Annual culturing method for marine medaka in lab

Abstract

The invention discloses an annual culturing method for marine medaka in a lab. Culturing conditions include: culture seawater is 28-30 in salinity and 4-8mg / L in dissolved oxygen level after subjected to five-stage filtering via a nylon film, biological adsorbent, activated carbon, biochemical filter and ultraviolet sterilization system; the culture water is at 26-30 DEG C; the culture density reaches 0.2-0.5L per fish in the growth period and reaches 0.5-1L per fish in the breeding period; a light cycle includes 14 hours of illumination and 10 hours of no illumination, and illumination is performed from 5:00 to 19:00; the culture male-to-female ratio is 2:3; in terms of feeding frequency and feeding time, the fishes are fed with marine tropical fish feed three times a day, from 7:30 to 8:30, from 11:30 to 12:30 and from 15:30 to 16:30, and are fed with Artemia larvae twice a day, from 9:45 to 10:15 and from 13:45 to 14:15. By the annual culturing method, the defects that breeding conditions are difficult to control, cost is high, continuous egg laying fails, egg laying amount is low, breeding conditions are limited, and the like are overcome.

Description

technical field [0001] The invention relates to a method for breeding fish, in particular to a laboratory annual breeding method for medaka in seawater (also known as medaka with black spot). Background technique [0002] At present, there is a lack of internationally recognized marine model fish species for marine ecotoxicology research. Black-spotted medaka ( Oryzias melastigma ) are ubiquitous in the seas of China, North Korea, Japan, and India, etc., with a short generation cycle, small genome, high spawning rate, easy observation, and wide range of salinity adaptation, and have the potential to become marine model organisms. At present, relevant scholars and institutions in China, Japan, Taiwan and South Korea have carried out a number of ecotoxicological studies and applications on it as a new test fish species. [0003] The black-spotted medaka has a white body, a flat side and a short back, and a pair of large anal fins at the back of the body. The eyes are large ...

AI Technical Summary

Problems solved by technology

[0006] The present invention aims to solve the above-mentioned deficiencies in the prior art, and proposes a method that can realize large-scale cultivation of black-spotted medaka adult fish in the laboratory, so as to solve the problems of difficult control of breeding conditions, high cost, inability to continue spawning, and laying eggs. Seawater medaka (black-spotted medaka, Oryzias melastigma ) Laboratory Anniversary Breeding Method