63 results about "Harmonia axyridis" patented technology

The invention discloses a method for determining influence of exogenous dsRNA on toxicity of ladybugs. The method comprises the following specific steps: uniformly mixing the exogenous dsRNA into a sucrose solution, directly feeding the ladybugs for 2-3 days and then feeding with pea aphids; then detecting and analyzing the expression changes of target genes in the ladybugs, and observing the biological changes of the ladybugs so as to evaluate the toxicity of the exogenous dsRNA to the ladybugs, wherein the ladybugs include harmonia axyridis, coccinella septempunctata or henosepilachna pusillanima. By the method, direct influence of the exogenous dsRNA on the toxicity of the harmonia axyridis, the coccinella septempunctata or the henosepilachna pusillanima or other ladybugs can be effectively determined; in addition, the method is simple, feasible and high in effectiveness and sensitivity, and has a great significance and an application prospect in study on functions of related genesas well as environmental risk assessment on related RNAi transgenic crops.

The invention relates to an artificial fodder for imaginal harmonia axyridis. The artificial fodder is characterized by using pork liver homogenate, fly maggot powder, yeast extract, cane sugar, and honey as main components, and further containing 0.1-100mg/Kg of juvenoid hormone analogues. The juvenoid hormone analogues is added to the artificial fodder for harmonia axyridis, thereby stimulating the ovarian development and feeding of the harmonia axyridis, and the problem that the imaginal harmonia axyridis cannot spawn after being fed with ordinary artificial fodder is solved. Compared with the prior art, the fodder disclosed by the invention is simple in ingredient, easy to prepare, low in cost, and capable of guaranteeing the fertility of the imaginal harmonia axyridis, and the hatchability and the survival rate of the filial ladybird and the imago yield are high.

The invention discloses a diffusion breeding and releasing method for preventing myzus persicae through harmonia axyridis. The myzus persicae is bred in a diffusion breeding shed, and an insect-proof net (7) covers the diffusion breeding shed; a tobacco seedling is cultivated through a floating method; when the tobacco seedling has five to six true leaves, tobacco leaves with the myzus persicae are put on the tobacco seedling to breed the myzus persicae; male and female harmonia axyridis adults captured in the wild are put into the insect-proof net, eat the myzus persicae and then mate to lay eggs; the eggs are incubated into larvae, or low-age larvae are directly collected, or after the larvae are pupated, pupae are taken back for eclosion at room temperature; the eclosion adults are fed with the myzus persicae and are put into a refrigerator for refrigeration and storage; the low-age larvae or the adults of the harmonia axyridis are put into a low-age larva transporting and releasing device (9) or an adult transporting and releasing device (10); the low-age larvae or the adults of the harmonia axyridis are released to a field according to a ratio of the harmonia axyridis to the myzus persicae of 1:(100-120) in a preliminary forming stage of the myzus persicae in the field. According to the method, the breeding speed rate of the harmonia axyridis can be remarkably improved, and the field pest control efficiency is improved in a mode of preventing the myzus persicae through the harmonia axyridis.

The invention belongs to the field of feeding of natural enemy insects and in particular relates to an artificial feed for harmonia axyridis larvae and a preparation method of the artificial feed. The artificial feed for harmonia axyridis larvae is prepared from the following components in parts by weight: 5 parts of pork liver, 0.5-1.5 parts of dried powder of housefly larvae, 1-2 parts of yeast extract, 1-3 parts of saccharose and 1-2 parts of honey. The preparation method comprises the steps of preparing pasty pork liver, preparing dried powder of housefly larvae and mixing all the components. The components of the artificial feed are readily available, the investment is low, the production cost is low, and the preparation method is simple, can be operated easily and is suitable for feeding harmonia axyridis larvae in a large-scale manner.

The invention discloses a whole-course biological control method of tobacco diseases and insect pests. The method comprises the following steps: 1) in the seedling stage: using predatory mites for controlling thrips and tomato spotted wilf viruses; using harmonia axyridis and aphidius gifuensis for controlling myzus persicae and the thrips; using penicillium to inactivate mycelia, and 0.5% of chitosan and 0.5% to 2% of amino-oligosaccharin for controlling tobacco mosaic diseases; 2) in the field period: using the predatory mites and harmonia axyridis for controlling the thrips, the tomato spotted wilf viruses and the myzus persicae; using sex attractants for controlling prodenia litura adults and helicoverpa assulta adults; using Cahtheconidea furcellata for controlling prodenia litura larvae; using 0.5% of chitosan and 0.5% to 2% of amino-oligosaccharin for controlling the tobacco mosaic diseases; using Holder Burkholderia, and Pythium oligandrum for controlling tobacco black shank, black root rot, root knot nematode diseases and the like. The whole-course biological control method of the tobacco diseases and insect pests disclosed by the invention thoroughly eliminates pollution of chemical pesticides on the environment and tobacco pesticide residues and effectively controls the diseases and insect pests continuously and effectively, and the loss of tobacco quality for the diseases and insect pests is controlled within 5%.

The invention relates to the application of chouioia cunea larvae or pupae as a live feed to artificial breeding of harmonia axyridis. The breeding technology of the chouioia cunea larvae or pupae is mature, and one tussah pupa at least can breed 5,000 chouioia cunea larvae or pupae and at least can breed 100 harmonia axyridis. The breeding technology is easy to operate. The live feed for widely propagating the harmonia axyridis is simply and conveniently obtained. Artificial mass propagation of the harmonia axyridis can be realized.

The invention discloses an overwintering generation harmonia axyridis collection, low-temperature storage and release method. A negative suction type dust sucking device is selected as a device for collecting the overwintering generation harmonia axyridis; the collected harmonia axyridis is put in a refrigeration house or an ice tank for low-temperature storage, wherein the storage mode is that the collected harmonia axyridis is packaged into a cloth bag, and folded paper serving as a harmonia axyridis overwintering carrier is arranged inside the cloth bag; the release amount is determined based on the production of the control targets according to the seasonal production of the insects, and the harmonia axyridis is directly put on crops to be protected; and a box type device for transporting and releasing the harmonia axyridis is selected. A negative pressure device is selected as a collecting device to realize the rapid extensive collection of the overwintering generation harmonia axyridis; the refrigeration house or the ice tank is used for storage so as to realize long-term storage of the overwintering generation harmonia axyridis; the overwintering generation adults are selected as released insects; and the method solves the problem that two insect forms of the harmonia axyridis cannot meet the requirement on long-distance transport due to biological characteristics per se and restrict the release application of the harmonia axyridis.

The invention relates to a long-term storage method for overwintering generation harmonia axyridis, which comprises the following steps: trapped harmonia axyridis is loaded into a container with a hole, and the container is placed in a cellar for storage overwintering. The method is adopted to ensure that the storage period of the harmonia axyridis reaches more than eight months, and the overwintering survival rate of harmonia axyridis for egg production can reach 78 percent in the middle of May of the second year. The breakthrough of the key storage technology lays a foundation for the direct mass application of adult harmonia axyridis and the extension of the breeding period of the harmonia axyridis.

The invention relates to a method of utilizing a seedling growing greenhouse to propagate harmonia axyridis in a large number. The method includes following steps: a, sowing tobacco; b, inoculating myzus persicae; c, inoculating ladybird; d, collecting the ladybird; e, refrigerating; f, performing daily management. Tobacco seedling growing greenhouse facility is utilized to realize large-scale production of tobacco seedlings, and breeding cost is lowered; the tobacco seedling growing greenhouse facility is utilized to meet temperature, humidity and light conditions and isolating conditions needed by myzus persicae and the ladybird, and death rate of myzus persicae and the ladybird is lowered; through collection and exposure of pupa to the sun, convenence in collection and storage of harmonia axyridis is realized, and transportation and storage death rate of the ladybird is lowered; through refrigerating the pupa, large-number storage, all-time transportation and all-time release of harmonia axyridis and lowering of storage cost can be realized.

The invention relates to a method for mass propagation of harmonia axyridis by utilizing a formula of an alternative food and application and discloses a method for mass propagation of harmonia axyridis by utilizing anagasta kuehniella eggs and pork liver pulp as basal feed. At 18-32 DEG C, harmonia axyridis can grow normally, body weights are increased, spawning periods and service lives are prolonged, and the egg laying amount is improved and is 5004 at most and far exceeds the egg laying amount obtained by using conventional artificial feed or other natural prey. According to the method, foods are easy to store and high in utilization rate, the foods are easily used simultaneously with other artificial feed or natural prey, and the production cost is further reduced. The method is simple and convenient to sue and high in harvest rate.

The invention discloses a method for efficiently preventing and controlling diseases and pests of greenhouse watermelons. The method comprises the following steps of seed selection and seedling raising, seedbed management, preparation before greenhouse closing, greenhouse closing with lime nitrogen at high temperature, field planting, field management, disease and pest prevention and control and harvesting, wherein in the step of disease and pest prevention and control, the harm of soil-borne diseases to watermelons can be effectively prevented through soil sterilization, melons are prone to aphid harm, and natural enemy insects harmonia axyridis are utilized to control aphids; in the step of harvesting, timely harvesting is performed according to the natural maturity of watermelons. According to the method, organic fertilizers and the lime nitrogen are utilized to cooperate with high-temperature greenhouse closing, the natural enemy insects are utilized to replace pesticides and killaphids and other pests, soil sterilization is performed while organic fertilizers are fully composted, pollution-free pest control can be achieved, and the watermelon yield and quality can be also safely and efficiently improved.

The invention discloses a universal artificial feed for ladybirds and preparation and application methods of the universal artificial feed. The feed is prepared by mixing honey, citric acid anhydrous food grade and sunflower seed oil with Chinese oak silkworm pupae dry powder, flour weevil dry powder, peptone powder and aquae sterilisata as main raw materials. The preparation mode is simple and convenient, and the raw materials are cheap and available; the universal artificial feed is suitable for large-scale artificial feeding of the multiple ladybirds, such as coccinella septempunctata, harmonia axyridis, propylaea japonica; the multiple predacious ladybirds fed by the universal artificial feed are strong in vitality, are high in breeding capability, and are normal in filial generation growth index, and have the relatively high economical value.

The invention discloses a method for preventing vegetable aphid by means of Harmonia aryridis (Pallas). The method comprises a step of applying Harmonia aryridis (Pallas) on plants to be controlled with predator prey ratio of 1:(5 to 80). The experiment shows that applying Harmonia aryridis (Pallas) of different stages (egg, adult, 1-2 years larva and 3-4 years larva) on 2-leaf stage of broad bean plants with different predator prey ratio of (1:5, 1:10, 1:20, 1:40, and 1:80), the number of aphids on broad bean plants is obviously reduced at 1 to 11 days after inoculation, and the number can be reduced to zero, compared with plantes which are inoculated with Harmonia aryridis (Pallas). For aphid prevention on greenhouse capsicum (the predator prey ratio is 1:60), greenhouse sweet pepper (the predator prey ratio is 1:30), and open cauliflower (the predator prey ratio is 1:5 and 1:10) with Harmonia aryridis (Pallas) egg cards, good control effects are obtained. The method provided in the invention has advantages such as substantial effect and long duration; the method has wide application prospect in aphid biological control field.

The invention relates to a feed for raising Harmonia axyridis larvae. The feed comprises freeze-dried tussah powder, honey, sucrose, plant oil and freeze-dried aphid powder according to a weight ratio of (50-60):(20-30):(15-25):(0-2):(0-2). The above formula of the feed can meet nutrition demands of every age growth of Harmonia axyridis larvae. The feed has the advantages of low cost and easy obtaining of raw materials, convenient preparation, easy operation, factory production facilitation, and provision of technical and material bases for large-scale breeding and application of Harmonia axyridis.

The invention discloses a low-temperature storage method for artificial propagation of harmonia axyridis. The low-temperature storage method comprises the following steps: (1) exposing adult harmonia axyridis obtained through artificial propagation at 10 DEG C for twenty-four hours, and putting the adult artificial propagation in a ventilating device; (2) exposing and storing the harmonia axyridis obtained in the step (1) at -5 DEG C -5 DEG C, wherein interrupted high temperature exposure is conducted on the harmonia axyridis for 0.5-4 hours at 5 DEG C -30 DEG C everyday; (3) after low temperature storage and interrupted high temperature storage of the harmonia axyridis obtained in the step (2) are completed, transferring the harmonia axyridis into the feeding conditions and recovering the harmonia axyridis for more than twenty-four hours, and screening survival adult harmonia axyridis, wherein the artificial propagation conditions are as follows: temperature ranges from 24DEG C to 26 DEG C, humidity ranges from 60 percent to 70 percent, and the photoperiod L:D=16h:8h. By means of the low-temperature storage method, low temperature storage and interrupted high temperature storage are conducted on the harmonia axyridisd. Compared with constant low temperature storage, the survival rate can be remarkably increased.

The invention relates to a field large-scale propagation method for harmonia axyridis. The method is characterized by comprising the following steps: (a) preparing pea aphids; (b) feeding egg laying adults; (c) collecting egg masses; (d) hatching eggs; (e) managing first-instar larvae; (f) inoculating second-instar larvae to fields; (g) carrying out field management on harmonia axyridis; and (h) carrying out field release of harmonia axyridis. The method has the advantages that harmonia axyridis is raised in the fields, so that the artificial culture cost, field transportation cost and field release cost are reduced, and the adaptability of released harmonia axyridis to field climate is improved.

The invention discloses a stably expressed reference gene group of harmonia axyridis with different factors and application of the reference gene group. Specifically, a scheme of a stably expressed reference gene group of harmonia axyridis with different factors is that a stably expressed reference gene group under different temperature conditions comprises 18S, 28S and GAPDH (Reduced Glyceraldehyde-Phosphate Dehydrogenase), comprises 18S, 28S and HSP90 under different light cycle conditions, comprises 18S, 28S and HSP70 at different age stages, and comprises 18S, 28S and RP49 at different tissue combinations. By adopting the reference gene group, a most stable reference gene group of harmonia axyridis under four factors is confirmed for a first time, reference gene selection bases are made for study on gene expression levels of different tissues of different ages of harmonia axyridis under different temperature conditions and different light cycle conditions, and a solid base is madefor studying gene functions of the harmonia axyridis, RNAi (Ribonucleic Acid Interfere) transgenic crop environment risk estimation, and the like.

The invention relates to a breeding device and method for larvae of harmonia axyridis. The device specifically comprises a culture dish, a culture dish cover and a physical barrier device; the physical barrier device is placed in the culture dish and provided with an insect passing mechanism; the method comprises the steps of establishing a pea aphid population, breeding adult insects of harmoniaaxyridis with pea aphids, collecting eggs of harmonia axyridis, monitoring hatching of the eggs, newly adding aphids every day and breeding newly-hatched larvae until pupation. By means of the breeding device and method for the larvae of harmonia axyridis, the occupied space is small, intensive breeding of the larvae of harmonia axyridis is facilitated, and the breeding device and method are suitable for high-density larva breeding; the gathering degree and meeting possibility of the larvae are reduced in the breeding process, the autotomy rate is reduced accordingly, and the yield of productsof harmonia axyridis is increased; through intensive breeding, the production cost is reduced, the efficiency is improved, and the application and popularization of harmonia axyridis in biological prevention and control are promoted.

Insulin-like peptides (ILPs) in insects are widely involved in the regulation of various physiological processes such as growth, development and reproduction of the insects. Harmonia axyridis is an important predatory natural enemy insect. The invention discloses complementary deoxyribonucleic acid (cDNA) of an insulin-like peptide (ILP)-2 gene in the harmonia axyridis. After the eukaryotic expression of ILP-2 of the harmonia axyridis is realized, and the female adult of the harmonia axyridis is fed with the ILP-2, the egg production is increased by 20.2%. The ILP-2 of the harmonia axyridis can be used for the regulation of physiological processes such as spawning and diapause of the harmonia axyridis or other insects, and has a very broad application prospect in the large-scale productionof the insects.

The invention discloses artificial feed for harmonia axyridis larva as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) placing egg massof ampullaria gigas in a homogenizer, adding an appropriate amount of sterilized water for homogenization, filtering to remove egg shells to serve as a component A. (2) crushing multi-vitamin B and vitamin C, and filtering to remove large particles; (3) putting glucose, sucrose, the multi-vitamin B and vitamin C into a beaker, and adding distilled water for dissolving to serve as a component B; (4) uniformly mixing soy flour, rice stem borer pupa powder and yeast powder to serve as a component C; (5) heating agar for melting with water, then adding sorbic acid and honey, boiling, cooling, andadding cholesterol to serve as a component D for standby use; (6) mixing the component A, the component B and the component C together, stirring evenly, then adding the component D, and continuing tosufficiently stir uniformly. The preparation method disclosed by the invention has the characteristics of low price, economical and practical effects, simple preparation and the like, and is suitablefor large-scale, long-term and subculture feeding of standard test insects.