The invention provides a method and a
system for improving algal oil yield based on
circadian rhythm light regulation and control, and belongs to the technical field of algal
oil production, and the method comprises the following steps: a first illumination program: the ratio of light to dark of an illumination period is (12: 12)-(20: 4); after the concentration of the indication nutritional factors in the culture medium is consumed to a set threshold value, switching to a second illumination program: the ratio of brightness to
darkness of an illumination period is (6: 18)-(10: 14), and carrying out illumination by adopting ultra-high-light-intensity pulsed light and medium-low-light-intensity
recovery light alternately and circularly; and after the content of the
grease in the culture medium enters the
plateau stage, switching to a third illumination procedure: the ratio of light to dark in the illumination period is (4: 20)-(8: 16), culturing for 12-48 hours, and then harvesting the
grease. The invention provides a multi-stage dynamic light regulation and control strategy matched with the growth stage and the internal
circadian rhythm of microorganisms, efficient cooperation of
biomass accumulation and
grease synthesis is realized, the tradeoff relation between growth and grease accumulation in the traditional technology is finally broken through, and the illumination
energy consumption is greatly reduced.