5 results about "Indigenous microorganisms" patented technology

This invention discloses a method for remediating halogenated hydrocarbon pollution based on functional plasmid transformation, belonging to the field of environmental microbial remediation technology. The method first extracts plasmid DNA carrying functional genes from organobacteria with reductive dechlorination capabilities (organobacteria with halogen respiration). Then, the functional plasmid DNA and electron donors are directly added to halogenated hydrocarbon-contaminated samples containing indigenous microorganisms to construct a bio-enhanced remediation system. Finally, the system is cultured under anaerobic conditions to transform the functional plasmid into the indigenous microorganisms, thereby endowing or enhancing the indigenous microorganisms with the ability to reductively dechlorinate halogenated hydrocarbon pollutants. This invention avoids the ecological risks of directly introducing exogenous live bacteria. Through plasmid-mediated horizontal gene transfer, it rapidly activates and enhances the function of in-situ microbial communities, significantly improving the degradation rate and thoroughness of halogenated hydrocarbon pollutants, and providing an efficient, safe, and universally applicable technical means for in-situ bioremediation of contaminated sites.

The present application belongs to the technical field of groundwater pollution remediation, and particularly relates to an in-situ biological stimulation remediation method for treating Cr(VI) and organic compound contaminated groundwater. The method is to use sugarcane syrup as a remediation reagent to stimulate the growth and domestication of indigenous microorganisms, so as to effectively remove the compound pollution in the groundwater. The sugarcane syrup, a by-product of sugar industry, has the best effect on removing the compound pollution of Cr(VI) and nitrobenzene (the removal rate can reach 99%). The present application also determines the removal conditions of the compound pollution of Cr(VI) and nitrobenzene: pH is greater than or equal to 7, temperature is greater than or equal to 15 DEG C, Cr(VI) is less than or equal to 35 mg / L, and the concentration of nitrobenzene is less than 75 mg / L. Under the conditions, the removal rate of Cr(VI) can reach 99%, and the removal rate of nitrobenzene can reach 95%. The present application provides an innovative solution for simultaneously remedying the groundwater contaminated by Cr(VI) and organic compounds.

The application discloses a sand land carbon fixation and ecological collaborative improvement method and system based on new carbon collection materials, relates to the field of soil improvement and ecological restoration, and specifically relates to a sand land carbon fixation and ecological collaborative improvement method and system based on new carbon collection materials. The method comprises the following steps: base construction, mixing new carbon collection material products A and B with sand in a dynamic ratio to form an artificial soil base layer with water and fertilizer retention capacity, and storing carbon in coal; system activation, activating indigenous microorganisms by applying bioactive agent product D, and combining with nutrient slow-release body product C to construct rhizosphere microecology; carbon sink monitoring and certification, monitoring soil organic carbon by means of the Internet of Things and certifying according to international standards; zero-carbon agricultural product production, using the system material as the main fertilizer to produce zero-carbon agricultural products. The new carbon collection material is prepared from low-rank coal as raw material through low-temperature catalytic conversion and the like, and contains more than 25 kinds of small-molecule organic acid complexes. The system contains preparation, application, monitoring and certification, and production modules, and realizes sand land carbon fixation and repair and zero-carbon production.

This invention relates to a method for in-situ enhancement of humic components in paddy field surface soil driven by periwinkle organisms. The method includes: crushing rice straw and soaking it in a periwinkle organism culture medium for a predetermined time; drying the soaked rice straw and spreading it on the paddy field surface after rice transplanting; controlling the light conditions on the field surface and implementing periodic short-term drying management during the rice tillering to booting stage. This invention utilizes an adsorbent straw-induced matrix to induce the rapid and directional colonization of periwinkle organisms, an aggregate of indigenous microorganisms at the paddy field soil-water interface, in situ, rather than exogenously adding microorganisms. This method offers high ecological safety and can simultaneously increase the content of humus, total organic carbon, and dissolved organic carbon in the paddy field surface soil within a single rice growing season. The method is simple to operate and suitable for large-scale application.

This invention discloses a light-driven method that utilizes straw biochar combined with activated iron minerals to regulate free radical generation at the soil-water interface in paddy fields or riverbanks, thereby degrading indirect photodegradable organic pollutants. This method employs a co-construction remediation system of straw biochar, activated iron minerals, and indigenous microorganisms at the interface to synergistically degrade organic pollutants that are difficult to photodegrade under sunlight. Bioremediation is achieved by constructing characteristic iron-reducing bacteria and organic matter-degrading bacteria at the soil-water interface, while chemical oxidation is accomplished through photochemical free radical generation from iron-containing minerals, and electron transfer is regulated by iron cycling mediated by straw biochar. This method achieves enhanced bio-photochemical coupling degradation of indirect photodegradable organic pollutants without the need for external hydrogen peroxide or persulfate. This invention is convenient to operate, low in cost, has good treatment effect, high degradation efficiency, is environmentally friendly, and has wide applications. It is a green remediation technology with broad application prospects and high practical value in the field of environmental remediation.