Biphasic calcium phosphate porous bioceramic bone scaffold material and preparation method thereof and application

A biphasic calcium phosphate, bioceramic technology, applied in tissue regeneration, medical science, prosthesis and other directions, can solve the problems of single component, difficult to degrade biological activity, etc., and achieves low preparation cost, simple and feasible preparation method, and suitable porosity. Effect

Inactive Publication Date: 2017-12-12
SHANGHAI CHANGHAI HOSPITAL
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is that the present invention is aimed at the common heterogeneous natural bone scaffold materials with single components, HA is difficult to degrade and has poor biological activity, and provides a biphasic calcium phosphate porous bioceramic bone scaffold material, which has important clinical application value

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Biphasic calcium phosphate porous bioceramic bone scaffold material and preparation method thereof and application
  • Biphasic calcium phosphate porous bioceramic bone scaffold material and preparation method thereof and application
  • Biphasic calcium phosphate porous bioceramic bone scaffold material and preparation method thereof and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1, preparation of fishbone derived bone scaffold material

[0036] Raw material: salmon bone 100g, salmon, scientific name in English: Salmo salar, place of origin: Norway.

[0037] Removal of organic matter: Boil the fish bones in deionized water for 1 hour to remove visible organic matter, rinse with deionized water and boil again for 1 hour, repeat this step 3 times. Dry the cleaned fish bones in a constant temperature drying oven at 55°C for 24 hours to remove excess water.

[0038] High-temperature calcination: put the dried fish bones into a muffle furnace, control the heating rate at 5-10°C / min, control the high-temperature calcination temperature at 900°C, control the calcination time at 1 hour, and control the cooling rate at 10°C / min, and finally 47.8 g of calcined fish bones were obtained.

[0039] Grinding and sieving: the calcined bone was ground in an agate mortar, and sieved with a Taylor standard sieve of 35 mesh and 18 mesh wire mesh to obt...

Embodiment 2

[0042] Embodiment 2, composition, pore structure analysis and porosity determination of bone scaffold material

[0043] The bone scaffold material obtained in Example 1 is a white granular porous material, such as figure 2 shown. Using a scanning electron microscope to analyze its microstructure, it is found that the material has interconnected pore structures ranging in size from 50-300um, such as image 3 As shown, such a structure is conducive to cell ingrowth and nutrient transport. In order to understand the composition of the bone scaffold material, X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) were used to analyze the material.

[0044] The method for component analysis of the bone scaffold material in Example 1 by X-ray diffraction is as follows: the prepared granular bone scaffold material is repeatedly ground into a uniform fine powder in an agate mortar, pressed into tablets, tested on the machine, and set parameters It is a copper t...

Embodiment 3

[0048] Embodiment 3, the in vitro cytotoxicity experiment of bone scaffold material

[0049] According to the national standard GBT 16886.5-2003 method, the cytotoxicity was detected by using the material extract. The specific method is as follows:

[0050] Preparation of extract solution: add biphasic calcium phosphate porous bioceramic bone scaffold material (ratio of material mass to extraction medium capacity is 0.1g / mL) in DMEM medium containing 10% FBS and double antibody, at 37°C Continue soaking in a constant temperature shaker for 24 hours, then sterilize with a 0.22um filter, seal in a sterile bottle, and store in a refrigerator at 4°C for later use.

[0051] The CCK-8 method is used to detect the cytotoxicity of the material: take a 96-well plate, add 3000 cells to each well, and pre-culture for 24 hours. The DMEM medium of the double antibody was put into the incubator again for 1, 3, and 5 days. Before the test, add 10uL CCK-8 reagent to each well and incubate ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
porosityaaaaaaaaaa
Login to view more

Abstract

The invention relates to the field of bioceramic materials, in particular to a biphasic calcium phosphate porous bioceramic bone scaffold material which is prepared from salmon spine as a raw material, is composed of main ingredients hydroxyapatite and beta-tricalcium phosphate , has high porosity, reserves a natural three-dimensional pore structure, has a particle size of 500-1000 [mu]m, and is prepared through steps of washing and drying, high temperature calcination, grinding, sieving and sterilization. The biphasic calcium phosphate porous bioceramic bone scaffold material is rich in sources of raw materials and low in preparation cost, high purity biphasic calcium phosphate ceramic can be prepared without artificial addition of compounds, and the content of hydroxyapatite and beta-tricalcium phosphate can be changed by controlling the calcination temperature in the preparation process, and the biphasic calcium phosphate porous bioceramic bone scaffold material contains a small amount of carbonated hydroxyapatite and various trace elements such as magnesium, potassium and strontium, with the content close to the content in the human body. The calcium phosphate ceramic prepared through the method can serve as a good bone scaffold material to be applied to clinical bone defect repair.

Description

technical field [0001] The invention relates to the fields of bioceramic materials and biomedical materials, in particular to a biphasic calcium phosphate porous bioceramic bone scaffold material and its preparation method and application. Background technique [0002] Bone defect is a common clinical disease. In orthopedics, large-scale bone defects caused by severe trauma, infection, tumor resection and various congenital diseases often require surgeons to repair them through bone grafting. In the Department of Stomatology, the alveolar bone defect caused by patients with long-term tooth loss, periodontitis, osteoporosis or tooth extraction has also become a major problem in dental implant technology. Bone graft surgery to increase the bone mass of the alveolar bone. An ideal bone graft material not only needs to have good physical and chemical properties that can meet the needs of bone cell migration and growth, including suitable pore size, porosity and degradation rate...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/12A61L27/56A61L27/58
CPCA61L27/12A61L27/3608A61L27/365A61L27/3683A61L27/56A61L27/58A61L2430/02A61L2430/40
Inventor 王少海朱庆丰高建勇蔡齐祖丽皮也阿不力克木陈彤
Owner SHANGHAI CHANGHAI HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap