422results about How to "Guaranteed survival" patented technology

The invention provides a targeting sgRNA for pig CD163 gene editing, a modification vector, and a preparation method and application thereof, which relate to the technical field of gene engineering. The sgRNA and the gene modification vector provided by the invention have high specificity, and can be used for efficiently editing a pig CD163 gene on a cell level through a CRISPR/Cas9n system. According to the preparation method of a blue-eared disease resistant pig provided by the invention, a blue-eared disease virus receptor is damaged, the target gene CD163 is edited, no other exogenous gene is intruded, no non-specific editing is carried out on a non-CD163 gene area on a genome, a genetic background is clean and clear, and the work of later transgene safety evaluation is greatly reduced. The blue-eared disease resistant pig obtained by utilizing the invention can be ensured to survive normally, and resists the infection of a blue-eared disease virus, so that the economic loss caused by the blue-eared disease in pig husbandry is greatly reduced.

The invention discloses an integrated type ecological floating bed and the water body ecological system repairing technology thereof. A physical space of the integrated type ecological floating bed is formed by constructing a floating bed, a sinking bed, a buoyancy adjusting bottle, a lifting chain and an ecological barricading net, and integrates ecological components such as indigenous emergent aquatic plants, submerged plants, attached aquatic plants, fishes, snails and biochemical zeolite. The ecological behaviors of the ecological components are in linear cause and effect relation. The ecological components and organisms in the physical space form a semi-enclosed ecological subsystem with integral completeness. The upper line and the water body ecological system to be repaired are strong in coupling capability, and ecological factors in the lower line can exist stably. The damaged water body ecological system sufferring from one or a plurality of types of pollutions including water body eutrophication, heavy metal pollution, organic pollution and the like can be repaired by building pioneer communities and utilizing self-cleaning capability of the ecological system, pollution is removed, integral restoration or rebuilding of the damaged water body ecological system is promoted, simultaneously environment is beautified, and ecological deterioration of a healthy water body can be prevented.

The invention discloses a rescue capsule. The whole rescue capsule is arranged on a mobile chassis truck; a capsule body has a closed structure with a door, wherein a steel plate is calendared and welded with a reinforcing rib to form a section, and the structure is formed by connecting the sections through bolts and sealing rings; the interior of capsule is isolated into a main capsule and at least one isolated capsule; and an air curtain linkage system, an oxygen supply system, an environmental temperature control system, an air purification system, a power supply illumination support system, a communication device, a garbage and excrement device, a diet system and an emergency medical kit are arranged in the rescue capsule. Through the rescue capsule, persons in the capsule can live for long time in a disaster.

The invention discloses a method and a special device for live shrimp transportation. The method adopts cooling water to spray shrimps so as to finish transportation. In the special device, a flow separation device is mounted at the upper part of the inner side of an insulation casing, a plurality of trays, a biological filter element, a support plate with uniformly distributed through holes and a water tank are sequentially arranged at the lower side of the flow separation device, and a water pump is arranged in the water tank and can feed water in the water tank to the flow separation device by a water pipe; an oxygen-transmitting hole is arranged at the inner side wall of the insulation casing, and an oxygen-transmitting electric valve tube is communicated with an oxygen-transmitting hole pipeline; a semiconductor refrigeration device penetrates through the insulation casing, and the water pump, the semiconductor refrigeration device, and a temperature probe and an oxygen sensor in the insulation casing are electrically connected with an automatic control device for controlling temperature and oxygen. The invention has the advantages of reasonable structure, space conservation and small consumption of water for transportation, avoids the drug anesthesia of the shrimps, can prevent the shrimps from pricking each other to death due to scare in a transportation process, and the like.

The invention provides a method for cultivating earthworms. The method comprises the steps that first, a base box body is sequentially filled with river stones, gravel and silver sand, an earthworm cultivation box is placed on the base box body, organic waste mixtures are arranged in the earthworm cultivation box, then earthworm seedlings are put into the earthworm cultivation box, after the earthworms are cultivated for 30 days, organic waste mixtures are continuously added into the earthworm cultivation box until the earthworm cultivation box is filled with the organic waste mixtures; earthworm cultivation boxes are continuously placed on the earthworm cultivation box filled with the organic waste mixtures, after the earthworm cultivation box at the top layer is filled with the organic waste mixtures, the earthworm cultivation box at the bottom layer is drawn out, and the earthworm cultivation box is placed back to the top layer after earthworm cast in the earthworm cultivation box is cleaned away to achieve circulation cultivation. The method for cultivating the earthworms has the advantages that the facts that earthworms are bred from generation to generation, are inbred and are in variety degeneration are avoided, and breed conservation and reproduction of the earthworms can be better achieved. Adult earthworms are harvested conveniently, the living and incubation environment of the earthworms is not damaged, the incubation rate is guaranteed, and the method is suitable for earthworm industrial large-scale cultivation.

The invention discloses a solar energy powered intelligent flowerpot and a using method. The solar energy powered intelligent flowerpot comprises a pot body, a lifting support and a moving plate, wherein the pot body comprises an inner pot and an outer pot; a temperature sensor and a humidity sensor are arranged on the wall of the inner pot; the inner pot is divided into an upper layer and a lower layer by a sealed partition; a water tank is arranged between the upper layer of the inner pot and the outer pot; a water pump, a singlechip and a storage battery are mounted on the lower layer of the inner pot; a solar cell panel and an illumination sensor are distributed on the upper surface of the moving plate; an LED light source and a water spraying hole are arranged on the lower surface of the moving plate; the water pump is connected with the water spraying hole through a water tube; the temperature sensor, the humidity sensor and the illumination sensor are connected with the singlechip; and the water pump, the lifting support and the LED light source are controlled by the singlechip. Temperature, humidity and illumination can be detected automatically, lighting supplementing and watering are carried out automatically, and therefore, a potted plant can survive normally under the condition that the potted plant is not attended by people in a short time; and the solar energy powered intelligent flowerpot not only can be used as the flowerpot, but also can be used as a flowerpot type desk lamp.

The invention relates to a method for treating osteoarthritis by stem cells and a composition. Particularly, the invention relates to a cell treatment composition for treating osteoarthritis and cartilage defects. The composition includes mesenchyma stem cells, hyaluronic acid or pharmaceutical salt, sodium chloride and water. The invention also relates to a method for preparing the composition. The cell treatment composition has the excellent properties.

The present invention provides a production process of fermented and concentrated feed for animal, and the feed is produced with soybean dregs, peanut dregs, corn protein powder, calcium biphosphate, salt, trace elements and leaven in certain weight proportion. The production process includes the steps of: activating and cloning spawn to prepare spawn liquid; mixing the spawn liquid and the said materials to ferment for 15-30 days. The corresponding production apparatus is also provided. The fermented and concentrated feed has increased lactic acid bacteria, saccharomycetes, enzymes, coarse protein, amino acid, etc. and can result in excellent animal feeding effect.

The invention provides an automatic vending machine which comprises a vending machine box body (92), an automatic vending machine rotating material channel (2), an electronic control device and an automatic vending machine refrigerator (1). The automatic vending machine rotating material channel (2) and the automatic vending machine refrigerator (1) are both arranged in the vending machine box body (92), and the automatic vending machine rotating material channel (2) can rotate and is provided with a plurality of display platforms. The electronic control device is used for controlling the automatic vending machine rotating material channel (2) to rotate, and the automatic vending machine refrigerator (1) comprises a humidifier (11). The automatic venting machine further comprises an automatic vending machine rotating material channel detecting mechanism (6), one or more automatic closing type material taking opening doors (7), an insulation plate and the like. According to the automatic vending machine, a specific automatic vending machine structure is arranged, flowers and similar goods can be vended, especially, the temperature and humidity of the flowers can be ensured, and therefore the flowers can survive.

The invention relates to a programmable young animal frightening stimulus stress experiment system. The system comprises a stress experiment equipment subsystem and an electric appliance control subsystem, and is characterized in that the stress experiment equipment subsystem and the electric appliance control subsystem are installed in the same sealed sound-insulation box; the electric appliance control subsystem is an electromechanical integrated structure composed of an electronic stimulus control part and a programmable frightening stimulus signal time controller; and the electric appliance control subsystem is installed at top part of an experiment equipment isolation box. The system has the beneficial effects that: the defects in the prior art are overcome; by structure innovation, electric striking without blind zones is achieved as well as wind stimulus and odor stimulus are increased; the programmable experiment system can conveniently provide multiple quantized young animal stress reflection stimulus signals, and research the mouse stress reflex behavior under the condition that multiple mice undergo various frightening stimuli in the same environment; and the stimulus integration scheme can be freely defined according to the preset corresponding stimulus stress environment.

The present invention discloses probiotics enriched crisp apple products and a preparation method thereof, and belongs to the technical field of agricultural product processing. Apples are used as raw materials, the apples are washed, the washed apples are blanched, a vacuum soaking method is used to enrich probiotics into apple tissues, and then a vacuum freeze-drying and vacuum microwave combined drying method is used to prepare the probiotics enriched crisp apple products. The method comprises the following steps: (1) raw material washing and blanching; (2) probiotic soaking liquid preparing; (3) vacuum soaking; and (4) vacuum freeze-drying and vacuum microwave combined drying. The prepared probiotics enriched fruit and vegetable crisp products are mellow in fruit fragrance, and crisp and tasty. The viable probiotic number can be maintained at 10<7> CFU/g at 25 DEG C for 90 days or more. The preparation method avoids the two major defects of high cholesterol risk of the probiotic fermented milk products and the non-consumption of lactose intolerant population, and the products combine health care and leisure, and have broad market prospects.

A high-density aquatic product transporting and breeding device comprises a fish tank, a water pump, a water purification system, a constant temperature system and an oxygenating system, wherein a water inlet pipe and a water outlet pipe are arranged in the bottom of the fish tank; the water inlet pipe and the water outlet pipe are connected through a pipeline to form a water circulating loop; and the water pump, the water purification system, the constant temperature system and the oxygenating system are arranged on the pipeline of the water circulating loop, wherein the water purification system comprises a hydraulic cyclone and a filter system or one of the two; the constant temperature system is a dividing wall type heat exchanger, and a heat exchange medium is a low-temperature fluid body or a high-temperature fluid body from a refrigerating unit; a liquid oxygen bottle is arranged on the oxygenating system, and liquid oxygen in the liquid oxygen bottle generates gaseous oxygen through a pressure reducing valve and inputs the gaseous oxygen to the oxygenating system; overflow holes and an overflow trough are formed on the fish tank, so that the content of body fluid proteins and fine impurities in live fish transporting and breeding process can be effectively reduced. The device provided by the invention can be used for transporting and breeding high-density live fishes and increases the quantity of fishes in the fish tank as more as possible on a basis of guarantee of survival fishes, so that the transporting and breeding cost is lowered.

The invention discloses a wide-range optical fiber vacuum sensor with multiple steps and a manufacturing method of the wide-range optical fiber vacuum sensor with the multiple steps. The wide-range optical fiber vacuum sensor comprises a sensor head, a sensor body, a transmission optical fiber, a supporting structure of the sensor and an accommodating structure of the transmission optical fiber. The sensor head is a stepped optical fiber vacuum sensor head and provided with a dual-layer structure with SiO2 oxide layer steps. The first layer structure is a monocrystalline silicon wafer, the second layer structure is a Pyrex glass wafer, a two-level stepped round shallow pit array is arranged on the front surface of the Pyres glass wafer, the Pyres glass wafer is divided into single sensor units, wherein the surfaces of the sensor units are squares or other polygons, and the round shallow pit array comprises a first round shallow pit step and a second round shallow pit step. The wide-range optical fiber vacuum sensor achieves high precision pressure measurement in a vacuum region, can survive in a constant pressure range without other packaging measures, can be used for high precision measurement in the constant pressure range, can effectively expand the high precision pressure measurement range of the sensor, and is convenient to manufacture in a massive mode.

The invention discloses a traditional Chinese medicine wastewater treatment process. The process has the following beneficial effects: by adopting the method of connecting activated sludge aerobic pools and contact oxidation aerobic pools in parallel and in series in the step of biochemical reflux, treatment of wastewater at different CODCr concentrations can be effectively handled and effective treatment of the wastewater can be ensured at high CODCr concentration; the stability of a wastewater treatment system can be ensured when the wastewater is at low CODCr concentration; the adaptive capacity of the wastewater treatment system is comprehensively improved; and good economic and environmental benefits are obtained.

The invention relates to a construction method of a fin cell line of anabarilius grahami and belongs to the technical field of freshwater aquatic organism cell culture. The method comprises that the anabarilius grahami tail fin tissue cell serves as a material, a tissue block method is adopted, and the primary culture is strated, culturing is achieved in a dulbecco modified eagle medium (DMEM)/F12 containing fetal calf serum and human basic fibroblast growth factors and having a PH value ranging between 7.0 and 7.4; and subculture is performed by using a trypsin digestion method. The method has the advantages that 1, the minimally invasive processing is adopted, on the premise that the fish is alive, and the operation is easy and feasible; 2, the anabarilius grahami fin muscle cell line (AGF) is in a fibroblast cell patternin a fiber structure, continuous passage can be achieved, the line can be applied to biological characteristic research directly, and requirement for anabarilius grahami molecular cell level theoretical research is met; and 3, the construction method is also applicable to cell lines of constructed fin rays of other fishes.

The invention provides to a microorganism-containing fertilizer produced by a mycoderm method and a production method of the fertilizer. In the prior art, microorganism bactericides, organic matters, chemical fertilizer and the like are mixed according to a preset formula, and the mixture is subjected to granulation, high-temperature drying, screen separation, cooling and the like so as to produce a finished product. In the production method provided by the invention, the fertilizer without bactericides is granulated, dried, screened and cooled; active bacterium powder is coated on the outer surface of the fertilizer particles at the tail end of the production line by using a film technology; and the microbial content of the whole fertilizer when in detection reaches the technical standard of national regulation so that a film fertilizer is formed. The method provided by the invention is used for producing the fertilizer with active bacterium.

The invention discloses a new rain water treating technique-a road biological treatment method. In the technique, a biological filter material containing microorganisms is put under a penetrable road and a moist layer; a matrix mixed by stone, mineral slag, ceramisite and the like, according to certain grade is mixed with the microorganisms so as to produce special biological filter material roadbed for rain water treatment; and when rain water flows through the penetrable road, the pollutant in the rain water can be treated under the biochemical effect of the microorganisms, thereby realizing the effect of purifying water. Without influencing road function, the purification treatment, collection and reuse of road rain water in urban roads, squares, parking lots and the like are realized. The method pertains to the field of environment-friendly water treatment.

The invention discloses a high-nutrient microbial bacterium composite fertilizer and a production method thereof. The high-nutrient microbial bacterium composite fertilizer comprises the following components in parts by weight: 16-190 parts of double-wall layer composite microbial bacterium microcapsules (containing 3-5 parts of composite microbial bacteria), 150-450 parts of monoammonium phosphate, 0-400 parts of urea powder, 100-200 parts of potassium chloride, 200-350 parts of ammonium chloride, 20-100 parts of ammonium bicarbonate, 0-150 parts of ammonium sulfate and 0-50 parts of attapulgite. The production method comprises the following steps: adsorbing the microbial bacteria by adopting germ powder thereof, performing first wall layer embedding by adopting a chemical cross-linking method, performing second wall layer embedding by adopting a spray drying process, and finally coating on the surface of chemical fertilizer granules, and split charging. The composite fertilizer is sufficient in nutrient and high in viable count, so that the microbial bacteria can be slowly released, yield increase of crops is guaranteed, the incidence rate of insect pests is reduced, and soil hardening caused by application of chemical fertilizers is improved.

The invention discloses a fiber grating sensor insert for injection molding product detection and a manufacturing method thereof. The insert comprises a temperature fiber grating, a strain fiber grating, grating encapsulators, protection layers and connectors; the temperature fiber grating and the strain fiber grating are arranged side by side, the temperature fiber grating comprises a plurality of conducting fibers and a plurality of grating unit zones, the strain fiber grating comprises a plurality of conducting fibers and a plurality of grating unit zones, the conducting fibers and the grating unit zones are disposed at intervals, the grating encapsulators protecting the grating unit zones are disposed on the temperature fiber grating and the strain fiber grating, the protection layers wrap the conducting fibers, and ends of the temperature fiber grating and the strain fiber grating are provided with the connectors. The insert can be conveniently and reliably implanted in a product injection molding process, so a temperature sensor and a strain sensor are buried in a product, the sensors can be positioned, and the sensors can be guaranteed to be live in a high-temperature and high-voltage condition of injection molding; and the sensors are seamlessly embedded into the product, so measurement accuracy and the service lives of the sensor can be substantially improved.

The invention discloses a method for measuring virulence by feeding Bemisia tabaci Gennadius imago, belonging to the technical field of insect feeding. The method comprises: (1) preparing and sterilizing a synthetic medium; (2) processing leaves by a medicament, and disinfecting and planting petioles; (3) adsorbing and releasing the Bemisia tabaci Gennadius imago; and (4) feeding, observing and the like. The invention can truly simulate the growing situation of leaves on plants, and can prolong the freshness of the leaves, and the medium has small possibility of going mouldy, thus effectively preventing the test imago of the Bemisia tabaci Gennadius from dying in a non-virulence mode; the contrast Bemisia tabaci Gennadius can normally survive within 72 hours of normal virulence measurement to obtain the standardization of the experiment test imago. The method is suitable for the requirements on measuring virulence by feeding Bemisia tabaci Gennadius imago for flowers, vegetables and cash crops.

The invention relates to the technical field of household appliances, in particular to a preservation refrigerator having a function of keeping aquatic organisms alive. The preservation refrigerator comprises a living thing preservation chamber arranged in a refrigerator body and further comprises an ion generator and a ventilation facility, wherein a drawer for accommodating water is arranged in the living thing preservation chamber, the ion generator is installed in the living thing preservation chamber, used for purifying air in the living thing preservation chamber and improving oxygen content in the living thing preservation chamber and has sterilization and peculiar smell removal functions, and the ion generator and the ventilation facility are connected with a refrigerator control system. The independent living thing preservation chamber is arranged in the refrigerator and is matched with the ion generator and a filter device to keep the aquatic organisms alive under the low-temperature condition, and the refrigeration quality of aquatic food materials are improved. In addition, the refrigerator is provided with a temperature sensor, the water in the drawer is kept to be at set temperature, a circulation fan is arranged to facilitate the uniformity of overall temperature in the living thing preservation chamber and increase the amount of dissolved oxygen in the water.