42 results about "Salmo" patented technology

The present invention uses fresh water as farming environment, according to the basic requirement for growth of salmon the farming water source, water quality condition, stocking density and bait matching proportion are defined to make culture, at the same time the fish pond, subsider, filtering pool, refrigeration equipment, water pump and water collecting tank are formed into a semi-closed circulation farming system, and in the circulation the problems of discharging sewage, filtering and disinfection, temp. regulation, water supply and aeration also can be resolved.

The present invention is directed to a simple method for absolute quantification of plasma vitellogenin from two or more different fish species such as Rainbow trout and Atlantic salmon, or Atlantic cod and haddock. In the case of Rainbow trout and Atlantic salmon, plasma samples obtained from control and β-estradiol induced fish were digested with trypsin. A characteristic ‘signature peptide’ was selected and analyzed by high performance liquid chromatography coupled to an electrospray quadrupole-time-of-flight tandem mass spectrometer, using a deuterated homologue peptide as an internal standard. The hybrid tandem mass spectrometer was operated in a ‘pseudo’ selected reaction monitoring mode by which three diagnostic product ions were monitored for identification and quantification purposes. The reproducibility (coefficient of variation ˜5%) and sensitivity (limit of quantification of 0.009 mg / mL) achieved by this simple assay allow it to be considered as an alternative to immunological assays. In the case of Atlantic cod and haddock, the amino acid sequence of the vitellogenin protein has not yet been determined, but, the Atlantic cod vitellogenin has been characterized using a ‘bottom-up’ mass spectrometric approach. Vitellogenin synthesis was induced ‘in vivo’ with β-Estradiol, and subjected to trypsin digestion for characterization by matrix-assisted laser desorption / ionization-Quadrupole-Time-of-flight tandem mass spectrometry. A peptide mass fingerprint was obtained and ‘de novo’ sequencing of the most abundant tryptic peptides was performed by low energy collision induced dissociation-tandem mass spectrometry. Thus, the sequences of various tryptic peptides have been elucidated. It has also been determined that Atlantic cod vitellogenin shares a series of common peptides with the two different known vitellogenin sequences of Haddock, a closely related species. There are also disclosed novel isolated signature peptides, namely Thr-Tyr-Phe-Ala-Gly-Ala-Ala-Ala-Asp-Val-Leu-Glu-Val-Gly-Val-Arg, Asp Leu Gly Leu Ala Tyr Thr Glu Lys, Phe Phe Gly Gln Glu Ile Ala Asn Ile Asp Lys, Glu Ile Val Leu Leu Gly Tyr Gly Thr Met Ile Ser Lys and Tyr Glu Ser Phe Ala Val Ala Arg.

A cosmeceutically acceptable formulation as a wound dressing including red palm oil and a triglyceride, preferably fish oil, preferably derived from salmon. Another aspect of this disclosure is providing a mixture of red palm oil and very long chain (>C>18) omega 3 and 6 fatty acids. The formulation may also include one or more of the following: coconut oil and shea butter for anti-inflammatory activity and antimicrobial efficacy, fish collagen, an acidic preservative, a cationic surfactant, and various vitamins.

The present application disclosed a method for preparing a sterile transgenic fish, comprising constructing antisense RNA expression vector of salmon-type gonadotropin-releasing hormone, introducing the recombinant DNA fragment into carp oosperm by microinjection, and screening the sterile transgenic fish by Polymerase Chain Reaction and radioimmunoassay, wherein the expression vector comprising a promoter of carp beta actin (β-actin) gene, a complementary DNA fragment of antisense salmon-type gonadotropin-releasing hormone (sGnRH) gene from carp with 323 bp as a target gene comprising sGnRH decapeptide, the coding region of gonadotropin-releasing hormone associated peptide and 3′ non-coding sequence, and 3′ flanking sequence of grass carp growth hormone gene as a stop sequence. The method of the present invention is easy and convenient for operation which provides a basis for providing a technical platform of general applicability for solving the hereditary and ecological safety problems of transgenic fishes.

Chicken II and salmon GnRH analog decapeptides resistant to degradation by peptidase incorporating D-arginine, D-leucine, D-tBu-Serine, D-Trp or other active D amino acids at position 6 and ethylamide, aza-Gly-amide or other Gly amide at position 10. The analogs demonstrate preferential binding to male and female reproductive system GnRH receptors. Biopotency is greater within the reproductive system than at the pituitary. These non-mammalian GnRH analogs may be used in pharmaceutical preparations, and specifically in various treatment methods as a contraceptive or post-coital contraceptive agent. The non-mammalian GnRH analogs are also provided in pharmaceutical preparations that may be used clinically for maintaining pregnancy when used in very low doses and administered in pulsatile fashion, as well as in preparations for the treatment of male and female reproductive system disorders. The aza-Gly (10) amide non-mammalian analogs are yet other embodiments of the non-mammalian GnRH analogs provided as a part of the invention.

The present invention pertains to a method for automatic sea lice monitoring in fish aquaculture, the method comprising submerging a camera (4) in a sea pen (300) comprising fish, using the camera to make an image of at least one of said fish, analysing the image to differentiate between individual sea lice present on the fish and the fish itself and assessing the number of sea lice present on the fish, wherein the camera is attached to a device (1, 10, 100) for guiding the salmon along an imaging track (5), the camera being directed to the track.

The invention discloses a Danio rerio B-lymphocyte activating factor (zBAFF) cDNA (complementary deoxyribonucleic acid), and a cloning method and application thereof in recombination, and relates to the field of biological gene engineering. The cloning method of the zBAFF gene comprises the following steps: extracting total RNA (ribose nucleic acid) of the spleen of Danio rerio by a TRIzol method, and carrying out reverse transcription; and analyzing BAFF cDNAs of rainbow trout, Atlantic salmon and Tetraodon nigroviridis, and conversed regions of BAFF cDNAs of rainbow trout, Atlantic salmon and Ictalurus punctatus by Clustalw software, designing degenerate primers, and carrying out PCR (polymerase chain reaction) amplification to obtain the 807bp full-length cDNA. The Danio rerio B-lymphocyte activating factor and proliferation inducing ligand cDNA can be used for producing recombinant Danio rerio BAFF by the existing gene engineering method.

Chicken II and salmon GnRH or its analog decapeptides resistant to degradation by peptidase incorporating D-arginine, D-leucine, D-tBu-Serine, D-Trp or other active D amino acids at position 6 and ethylamide, aza-Gly-amide or other Gly amide at position 10. The non-mammalian GnRH or its analogs demonstrate preferential binding to male and female reproductive system GnRH receptors as well as tumor cell GnRH receptors in these systems. Biopotency is greater within the reproductive system and at tumor cells than at the pituitary. These non-mammalian GnRH or its analogs may be used in pharmaceutical preparations, and specifically in various treatment methods as a contraceptive or post-coital contraceptive agent. The non-mammalian GnRH or its analogs are also provided in pharmaceutical preparations that may be used clinically for maintaining pregnancy when used in very low doses and administered in pulsatile fashion, as well as in preparations for the treatment of male and female reproductive system disorders including cancers of these systems or other system with GnRH II receptors. The aza-Gly (10) amide non-mammalian analogs are yet other embodiments of the non-mammalian GnRH analogs provided as a part of the invention.

A process for harvesting fish eggs is provided, in particular fish eggs from Salmon. The process comprises rearing sexually immature salmon in an aquatic environment in stages during which at least the light exposure and time span is adjusted. The rearing includes at least a winter-summer period that comprises a winter life-cycle stage, within which the broodstock is exposed to light that simulates winter light exposure, and a subsequent summer life-cycle stage, within which the broodstock is exposed to light that simulates summer light exposure, wherein the total Accumulated Thermal Unit (ATU) during the winter-summer period is no more than 5000. The inventions also provides salmon eggs that are produced by the disclosed process.

A method of alleviating central nervous system-mediated pain includes applying salmon thrombin at a neural injury site. Applying salmon thrombin can include applying a gel that includes salmon thrombin. The gel can also include fibrinogen, for example, salmon fibrinogen, human fibrinogen, or bovine fibrinogen. The salmon thrombin can be obtained from salmon plasma, or using recombinant technology, or by fractionation. A pain relief substance includes a gel that includes salmon thrombin.

Nucleic acid encoding recombinant salmon calcitonin, expression vector thereof, and method for producing recombinant salmon calcitonin therewith. The nucleic acid encoding recombinant salmon calcitonin comprises the sequence of SEQ ID No. 2

The invention belongs to the technical field of aquaculture, and relates to a catadromous migration salmon breeding method for migratory salmon. The method specifically comprises the following steps:(1) selection of juvenile salmons, wherein the average sampling weight is at least 25g, and the proportion of individuals smaller than 25g is not higher than 10%; (2) during the catadromous migrationperiod, adding an underwater light source at the bottom of the culture pond to ensure that the illumination intensity in the water body at the bottom of the pond is not lower than 20Lux; (3) cultivation in a catadromous migration stage, wherein a light control strategy is as follows: firstly, a light cycle strategy (LD12: 12) of 12 hours of illumination every day and 12 hours of darkness is adopted for 6 weeks, and then the light cycle strategy is converted into 24 hours of illumination every day (LD24: 0) and lasts for 6 weeks as well; and (4) completing the catadromous migration stage. According to the method, the weight of the catadromous migration juvenile salmon is controlled, light rays are controlled, and the form and physiological indexes of the catadromous migration stage are monitored. The catadromous migration rate can be increased by at least 4.9%, the survival rate after s catadromous migration is increased by at least 6.3%, and large-scale cultivation of catadromous migration salmons under the industrialized condition can be achieved.

The invention discloses a method for rapidly identifying Atlantic salmon and rainbow trout based on a mass spectrometry technology. The method comprises the steps of (1) preparing an Atlantic salmon sample and a rainbow trout sample; (2) cooling the two fish meat samples to obtain samples; (3) obtaining a filtrate sample; (4) taking the sample obtained in the step (3), drying, and analyzing in a mass spectrometer; (5) acquiring two kinds of mass spectrum data after mass spectrum analysis in the step (4); (6) purchasing the Atlantic salmon on the market, processing the Atlantic salmon accordingto the methods in the steps (1) to (4), and then collecting mass spectrum data; and (8) comparing similarity scores of the mass spectra of the purchased Atlantic salmon in the step (6) and the rainbow trout sample in the step (5). According to the invention, the Atlantic salmon and rainbow trout are distinguished and identified by using a matrix-assisted laser desorption / ionization mass spectrometry technology, so that a high-efficiency, time-saving, rapid, sensitive and high-flux detection effect is achieved.

A salmon's calcitonin gene synthesized by plant preference codon, its fusion gene configurator, the recombinant expression carrier carrying said configurator, the plant cell converted by said expression carrier, the transgenic plant and its descendant generated by said converted cells for expressing said salmon's calcitonin, and the process for detecting the nucleic acid sequence and polypeptide of salmon's calcitonin in specimen are disclosed.

A seaweed-based commercial salmon feed additive which replaces the synthetic chemical additives that are currently used in salmon fish feed is provided. Synthetic additives are replaced with a combination of seaweed species thereby providing a natural product that improves the nutritional value of the farmed fish, qualifies the fish for marketing as organic, reduces the environmental impact of fish farming practices and may replace the chemical use of lice treatment.

The invention discloses a salmo trutta fario linnaeus parent fish feed with low protein and amino acid balance, which belongs to the technical field of aquatic feed products, and can be used for solving the problems that no salmo trutta fario linnaeus feed exists and the protein conversion rate is low and the amino acid is unbalanced when a trout feed is used. The salmo trutta fario linnaeus parent fish feed comprises wheat middling, soybean protein concentrate, fish meal, fish oil, soybean soil, cellulose, calcium dihydrogen phoshate, inkfish paste, soyabean lecithin, ethoxyquin, arginine, methionine, phenylalanine and a premix. According to the salmo trutta fario linnaeus parent fish feed, the utilization rate of a feed raw material with lower digestibility of salmo trutta fario linnaeus is reduced, the quality of the salmo trutta fario linnaeus parent fish feed is easily controlled, the digestion utilization rate of the salmo trutta fario linnaeus parent fish feed is improved, a raw material source is stable, the cost is lowered, the problem of unbalanced amino acid of a conventional feed is overcome, the protein efficiency of the feed is favorably improved, the emission of ammonia nitrogen is reduced, the pollution to a water environment is alleviated, the anti-stress ability and the immunity of a salmo trutta fario linnaeus parent fish are improved, and the quality of sperm eggs is improved.

A kit comprising an oligonucleotide-immobilized substrate obtained by immobilizing, on a substrate, one or more kinds of oligonucleotides that enable detection of a polymorphism in a nucleotide sequence of mitochondrial DNA control region of chum salmon, of which standard is represented by the nucleotide sequence of SEQ ID NO: 8, at a position selected from the 10th, 30th, 42nd, 57th, 70th, 96th, 108th, 154th, 194th, 231st, 242nd, 250th, 260th, 339th, 340th, 386th, 395th, 401st and 471st positions is used to detect the polymorphism based on hybridization of the oligonucleotides with a nucleic acid derived from specimen chum salmon and thereby determine a haplotype of the specimen chum salmon.

The present invention provides transgenic mice and nucleic acid constructs and methods for creating them. The transgenic mice are generated preferably by electroporation-mediated transfection of a nucleic acid construct into the testes of a sterile spermatogenesis-deficient male mouse. The construct includes DNA encoding a spermatogenesis rescue factor resulting in rescue of spermatogenesis while contemporaneously transferring a nucleic acid of interest for expression in the transgenic mouse so that progeny sired by the transgenic mouse also harbor the transgene. Certain embodiments of the invention also utilize (a) the salmonid derived Sleeping Beauty transposition system to augment genomic integration of the transgene and / or (b) the Cre / loxP recombination system for excision from sired progeny of the DNA responsible for spermatogenesis rescue.

The invention relates to a novel salmon calcitonin analogue and a preparation method thereof. Compared with a natural salmon calcitonin, the chemical stability of a compound provided by the invention is improved and the pharmacological activity of the natural salmon calcitonin is kept. The salmon calcitonin analogue provided by the invention has the following chemical structure.

The invention discloses an underwater salmon weight estimation method and system based on machine vision, and the method comprises the steps: obtaining a salmon picture data set, training the salmon picture data set through a deep learning algorithm, and obtaining a salmon recognition model; shooting an underwater image through a camera; identifying and marking the salmon in the underwater image by using a salmon identification model; obtaining an estimated distance from the camera to the salmon; calculating the estimated length of the salmon through the estimated distance; and obtaining the estimated weight of the salmon according to the estimated length. On the basis, the weight of the salmons can be estimated underwater at low cost, the camera equipment can freely move underwater, multiple salmons can be detected at a time, and the working efficiency is greatly improved.

A method and apparatus for filleting fish to produce high yielding fillets from relatively short bodied fish in the size range of about 600 grams to about 3.5 kilograms dependent on species. The method and apparatus are designed to be capable of handling salmon, tilapia, trout, arctic char, barramundi, snappers, walleye pike, striped bass and similar species where the rib follows the contour of the belly cavity. Further, the method and apparatus incorporate belly opening and eviscerating components prior to subjecting the fish to a backbone removal system so that the fish need only be headed, but not gutted, before introduction into the machine.

The invention discloses a formula and a preparation method of an extruded feed for spotted trout and salmon, aiming at providing a feed that is suitable for the digestive physiological characteristics of spotted trout and salmon, has good palatability, strong disease resistance, high nutrient digestibility, and can reduce the use of fish meal. amount, solve the problem of shortage of fishmeal resources, reduce the addition of inorganic phosphorus sources, and reduce the puffed feed of aquaculture water phosphorus pollution, so as to drive the development of spotted trout and salmon aquaculture in China; the present invention solves the problem of fishmeal content in puffed feed by optimizing the feed formula. The problem of too high improves the digestibility and palatability of the feed. The feed product of the invention can significantly promote the rapid growth of spotted trout and salmon, improve the survival rate of breeding, reduce the cost of breeding, improve the digestion and absorption rate of breeding, and reduce the excretion of nitrogen and phosphorus. Improve the breeding environment and other advantages.

The invention discloses a mass spectrometric identification method for Atlantic salmon and rainbow trout, comprising the following steps: a) obtaining the amino acid sequence of Atlantic salmon; b) obtaining the amino acid sequence of rainbow trout; c) using a specific enzymatic hydrolysis method to extract the protein to be detected Cut into small molecular weight polypeptide fragment mixtures, use electrostatic field orbitrap high-resolution mass spectrometry full-scan mode to detect the molecular weight and fragment information of each polypeptide in the mixture, and compare with the previously obtained amino acid sequences to qualitatively determine whether there are Atlantic salmon and rainbow trout d) Select the characteristic peptides qualitatively identified as Atlantic salmon or rainbow trout, and use tandem quadrupole mass spectrometry to establish a multiple reaction monitoring method for quantitative analysis. The invention utilizes the identification of protein characteristic peptides, can realize the qualitative identification and adulteration quantitative research of Atlantic salmon and rainbow trout at the same time, the minimum detection limit can reach 1%, high sensitivity, high accuracy, strong anti-interference ability, and can be widely used Authenticity of Atlantic salmon.

The present invention relates to a method, a system and an apparatus for treating food items. Such method and device may, for instance, be used to cool down food items, bleed freshly slaughtered fish and treat live fish against parasites. The device and method of the present invention further uses temperature differences and agitation to tread food items. A method for reducing or eliminating sea lice from salmon fish, said method comprising: a) transporting the salmon fish from a reservoir and the feeding salmon fish in liquid into the tubing of a first pumping device, said liquid being at a temperature from +15 to +28° C., b) transporting the salmon fish in liquid through the tubing of the pumping device at a first set time period for treatment in said liquid, c) feeding the salmon fish out of the first pumping device, d) separating the liquid from the salmon fish, and e) transporting the salmon fish back to the reservoir, wherein the liquid media from the pumping device is redirected after separation from the salmon fish in step d) through a heat exchange element back into the pumping device for continued treatment of further salmon fish, and wherein the temperature difference between the liquid used in steps b) and the reservoir is more than 15° C.

The invention relates to a salmon preservation method. The salmon preservation method comprises the following steps of: S1, selecting salmon: selecting high-quality Atlantic salmon as raw salmon; S2,storing and transporting the raw salmon: storing the raw salmon in a cold insulation box, and adding crushed ice or a crushed ice bag to cover the surface of the raw salmon; S3, performing treatment before cutting: taking the raw salmon in the step S2 out of the cold insulation box, cleaning the raw salmon to remove suspension on the surface of the raw salmon, performing scale removal treatment after cleaning, and washing the raw salmon while removing scales; S4, sterilizing the whole salmon: performing ozone sterilization on the salmon treated in the step S3; S5, cutting the whole salmon to remove salmon heads: removing the salmon heads of the sterilized salmon by using a head removing cutter, and slicing and stripping the salmon body by using a slicing cutter; S6, finely dividing salmonflesh: cutting the salmon body into salmon slices, salmon blocks, salmon sections or salmon fillets; S7, packaging: performing air-filled packaging or vacuum packaging on the salmon slices, the salmonblocks, the salmon sections or the salmon fillets; and S8, storing and transporting finished salmon: storing and transporting the packaged salmon slices, salmon blocks, salmon sections or salmon fillets at 0-4 DEG C.

The present invention encompasses four full length nucleic acid and amino acid sequences for PolyValent Cation-Sensing Receptors (PVCR) in Atlantic Salmon. These PVCR have been named SalmoKCaR#1, SalmoKCaR#2, SalmoKCaR#3, and SalmoKCaR#4. The present invention includes homologs thereof, antibodies thereto, and methods for assessing SalmoKCaR nucleic acid molecules and polypeptides. The present invention further includes plasmids, vectors, host cells containing the nucleic acid sequences of SalmoKCaR #1, 2 3 and / or 4.

The invention relates to the field of feed additives and specifically relates to a drug suitable for liver and gallbladder protection of Atlantic salmons and application of the drug. The drug comprises rheum officinale, golden cypress, coptidis rhizome, folium isatidis, radix glycyrrhizae, scutellaria baicalensis, tulip, radix isatidis and verbena officinalis. An extract of the drug is uniformly mixed with feed, and the feed is provisioned to the Atlantic salmons at eight clock once every day, so that livers and gallbladders of the Atlantic salmons can be effectively protected. The drug provided by the invention has the advantages that the product cost is low, the toxicity or side effect to the Atlantic salmons is little, residues are less, the livers and gallbladders of the Atlantic salmons can be effectively protected, and fatty liver is reduced, so that the drug is beneficial to popularization and application.

The present invention falls within the technical field of aquaculture, and specifically, the invention relates to a specific solution for preventing and treating bacterial diseases using a Lactococcus lactis lactic acid bacterium transformed to produce an interferon type II (IFN II) immunostimulating cytokine, particularly interferon gamma (IFNg or IFNγ). Said transformed bacterium has been deposited in the Chilean Microbial Genetic Resources Collection at INIA with accession number RGM 2416 dated 22 Oct. 2017. The invention is also related to an immunostimulating food for aquaculture species comprising the lactic acid bacterium transformed with IFN type II from salmonidae. Moreover, the invention relates to the method for preparing said probiotic food, the method for preparing said lactic acid bacterium expressing IFNg, in other words which produces a cytokine that stimulates the antibacterial immune response.

A process for a cell culture wherein the cells are grown on a substrate, the improvement comprising the step of growing the cells on collagen substrate with the collagen having been extracted from an animal selected from the group consisting of a marine mamal and a bony fish. Preferred embodiments include those wherein the collage is either from salmon or a seal.