjected to reduction reaction with dissolved oxygen in water, so that current is generated; the public can perform aeration by participating in operation of the manual air inlet device and observe the increase of the electricity production quantity and the kitchen waste treatment quantity.

The invention provides a composite indigenous microorganism, a composite indigenous microorganism bacterium agent and a preparation method and application thereof, and belongs to the technical field of microorganism utilization. According to the compound indigenous microorganism disclosed by the invention, indigenous microorganism traps in typical disease-inhibiting soil which does not break out soil-borne diseases during long-term tobacco continuous cropping in a tobacco planting area are taken as a main body, so that a rhizosphere beneficial microorganism main force group which has natural immunity and is used for preventing and treating tobacco continuous cropping diseases is formed; soil indigenous microorganism traps in a natural reserve area which is close to a tobacco planting area and is relatively less interfered by human activities are used as necessary supplements, and beneficial microorganism groups of farmland soil disappearance caused by long-term use of chemical fertilizers, pesticides and the like are specifically supplemented, so that the disease prevention ability of the main force group is further assisted and improved. The compound indigenous microorganism disclosed by the invention can be prepared into a compound indigenous microorganism bacterium agent, a soil conditioner or a functional bio-organic fertilizer, can be used for effectively preventing and treating tobacco soil-borne diseases after being applied in a field, and has a good field effect.

The application discloses a method for constructing a small watershed non-point source pollution comprehensive prevention and control system, and relates to the technical field of ecological environment protection and treatment. A small watershed needing non-point source pollution treatment is selected, a pollution monitoring point scheme is determined from a preset monitoring station site library according to geographical environment data and historical water quality data, a water channel is dug, a small watershed water system is connected with the water channel, so that exogenous water can flow into the water system, and measures such as cleaning garbage floating objects, killing waterweeds, degrading and repairing bottom mud and the like are adopted to treat the small watershed water body, so that the watershed water quality is improved in a short time. Further, a suitable area around the small watershed water body is selected to construct a healthy water ecological system, an indigenous microorganism is provided with an attachment matrix and a habitat to form a biological membrane, and a proper amount of aquatic organisms is simultaneously put into the water body, so that the water body reaches a self-purification effect, and water quality is detected regularly; the monitoring data are analyzed and evaluated through a preset non-point source pollution model, so as to provide a decision basis for comprehensive treatment.

This invention discloses a method for remediating halogenated hydrocarbon pollution based on functional plasmid transformation, belonging to the field of environmental microbial remediation technology. The method first extracts plasmid DNA carrying functional genes from organobacteria with reductive dechlorination capabilities (organobacteria with halogen respiration). Then, the functional plasmid DNA and electron donors are directly added to halogenated hydrocarbon-contaminated samples containing indigenous microorganisms to construct a bio-enhanced remediation system. Finally, the system is cultured under anaerobic conditions to transform the functional plasmid into the indigenous microorganisms, thereby endowing or enhancing the indigenous microorganisms with the ability to reductively dechlorinate halogenated hydrocarbon pollutants. This invention avoids the ecological risks of directly introducing exogenous live bacteria. Through plasmid-mediated horizontal gene transfer, it rapidly activates and enhances the function of in-situ microbial communities, significantly improving the degradation rate and thoroughness of halogenated hydrocarbon pollutants, and providing an efficient, safe, and universally applicable technical means for in-situ bioremediation of contaminated sites.

The invention belongs to the technical field of petroleum hydrocarbon contaminated soil remediation, and particularly relates to a method for remediating petroleum hydrocarbon contaminated soil through combination of chemical pre-oxidation and indigenous microorganisms. The method comprises the following steps that 1, 10-20 g / kg of sodium percarbonate is added into soil, an activating agent is added, the water content of the soil is adjusted, pre-oxidation culture is conducted, and the pre-oxidation time is 15-20 days; 2) adding a conditioning agent, and adjusting the pH value of the soil to an interval range suitable for microbial growth; 3) applying a microbial nutrient in a spraying manner; and 4) adjusting the moisture content of the soil, maintaining the environment temperature at 24-26 DEG C under an aerobic condition, and culturing for 55-95 days. The petroleum hydrocarbon pollutants in the soil are subjected to pre-oxidation treatment by adopting a small dose of activated sodium percarbonate, so that the activity of indigenous microorganisms can be enhanced, and the petroleum hydrocarbon polluted soil is remedied by combining the indigenous microorganisms.