Asexual propagation method of gracilaria lemaneiformis

Abstract

The invention relates to an asexual propagation method of gracilaria lemaneiformis. The method comprises the following steps: (1) healthy and immature gracilaria lemaneiformis is selected, washed for multiple times, and then flushed with disinfected seawater to remove parasitic protozoan and epiphytic hybrid algas on the surface of the algae body, and after the gracilaria lemaneiformis is cultured for one week by using the disinfected seawater, the cultured gracilaria lemaneiformis is repeatedly washed until the microscopic examination result shows that the surface of the algae body is free of hybrid algas, the washed gracilaria lemaneiformis is soaked in a 1.5% KI solution for 10 minutes, then the soaked gracilaria lemaneiformis is washed with the disinfected seawater for 3 times, the washed gracilaria lemaneiformis is soaked for 10 seconds by using 70% alcohol, and then the soaked gracilaria lemaneiform is washed with the disinfected seawater for 3 times; (2) the treated gracilaria lemaneiformis algae body is cut into cutting sections of 2-4 mm by using a scalpel blade, and the cut gracilaria lemaneiformis is cultured in a PES culture solution containing 0.25mg / mL 6-BA in dark for 7 days; and (3) the culture condition is changed as follows: the temperature is 17 DEG C, the illumination intensity is 10 + / -2 [mu]E.m<-2>.s<-1>, the light cycle is 16h: 8h (Light: Dark). The asexual propagation method is simple and easy to operate and short in induction period, provides a technical method for manual breeding and seedling culture of the gracilaria lemaneiformis, and provides a support for seed production and propagation expanding.

Description

technical field [0001] The invention relates to a method for asexual reproduction of Gracilaria true. Background technique [0002] Gracilaria lemaneiformis belongs to the genus Gracilaria, which belongs to the class Florideae, the class Gigartinales, and the family Gracilariaceae. It is distributed all over the coast of my country. True Gracilaria is cylindrical and linearly branched. The branches are alternate and partial, and the base is slightly constricted. The base of each plant is a small disc-shaped fixator, and the main branch is thicker than the branch. The algal branches are thick and juicy. The color is reddish-brown, purple-brown, sometimes with green or yellow, and becomes dark brown after drying, and the algal branches shrink. Gracilaria true is an important economic seaweed, and its agar content is as high as 30%. Agriculture and medicine industry. As the demand for agar continues to increase, the raw material supply of true Gracilaria is far from suffi...

AI Technical Summary

Problems solved by technology

As the demand for agar continues to increase, the raw material supply of true Gracilaria is far from sufficient, and the problem of seed supply is still not completely resolved, thus limiting the development of artificial breeding

At present, there are few reports on tissue culture research of Gracilaria true