High-temperature-resistant aerobic denitrification bacteria and application of same

A technology of aerobic denitrifying bacteria and high temperature resistance, applied in the field of environmental microorganisms, can solve problems such as increasing the cost of denitrification, and achieve the effect of avoiding environmental incompatibility and great application potential

Inactive Publication Date: 2015-08-12
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If room temperature aerobic denitrifying bacteria are used for biological denitrification treatment, cooling equipment must be installed, which increases the cost of denitrification

Method used

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  • High-temperature-resistant aerobic denitrification bacteria and application of same
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  • High-temperature-resistant aerobic denitrification bacteria and application of same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Isolation of high temperature resistant aerobic denitrification strains

[0025] Collect water samples, pour them into sterilized sampling bottles, store them at low temperature, and bring them back to the laboratory immediately, take 100ml of the water, add 0.2g of sodium citrate, K 2 HPO 4 0.1g, KH 2 PO 4 0.1g, KNO 3 0.2g, MgSO 4 ·7H 2 O 0.02g, adjust the pH to 7.2-7.4, namely the denitrification medium. Put the denitrification medium and 1mm sterile glass beads into a 500ml Erlenmeyer flask that has been sterilized at 121°C for 20 minutes, seal it with sterilized gauze, and place it on a shaker at 30°C at 200rpm for enrichment of aerobic denitrifying bacteria . After cultivating for about 72 hours, the degerm-free suspension was inserted into fresh sterile denitrification medium (configured with deionized water) according to the inoculum size of 10%, and enriched by continuous passage for three times, and the culture conditions were the same as abov...

Embodiment 2

[0027] Example 2: The denitrification ability of high temperature resistant aerobic denitrifying bacteria at different ambient temperatures.

[0028]Collect water samples and measure the pH, temperature, total nitrogen, nitrate nitrogen, nitrite nitrogen, ammonia nitrogen and total carbon of the water samples. Inoculate the aerobic denitrifying bacteria Pseudomonas stutzeri sp.strain N3 obtained above into the liquid medium, shake and culture at 30°C and 200rpm until the logarithmic phase of growth, centrifuge at 12000rpm for 5min under sterile conditions, and sterilize the 10mM sodium phosphate buffer After washing twice and resuspending, 10% of the inoculum was inoculated into the surface water sample of Meiliang Bay of Taihu Lake containing 20 mg / l nitrate nitrogen for the experiment. The experimental conditions are: KNO 3 Adjust C / N=8 with sodium citrate, nitrate nitrogen content is 20mg / l, pH7.2-7.4. Respectively in different ambient temperatures: 4-60°C, 200rpm shaking...

Embodiment 3

[0029] Example 3: Denitrification ability of aerobic denitrifying bacteria Pseudomonas stutzeri sp.strain N3 under high temperature environment.

[0030] Inoculate the aerobic denitrifying bacteria Pseudomonas stutzeri sp.strain N3 obtained above into the liquid medium, shake and culture at 30°C and 200rpm until the logarithmic phase of growth, centrifuge at 12000rpm for 5min under sterile conditions, and sterilize the 10mM sodium phosphate buffer After washing twice and resuspending, 10% of the inoculum was inoculated in the surface water samples of Meiliang Bay of Taihu Lake containing different concentrations of nitrate nitrogen for experiments. The experimental conditions are: using KNO 3 Adjust C / N=8 with sodium citrate, make the concentration range of nitrate nitrogen 20-100 mg / l, pH 7.2-7.4, shake culture at 50° C. and 200 rpm for 12 hours. Sampling was carried out to determine the content of nitrate nitrogen and nitrite nitrogen in the water body, and the removal rate...

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Abstract

The invention belongs to the field of environmental microorganisms and discloses a high-temperature-resistant aerobic denitrification bacteria, of which the classification name is pseudomonas stutzeri sp., wherein the strain has been preserved at China center for type culture collection at 2015 Jan.9 and has been assigned the accession number CCTCC NO: M2015027. The invention also discloses an application of the high-temperature-resistant aerobic denitrification bacteria. The strain grows well at 50 DEG C, has strong denitrification performance, is very less in accumulation of nitrite during denitrification and is free of secondary pollution. In the invention, the high-efficient aerobic denitrification bacteria are obtained through screening from a eutrophic lake water body, when being subjected to enlarged cultivation, the bacteria strain can be directly used for denitrification on lake water and can avoid environmental inadaptability of non-indigenous microorganisms. The bacteria strain can achieve huge economic benefit when being used in treatment of industrial waste.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and specifically relates to a high-temperature-resistant aerobic denitrification bacterium and its application in sewage denitrification. It is a kind of excellent microorganism breeding, thereby broadening the scope of treatment of eutrophic water bodies. Background technique [0002] Nitrogen is a very important pollutant in sewage, which can cause eutrophication of water body and endanger aquatic organisms and human health. Nitrogen in water mainly exists in two forms: organic nitrogen (such as protein, amino acid, etc.) and inorganic nitrogen (ammonia nitrogen, nitrate nitrogen and nitrite nitrogen). Among them, part of the organic nitrogen is assimilated and absorbed by aquatic organisms, and part of it is converted into ammonia nitrogen by ammonifying bacteria, and then through the nitrification of nitrifying bacteria and the denitrification of denitrifying bacteria, it is finall...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38
CPCC02F3/34C12N1/205C12R2001/38
Inventor 肖琳陈旭张小龙杨玉杨柳燕
Owner NANJING UNIV
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