Polyvalent cation-sensing receptor in atlantic salmon

a technology of atlantic salmon and cations, which is applied in the direction of animal/human proteins, peptides/protein ingredients, peptides, etc., can solve the problems of inability to determine from these experiments, and achieve the effects of reducing mortality, increasing growth, and efficient growth of atlantic salmon

Inactive Publication Date: 2007-02-08
HARRIS H WILLAM JR +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0017] The present invention allows for a number of advantages, including the ability to more efficiently grow Atlantic Salmon, and in particular, transfer them to seawater with increased growth and reduce mortality. The technology of the present invention also allows for assaying or testing these salmon to determine if they are ready for transfer to seawater, so that they can be transferred at the best time. The technology of the present invention provides for the imprinting of salmon with an odorant so that the salmon, once imprinted, can later more easily recognize and / or distinguish the odorant. For example, an attractant that has been used to imprint salmon can be added to feed so that the salmon will consume more feed and grow at a faster rate. A number of additional advantages for the present invention exist and are apparent from the description provided herein.

Problems solved by technology

Since these analyses shown in FIG. 20 use PVCR degenerate primers, it is not possible to determine from these experiments whether the alterations in steady state PVCR mRNA content are the result of changes in individual SalmoKCaRs #1-4.

Method used

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Examples

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example 1

Molecular Cloning of Shark Kidney Calcium RECEPTOR RELATED PROTEIN (SKCaR)

[0171] A shark λZAP cDNA library was manufactured using standard commercially available reagents with cDNA synthesized from poly A+ RNA isolated from shark kidney tissue as described and published in Siner et al. Am. J. Physiol. 270:C372-C381, 1996. The shark cDNA library was plated and resulting phage plaques screened using a 32P-labeled full length rat kidney CaR (RaKCaR) cDNA probe under intermediate stringency conditions (0.5×SSC, 0.1% SDS, 50° C.). Individual positive plaques were identified by autoradiography, isolated and rescued using phagemid infections to transfer cDNA to KS Bluescript vector. The complete nucleotide sequence, FIG. 1, (SEQ ID NO: 1) of the 4.1 kb shark kidney PVCR related protein (SKCaR) clone was obtained using commercially available automated sequencing service that performs nucleotide sequencing using the dideoxy chain termination technique. The deduced amino acid sequence (SEQ I...

example 2

Expression / Activation Studies of SKCaR in Human Embryonic Kidney (HEK) Cells

[0172] PVCRs serve as salinity sensors in fish. These receptors are localized to the apical membranes of various cells within the fish's body (e.g., in the gills, intestine, kidney) that are known to be responsible for osmoregulation. A full-length cation receptor (CaR, also referred to as “PVCR”) from the dogfish shark has been expressed in human HEK cells. This receptor was shown to respond to alterations in ionic compositions of NaCl, Ca2+ and Mg2+ in extracellular fluid bathing the HEK cells. The ionic concentrations encompassed the range which includes the transition from freshwater to seawater. Expression of PVCR mRNA is also increased in fish after their transfer from freshwater to seawater, and is modulated by PVCR agonists. Partial genomic clones of PVCRs have also been isolated from other fish species, including winter and summer flounder and lumpfish, by using nucleic acid amplification with dege...

example 3

Defining Salinity Limits as an Assay to Identify Fish With Enhanced Salinity Responsive and Altered PVCR Function

[0184] Both anadromous fish (Atlantic salmon, trout and Arctic char) and euryhaline fish (flounders, alewives, eels) traverse from freshwater to seawater environments and back again as part of their lifecycles in the natural environment. To successful accomplish this result; both types of fish have to undergo similar physiological changes including alterations in their urine output, altering water intake and water absorption. In some cases, naturally occurring mutations to PVCR would provide for altered salinity adaptation capabilities that would have significant value for both commercial and environmental restoration uses. For example, identification of selective traits associated with PVCR mediated salinity responses might allow identification of new strains of fish for commercial aquaculture. Similarly, identification of selected environmental parameters from a host o...

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Abstract

The present invention encompasses four full length nucleic acid and amino acid sequences for PolyValent Cation-Sensing Receptors (PVCR) in Atlantic Salmon. These PVCR have been named SalmoKCaR#1, SalmoKCaR#2, SalmoKCaR#3, and SalmoKCaR#4. The present invention includes homologs thereof, antibodies thereto, and methods for assessing SalmoKCaR nucleic acid molecules and polypeptides. The present invention further includes plasmids, vectors, host cells containing the nucleic acid sequences of SalmoKCaR #1, 2 3 and / or 4.

Description

RELATED APPLICATIONS [0001] This application is a Divisional of U.S. application Ser. No. 10 / 410,885, filed Apr. 9, 2003, which is a continuation-in-part of U.S. application Ser. No. 10 / 125,778, filed Apr. 18, 2002; U.S. application Ser. No. 10 / 125,772, filed Apr. 18, 2002; and U.S. application Ser. No. 10 / 125,792, all filed Apr. 18, 2002; which are continuations-in-part of U.S. application Ser. No. 10 / 121,441, filed Apr. 11, 2002; which is a continuation-in-part and claims priority to International Application No. PCT / US01 / 31704, which designated the United States and was filed on Oct. 11, 2001, published in English, which claims the benefit of U.S. Provisional Application No. 60 / 240,392, filed on Oct. 12, 2000, and U.S. Provisional Application No. 60 / 240,003, filed on Oct. 12, 2000. The entire teachings of the above applications are incorporated herein by reference.BACKGROUND OF THE INVENTION [0002] In nature, anadromous fish like salmon live most of their adulthood in seawater, b...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12Q1/68C07H21/04A61K38/00C07K14/705C12N15/12
CPCA01K2227/40A61K38/00C12Q1/6888C07K2319/00C07K14/705C12Q2600/136C12Q2600/158
Inventor HARRIS, H. WILLAM JR.NEARING, JACQUELINEBETKA, MARLIES
Owner HARRIS H WILLAM JR
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