The invention provides a strawberry stem tip detoxification
tissue culture method for lowering the aberration rate. The method includes the following steps that a general induction culture medium for different
gene types of strawberries is prepared,
primary culture is conducted on strawberry stem tips, and a
virus-free
plant is detected and screened to serve as a multiplication stock
plant; a general multiplication culture medium of different
gene types of strawberries is prepared; the multiplication stock
plant is subjected to multiplication culture; then rooting culture is conducted, and acclimatizating and
transplanting are conducted. According to the strawberry stem tip detoxification
tissue culture method, a general culture medium formula which is suitable for conducting stem and tip
tissue culture on different types of strawberries is determined, the induction culture medium and the first multiplication culture medium are unified into one type, it is unnecessary to screen different culture medium formulas for different varieties, the multiplication culture medium formula lowers the use concentration of
cytokinin (6-BA), and thus generation of a great number of weak and small
bud plexuses and thin and long and invalid tissue culture seedlings is avoided; besides, an alternate multiplication culture method is adopted, the number of times of secondary culture is controlled to maintain at eight, generation of variation is reduced, and standard production is easy to conduct.