The invention discloses a method for detecting fungi carried by rice seeds by using an improved rose Bengal culture medium. The method comprises the steps of culturing and detecting the rice seeds by using the improved rose Bengal culture medium, wherein the culturing comprises the steps of putting culture dishes in an incubator, carrying out culturing for 1 day under the conditions of 25 DEG C, 9,000Lx illumination intensity, 12h illumination and 12h darkness, putting the culture dishes in a refrigerator with the temperature of -20 DEG C after the seeds are imbibed, keeping the temperature for 12 hours, taking out the culture dishes, carrying out culturing for 6 days under the conditions that the constant temperature is 28 DEG C, the illumination intensity is 9,000Lx and the light cycle is 12h light period and 12h dark period, picking the fungi, and microscopically observing and checking species of the fungi carried by the seeds. According to the improved rose Bengal culture medium, copper sulfate, zinc nitrate and cadaverine are added, and the consumption of magnesium sulfate is lowered. The method disclosed by the invention is beneficial to the growth of heterogeneous fungi, the species of the fungi can be detected and separated, the operation is not prone to bacterial contamination, the diameter of fungal colonies is relatively small, and thus, the separation and counting of the fungi are facilitated.