31 results about "Calcium ion binding" patented technology

The invention provides a chlorella pyrenoidosa calcium chelating peptide and a chlorella pyrenoidosa peptide calcium chelate prepared by using the chlorella pyrenoidosa calcium chelating peptide. The prepared chlorella pyrenoidosa peptide calcium chelate can enhance the maximum load and structural strength of the tibia of a mouse with osteoporosis. The chlorella pyrenoidosa calcium chelating peptide provided by the invention is combined with calcium ions to form a peptide-calcium chelate, and animal experiments prove that the peptide-calcium chelate can significantly improve the maximum load and structural strength of the tibia of a mouse with osteoporosis, and has a good effect of improving the bone biomechanical properties of the mouse with osteoporosis.

The invention relates to a zinc-based metal organic framework material as well as a preparation method and application thereof, 1, 4-bis (2-ethylimidazole-1-yl) terephthalic acid is taken as an organic ligand, transition metal zinc is taken as a forming body, and the zinc-based metal organic framework material is synthesized through a solvothermal method. The zinc-based metal organic framework material is combined with calcium ions to serve as a fluorescent probe to be used as a ratio fluorescent probe of doxycycline hydrochloride in a water system, and rapid detection of the content of doxycycline hydrochloride can be achieved. The method for determining the doxycycline hydrochloride in the water has the characteristics of quick response, high selectivity, high sensitivity, high accuracy and the like, and compared with the existing doxycycline hydrochloride detection technology, the method shows good market application potential due to low cost, high efficiency, high sensitivity, high selectivity and simple operation.

The present disclosure relates to the field of wastewater treatment technology, and in particular to a nucleation granulation process for calcium chloride recycling and enhanced fluorine resource recovery. This process uses calcium hydroxide and a small amount of calcium chloride as a crystallization inducer. Fluoride ions consume calcium ions in the calcium chloride during the process, leaving residual chloride ions in the treated wastewater. The treated wastewater is then circulated back to the wastewater. When calcium hydroxide is added again, the residual chloride ions combine with the calcium ions in the calcium hydroxide to form calcium chloride, which then reacts with fluoride ions to form calcium fluoride. This achieves the goal of calcium chloride regeneration, enhanced fluorine resource recovery, and reduced treatment costs.

The application discloses a method for recycling and utilizing a major repair slag, which comprises the following steps: reacting the major repair slag with a certain amount of calcium chloride and a small amount of hydrogen peroxide under a pressurized condition, so that lithium elements in the major repair slag enter into a solution, and soluble fluorine ions combine with calcium ions to generate calcium fluoride products; then, a leaching residue is separated by step-by-step flotation to sequentially obtain carbon concentrate, calcium fluoride and cryolite products, and a water solution is recycled to a flotation process; and the leaching solution is prepared into a high-value lithium carbonate product by impurity removal, concentration and hydrothermal treatment. The method can recover various valuable substances in the major repair slag with low energy consumption and high value, and realizes the recycling and utilization of the major repair slag.

According to the spirulina platensis calcium chelating peptide and the application thereof, spirulina platensis is pretreated at high temperature and high pressure and then subjected to enzymolysis, and compared with zymolyte obtained without high temperature and high pressure, the zymolyte obtained after high temperature and high pressure treatment has higher calcium chelating activity. The spirulina platensis peptide calcium chelate with high calcium content is prepared by optimizing the peptide-calcium ratio, temperature, pH and other conditions, and animal experiments prove that the spirulina platensis peptide calcium chelate can significantly improve the bioavailability of calcium ions in osteoporosis mice and increase the bone density. The spirulina platensis calcium chelating peptide provided by the invention is combined with calcium ions to form a peptide-calcium chelate, and animal experiments prove that the peptide-calcium chelate can remarkably improve the calcium absorption rate and the calcium retention rate of osteoporosis mice, improve the bioavailability of the calcium ions in the osteoporosis mice and increase the bone mineral density.

The application discloses a high-calcium wastewater calcium removal reaction device, and relates to the technical field of calcium removal of wastewater. The high-calcium wastewater calcium removal reaction device comprises a calcium removal box, a filter box and a filter net basket. The high-calcium wastewater calcium removal reaction device has the effect of adjusting the calcium removal performance, air is introduced into wastewater in the form of aeration, the amount of carbon dioxide in the wastewater is reduced, the carbonic acid balance is broken, the balance system in the water body continuously moves to the direction of generating CO3 2‑ , calcium ions are combined with the CO3 to generate calcium carbonate crystals, calcium removal is facilitated, the first stirring plate and the second stirring plate are reversely rotated to stir, calcium ions are fully combined with carbonate ions, the removal of calcium ions is accelerated, and the practicability is improved.

The utility model discloses a device for biologically solidifying construction waste, which relates to the technical field of constructional engineering and comprises a support, a humidity control maintenance bin is fixedly connected to the inner wall of the support, a solidifying mechanism is arranged on the support, a uniform spraying mechanism is arranged on the support, and the humidity control maintenance bin is fixedly connected to the inner wall of the support. By arranging the strain culture reactor, firstly, urease-producing bacteria are cultured in the strain culture reactor, a culture medium containing urea and calcium salt is supplied for proliferation, high-activity bacterial liquid is obtained, then to-be-cured building waste crushed aggregates such as waste bricks and stones and concrete particles are flatly laid in the humidity-control curing bin, and the humidity-control curing bin is used for curing the building waste. According to the mechanism, urea in a nutrient solution is promoted to be decomposed through urease generated by bacteria, carbonate ions are formed, then the carbonate ions are combined with calcium ions to precipitate calcium carbonate crystals, adjacent particles are cemented together, building waste stacks can be reinforced into blocks or roadbed materials without traditional cementing materials such as cement, the process is environmentally friendly, and the construction waste is recycled. And new engineering value is given to the construction waste.

The application provides a nano-enzyme carbonized product, which is obtained by carbonization curing of a carbonized product precursor; the carbonized product precursor comprises: 30-60 parts by weight of solid waste; 20-40 parts by weight of filling material; 1-15 parts by weight of carbonic anhydrase nano-enzyme; 5-15 parts by weight of water reducing agent; and 10-30 parts by weight of water. Compared with the prior art, the carbonic anhydrase nano-enzyme is added to accelerate the absorption of carbon dioxide and the conversion into carbonate in the carbonization curing process, thereby improving the mechanical strength and durability of the product. Meanwhile, the added carbonic anhydrase nano-enzyme can specifically accelerate the absorption of carbon dioxide and the rapid conversion into carbonate through enzymatic action. With the rapid conversion of carbon dioxide, the material continuously absorbs more carbon dioxide, the generated carbonate combines with calcium ions to generate calcium carbonate, which fills the pores of the product and forms an interpenetrating network structure, thereby improving the strength and durability of the product.

A zinc-based metal organic framework material and a preparation method and application thereof, with 1,4-bis(2-ethyl imidazole-1-yl) terephthalic acid as an organic ligand, and transition metal zinc as a forming body, the zinc-based metal organic framework material is synthesized through a solvothermal method.The zinc-based metal organic framework material, after being combined with calcium ions, is used as a fluorescence probe for a ratio fluorescence probe of doxycycline hydrochlorate in a water system, and rapid detection of the content of doxycycline hydrochlorate can be realized.The method has the characteristics of rapid response, high selectivity, high sensitivity, high accuracy and the like in the determination of doxycycline hydrochlorate in water, and compared with existing doxycycline hydrochlorate detection technologies, the method has good market application potential due to low cost, high efficiency, high sensitivity, high selectivity and simple operation.

The present invention provides a treatment agent for oilfield produced fluid and its application in oilfield development. The treatment agent for oilfield produced fluid includes a decalcifying agent, which includes 5 to 10 parts of soluble magnesium salt, 10 to 20 parts of soluble metaaluminate, 5 to 10 parts of soluble carbonate and 10 to 15 parts of chelating agent by weight. The soluble metaaluminate can inhibit the hydrolysis of bicarbonate ions and promote the ionization of bicarbonate ions. The soluble carbonate can combine with calcium ions in the produced fluid to generate calcium carbonate precipitation, which is adsorbed and removed by aluminum hydroxide colloid produced by the hydrolysis of metaaluminate, and the chelating agent can also capture calcium ions, and the synergistic effect of the two improves the stability of water quality. The soluble magnesium salt can adjust the ionic strength of the decalcifying agent system. The chelating agent can further improve the adsorption of calcium carbonate by the decalcifying agent, remove calcium carbonate scaling, and improve the stability of water quality. The treatment agent for oilfield produced fluid can also reduce the cost of sewage treatment.

A non-aqueous electrolyte secondary battery according to one embodiment of the present disclosure comprises: a cylindrical outer can having a bottom section at one end and an opening at the other end; an electrode body and a nonaqueous electrolyte accommodated in a body section of the outer can; and a sealing body that blocks the opening via a gasket. A groove section where the diameter of the outer can is smaller than that of the body section is formed between the opening and the body section, and a functional layer containing a compound to which calcium ions are bonded is disposed in at least part of a region comprising the groove section and the opening on the inner surface of the outer can.

The invention discloses a multifunctional infectious bone defect repair material and application thereof, the multifunctional infectious bone defect repair material comprises nano-hydroxyapatite as a carrier and a functional active substance loaded on the nano-hydroxyapatite, namely an antibacterial-immunoregulation-osteogenesis-promoting multifunctional peptide, the nano-hydroxyapatite is used as a common bone repair material carrier, and the nano-hydroxyapatite is used as a bone repair material carrier. The multifunctional peptide is loaded through electrostatic adsorption and calcium ion combination dual modes, and polypeptide functionalized nano-hydroxyapatite (P / nHA) is formed. Compared with the prior art, the preparation method has the advantages that the infectious bone defect repair material is provided, material construction is achieved in a simple mode and does not depend on compounding of multiple functional components, and multifunctional peptide modified nano-hydroxyapatite can achieve integration of multiple functions of infection control, immunoregulation and osteogenesis induction; the repair and regeneration of infectious bone defects are promoted.

The invention provides a method for analyzing the aggregation crystallization process of a calcium phosphate pre-nucleation cluster, which comprises the following steps of: doping a small amount of aggregation-induced emission organic molecules and rare earth ions into the calcium phosphate pre-nucleation cluster, and taking the aggregation-induced emission organic molecules and the rare earth ions as dual-fluorescent probes to participate in the subsequent evolution process. Organic molecules are combined with calcium ions in clusters, and along with gradual increase of local molecular density of PNCs aggregation organic molecules, limitation of intramolecular movement is further enhanced, energy loss of an excited state is reduced, so that an electron transition channel is opened, and a fluorescence signal enhancement phenomenon is shown. The electric dipole transition (hypersensitive transition) of the rare earth ions is sensitive to the asymmetry of the surrounding coordination environment, and the magnetic dipole transition is not sensitive, so that the chemical structure of the rare earth ions can be judged by using the ratio of the two. The aggregation-induced emission characteristic of organic molecules is utilized to respond to the cluster aggregation process, the hypersensitive transition characteristic of rare earth ions is utilized to respond to the coordination environment asymmetry, the aggregation crystallization process of PNCs is matched, and the biomineralization mechanism of PNCs is explained.

The purpose of the present invention is to provide a marker for diagnosing non-alcoholic fatty liver disease (NAFLD). The invention relates to a marker for diagnosing non-alcoholic fatty liver disease (NAFLD). The marker is at least one protein selected from the group consisting of endopeptidase, hydrolase, calcium ion binding protein, cytoskeleton protein, transferase, receptor binding protein, phosphatase, antigen binding protein, RNA binding protein, reductase and cadherin binding protein, or a gene encoding the protein; by means of the marker, the above problem is solved.

The invention discloses a method for generating ultra-stable functional foam in situ based on urease-induced calcium carbonate particles, and belongs to the technical field of water-phase foam preparation. Vegetable protein solutions such as soybeans and peas are mixed with urea and a calcium ion solution, natural urease in the vegetable protein is used for catalyzing the urea to be hydrolyzed into carbonate particles, and the carbonate particles are prepared into the ultra-stable functional foam. And the plant protein is further combined with calcium ions to generate nano-micron calcium carbonate particles in situ, so that foam is formed by rapid shearing under the foaming and foam stabilizing effects of the plant protein and the nano-micron calcium carbonate particles. The viscosity, the half-life period, the particle concentration, the growth speed and other properties of the prepared foam can be regulated and controlled by controlling the concentration of the raw materials and adding the ionic surfactant, and finally the functional foam with the long half-life period is prepared from the raw materials low in price and serves geological engineering construction.

The present application provides a euphausia superba calcium chelating peptide, which is an enzymatic polypeptide prepared by enzymolysis of euphausia superba meat with neutral protease and papain; a specific amino acid sequence thereof is EEDLER or IVELEEE. The present application also provides a calcium chelating peptide, which is prepared by chelating calcium ions with the polypeptide. The euphausia superba calcium chelating peptide provided by the present application combines with calcium ions to form a calcium peptide chelate, so that the calcium ions can always remain in a soluble state during digestion, especially in the intestinal tract, effectively relieving the precipitation of calcium ions in the intestinal tract, and overcoming the shortcomings of inorganic calcium, such as strong irritation, low absorption and utilization rate, and poor absorption effect.

The invention discloses a calcium ion binding protein marker combination for evaluating individual radiosensitivity and application of the calcium ion binding protein marker combination, and belongs to the technical field of biotechnology and nuclear radiation protection. Aiming at the technical problems that a genomics detection method and a functional experiment method for evaluating individual radiosensitivity have obvious limitation and the like at present, the invention is based on a C57BL / 6J mouse half lethal dose radiation experiment for the first time; a radiosensitivity prediction model based on the serum calcium ion binding protein combination (Ihh, NUCB1 and PROS1) is screened and established through a high-throughput mass spectrometry technology, and the clinical application value of the serum calcium ion binding protein combination (Ihh, NUCB1 and PROS1) as the radiosensitivity prediction marker is proved for the first time. A novel molecular marker is provided for professional radiation protection monitoring and formulation of a precise tumor radiotherapy scheme.

The invention discloses a sludge solidification and compression integrated method for a soft soil foundation, and particularly relates to the technical field of soft soil foundation solidification treatment.The method comprises the following steps of S1, microorganism screening and culture, S2, microorganism injection and nutrient solution supply, S3, electrodialysis assisted drainage and reinforcement, and S4, intelligent monitoring. Urea is decomposed by means of microbial metabolism to generate carbonate ions which are combined with calcium ions provided by calcium chloride, calcium carbonate precipitates are formed to fill gaps of the foundation, meanwhile, moisture in the foundation is accelerated to move to a cathode under the action of an electric field, positive ions are promoted to migrate to the cathode and are combined with the carbonate ions generated through microbial induction, and the effect of the electric field is improved. And the curing and compression of the foundation are accelerated, the period is short, the environmental protection property is good, and the damage to the foundation soil can be reduced.

The application discloses a protein UP5 of the Malpighian tubule of Euproctis pseudoconspersa and application thereof, the protein can be combined with calcium ions, and has a significant ability of inhibiting formation of calcium oxalate crystals, and can be used for preventing and treating calcium oxalate stones which are the most common kidney stones, so that a new strategy for treating kidney stones is expected to be provided, and the protein UP5 of the Malpighian tubule of Euproctis pseudoconspersa provides a new drug use.

The invention discloses an organophosphorus conversion and recovery method which comprises the following steps: step 1, adding a calcium-doped catalytic material into organophosphorus-containing wastewater, and complexing the catalytic material with organophosphorus to generate a complex containing a carbon-phosphorus bond; step 2, irradiating the complex with light, so that a carbon-phosphorus bond in the complex is cracked to obtain orthophosphate radical; and 3, combining the orthophosphate radical with calcium ions in the catalytic material, and further treating to realize the recovery of calcium phosphate. According to the scheme, organic phosphorus is subjected to photocatalytic oxidation, the efficiency is high, the high salt water influence resistance is high, the secondary pollution is low, the circularity is good, the excellent performance of adsorption and photocatalytic oxidation can be synergistically utilized, and the method is suitable for large-scale industrial production, popularization and application.

The invention discloses a yellow thorn moth Martens tube protein UP5 and application thereof, the protein can be combined with white energy and calcium ions, has a remarkable capability of inhibiting formation of calcium oxalate crystals, and can be used for preventing and treating the most common kidney stone-calcium oxalate stone, so that a new strategy is expected to be provided for treatment of kidney stone, and the application of the yellow thorn moth Martens tube protein UP5 is worthy of popularization and application. The invention provides a novel medicine application of the Mala tube protein UP5 of the Cannidosa hemsleyana.

The invention discloses a method for affinity separation and purification of serratiolysin extracellular metalloproteinase based on polyglutamic acid, and belongs to the technical field of bioengineering. According to the method, the characteristic that the serratiolysin extracellular metalloproteinase contains seven calcium ion binding sites is utilized, polyglutamic acid is taken as a specific polycarboxylic acid ligand, directional capture of target protease is realized through calcium ion mediated coordination, and the steps of gradient elution and the like are combined, so that the high-purity serratiolysin extracellular metalloproteinase is finally obtained. The purification multiple reaches 6.8 times, the recovery rate is 85.2%, the electrophoresis purity is larger than or equal to 99%, and the method is mild in operation, low in cost and suitable for industrial production.

This invention proposes a flame-retardant and heat-insulating cellulose composite aerogel, its preparation method, and its application. The method includes the following steps: dissolving cellulose, adding a phosphating agent to react and introduce phosphorus-containing sites that can bind to calcium ions onto the cellulose molecular chains, obtaining a cellulose precursor solution; injecting this solution into a mold, and then placing the entire solution in a regeneration system containing a calcium source and an alkaline regulator, allowing the cellulose to regenerate while calcium ions diffuse into the confined space of the wet gel, binding to the phosphorus-containing sites and undergoing a simultaneous mineralization reaction; solvent exchange and drying to obtain the flame-retardant and heat-insulating cellulose composite aerogel. The technical problem to be solved is to achieve excellent flame-retardant, heat-insulating, and sound-absorbing properties, as well as long-term structural stability, and to achieve a synergistic improvement of these properties through a green and efficient integrated process of regeneration-induced simultaneous mineralization.

The invention provides a method for selectively extracting lithium from lithium feldspar type waste glass ceramics. The method comprises the following steps: mixing the lithium feldspar type waste microcrystalline glass with a roasting agent, and carrying out roasting treatment to obtain a roasted material; wherein the roasting agent is calcium chloride; the roasted material is subjected to water leaching, and water leaching liquid and leaching residues are obtained; removing calcium from the water extract to obtain calcium-removed liquid and calcium-removed by-products; and carrying out lithium precipitation reaction on the calcium-removed liquid to obtain a lithium salt product. According to the method, calcium ions and lithium ions can be replaced in the roasting process, and meanwhile, a silicon-aluminum dissolution path is blocked through combination of a silicon-aluminum framework and the calcium ions. And secondly, SiO3 < 2-> is combined with Ca < 2 + > in the water leaching process, so that the leaching concentration of silicon is reduced. In addition, a calcium removal byproduct can be used as a high-added-value product. Therefore, the method provided by the invention is simple and reliable in process, low in cost, high in lithium leaching rate and selectivity and environment-friendly.

The invention provides a cat prescription food for improving lower urinary tract diseases and a preparation method thereof, and the cat prescription food for improving urinary tract calculi comprises the following components in percentage by mass: a grain-free hypoallergenic raw material, chicken oil, yolk powder, chicken liver powder, fish oil, a Chinese herbal medicine raw material, cellulose, casein hydrolysate and 1-10% of a premix. By using the cereal-free low-sensitivity raw materials, the health of intestinal tracts can be cared; by adjusting the proportion of calcium, phosphorus, magnesium and other minerals, mineral balance of urine can be maintained, and by matching with herbaceous plant ingredients such as semen plantaginis, urination is promoted, and the calculus risk is reduced; by adding citrate, the combination of citrate and calcium ions is promoted, a soluble calcium citrate compound is formed, and the crystallization and deposition of calcium oxalate can be reduced; by controlling the protein content and adding the acidifying agent, weak acid urine can be maintained, bacterial growth can be inhibited, and struvite crystal dissolution can be promoted.

The invention discloses a method for preparing a Prussian blue analogue material by using a microcapsule slow release technology and application, and relates to the technical field of sodium ion battery positive electrode materials. According to the preparation method, the solution B is coated in a mode that alginate radicals are combined with calcium ions to form microcapsules containing the solution B, the microcapsules containing the solution B are added into the solution A, so that the microcapsules coating the solution B are uniformly dispersed in the solution A, the solution B is slowly released, prussian blue analogue crystals are slowly formed in a reaction system, the precipitation rate of the prussian blue analogue is reduced, and the yield of the prussian blue analogue is increased. The problem of non-uniform reaction can be effectively solved, the crystal quality is improved, and defects and crystal water are reduced. According to the method, carbonate ions are introduced to convert the calcium alginate film into insoluble calcium carbonate precipitates, weak acid is added or carbon dioxide gas is introduced to convert the water-insoluble calcium carbonate precipitates into soluble salts, and the soluble salts are removed by washing.

The present application provides a Chlorella pyrenoidosa calcium-chelating peptide and a Chlorella pyrenoidosa peptide calcium-chelate prepared by using the Chlorella pyrenoidosa calcium-chelating peptide; the prepared Chlorella pyrenoidosa peptide calcium-chelate can enhance the maximum load and structural strength of the tibia of an osteoporosis mouse. The Chlorella pyrenoidosa calcium-chelating peptide provided by the present application combines with calcium ions to form a peptide calcium-chelate, and experiments on animals prove that the peptide calcium-chelate can significantly improve the maximum load and structural strength of the tibia of an osteoporosis mouse, and has a good effect of improving the bone biomechanical properties of an osteoporosis mouse.

The invention provides a calcium ion binding peptide, a peptide-calcium chelate and application of the peptide-calcium chelate. The calcium ion binding peptide has at least one of the sequences as shown in SEQ ID NO: 1 to SEQ ID NO: 8. The peptide-calcium chelate is formed by chelating at least one of the sequences as shown in SEQ ID NO: 1-SEQ ID NO: 8 and Ca < 2 + >. The provided peptide-calcium chelate can be used as a calcium supplement, thereby treating calcium deficiency.

The invention relates to the technical field of rare earth element detection, in particular to a fusion protein and application thereof in rare earth ion detection. The calcium ion binding protein, the fluorescent protein and the LanM protein are fused, the obtained fusion protein is used for quantitative detection of rare earth ions in a water sample, the detection limit is low, the accuracy is high, the rare earth ion concentration magnitude can be accurately measured, and interference of non-rare earth elements in the environment can be eliminated.