19 results about "Biphasic calcium phosphate" patented technology

The application provides a kind of load exosome composite hydrogel and preparation method thereof, belong to biomaterial and drug delivery technical field;Its preparation method includes: modified chitosan;Modified hyaluronic acid;Preparation of biphasic calcium phosphate nanoparticles;Preparation of composite hydrogel.The application is through nucleophilic ring-opening reaction of 1,2-epoxy butane and the hydroxyl group of chitosan, form hydroxybutyl ether bond, give hydroxybutyl chitosan temperature sensitive characteristics, then, again, after activation 3,4-dihydroxyphenylalanine is condensed with hydroxybutyl chitosan, form amide bond, to graft 3,4-dihydroxyphenylalanine on hydroxybutyl chitosan, after modification of chitosan, 3,4-dihydroxyphenylalanine can form strong adhesion interface with cartilage, synovial membrane and other tissues through hydrogen bond, pi-pi stacking and metal coordination, thereby effectively prolonging the residence time of hydrogel in joint.

The invention discloses a cation-doped biphase calcium phosphate material and a preparation method and application thereof.The preparation method comprises the steps that a chemical precipitation method is adopted, the reaction process is precisely regulated and controlled, firstly, a reaction is carried out in an acidic microenvironment with the pH being 2-6, then aging is carried out under the alkaline condition with the pH being 9-12, and a two-step pH precise control strategy that acidity is carried out first and then alkalinity is carried out is adopted, so that the cation-doped biphase calcium phosphate material is obtained. According to the strategy, thermodynamic interference of cation doping on the HA / beta-TCP phase proportion can be effectively counteracted, and stable control over product phase composition is achieved. The preparation method has the core advantages that the proportion of hydroxyapatite (HA) to beta-tricalcium phosphate (beta-TCP) in the prepared biphase calcium phosphate material is highly stable, and the proportion does not obviously fluctuate in a specific range of the cation doping amount due to the change of the doping amount or cation types; the problem that the phase proportion is uncontrollable due to doping in the prior art is solved.

The invention relates to a preparation method of a porous silicon nitride interbody fusion cage based on photocuring 3D printing, the interbody fusion cage comprises a support body and a filling body, the support body is used for providing support, a part of the surface of the support body is provided with a hollow area, an accommodating space is formed in the support body, and the filling body is arranged in the accommodating space; the support body is made of a silicon nitride material and is formed through photocuring 3D printing; the filling body is made of biphase calcium phosphate ceramic and is formed through photocuring 3D printing; the filling body is of a porous structure. The support body and the filling body can be directly matched and play roles respectively, so that the connection reliability and higher precision are ensured, and the external silicon nitride bracket can provide enough strength in the dissolution and metabolism process of the filling body. The technical problems that an existing interbody fusion cage is unstable in connecting structure, prone to displacement and poor in mechanical property are solved.

The invention relates to a biphase calcium phosphate / hydroxyapatite bone substitute material and a preparation method thereof. The bone substitute material comprises a degradable calcium phosphate core layer, a porous hydroxyapatite precursor layer and a hydroxyapatite gradient layer, wherein the porous hydroxyapatite precursor layer is formed on the surface of the degradable calcium phosphate core layer in situ through a sol-gel method, and the hydroxyapatite gradient layer is formed through electro-deposition treatment and covers the porous hydroxyapatite precursor layer. According to the technical scheme, a layered mineralization structure with a porous hydroxyapatite precursor layer as a template is constructed, and a hydroxyapatite layer with a continuous gradient in the thickness direction is formed through segmented electro-deposition treatment, so that the obtained composite material realizes continuous transition from outside to inside in the aspects of porosity, grain size, crystallinity and Ca / P ratio. The structure of the biphase calcium phosphate / hydroxyapatite bone substitute material can remarkably improve the interface bonding strength, the ion exchange capacity and the biological activity, so that the osteoblast adhesion, proliferation and osteogenic differentiation capacities are improved, and the overall osteogenic performance is obviously superior to that of an existing material.

The application relates to the technical field of biomedical materials, and specifically discloses a preparation method of a double-crosslinked 3D-printed bone repair scaffold. The scaffold is made of a biphasic calcium phosphate matrix and a gelatin methacrylate hydrogel outer layer. The preparation method comprises the following steps: firstly, preparing biphasic calcium phosphate ink, and constructing a porous scaffold blank through 3D printing; then, carrying out double-crosslinking treatment of calcium chloride solution and glutaraldehyde solution in sequence to obtain a scaffold matrix with enhanced mechanical properties; then, preparing gelatin methacrylate hydrogel loaded with modified teriparatide, and compounding the gelatin methacrylate hydrogel with the scaffold matrix; and finally, obtaining the final product after blue light crosslinking and solidification. The bone repair scaffold has good biocompatibility and osteogenic activity, and can effectively promote bone defect repair. In addition, the preparation method is controllable and has good repeatability, the structure of the scaffold is accurately controlled through the 3D printing technology, and a new approach is provided for the preparation of personalized bone repair materials.

The invention discloses a shell-derived biphase calcium phosphate composite bone repair material as well as a preparation method and application thereof, and belongs to the field of biomedical materials. According to the preparation method, the shell-source calcium source and the calcium pyrophosphate are mixed, the biphase calcium phosphate (BCP) containing HAP and beta-TCP in different proportions is prepared through wet ball milling and solid-phase reaction, the reaction conditions are controlled in a simple mode, and the proportion of HAP to beta-TCP in the BCP is controllable. The biphase calcium phosphate prepared by the method is further compounded with sodium alginate, and the biphase calcium phosphate-sodium alginate composite scaffold is prepared by freeze drying and Cacrosslinking. The composite scaffold has a suitable porous structure, can exert in-vitro osteogenic induction activity and an in-vivo bone defect repair function, can significantly induce MC3T3-E1 cell alkaline phosphatase activity and induce calcium deposition, has osteogenic induction activity, can induce bone defect repair at an in-vivo rat skull defect part, and has a good application prospect. Excellent bone tissue engineering application prospects are shown.

The present invention discloses a degradable bone filling material, a preparation method and an application thereof, and belongs to the field of biomedical technology. The preparation method comprises: mixing a silk fibroin solution, a chitosan solution and a base gel, adding biphasic calcium phosphate, freezing and freeze-drying after mixing under heating conditions, taking out and soaking in water and placing it in a mold for freeze-drying to obtain a core skeleton structure; taking another silk fibroin solution, a chitosan solution containing methacryloylated RGD peptide and a base gel, mixing them, adding biphasic calcium phosphate, freezing and freeze-drying after mixing under heating conditions, covering the surface of the core skeleton structure, freezing and freeze-drying, taking out and soaking in water and freezing and freeze-drying again to obtain a porous skeleton structure; covering the surface of the porous skeleton structure with hydrogel, and obtaining. The present invention also discloses a degradable bone filling material and an application thereof. The degradable bone filling material prepared by the present invention has high mechanical strength, no biological toxicity and has the effect of promoting osteogenesis, and can be used to prepare bone tissue organoids and drugs or preparations for treating bone defects and skeletal system diseases.

This invention discloses a 3D-printed scaffold with improved bioactivity and in vitro mineralization capacity, its preparation method, and its applications. The scaffold is prepared by layer-by-layer extrusion 3D printing using an ink comprising biphasic calcium phosphate (composed of octacalcium phosphate (OCP) and amorphous calcium phosphate (ACP)), sodium alginate, and a dispersant. This invention uses a mixture of OCP and ACP to form biphasic calcium phosphate (BCP) as the inorganic phase component of the 3D composite scaffold, and sodium alginate (SA) as the organic phase component. Simultaneously, the organic substance sodium alginate (SA) can simulate a blood clot as a temporary matrix, providing mechanical stability and traction for cells migrating from adjacent tissues. Furthermore, it provides a stable environment for new bone replacement during the gradual degradation of the scaffold by cells, offering significant advantages for subsequent scaffold implantation, attracting cell migration, and inducing mineralization.

The preparation method comprises the following steps: mixing strontium carbonate, zinc oxide, calcium carbonate and calcium hydrophosphate, carrying out ball milling to obtain powder slurry, drying, sintering, and carrying out wet ball milling to obtain strontium / zinc co-doped beta-TCP powder; the preparation method comprises the following steps: mixing calcium hydrophosphate with calcium carbonate, ball-milling into powder slurry, drying, sintering, crushing and grinding to obtain tetracalcium phosphate powder; and carrying out three-dimensional mixing on the strontium / zinc co-doped beta-TCP powder, calcium hydrophosphate and tetracalcium phosphate to obtain the strontium-zinc co-doped biphase calcium phosphate bone cement powder. According to the invention, through collaborative design of composition and structure, multifunctional active regulation and control of the bone repair process are realized.

The utility model discloses a skull repairing mesh plate, and relates to the technical field of medical implants, the skull repairing mesh plate comprises a mesh plate main body, the mesh plate main body has a curved surface shape matched with a skull defect area; the screen plate main body is made of a composite material of polyether-ether-ketone and biphase calcium phosphate; the mesh plate main body comprises a compact area and a loose area, and the porosity of the compact area is smaller than that of the loose area; every two adjacent loose areas are separated through the corresponding dense areas, and the dense areas are staggered to form a net-shaped structure. First micropores are formed in the surface of the loose area; first through holes are formed in the compact area at intervals, and the first through holes are formed in the thickness direction of the screen plate body in a penetrating mode. The skull repairing screen plate has good mechanical properties; the bone integration capability is good; due to the lightweight design, foreign body sensation after implantation is reduced; the occurrence rate of subcutaneous hydrops can be reduced.

The invention belongs to the field of medical materials. The preparation method comprises the following steps: S1, preparing a K2HPO4 solution with the pH (Potential of Hydrogen) of 8 to 12 and the concentration of 0.25 M or a K2HPO4 solution with the pH of 7 to 12 and the concentration of 0.1 M; s2, Li-Ca-B glass microspheres are prepared, the Li-Ca-B glass microspheres and the phosphate solution in the step S1 are mixed, the mixture is heated to react for 2-4 days, and calcium-deficient hydroxyapatite hollow microspheres are obtained; s3, washing and drying the calcium-deficient hydroxyapatite hollow microspheres; and S4, calcining the dried calcium-deficient hydroxyapatite hollow microspheres to obtain the biphase calcium phosphate hollow microspheres. According to the preparation method disclosed by the invention, the biphase calcium phosphate hollow microspheres with different proportions of HA / beta-TCP can be prepared, the dynamic balance of material performance is realized, and meanwhile, the prepared biphase calcium phosphate hollow microspheres have gradient pores and can promote the repair of bone tissues.

The invention provides exosome-loaded composite hydrogel and a preparation method thereof, and belongs to the technical field of biological materials and drug delivery. The preparation method comprises the following steps: modifying chitosan; modifying hyaluronic acid; preparing biphase calcium phosphate nanoparticles; and preparing the composite hydrogel. The preparation method comprises the following steps: carrying out a nucleophilic ring-opening reaction on 1, 2-epoxybutane and hydroxyl of chitosan to form a hydroxybutyl ether bond, endowing hydroxybutyl chitosan with a temperature-sensitive characteristic, carrying out a condensation reaction on hydroxybutyl chitosan and activated 3, 4-dihydroxyphenylalanine to form an amido bond, grafting 3, 4-dihydroxyphenylalanine onto hydroxybutyl chitosan, and carrying out a reaction on the 3, 4-dihydroxyphenylalanine and the hydroxybutyl chitosan to form the temperature-sensitive hydrogel. After chitosan is modified, 3, 4-dihydroxyphenylalanine can form a strong adhesion interface with tissues such as cartilage and synovial membranes through hydrogen bonds, pi-pi accumulation and metal coordination, so that the residence time of the hydrogel in joints is effectively prolonged.

The invention relates to a silicon nitride interbody fusion cage based on photocuring 3D printing and a preparation method, the interbody fusion cage comprises a support body and a filling body, the support body is used for providing support, a part of the surface of the support body is provided with a hollow area, an accommodating space is formed in the support body, and the filling body is arranged in the accommodating space; the support body is made of a silicon nitride material and is formed through photocuring 3D printing; the filling body is made of biphase calcium phosphate ceramic and is formed through photocuring 3D printing; the filling body is of a porous structure. The support body and the filling body can be directly matched and play roles respectively, so that the connection reliability and higher precision are ensured, and the external silicon nitride bracket can provide enough strength in the dissolution and metabolism process of the filling body. The technical problems that an existing interbody fusion cage is unstable in connecting structure, prone to displacement and poor in mechanical property are solved.

The present invention relates to a two-stage sintering method for preparing a porous biphasic calcium phosphate ceramic from calcium-containing biological waste, wherein hydroxyapatite prepared from calcium-containing waste is mixed with a foaming agent to prepare a bone graft material having medicinal use through two-stage sintering.

This invention discloses a calcium phosphate ceramic particle-methacrylated gelatin hydrogel scaffold for osteoblasts pretreated with Tideglusib to enhance osteogenic differentiation, relating to the fields of biomedical engineering and bone tissue regeneration. It comprises osteoblasts pretreated with Tideglusib and activated, biphasic calcium phosphate ceramic particles, and a methacrylated gelatin hydrogel encapsulating the osteoblasts and ceramic particles. The preparation method includes: obtaining rat osteoblasts; seeding the osteoblasts onto the biphasic calcium phosphate ceramic particles; and pretreating the seeded biphasic calcium phosphate ceramic particles with Tideglusib solution to activate the Wnt signaling pathway and promote osteogenic differentiation. This invention exhibits excellent bone defect repair capabilities, good degradability and biocompatibility, provides necessary mechanical support, and has strong clinical translational potential.

The invention belongs to the technical field of bone tissue repair, and particularly relates to a bone tissue engineering scaffold for critical dimension bone defect repair and a preparation method. Comprising the following steps: preparing a BCP hollow spherical structure scaffold, carrying out surface mineralization modification by using an in-situ mineralization technology, and screening a mineralization modification structure for modification design; constructing a three-dimensional delivery system of the BMSCs by taking GelMA as a carrier; and constructing the 3D printing BCP hollow spherical mineralization modification composite scaffold loaded with the BMSCs three-dimensional delivery system. According to the invention, a digital light treatment photopolymerization 3D printing technology is utilized, a biomimetic raw material biphase calcium phosphate is based on components, a hollow spherical structure modification strategy is innovated, an in-situ mineralization technology is utilized for surface modification, and bone marrow mesenchymal stem cells are loaded through a three-dimensional delivery system, so that a new idea is provided for BTE design, and a new thought is provided for clinical treatment of bone defects.

An efficient biphasic calcium phosphate coating method. A high-concentration (4.5×) supersaturated calcium phosphate solution (SCPS) is used for coating and a thicker continuous coating is formed on a titanium disc. The coating contains dicalcium phosphate dihydrate and hydroxyapatite. The coating has improved encapsulation efficiency of a protein FITC-BSA and releases less Ca2+ under an acidic condition. A more efficient coating with a high yield and a protein encapsulation efficiency can be obtained with a less SCPS solution volume and a shorter coating time, such that a cost is greatly reduced in clinical application and the coating is very expected to be used in an osteogenic implant material in the biomedical field.

The invention relates to the technical field of biomedical materials, and particularly discloses a preparation method of a double-crosslinking 3D printing bone repair scaffold. The scaffold is prepared by compounding a biphase calcium phosphate matrix and a gelatin methylacrylate hydrogel outer layer. The preparation method comprises the following steps: firstly, preparing biphase calcium phosphate ink, and constructing a porous scaffold blank through 3D printing; sequentially carrying out double crosslinking treatment on a calcium chloride solution and a glutaraldehyde solution to obtain a scaffold matrix with enhanced mechanical properties; then preparing gelatin methylacrylate hydrogel loaded with modified teriparatide, compounding the gelatin methylacrylate hydrogel with a scaffold matrix, and carrying out blue light cross-linking curing to obtain a final product. The bone repair scaffold provided by the invention has good biocompatibility and osteogenic activity, and can effectively promote bone defect repair. Besides, the preparation method is controllable in process and good in repeatability, precise regulation and control of the scaffold structure are achieved through the 3D printing technology, and a new way is provided for preparation of personalized bone repair materials.

The invention discloses a tooth filling material based on polyphenol and collagen modified magnesium-based powder / biphase calcium phosphate composite powder, and a preparation method comprises the following steps: dissolving a polyphenol compound and magnesium-based powder / biphase calcium phosphate composite powder in absolute ethyl alcohol, adding an alkaline solution, adjusting the solution to be alkaline, and stirring uniformly; stirring to obtain polyphenol modified magnesium-based powder / biphase calcium phosphate composite powder; dissolving collagen and the centrifuged product into deionized water, and adjusting to a proper concentration; stirring to immerse the polyphenol modified magnesium-based powder / biphase calcium phosphate composite powder into the collagen solution; and adding ammonium persulfate to agglomerate the collagen to obtain the magnesium-based powder / biphase calcium phosphate composite powder based on polyphenol and collagen modification. According to the tooth filling filler as well as the preparation method and the application thereof, a new solution is provided for the field of dental implantation.