102results about How to "Short induction period" patented technology

The invention relates to a microorganism preparation used for eliminating the effect of anti-nutritional factors and is characterized by consisting of the following materials by weight percentage: 28 to 35 percent of bacillus subtilis, 45 to 53 percent of lactobacillus plantarum and 12 to 27 percent of yeast of brewer; a preparation method of the microorganism preparation comprises the following steps: the production of the strain is produced by combining the ray induction and production performance test, the production strain with high yield and large enzyme production are screened, the production technique adopts the technology of liquid single culture and solid composite culture, product accelerators are added into the culture medium so as to lead the product to be rich in active metabolites such as beneficial living bacterium, enzyme, peptide, polysaccharide and amino acids, and the like; after the feed material and complete feed are added with the microorganism preparation, the effect of anti-nutritional factors to the nutrient components in the feed can be eliminated greatly, the palatability of the feed is improved, feed intake is improved, stress resistance and immunity of the cultured animals is enhanced, group uniformity is improved, the growing speed and feed utilization can be improved and the culture cost can be reduced.

The invention discloses a new method of chloroethylene through acetylene method and specific equipment, which comprises the following steps: disposing raw material gas strictly; reacting two-segment compensation; recycling heptane; cooling; radiating; providing specific catalyst and reactor to synthesize chloroethylene; disposing effluent with mercury to obtain high-purity chloroethylene monomer.

The invention discloses a method of mediated transformation of an alfalfa by vacuum permeability auxiliary agrobacterium comprising (1) the seed sterilization and the test-tube plantlet culture, (2) the agrobacterium tumefaciens containing objective genes culture, (3) the vacuum permeability auxiliary agrobacterium infection, (4) the bacteriostasis and resistant plants selection and culture, (5) the resistant plants rootage selection, (6) the transformed plants hardening-seedling and the field transplantation or the like; the invention employs a transformation method by using the agrobacterium mediated transformation of the alfalfa young and tender true leaves and the vacuum permeability method, thereby greatly improving the transformation efficiency. The secondary resistance rootage of the resistance plants reduces the later detection workload and obtains a plurality of transformation plants. The method of the invention overcomes the difficulties of low genetic transformation efficiency and long transformation period of the conventional alfalfa, improves the alfalfa transformation efficiency by more than 95, shortens the transformation period 2-3 months and has important meaning for the theoretic research at the alfalfa genetic engineering aspect and the genetic breeding practical.

The invention discloses a pichia pastoris fermentation medium for large scale production of recombinant human collagen. 0.01-10g/L of a metabolism conditioning agent is used in the medium so that pichia pastoris metabolism is adjusted, recombinant protein expression is accelerated, a fermentation period is shortened, energy consumption is reduced, an error risk is reduced and stable industrial production is guaranteed. The medium realizes large scale production of recombinant human collagen through 1000L of a fermentation cylinder, shortens a methanol induction period by 20% and improves a recombinant human collagen expression level by 16.5%. The pichia pastoris fermentation medium is especially suitable for a pichia pastoris methanol induction stage of recombinant human collagen industrial high density fermentation expression, effectively reduces a production cost, produces substantial economic benefits and has a very important meaning for industrial production of the recombinant human collagen.

The invention relates to a low smell EPDM (ethylene propylene diene monomer) for an automobile wire harness and a preparation method thereof. The EPDM is prepared by the method of: (1) firstly addingEPDM into an open mill, then adding zinc oxide, stearic acid and an antioxidant RD in order, mixing the substances evenly, then further adding carbon black and paraffin oil, mixing the substances evenly, finally adding an environment-friendly accelerator and sulfur, and conducting mixing to obtain mixed compound; (2) placing the mixed compound in a die and conducting primary vulcanization to obtain a section of vulcanized EPDM; and (3) taking out the section of vulcanized EPDM for secondary vulcanization, thus obtaining the target product EPDM. Compared with the prior art, the low smell EPDM provided by the invention meets the characteristics of rubber itself in the aspects of strength, compression set and the like, at the same time also can meet the requirements of low emission and low smell, also takes into account the low pollution characteristic, and is especially suitable for making automobile parts and the like.

The invention relates to a regeneration method of a butene oxidative dehydrogenation catalyst, and mainly solves the problems of poor regeneration effect, decreased activity after regeneration, long induction period and shortened service life of the catalyst in the prior art. The method provided by the invention comprises the following steps: a) conducting carbon burning treatment on inactivated iron spinel composite oxide catalyst for butene oxidative dehydrogenation to produce butadiene at 360-480 DEG C under the conditions of oxygen / steam volume ratio of 0.01-0.06 and water vapor airspeed of 400-2000 / h for 2-24 h; b) treating the catalyst subjected to carbon burning at 300-550 DEG C under the conditions of gauge pressure of 0-0.2 MPa, oxygen / steam volume ratio of 0.01-0.1, water vapor volume space velocity of 400-4000 / h and sulfide gas phase concentration of 0-100 ppm for at least 24 h. The technical scheme well solves the problem, and can be used in industrial production of butadiene from butene oxidative dehydrogenation.

The invention relates to a synthesis melamine catalyst and manufacturing method. Used the following ways: the structure of the [Hz][Al2-xHx][Si4-yAly]012 commercial aluminum-silicon oxide and alumina market add to Kneading Machine, in Kneading process of accession water or acid solution, using pressure spray granulation, spouted bed granulator granulation or a crowded screening broken granulation process, It through drying, screening, activation processing to get the melamine catalyst; The commercial aluminum-silicon oxide of Structure [Hz][Al2-xHx][Si4-yAly]012 is X=0.15-0.2, Y=0.05, Z= X + Y. This invention catalyst has the advantages of high strength low attrition rate, low temperature catalyst, a catalyst has larger load for raw materials urea, catalyst with high selectivity, less by-product, short induction period. Preparation method is simple, low cost, with high cost-effectiveness.

The invention relates to a catalyst for preparing styrene through ethylbenzene dehydrogenation and a preparation method of the catalyst and mainly aims to solve the problems of lower initial activity and longer induction period of the catalyst in the prior art. The technical problems are better solved through the technical scheme that the catalyst and the preparation method thereof are adopted, wherein the catalyst is prepared by adding cerium sources including ammonium ceric nitrate and cerous oxalate as raw materials into a ferrum-potassium-cerium-molybdenum or tungsten catalyst system according to the required proportion. The catalyst can be used for the industrial production of styrene prepared through ethylbenzene dehydrogenation.

The invention discloses a method and kit for effectively inducing somatic cell phenotype reprogramming, and belongs to the field of a cell biology technique. According to the method and the kit, in accordance with problems in an existing somatic cell reprogramming method, a method, which is used for effectively inducing the somatic cell phenotype reprogramming into induced pluripotent stem cells by taking an extract of a natural active material, namely fish oocyte, as an inducing agent, is invented, and the method is applicable to the preparation of the animal pluripotent stem cells. The method and the kit of the invention can be used for effectively inducing the somatic cell phenotype reprogramming into the induced pluripotent stem cells; compared with other methods, the method is high in reprogramming efficiency on somatic cells, and the inducing method is simple, high in safety and relatively low in cost; and the method disclosed by the invention can solve the problem on the source of the pluripotent stem cells, and the method has broad clinical application value and prospect in such fields as regenerative medicine.

This invention provides a efficiently new induction model of hepatic oval cell. In this model significantly inducing the production of embryonic stem cell endogenous oval cell by using HGF and EGF, the whole induction method is fairly convenient, induction cycle is also very short. Utilizing very little embryonic stem cell provenance source can induce to generate sufficient hepatic oval cell which is contented to clinical requirement by using the invented induction model of hepatic oval cell, it also can be used for clinical category such as hepatic oval cell transplant, hepatic tissue engineering and so on, it can play a part in assistant supportive treatment or curing disease for Liver function failure, hepatic metabolic disease caused by various kinds of reason.

The invention discloses a method for preparing a multi-level-structured molecular sieve by taking a mesoporous material as an indirect template agent. The method comprises the following steps: mixing aluminum oxide spheres or silicon oxide microspheres, an external silicon source or an aluminum source, phosphoric acid, a template agent and water to form a crystallization raw solution; transferring the uniformly mixed crystallization raw solution into a hydrothermal kettle taking tetrafluoroethylene as a lining, and ageing; after ageing, putting the hydrothermal kettle into a homogeneous reactor for crystallization; cooling a crystallized solution, taking out the crystallized solution and carrying out washing and centrifuging treatment; filtering an obtained solid-liquid mixture, washing and drying; and roasting to remove the template agent to obtain the multi-level-structured molecular sieve. The molecular sieve comprises mesopores and micropores; generation and diffusion of isobutylene in pore channels can be inhibited very well; isomeric hydrocarbon and aromatic hydrocarbon, which have relatively great molecules, can be subjected to more serious diffusion limitation; and meanwhile, the diffusion resistance of a target product, low-carbon olefin, particularly propylene, is reduced due to the presence of the mesopores, so that the prepared multi-level-structured molecular sieve is used as a catalyst, is not easy to inactivate and has good stability.

The invention relates to an asexual propagation method of gracilaria lemaneiformis. The method comprises the following steps: (1) healthy and immature gracilaria lemaneiformis is selected, washed for multiple times, and then flushed with disinfected seawater to remove parasitic protozoans and epiphytic hybrid algas on the surface of the algae body, and after the gracilaria lemaneiformis is cultured for one week by using the disinfected seawater, the cultured gracilaria lemaneiformis is repeatedly washed until the microscopic examination result shows that the surface of the algae body is free of hybrid algas, the washed gracilaria lemaneiformis is soaked in a 1.5% KI solution for 10 minutes, then the soaked gracilaria lemaneiformis is washed with the disinfected seawater for 3 times, the washed gracilaria lemaneiformis is soaked for 10 seconds by using 70% alcohol, and then the soaked gracilaria lemaneiformis is washed with the disinfected seawater for 3 times; (2) the treated gracilaria lemaneiformis algae body is cut into cutting sections of 2-4 mm by using a scalpel blade, and the cut gracilaria lemaneiformis is cultured in a PES culture solution containing 0.25mg/mL 6-BA in dark for 7 days; (3) the culture condition is changed as follows: the temperature is 17 DEG C, the illumination intensity is 10 +/-2 [mu]E.m<-2>.s<-1>, the light cycle is 16h: 8h (Light: Dark). The asexual propagation method is simple and easy to operate and short in induction period, provides a technical method for manual breeding and seedling culture of the gracilaria lemaneiformis, and provides a support for seed production and propagation expanding.

The invention discloses a method for preparing a supported molecular sieve catalyst by using a vacuum impregnation method. A molecular sieve of a multi-stage pore structure is prepared by adopting a common MCM-41 or SBA-15 molecular sieve of a mesoporous structure as a core and SAPO-34 as a shell, and then the vacuum impregnation method is adopted to load metal nanoparticles; a molecular sieve carrier is firstly degassed before impregnation, and after air in the pore structure is removed, the carrier can be combined with a metal solution more tightly, so that dispersity of the metal nanoparticles on the carrier is improved, reduction of metal ion agglomeration in the reaction process is facilitated, the carbon deposition resistance of the molecular sieve catalyst is improved, the life of the molecular sieve catalyst is prolonged, and the problems of low target-product propylene selectivity and easy carbon-depositing inactivation of a supported molecular sieve catalyst which is preparedby using conventional microporous molecular sieves and the vacuum impregnation method are solved; the supported molecular sieve catalyst is also suitable for other conversion reactions.

The present invention relates to one kind of double-metal cyanide catalyst and aims at solving the problem of catalyst with longer induction period. The catalyst includes double-metal cyanide mixture, organic C4-C10 alcohol with tertiary alcohol structure and organic ester selected from fatty ester, aromatic monoester, aromatic biester and their mixture. It may be used in the industrial production of low unsaturation degree polyether polyol.

The invention relates to a magnesium modified chromium-based catalyst for vinyl polymerization and a preparation method of the magnesium modified chromium-based catalyst. The catalyst contains a chromium compound, a magnesium modified silica-gel carrier and an alkyl aluminum compound, wherein chromium accounts for 0.01wt%-5.0wt% of total mass of the catalyst, the magnesium modified silica-gel carrier accounts for 70.0wt%-99.9wt% of total mass of the catalyst, the mass ratio of silica gel to a magnesium compound in the carrier is 100-20:1, and aluminum accounts for 0.05wt%-25.0wt% of total massof the catalyst. When the catalyst is applied to industrial production of polyethylene, produced polyethylene particles are good in form, high in bulk density and wide in molecular weight distribution; the preparation method of the catalyst comprises steps as follows: a magnesium salt solution of tetrahydrofuran is taken as a dispersion medium, silica gel is taken as a carrier, the chromium compound is taken as an active component, an organo-aluminum compound is taken as a promoter, modification of carrier silica gel and loading of the active component are realized by spray drying, then drying, high-temperature activation, reduction, reactivation and re-reduction are performed, and the catalyst product is obtained; the technological process of the method is simple and convenient, no chromium-containing wastewater is produced, and industrial implementation is facilitated.

The invention provides a method for inducing embryogenic cells by taking african agapanthus leaves as explant, comprising the following steps: A, taking the leaf stalk bases of african agapanthus, washing and disinfecting, removing old leaves wrapping outside, and cutting off tissues at the upper and lower ends, so as to obtain leaf stalk base tissues; B, dicing the leaf stalk base tissues and then inoculating into a callus induction culture medium for culture; C, putting callus blocks obtained after culture in step B onto a callus subculture culture medium for subculture; and D, putting the callus blocks obtained after culture in step C onto an embryogenic callus induction culture medium for culture, so as to obtain the callus blocks containing mbryogenic cells. According to the invention, induction of the mbryogenic cells is successfully performed on the leaf stalk base tissues, the callus induction rate reaches 92.60%, the mbryogenic cell induction rate reaches 76.36%, and the induction cycle is only 6-7 months. The mbryogenic cell inducing seedling formation is rapid, a long-time subculture process is avoided, and occurrence of abnormal seedlings is reduced.

A catalyst for melamine production contains 30-55 wt% of Al2O3, 45-70 wt% of SiO2 and 0-10 wt% of P2O5. The Al2O3 comprises two parts, one of which is used as an active component and the other is usedas a binder. The SiO2 includes two parts, one part is used as a carrier of active component, and the other part is used as a binder. The P2O5 is used as a binder. The preparation method comprises thefollowing steps: adding water to the SiO2 carrier for pulping, adding the active component Al2O3, adding an acid solution to adjust the pH value, finally adding a binder, adopting spray granulation,roasting, washing and drying to obtain the melamine catalyst. The aluminum silicate melamine catalyst prepared by the invention not only has good physical and chemical properties, such as high strength (low abrasion), large water holes, large specific surface area adjustment range, but also has simple preparation method, less investment, no pollution emission, low production cost, low energy consumption, and remarkable economic and social benefits.