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Bacillus cereus TH-35 capable of activating heavy metal cadmium in soil and application thereof

A technology of Bacillus cereus and heavy metals, which is applied in the field of biological treatment of soil pollution, can solve problems that have not been reported, and achieve the effects of simple production process, improved efficiency, and accelerated absorption

Inactive Publication Date: 2016-11-30
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there is no report on the development of the function of activating soil heavy metal cadmium by using Bacillus cereus with rhizosphere growth-promoting effect screened from farmland soil.

Method used

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  • Bacillus cereus TH-35 capable of activating heavy metal cadmium in soil and application thereof
  • Bacillus cereus TH-35 capable of activating heavy metal cadmium in soil and application thereof
  • Bacillus cereus TH-35 capable of activating heavy metal cadmium in soil and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Isolation and Identification of Bacterial Strains

[0027] A high-efficiency cadmium-soluble Bacillus cereus provided by the invention is screened in the following manner. The rhizosphere soil of amaranth polluted by heavy metal cadmium was collected, and 10 grams of fresh soil samples were weighed in a 250 mL Erlenmeyer flask containing 90 mL of sterile water and glass beads according to the plate dilution method to make a bacterial suspension and diluted to 10 -4 -10 -6 times, smear on solid medium, and culture upside down at 28°C for 3-5 days. After a single colony grew on the plate, a single colony with an obvious phosphorus-dissolving circle was picked and streaked for purification (Figure 1), and a bacterium was isolated and numbered TH-35. The strain was deposited in Guangdong Microbial Culture Collection Center (GDMCC) on June 13, 2016, and the preservation number is GDMCC No.60044.

[0028] Scanning electron microscope observation and identificatio...

Embodiment 2

[0031] Example 2 Determination of the Activation Ability of Bacterial Strain TH-35 to Cadmium Carbonate

[0032] The screened bacterial strain TH-35 has a better effect of activating cadmium carbonate through the following experiments. Stored on a slope at 4°C Bacillus cereus TH-35 was activated, inoculated on the activation medium, and cultured for 24 hours. Pick the cadmium-dissolving bacteria on the activation medium and inoculate it in the enrichment medium, culture at 28°C, 150r / min, for 14-18h. Obtain the enrichment solution or make a bacterial agent, and the number of bacterial cells can reach 10 by plate counting 10 CFU / ml. The activation medium is broth peptone medium. Inoculate the enrichment liquid at 2% in cadmium-dissolving medium (without water-soluble cadmium ions), and incubate in a constant temperature incubator at 28°C and 150r / min for 3 days, and set a sterile control at the same time. After 3 days, the culture was terminated, and the culture solution ...

Embodiment 3

[0033] Embodiment 3: Determination of the ability of bacterial strain TH-35 to activate cadmium phosphate

[0034] The screened bacterial strain TH-35 has a better ability to activate cadmium phosphate through the following experiments. Bacteria stored on slant at 4°C Bacillus cereus TH-35 was activated, inoculated on the activation medium, and cultured for 24 hours. Pick the cadmium-dissolving bacteria on the activation medium and inoculate it in the enrichment medium, culture at 28°C, 150r / min, for 14-18h. Obtain the enrichment solution or make a bacterial agent, and the number of bacterial cells can reach 10 by plate counting 10 CFU / ml. The activation medium is broth peptone medium. The enrichment solution was inoculated in cadmium-dissolving medium (without water-soluble cadmium ions) at a volume ratio of 2%, and cultured in a constant temperature incubator at 28°C and 150r / min for 3 days, and a sterile control was set at the same time. After 3 days, the culture was ...

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Abstract

Belonging to the technical field of soil pollution biological treatment, the invention in particular discloses a Bacillus cereus TH-35 strain capable of activating heavy metal cadmium in soil. The strain is preserved in Guangdong microbiology culture center (GDMCC) on June 13, 2016, and the preservation number is GDMCC No.60044. According to the invention, an indigenous microorganism with efficient cadmium activation function is screened out from farmland soil, and is utilized to activate soil mineral nutrients at the rhizosphere and promote plant growth, at the same time, the indigenous microorganism also can effectively dissolve soil carbonate bounded forms, phosphate bounded forms and other major bounded forms of cadmium, thus effectively improving the efficiency of remediation of soil heavy metal cadmium by hyperaccumulator plants. In the process of activating soil heavy metal cadmium with the strain, no emission pollutant unfriendly to the environment is generated, therefore the Bacillus cereus TH-35 strain has great application prospects in preparation of environment friendly soil conditioners.

Description

technical field [0001] The invention belongs to the technical field of soil pollution biological treatment, and more specifically relates to a Bacillus cereus capable of activating heavy metal cadmium in soil ( Bacillus cereus ) TH-35 and its application. Background technique [0002] With the development of modern industry, the problem of soil quality deterioration caused by heavy metals entering the environment is becoming more and more serious. According to the survey by the Ministry of Agriculture, the area of ​​land polluted by heavy metals in my country accounts for 64.8% of the total area of ​​sewage irrigation areas, seriously threatening agricultural production and human health. [0003] Phytoremediation of heavy metal contaminated soil has gradually attracted people's attention due to its advantages of economy, environmental friendliness, and large amount of remediation. However, there are still many limitations in using plants, especially hyperaccumulators, to c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/085
CPCB09C1/10C12N1/20C12N1/205C12R2001/085
Inventor 王立立李取生何宝燕周雪芳周婷
Owner JINAN UNIVERSITY
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