102results about How to "Short induction period" patented technology

The invention discloses a special foaming agent for a hard PVC (polyvinyl chloride) low-foaming material and a preparation method thereof. The special foaming agent comprises the following materials in parts by weight: 20-80 parts of azodicarbonamide (ADC), 20-80 parts of auxiliary foaming agent, 1-20 parts of activating agent, 0.2-10 parts of dispersing agent and 0.2-5 parts of processing agent. The preparation method comprises the steps: adding the dispersing agent and the processing agent in a mixer, mixing for 3-7 minutes, and sequentially uniformly mixing the ADC, the auxiliary foaming agent and the activating agent in the mixer to prepare the special foaming agent. The special foaming agent is suitable for hard PVC low-foam materials such as PVC crushing and low-foaming sectional bars, tubular products and sheets, a wood-plastics low-foaming material and the like. The special foaming agent has the positive effects that 1, the decomposition temperature is 160-190 DEG C, and is within a processing temperature range of the hard PVC low-foaming material; 2, the foaming process is short in induction period, the decomposition is performed without mutation, and the foaming process is easily controlled; 3, the hard PVC low-foaming material is used on a hard PVC low-foaming product, and has a smooth surface and has no discoloring and ageing phenomena; and 4, the special foaming agent is low in cost and good in comprehensive performance, and accords with the use requirement.

The present invention relates to a preparation method for microbial polypeptide preparation with the use of lactic acid bacteria and bacillus subtilis, and which is characterized in that the high yield enzyme and high quantity production strains is selected and trough the integration of original microwave-induction and rays-induction, wherein the production process applies the liquid-alone culture and solid-composite culture, adds special product promoter into the medium, the content of soybean peptide is high, at the same time which is rich in metabolic products such as peptidoglycan, microbial antioxidants and enzymes. The present invention has the advantage of low production cost, faster cycle, and faster absorption for animal after breeding, high daily increasing in weight, immunity enhancing, and stress rising, which has obvious effect in breeding and is a safe and high efficient new feed additive.

The invention relates to a chemical mechanical polishing abrasive particle CeO2 and a method for preparing the same, belonging to the rare earth powder material chemical preparation technical field. The invention is to prepare the CMP abrasive particle by utilization of cerous inorganic salt solution and homogeneous precipitation agent. The method comprises the following steps that: the cerous inorganic salt and the precipitation agent are prepared into solution with certain proportion; the solution is uniformly mixed through ultrasonic vibration; deposits are generated after the solution is heated to a certain temperature; serum is kept stand, aged, filtered and calcined, and then the CeO2 abrasive particle is prepared. The method also accelerates the nucleation rate through addition of surface active agent so as to reduce the reaction temperature, and simultaneously the nodulizing degree of the abrasive particle obtained is also good. The method prepares the CMP abrasive particle, wherein, the CMP abrasive particle belongs to the single-phase cubic crystal system; the space group is O<5>H-FM3M; the dispersibility is good; the grain fineness distribution is uniform; the shape is similar to a sphere; and the specific surface area BET is more than 0 and less than 50m<2> / g.

The invention relates to a microorganism preparation used for eliminating the effect of anti-nutritional factors and is characterized by consisting of the following materials by weight percentage: 28 to 35 percent of bacillus subtilis, 45 to 53 percent of lactobacillus plantarum and 12 to 27 percent of yeast of brewer; a preparation method of the microorganism preparation comprises the following steps: the production of the strain is produced by combining the ray induction and production performance test, the production strain with high yield and large enzyme production are screened, the production technique adopts the technology of liquid single culture and solid composite culture, product accelerators are added into the culture medium so as to lead the product to be rich in active metabolites such as beneficial living bacterium, enzyme, peptide, polysaccharide and amino acids, and the like; after the feed material and complete feed are added with the microorganism preparation, the effect of anti-nutritional factors to the nutrient components in the feed can be eliminated greatly, the palatability of the feed is improved, feed intake is improved, stress resistance and immunity of the cultured animals is enhanced, group uniformity is improved, the growing speed and feed utilization can be improved and the culture cost can be reduced.

The invention discloses a new method of chloroethylene through acetylene method and specific equipment, which comprises the following steps: disposing raw material gas strictly; reacting two-segment compensation; recycling heptane; cooling; radiating; providing specific catalyst and reactor to synthesize chloroethylene; disposing effluent with mercury to obtain high-purity chloroethylene monomer.

The invention provides a method for efficiently inducing reprogramming of human body cells into pluripotent stem cells, belonging to the field of biomedicine. The method provided by the invention comprises the following step: before carrying out nuclear reprogramming on body cells, culturing the body cells for 2-4 days in a culture medium containing thyroid hormone T3. The method provided by the invention can increase the iPSC (induced pluripotent stem cell) induction efficiency by 20-100 times to about 1.2%, and shorten the induction cycle to 12-16 days; and meanwhile, different clones of the iPSC obtained by induction have uniform cellular morphology, multiplication capacity and pluripotent gene expression, and can be used for researching human diseases.

The invention discloses a method of mediated transformation of an alfalfa by vacuum permeability auxiliary agrobacterium comprising (1) the seed sterilization and the test-tube plantlet culture, (2) the agrobacterium tumefaciens containing objective genes culture, (3) the vacuum permeability auxiliary agrobacterium infection, (4) the bacteriostasis and resistant plants selection and culture, (5) the resistant plants rootage selection, (6) the transformed plants hardening-seedling and the field transplantation or the like; the invention employs a transformation method by using the agrobacterium mediated transformation of the alfalfa young and tender true leaves and the vacuum permeability method, thereby greatly improving the transformation efficiency. The secondary resistance rootage of the resistance plants reduces the later detection workload and obtains a plurality of transformation plants. The method of the invention overcomes the difficulties of low genetic transformation efficiency and long transformation period of the conventional alfalfa, improves the alfalfa transformation efficiency by more than 95, shortens the transformation period 2-3 months and has important meaning for the theoretic research at the alfalfa genetic engineering aspect and the genetic breeding practical.

The invention discloses a method for inducing and differentiating a human embryonic stem cell into nerve cells. The method comprises the following steps of: separating and purifying a human embryonic stem cell to obtain human embryonic stem cell monomers; and inoculating the human embryonic stem cell monomers according to the cell density of (1-5)*104 / cm<2>, and performing single-layer adherence induced culturing, wherein in the induced culturing, nerve cells are obtained by pre-inducing the human embryonic stem cell with a sodium butyrate and an inducting culture medium I and performing differentiated induced culturing with an inducing culture medium. According to the method, the defects of high nerve cell heterogeneous degree, low differentiating efficiency, inconvenience for observing and operating in real time, need of a subsequent sorting process and the like existing in the conventional differentiating method can be overcome; the method has the advantages of easiness and convenience for operating, short inducting period (10-12) days, high nerve cell uniformity (the ratio is over 85 percent), and the like; and according to electrophysiologic monitoring, the nerve cells have convulsion discharging functions.

The invention discloses a pichia pastoris fermentation medium for large scale production of recombinant human collagen. 0.01-10g / L of a metabolism conditioning agent is used in the medium so that pichia pastoris metabolism is adjusted, recombinant protein expression is accelerated, a fermentation period is shortened, energy consumption is reduced, an error risk is reduced and stable industrial production is guaranteed. The medium realizes large scale production of recombinant human collagen through 1000L of a fermentation cylinder, shortens a methanol induction period by 20% and improves a recombinant human collagen expression level by 16.5%. The pichia pastoris fermentation medium is especially suitable for a pichia pastoris methanol induction stage of recombinant human collagen industrial high density fermentation expression, effectively reduces a production cost, produces substantial economic benefits and has a very important meaning for industrial production of the recombinant human collagen.

The invention relates to the preparation of a kind of low unsaturation polyether polyols. It's mainly used to solve the problem in previous technology that it has long catalyst inductive period and the concentration of catalyst in initiator is high, which is that it has a high catalyst cost in the preparation of unsaturation polyether polyols. The invention excellently solves the said problem through adopting catalyst components containing double metal cyanide (DMC) mixture, C4 - C10 organic mellow with tertiary alcohol structure, adding bronsted acid, and adopting the technology project of inert gas frothing to degasificate. So it can be used in the industrial production of low unsaturation polyether polyols.

The invention relates to a low smell EPDM (ethylene propylene diene monomer) for an automobile wire harness and a preparation method thereof. The EPDM is prepared by the method of: (1) firstly addingEPDM into an open mill, then adding zinc oxide, stearic acid and an antioxidant RD in order, mixing the substances evenly, then further adding carbon black and paraffin oil, mixing the substances evenly, finally adding an environment-friendly accelerator and sulfur, and conducting mixing to obtain mixed compound; (2) placing the mixed compound in a die and conducting primary vulcanization to obtain a section of vulcanized EPDM; and (3) taking out the section of vulcanized EPDM for secondary vulcanization, thus obtaining the target product EPDM. Compared with the prior art, the low smell EPDM provided by the invention meets the characteristics of rubber itself in the aspects of strength, compression set and the like, at the same time also can meet the requirements of low emission and low smell, also takes into account the low pollution characteristic, and is especially suitable for making automobile parts and the like.

The invention relates to a regeneration method of a butene oxidative dehydrogenation catalyst, and mainly solves the problems of poor regeneration effect, decreased activity after regeneration, long induction period and shortened service life of the catalyst in the prior art. The method provided by the invention comprises the following steps: a) conducting carbon burning treatment on inactivated iron spinel composite oxide catalyst for butene oxidative dehydrogenation to produce butadiene at 360-480 DEG C under the conditions of oxygen / steam volume ratio of 0.01-0.06 and water vapor airspeed of 400-2000 / h for 2-24 h; b) treating the catalyst subjected to carbon burning at 300-550 DEG C under the conditions of gauge pressure of 0-0.2 MPa, oxygen / steam volume ratio of 0.01-0.1, water vapor volume space velocity of 400-4000 / h and sulfide gas phase concentration of 0-100 ppm for at least 24 h. The technical scheme well solves the problem, and can be used in industrial production of butadiene from butene oxidative dehydrogenation.

The invention relates to a synthesis melamine catalyst and manufacturing method. Used the following ways: the structure of the [Hz][Al2-xHx][Si4-yAly]012 commercial aluminum-silicon oxide and alumina market add to Kneading Machine, in Kneading process of accession water or acid solution, using pressure spray granulation, spouted bed granulator granulation or a crowded screening broken granulation process, It through drying, screening, activation processing to get the melamine catalyst; The commercial aluminum-silicon oxide of Structure [Hz][Al2-xHx][Si4-yAly]012 is X=0.15-0.2, Y=0.05, Z= X + Y. This invention catalyst has the advantages of high strength low attrition rate, low temperature catalyst, a catalyst has larger load for raw materials urea, catalyst with high selectivity, less by-product, short induction period. Preparation method is simple, low cost, with high cost-effectiveness.

The invention relates to a catalyst for preparing styrene through ethylbenzene dehydrogenation and a preparation method of the catalyst and mainly aims to solve the problems of lower initial activity and longer induction period of the catalyst in the prior art. The technical problems are better solved through the technical scheme that the catalyst and the preparation method thereof are adopted, wherein the catalyst is prepared by adding cerium sources including ammonium ceric nitrate and cerous oxalate as raw materials into a ferrum-potassium-cerium-molybdenum or tungsten catalyst system according to the required proportion. The catalyst can be used for the industrial production of styrene prepared through ethylbenzene dehydrogenation.

The invention discloses a method and kit for effectively inducing somatic cell phenotype reprogramming, and belongs to the field of a cell biology technique. According to the method and the kit, in accordance with problems in an existing somatic cell reprogramming method, a method, which is used for effectively inducing the somatic cell phenotype reprogramming into induced pluripotent stem cells by taking an extract of a natural active material, namely fish oocyte, as an inducing agent, is invented, and the method is applicable to the preparation of the animal pluripotent stem cells. The method and the kit of the invention can be used for effectively inducing the somatic cell phenotype reprogramming into the induced pluripotent stem cells; compared with other methods, the method is high in reprogramming efficiency on somatic cells, and the inducing method is simple, high in safety and relatively low in cost; and the method disclosed by the invention can solve the problem on the source of the pluripotent stem cells, and the method has broad clinical application value and prospect in such fields as regenerative medicine.

The invention discloses a method for high-efficiency somatic embryogenesis and plant regeneration of camellia plants, comprising the following steps: excising 3 / 4 leaves of tissue culture seedlings ofcamellia plant tea tree, camellia oleifera or Camellia azalea and using stem segments with 1 / 4 leaf as explants, inoculating onto a somatic embryo induction medium, culturing at 24-26 DEG C under illumination to form somatic embryos at stem nodes and petioles; cutting the somatic embryos into slices of 1-5mm thick, tiling the slices onto a somatic embryo multiplication medium, and culturing at 24-26 DEG C under illumination to form new somatic embryos; transferring the somatic embryos onto a plant regeneration medium, and culturing at 24-26 DEG C under illumination to generate plantlets fromthe somatic embryos. By using the tender stem segments of camellia plants as the explants, a high-efficiency somatic embryogenesis and plant regeneration system is established, thereby effectively improving the tissue culture propagation efficiency of camellia plants. The method provides technical support for seedling production and genetic engineering improvement of crops such as tea trees, camellia oleifera, camellia, etc.

This invention provides a efficiently new induction model of hepatic oval cell. In this model significantly inducing the production of embryonic stem cell endogenous oval cell by using HGF and EGF, the whole induction method is fairly convenient, induction cycle is also very short. Utilizing very little embryonic stem cell provenance source can induce to generate sufficient hepatic oval cell which is contented to clinical requirement by using the invented induction model of hepatic oval cell, it also can be used for clinical category such as hepatic oval cell transplant, hepatic tissue engineering and so on, it can play a part in assistant supportive treatment or curing disease for Liver function failure, hepatic metabolic disease caused by various kinds of reason.

The invention discloses a method for preparing a multi-level-structured molecular sieve by taking a mesoporous material as an indirect template agent. The method comprises the following steps: mixing aluminum oxide spheres or silicon oxide microspheres, an external silicon source or an aluminum source, phosphoric acid, a template agent and water to form a crystallization raw solution; transferring the uniformly mixed crystallization raw solution into a hydrothermal kettle taking tetrafluoroethylene as a lining, and ageing; after ageing, putting the hydrothermal kettle into a homogeneous reactor for crystallization; cooling a crystallized solution, taking out the crystallized solution and carrying out washing and centrifuging treatment; filtering an obtained solid-liquid mixture, washing and drying; and roasting to remove the template agent to obtain the multi-level-structured molecular sieve. The molecular sieve comprises mesopores and micropores; generation and diffusion of isobutylene in pore channels can be inhibited very well; isomeric hydrocarbon and aromatic hydrocarbon, which have relatively great molecules, can be subjected to more serious diffusion limitation; and meanwhile, the diffusion resistance of a target product, low-carbon olefin, particularly propylene, is reduced due to the presence of the mesopores, so that the prepared multi-level-structured molecular sieve is used as a catalyst, is not easy to inactivate and has good stability.

The invention relates to an asexual propagation method of gracilaria lemaneiformis. The method comprises the following steps: (1) healthy and immature gracilaria lemaneiformis is selected, washed for multiple times, and then flushed with disinfected seawater to remove parasitic protozoans and epiphytic hybrid algas on the surface of the algae body, and after the gracilaria lemaneiformis is cultured for one week by using the disinfected seawater, the cultured gracilaria lemaneiformis is repeatedly washed until the microscopic examination result shows that the surface of the algae body is free of hybrid algas, the washed gracilaria lemaneiformis is soaked in a 1.5% KI solution for 10 minutes, then the soaked gracilaria lemaneiformis is washed with the disinfected seawater for 3 times, the washed gracilaria lemaneiformis is soaked for 10 seconds by using 70% alcohol, and then the soaked gracilaria lemaneiformis is washed with the disinfected seawater for 3 times; (2) the treated gracilaria lemaneiformis algae body is cut into cutting sections of 2-4 mm by using a scalpel blade, and the cut gracilaria lemaneiformis is cultured in a PES culture solution containing 0.25mg / mL 6-BA in dark for 7 days; (3) the culture condition is changed as follows: the temperature is 17 DEG C, the illumination intensity is 10 + / -2 [mu]E.m<-2>.s<-1>, the light cycle is 16h: 8h (Light: Dark). The asexual propagation method is simple and easy to operate and short in induction period, provides a technical method for manual breeding and seedling culture of the gracilaria lemaneiformis, and provides a support for seed production and propagation expanding.

The invention discloses a method for preparing a supported molecular sieve catalyst by using a vacuum impregnation method. A molecular sieve of a multi-stage pore structure is prepared by adopting a common MCM-41 or SBA-15 molecular sieve of a mesoporous structure as a core and SAPO-34 as a shell, and then the vacuum impregnation method is adopted to load metal nanoparticles; a molecular sieve carrier is firstly degassed before impregnation, and after air in the pore structure is removed, the carrier can be combined with a metal solution more tightly, so that dispersity of the metal nanoparticles on the carrier is improved, reduction of metal ion agglomeration in the reaction process is facilitated, the carbon deposition resistance of the molecular sieve catalyst is improved, the life of the molecular sieve catalyst is prolonged, and the problems of low target-product propylene selectivity and easy carbon-depositing inactivation of a supported molecular sieve catalyst which is preparedby using conventional microporous molecular sieves and the vacuum impregnation method are solved; the supported molecular sieve catalyst is also suitable for other conversion reactions.

The invention discloses a composite calcium-based catalyst and an application thereof. The composite calcium-based catalyst comprises the following components in percent by weight: 10%-40% of alkali metal hydroxide, 40%-80% of calcium salt and 10%-30% of cocatalyst. The composite calcium-based catalyst can be used for preparing polyethylene glycol with the molecular weight of more than 8000, is simple in process, strong in operability, easy in implementation, fast in reaction speed, and is beneficial to large-scale industrial production; simultaneously, the molecular weight of the prepared polyethylene glycol is accurate in control and narrow in molecular-weight distribution, no residual toxic metal ions are generated, so that the safety is good; in addition, the composite calcium-based catalyst has the advantages that the material is low in price, the activity is high and the induction period is short.

The present invention relates to one kind of double-metal cyanide catalyst and aims at solving the problem of catalyst with longer induction period. The catalyst includes double-metal cyanide mixture, organic C4-C10 alcohol with tertiary alcohol structure and organic ester selected from fatty ester, aromatic monoester, aromatic biester and their mixture. It may be used in the industrial production of low unsaturation degree polyether polyol.

The invention discloses a method for preparing an acraldehyde catalyst produced by selective dehydration of glycerol. The method comprises the following steps of: loading heteropolyacid cesium salt by using 5-60 percent of loading amount at the room temperature in a vacuum impregnation method by using niobium pentoxide as a carrier; and forging at 400-700 DEG C to prepare the catalyst, wherein the glycerol conversion rate of the catalyst is up to 75-100 percent, and the acraldehyde selectivity is up to 52-90 percent. The catalyst prepared by the invention is short in induction period, high in acraldehyde yield, long in service life and good in renewability, can recover the activity of the reaction by charking at 500 DEG C, and avoids inactivation after reaction for 200 hours.

The invention relates to a magnesium modified chromium-based catalyst for vinyl polymerization and a preparation method of the magnesium modified chromium-based catalyst. The catalyst contains a chromium compound, a magnesium modified silica-gel carrier and an alkyl aluminum compound, wherein chromium accounts for 0.01wt%-5.0wt% of total mass of the catalyst, the magnesium modified silica-gel carrier accounts for 70.0wt%-99.9wt% of total mass of the catalyst, the mass ratio of silica gel to a magnesium compound in the carrier is 100-20:1, and aluminum accounts for 0.05wt%-25.0wt% of total massof the catalyst. When the catalyst is applied to industrial production of polyethylene, produced polyethylene particles are good in form, high in bulk density and wide in molecular weight distribution; the preparation method of the catalyst comprises steps as follows: a magnesium salt solution of tetrahydrofuran is taken as a dispersion medium, silica gel is taken as a carrier, the chromium compound is taken as an active component, an organo-aluminum compound is taken as a promoter, modification of carrier silica gel and loading of the active component are realized by spray drying, then drying, high-temperature activation, reduction, reactivation and re-reduction are performed, and the catalyst product is obtained; the technological process of the method is simple and convenient, no chromium-containing wastewater is produced, and industrial implementation is facilitated.

The invention provides a method for inducing embryogenic cells by taking african agapanthus leaves as explant, comprising the following steps: A, taking the leaf stalk bases of african agapanthus, washing and disinfecting, removing old leaves wrapping outside, and cutting off tissues at the upper and lower ends, so as to obtain leaf stalk base tissues; B, dicing the leaf stalk base tissues and then inoculating into a callus induction culture medium for culture; C, putting callus blocks obtained after culture in step B onto a callus subculture culture medium for subculture; and D, putting the callus blocks obtained after culture in step C onto an embryogenic callus induction culture medium for culture, so as to obtain the callus blocks containing mbryogenic cells. According to the invention, induction of the mbryogenic cells is successfully performed on the leaf stalk base tissues, the callus induction rate reaches 92.60%, the mbryogenic cell induction rate reaches 76.36%, and the induction cycle is only 6-7 months. The mbryogenic cell inducing seedling formation is rapid, a long-time subculture process is avoided, and occurrence of abnormal seedlings is reduced.

The invention discloses a microbe additive and making method by using cholesterol metabolic path to modulate egg poultry through lactic acid bacteria, which is characterized by the following: utilizing bacillus subtilis to manufacture soybean polypeptide; reducing the content of yolk cholesterol; combining the microwave induction and ray induction to ferment bacteria; improving the sieving production and enzyme production; accelerating the growth of soybean polypeptide; improving the egg production and immunity of egg poultry; reducing the usage of antibiotics; providing a safe and high-effective agricultural biological agent.

The invention discloses a tissue culture and rapid propagation method of highly efficient induction of cluster buds of davidia involucrata. The method includes the specific steps: 1) selecting davidia involucrata branches as mother plants, wherein the davidia involucrata branches have robust growth, are free of pest and disease damage, have good characters and form lateral buds without leaf expansion, and soaking the davidia involucrata branches in a GA3 solution until the lateral buds of the davidia involucrata grow to 3 cm or above; 2) shearing the lateral buds, conducting disinfection, inoculating the lateral buds to a culture medium which contains Wpm, 1.2 mg / L<-1> of KT, 0.3 mg / L<-1> of NAA, 0.5 mg / L<-1> of 6-BA, 3.5 g / L<-1> of agar, 20 g / L<-1> of sucrose and 0.2 g / L<-1> of AC, and conducting cultivation for 18-22 days until bud points germinate and grow to form the cluster buds. By the use of a provided davidia involucrata cluster bud induction technology, the method is simple, the induction cycle is short, the propagation coefficient is high, and the method has a certain role in promoting the national level protection of rare and endangered plants.

The invention provides a preparation method of completely small molecule compound induced goat multipotential stem cells. The preparation method comprises the following steps: separation of goat ear-rim fibroblasts and rat fetus fibroblasts, preparation of a feed layer, preparation of a cell culture medium, production of goat induced multipotential stem cell-like clone, recognition of morphological characteristics of goat induced multipotential stem cell-like clone and identification of biological characteristics. By the preparation method of the completely small molecule compound induced goatmultipotential stem cells, goat somatic cell reprogramming is induced under the condition of completely small molecule compounds and zero serum, the induced multipotential stem cells without virus vectors or exogenous gene integration can be obtained, and good cell materials are provided for follow-up research. The preparation method is simple to operate, facilitates repetition, and is short in induction period; ingredients of a compound are clear; and the safety is quite high. The form of the goat induced multipotential stem cell-like clone obtained by the preparation method is similar to that of rat ESCs.

A catalyst for melamine production contains 30-55 wt% of Al2O3, 45-70 wt% of SiO2 and 0-10 wt% of P2O5. The Al2O3 comprises two parts, one of which is used as an active component and the other is usedas a binder. The SiO2 includes two parts, one part is used as a carrier of active component, and the other part is used as a binder. The P2O5 is used as a binder. The preparation method comprises thefollowing steps: adding water to the SiO2 carrier for pulping, adding the active component Al2O3, adding an acid solution to adjust the pH value, finally adding a binder, adopting spray granulation,roasting, washing and drying to obtain the melamine catalyst. The aluminum silicate melamine catalyst prepared by the invention not only has good physical and chemical properties, such as high strength (low abrasion), large water holes, large specific surface area adjustment range, but also has simple preparation method, less investment, no pollution emission, low production cost, low energy consumption, and remarkable economic and social benefits.