Annual culturing method for marine medaka in lab

A breeding method and laboratory technology, which is applied in the field of laboratory annual breeding of seawater medaka (also known as black-spotted medaka), can solve the problems of limited breeding conditions, inability to continue spawning, difficult breeding conditions, etc., and reach the market Good prospects and scientific research significance, good for growth and reproduction, and the effect of simplifying the breeding process

Active Publication Date: 2012-07-18
NATIONAL MARINE ENVIRONMENTAL MONITORING CENTRE
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Problems solved by technology

[0006] The present invention aims to solve the above-mentioned deficiencies in the prior art, and proposes a method that can realize large-scale cultivation of black-spotted medaka adult fish in the laboratory, so as to so

Method used

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Embodiment Construction

[0016] Specific embodiments of the present invention will be described in detail below.

[0017] A species of seawater medaka (Black-spotted medaka, Oryzias melastigma ) laboratory annual cultivation method, characterized in that: the cultivation conditions of the cultivation method are as follows:

[0018] Breeding water: the seawater after five-stage filtration of nylon membrane, biosorbent, activated carbon, biochemical filter and ultraviolet sterilization system, the salinity is controlled at 28-30, and the dissolved oxygen level is 4-8mg / L.

[0019] Breeding water temperature: 26-30 degrees Celsius,

[0020] Breeding density: the growth period is 0.2-0.5L / tail, and the black-spotted medaka that has grown for more than three months enters the breeding period, and the breeding density during the breeding period is 0.5-1L / tail.

[0021] Photoperiod: 14 hours of light, 10 hours of darkness, and the lighting time is 5:00-19:00

[0022] Breeding sex ratio: male: female = 2:3...

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Abstract

The invention discloses an annual culturing method for marine medaka in a lab. Culturing conditions include: culture seawater is 28-30 in salinity and 4-8mg/L in dissolved oxygen level after subjected to five-stage filtering via a nylon film, biological adsorbent, activated carbon, biochemical filter and ultraviolet sterilization system; the culture water is at 26-30 DEG C; the culture density reaches 0.2-0.5L per fish in the growth period and reaches 0.5-1L per fish in the breeding period; a light cycle includes 14 hours of illumination and 10 hours of no illumination, and illumination is performed from 5:00 to 19:00; the culture male-to-female ratio is 2:3; in terms of feeding frequency and feeding time, the fishes are fed with marine tropical fish feed three times a day, from 7:30 to 8:30, from 11:30 to 12:30 and from 15:30 to 16:30, and are fed with Artemia larvae twice a day, from 9:45 to 10:15 and from 13:45 to 14:15. By the annual culturing method, the defects that breeding conditions are difficult to control, cost is high, continuous egg laying fails, egg laying amount is low, breeding conditions are limited, and the like are overcome.

Description

technical field [0001] The invention relates to a method for breeding fish, in particular to a laboratory annual breeding method for medaka in seawater (also known as medaka with black spot). Background technique [0002] At present, there is a lack of internationally recognized marine model fish species for marine ecotoxicology research. Black-spotted medaka ( Oryzias melastigma ) are ubiquitous in the seas of China, North Korea, Japan, and India, etc., with a short generation cycle, small genome, high spawning rate, easy observation, and wide range of salinity adaptation, and have the potential to become marine model organisms. At present, relevant scholars and institutions in China, Japan, Taiwan and South Korea have carried out a number of ecotoxicological studies and applications on it as a new test fish species. [0003] The black-spotted medaka has a white body, a flat side and a short back, and a pair of large anal fins at the back of the body. The eyes are large ...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 穆景利靳非王菊英张志锋王新红
Owner NATIONAL MARINE ENVIRONMENTAL MONITORING CENTRE
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