Method for rapidly identifying Atlantic salmon and rainbow trout based on mass spectrometry technology

Abstract

The invention discloses a method for rapidly identifying Atlantic salmon and rainbow trout based on a mass spectrometry technology. The method comprises the steps of (1) preparing an Atlantic salmon sample and a rainbow trout sample; (2) cooling the two fish meat samples to obtain samples; (3) obtaining a filtrate sample; (4) taking the sample obtained in the step (3), drying, and analyzing in a mass spectrometer; (5) acquiring two kinds of mass spectrum data after mass spectrum analysis in the step (4); (6) purchasing the Atlantic salmon on the market, processing the Atlantic salmon accordingto the methods in the steps (1) to (4), and then collecting mass spectrum data; and (8) comparing similarity scores of the mass spectra of the purchased Atlantic salmon in the step (6) and the rainbow trout sample in the step (5). According to the invention, the Atlantic salmon and rainbow trout are distinguished and identified by using a matrix-assisted laser desorption / ionization mass spectrometry technology, so that a high-efficiency, time-saving, rapid, sensitive and high-flux detection effect is achieved.

Description

technical field [0001] The invention relates to the technical field of mass spectrometry, and proposes a method for rapidly identifying Atlantic salmon and rainbow trout based on mass spectrometry analysis technology. Background technique [0002] Aquaculture is one of the most important and fast-growing industries in the world. As a fast-growing industry, aquatic products contain many nutrients and physiologically active substances that are beneficial to human health, so people pay more attention to aquatic products, especially seafood. Demand is constantly increasing. According to Baidu Encyclopedia, there are more than 30 species of salmon (Oncorhynchus) with commercial value. At present, the most common are 2 kinds of trout (salmon trout, golden trout) and 4 kinds of salmon (Pacific salmon, Atlantic salmon, arctic whitefish). , coho salmon). [0003] my country often imports Pacific salmon and Atlantic salmon. Atlantic salmon (Salmo salar) is a seafood that can be eat...

AI Technical Summary

Problems solved by technology

However, the preprocessing of spectral data by the multivariate analysis technology currently relied on involves first-order derivatives, second-order derivatives, mean centering, multivariate scattering correction, standard normal variable transformation, and normalization, and the steps are relatively cumbersome.

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