11 results about "Biphasic calcium phosphate" patented technology

The application provides a kind of load exosome composite hydrogel and preparation method thereof, belong to biomaterial and drug delivery technical field;Its preparation method includes: modified chitosan;Modified hyaluronic acid;Preparation of biphasic calcium phosphate nanoparticles;Preparation of composite hydrogel.The application is through nucleophilic ring-opening reaction of 1,2-epoxy butane and the hydroxyl group of chitosan, form hydroxybutyl ether bond, give hydroxybutyl chitosan temperature sensitive characteristics, then, again, after activation 3,4-dihydroxyphenylalanine is condensed with hydroxybutyl chitosan, form amide bond, to graft 3,4-dihydroxyphenylalanine on hydroxybutyl chitosan, after modification of chitosan, 3,4-dihydroxyphenylalanine can form strong adhesion interface with cartilage, synovial membrane and other tissues through hydrogen bond, pi-pi stacking and metal coordination, thereby effectively prolonging the residence time of hydrogel in joint.

The invention discloses a cation-doped biphase calcium phosphate material and a preparation method and application thereof.The preparation method comprises the steps that a chemical precipitation method is adopted, the reaction process is precisely regulated and controlled, firstly, a reaction is carried out in an acidic microenvironment with the pH being 2-6, then aging is carried out under the alkaline condition with the pH being 9-12, and a two-step pH precise control strategy that acidity is carried out first and then alkalinity is carried out is adopted, so that the cation-doped biphase calcium phosphate material is obtained. According to the strategy, thermodynamic interference of cation doping on the HA / beta-TCP phase proportion can be effectively counteracted, and stable control over product phase composition is achieved. The preparation method has the core advantages that the proportion of hydroxyapatite (HA) to beta-tricalcium phosphate (beta-TCP) in the prepared biphase calcium phosphate material is highly stable, and the proportion does not obviously fluctuate in a specific range of the cation doping amount due to the change of the doping amount or cation types; the problem that the phase proportion is uncontrollable due to doping in the prior art is solved.

The invention relates to a biphase calcium phosphate / hydroxyapatite bone substitute material and a preparation method thereof. The bone substitute material comprises a degradable calcium phosphate core layer, a porous hydroxyapatite precursor layer and a hydroxyapatite gradient layer, wherein the porous hydroxyapatite precursor layer is formed on the surface of the degradable calcium phosphate core layer in situ through a sol-gel method, and the hydroxyapatite gradient layer is formed through electro-deposition treatment and covers the porous hydroxyapatite precursor layer. According to the technical scheme, a layered mineralization structure with a porous hydroxyapatite precursor layer as a template is constructed, and a hydroxyapatite layer with a continuous gradient in the thickness direction is formed through segmented electro-deposition treatment, so that the obtained composite material realizes continuous transition from outside to inside in the aspects of porosity, grain size, crystallinity and Ca / P ratio. The structure of the biphase calcium phosphate / hydroxyapatite bone substitute material can remarkably improve the interface bonding strength, the ion exchange capacity and the biological activity, so that the osteoblast adhesion, proliferation and osteogenic differentiation capacities are improved, and the overall osteogenic performance is obviously superior to that of an existing material.

The application relates to the technical field of biomedical materials, and specifically discloses a preparation method of a double-crosslinked 3D-printed bone repair scaffold. The scaffold is made of a biphasic calcium phosphate matrix and a gelatin methacrylate hydrogel outer layer. The preparation method comprises the following steps: firstly, preparing biphasic calcium phosphate ink, and constructing a porous scaffold blank through 3D printing; then, carrying out double-crosslinking treatment of calcium chloride solution and glutaraldehyde solution in sequence to obtain a scaffold matrix with enhanced mechanical properties; then, preparing gelatin methacrylate hydrogel loaded with modified teriparatide, and compounding the gelatin methacrylate hydrogel with the scaffold matrix; and finally, obtaining the final product after blue light crosslinking and solidification. The bone repair scaffold has good biocompatibility and osteogenic activity, and can effectively promote bone defect repair. In addition, the preparation method is controllable and has good repeatability, the structure of the scaffold is accurately controlled through the 3D printing technology, and a new approach is provided for the preparation of personalized bone repair materials.

The preparation method comprises the following steps: mixing strontium carbonate, zinc oxide, calcium carbonate and calcium hydrophosphate, carrying out ball milling to obtain powder slurry, drying, sintering, and carrying out wet ball milling to obtain strontium / zinc co-doped beta-TCP powder; the preparation method comprises the following steps: mixing calcium hydrophosphate with calcium carbonate, ball-milling into powder slurry, drying, sintering, crushing and grinding to obtain tetracalcium phosphate powder; and carrying out three-dimensional mixing on the strontium / zinc co-doped beta-TCP powder, calcium hydrophosphate and tetracalcium phosphate to obtain the strontium-zinc co-doped biphase calcium phosphate bone cement powder. According to the invention, through collaborative design of composition and structure, multifunctional active regulation and control of the bone repair process are realized.

The utility model discloses a skull repairing mesh plate, and relates to the technical field of medical implants, the skull repairing mesh plate comprises a mesh plate main body, the mesh plate main body has a curved surface shape matched with a skull defect area; the screen plate main body is made of a composite material of polyether-ether-ketone and biphase calcium phosphate; the mesh plate main body comprises a compact area and a loose area, and the porosity of the compact area is smaller than that of the loose area; every two adjacent loose areas are separated through the corresponding dense areas, and the dense areas are staggered to form a net-shaped structure. First micropores are formed in the surface of the loose area; first through holes are formed in the compact area at intervals, and the first through holes are formed in the thickness direction of the screen plate body in a penetrating mode. The skull repairing screen plate has good mechanical properties; the bone integration capability is good; due to the lightweight design, foreign body sensation after implantation is reduced; the occurrence rate of subcutaneous hydrops can be reduced.

The present invention relates to a two-stage sintering method for preparing a porous biphasic calcium phosphate ceramic from calcium-containing biological waste, wherein hydroxyapatite prepared from calcium-containing waste is mixed with a foaming agent to prepare a bone graft material having medicinal use through two-stage sintering.

This invention discloses a calcium phosphate ceramic particle-methacrylated gelatin hydrogel scaffold for osteoblasts pretreated with Tideglusib to enhance osteogenic differentiation, relating to the fields of biomedical engineering and bone tissue regeneration. It comprises osteoblasts pretreated with Tideglusib and activated, biphasic calcium phosphate ceramic particles, and a methacrylated gelatin hydrogel encapsulating the osteoblasts and ceramic particles. The preparation method includes: obtaining rat osteoblasts; seeding the osteoblasts onto the biphasic calcium phosphate ceramic particles; and pretreating the seeded biphasic calcium phosphate ceramic particles with Tideglusib solution to activate the Wnt signaling pathway and promote osteogenic differentiation. This invention exhibits excellent bone defect repair capabilities, good degradability and biocompatibility, provides necessary mechanical support, and has strong clinical translational potential.

The invention belongs to the technical field of bone tissue repair, and particularly relates to a bone tissue engineering scaffold for critical dimension bone defect repair and a preparation method. Comprising the following steps: preparing a BCP hollow spherical structure scaffold, carrying out surface mineralization modification by using an in-situ mineralization technology, and screening a mineralization modification structure for modification design; constructing a three-dimensional delivery system of the BMSCs by taking GelMA as a carrier; and constructing the 3D printing BCP hollow spherical mineralization modification composite scaffold loaded with the BMSCs three-dimensional delivery system. According to the invention, a digital light treatment photopolymerization 3D printing technology is utilized, a biomimetic raw material biphase calcium phosphate is based on components, a hollow spherical structure modification strategy is innovated, an in-situ mineralization technology is utilized for surface modification, and bone marrow mesenchymal stem cells are loaded through a three-dimensional delivery system, so that a new idea is provided for BTE design, and a new thought is provided for clinical treatment of bone defects.

An efficient biphasic calcium phosphate coating method. A high-concentration (4.5×) supersaturated calcium phosphate solution (SCPS) is used for coating and a thicker continuous coating is formed on a titanium disc. The coating contains dicalcium phosphate dihydrate and hydroxyapatite. The coating has improved encapsulation efficiency of a protein FITC-BSA and releases less Ca2+ under an acidic condition. A more efficient coating with a high yield and a protein encapsulation efficiency can be obtained with a less SCPS solution volume and a shorter coating time, such that a cost is greatly reduced in clinical application and the coating is very expected to be used in an osteogenic implant material in the biomedical field.

The invention relates to the technical field of biomedical materials, and particularly discloses a preparation method of a double-crosslinking 3D printing bone repair scaffold. The scaffold is prepared by compounding a biphase calcium phosphate matrix and a gelatin methylacrylate hydrogel outer layer. The preparation method comprises the following steps: firstly, preparing biphase calcium phosphate ink, and constructing a porous scaffold blank through 3D printing; sequentially carrying out double crosslinking treatment on a calcium chloride solution and a glutaraldehyde solution to obtain a scaffold matrix with enhanced mechanical properties; then preparing gelatin methylacrylate hydrogel loaded with modified teriparatide, compounding the gelatin methylacrylate hydrogel with a scaffold matrix, and carrying out blue light cross-linking curing to obtain a final product. The bone repair scaffold provided by the invention has good biocompatibility and osteogenic activity, and can effectively promote bone defect repair. Besides, the preparation method is controllable in process and good in repeatability, precise regulation and control of the scaffold structure are achieved through the 3D printing technology, and a new way is provided for preparation of personalized bone repair materials.