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Method for inducing callus differentiation through peony stems

A callus and peony technology is applied in the field of inducing callus differentiation through peony stem segments, which can solve the problems of difficulty in differentiation and rooting, high browning mortality, pollution and the like, and achieve the effect of promoting callus differentiation.

Inactive Publication Date: 2018-11-20
JIYANG COLLEGE OF ZHEJIANG A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although there are many researches on the tissue culture of peony, there are still many problems to be solved: serious pollution, high mortality rate of browning, difficulty in differentiation and rooting, etc.
These problems have seriously hindered the commercial development of peony

Method used

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  • Method for inducing callus differentiation through peony stems

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specific Embodiment approach

[0009] The method for inducing callus differentiation by peony stem segment of the present invention, its preferred embodiment is:

[0010] Adopt the following differentiation medium to carry out callus differentiation of peony: add 6-BA3.0mg / L+NAA0.2mg / L, sucrose 30.0g / L, agar 7.0g / L in MS medium, the pH 5.6-5.8.

[0011] Specifically include the following steps:

[0012] The well-grown stem segment calli selected for proliferation are transferred into the differentiation medium for peony callus differentiation, the temperature is 25°C, the relative humidity is 70%, the photoperiod is 12h, and the light intensity is 35-40mol / m 2 / s, cultured for 30 days to observe the differentiation of callus.

[0013] The method for inducing callus differentiation through tree peony stems of the present invention can make the callus differentiation rate of tree peony stems reach 24.44%, which promotes callus differentiation to a large extent and lays a good foundation for the establishmen...

specific Embodiment

[0014] The present invention takes MS as the basic medium, adding:

[0015] 6-BA3.0mg / L

[0016] NAA0.2mg / L

[0017] Sucrose 30.0g / L

[0018] Agar 7.0g / L

[0019] Culture conditions: temperature 25°C, relative humidity 70%, photoperiod 12h, light intensity 35-40mol / m 2 / s, the culture time is 30d.

Embodiment 1

[0020] Embodiment 1: The influence of different plant hormones on stem callus differentiation

[0021] Materials and Methods

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Abstract

The invention discloses a method for inducing callus differentiation through peony stems. The method comprises the following steps: adding 3.0 mg / L of 6-BA, 0.2 mg / L of NAA, 30.0g / L of sucrose and 7.0g / L of agar into an MS culture medium, wherein the pH value is 5.6-5.8; transferring selectively proliferated stem callus having good growth into a differential medium for performing peony callus differentiation, wherein the temperature is 25 DEG C, the relative humidity is 70%, the light cycle is 12 hours, and the illumination intensity is 35-40 mol / m<2> / s; culturing for 30 days, and observing callus differentiation conditions. According to the method disclosed in the invention, the callus differentiation ratio of the peony stems can reach 24.44%, the callus differentiation is greatly promoted, and a good foundation is laid for establishing a peony regeneration system. The problem that the peony is difficult in differentiation during tissue culture is solved, and a technical support is provided for establishing the peony regeneration system.

Description

technical field [0001] The invention relates to a method for inducing callus differentiation through peony stem segments. Background technique [0002] Peony is a perennial deciduous shrub of Paeoniaceae Paeoniaceae. It is one of the top ten traditional famous flowers. It has good ornamental value and commercial value and is widely used in gardens. [0003] The traditional breeding method of peonies has a long cycle, low efficiency, and the quality of emergence varies, which is difficult to meet the needs of production. Using tissue culture method, the cycle is short, the efficiency is high, and it is suitable for large-scale production. At present, although there are many studies on tissue culture of peony, there are still many problems to be solved: serious pollution, high mortality rate of browning, and difficulties in differentiation and rooting. These problems have seriously hindered the commercial development of peonies. Contents of the invention [0004] The purp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 朱向涛金松恒季雯洪尔蔓程雨飞
Owner JIYANG COLLEGE OF ZHEJIANG A & F UNIV
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