33 results about "Corneal nerve" patented technology

Methods and apparatus for the treatment of the eye to reduce pain can treat at least an outer region of the tissue so as to denervate nerves extending into the inner region and reduce the pain. For example, the cornea of the eye may comprise an inner region having an epithelial defect, and an outer portion of the cornea can be treated to reduce pain of the epithelial defect. The outer portion of the cornea can be treated to denervate nerves extending from the outer portion to the inner portion. The outer portion can be treated in many ways to denervate the nerve, for example with one or more of heat, cold or a denervating noxious substance such as capsaicin. The denervation of the nerve can be reversible, such that corneal innervation can return following treatment.

The invention provides methods and compositions for minimizing, preventing, or treating damage to corneal nerves by administering to a subject with such damage or at risk of exposure to such damage a composition which blocks an activity of an IL-1 cytokine and / or an IL-17 cytokine.

The invention provides methods and compositions for minimizing, preventing, or treating damage to corneal nerves by administering to a subject with such damage or at risk of exposure to such damage a composition which blocks an activity of an IL-1 cytokine and / or an IL-17 cytokine.

Methods and apparatus for the treatment of the eye to reduce pain can treat at least an outer region of the tissue so as to denervate nerves extending into the inner region and reduce the pain. For example, the cornea of the eye may comprise an inner region having an epithelial defect, and an outer portion of the cornea can be treated to reduce pain of the epithelial defect. The outer portion of the cornea can be treated to denervate nerves extending from the outer portion to the inner portion. The outer portion can be treated in many ways to denervate the nerve, for example with one or more of heat, cold or a denervating noxious substance such as capsaicin. The denervation of the nerve can be reversible, such that corneal innervation can return following treatment.

The present invention discloses a fungal keratitis image recognition method based on RX anomaly detection and texture analysis. The method comprises a step of obtaining a normal corneal nerve image and a mycelium image which comprises mycelium only as a training sample, a step of obtaining the fundus image of a fungal keratitis patient as a test sample, a step of carrying out preprocessing, feature extraction and feature integration on the normal corneal nerve image in the training sample to obtain a nerve feature after training sample integration, a step of carrying out preprocessing, feature extraction and feature integration on the mycelium image which comprises the mycelium only in the training sample to obtain a mycelium feature after the training sample integration, a step of carrying out preprocessing, feature extraction and feature integration on the image in the test sample to obtain the nerve feature after test sample integration and the mycelium feature after the test sample integration, and a step of recognizing the nerve and mycelium in the test sample.

The invention discloses a corneal nerve curvature measuring system and method based on an IVCM image. The measurement system comprises an image acquisition and preprocessing module, a corneal nerve segmentation module, a nerve segmentation curvature calculation module, a polymerization parameter selection module, a nerve segmentation curvature polymerization module, an analysis result display module and the like. The system is different from an existing end-to-end model based on deep learning. A process that a doctor estimates the curvature grading of the whole image according to some representative nerve segments in the living body confocal microscope image is simulated; the curvature of each nerve segment in the living body confocal microscope is aggregated by adopting multiple parameter-adjustable functions, the solvability is high, the correlation with a doctor evaluation result is high, the doctor can directly adjust the aggregation parameters in combination with professional knowledge, the adjustment process is visual and transparent, the application in auxiliary clinical diagnosis is facilitated, and the practicability is high.

The present invention discloses the application of nerve polypeptide PACAP38 in the preparation of eye disease curing, injury repair or reproduction drugs. Compared with symptomatic treatment artificial tear aiming at corneal epithelium repair or tear streaming delay and the like at the existing market, by researching the function of the nerve polypeptide PACAP38 in corneal nerve repair and reproduction after rabbit eximer laser in-situ keratomileusis and the function of the nerve polypeptide PACAP38 for rabbit trigeminal ganglion cell proliferation and differentiation, the nerve polypeptide PACAP38 with certain concentration has an obvious growth promotion function for corneal nerve after an LASIK operation and rabbit trigeminal ganglion cells cultured outside a body; and thus, the present invention provides a new plot for xerophthalmia curing, cutting operations of cataract and eximer laser photorefractive keratectomy, and corneal nerve injures caused by other eye and systematic diseases.

The present invention is directed to a software algorithm that measures the number of corneal nerve fibers in images captured by microscopy including images from patients obtained by in vivo corneal confocal microscopy, a noninvasive technique. The present invention solves a complicated segmentation problem, by exploiting the piece wise linear nature of the nerve fibers—i.e., the nerves are made up of a lot of straight line segments. The image is split into sub-regions, where each sub-region contains nerves mostly running in the same, straight direction. Having the nerves all in straight-lines within a single 2d image region dramatically simplifies the segmentation problem. The image intensities are summed in the direction of the nerves to reduce the 2d representation to a 1d signal having pronounced peaks where the nerves are located.

The present invention relates to a biological deriving amniotic membrane, composite biological deriving amniotic membrane and method for making same, as a material used for repair engineering of skins, corneas and nervous tissues and clinical application, especially for the biological deriving amniotic membrane treated through a de-cell process and the composite biological deriving amniotic membrane formed by recombination with collagen sponges. The biological deriving amniotic membrane and the composite biological deriving amniotic membrane prepared by adopting the process in accordance with the present invention keep the complete spatial structure of amniotic membrane and entirely remove cells therein, with a very low antigen character. The biological deriving amniotic membrane and the composite biological deriving amniotic membrane in accordance with the present invention are used for defending tissue adhesions in surgical operations, biological dressings, eye surface transplantation and disease treating in tissue engineering skins, corneas, nerves and the like; can guard against tissue adhesions, promote cell adhesions, shorten the time for skin healing, alleviate patient aches, rebuild normal structure of eye surfaces, without leading to immunoreactions and with high security.

The embodiment of the invention provides an eyeball cornea nerve segmentation method and device based on a convolutional neural network model. The method comprises the following steps: acquiring an eyeball cornea layer image of an in-vivo confocal microscope; inputting the eyeball cornea layer image of the in-vivo confocal microscope into a preset convolutional neural network model to obtain an eyeball cornea nerve segmentation result corresponding to the eyeball cornea layer image of the in-vivo confocal microscope and a parameter result corresponding to the eyeball cornea nerve segmentation result, wherein the parameter result comprises a length parameter, a width parameter and a density parameter of corneal nerves of eyeballs. According to the embodiment of the invention, the cutting and parameter calculation of the corneal nerve fiber can be completed at the same time, so that the automatic cutting and parameter evaluation of the corneal nerve can be realized quickly and efficiently, and the auxiliary diagnosis process of ocular surface diseases such as dry eyes can be realized.

The invention belongs to the field of neural image processing, and discloses a neural image curvature estimation method based on a topological structure, and the method comprises the steps of obtaining corneal neural image information, and separating the corneal neural image information through a deep learning network to obtain corneal neural network segments; calculating a curvature index of thecorneal neural network segment, and performing curvature index aggregation on the corneal neural network segment by adopting a weighted average aggregation algorithm to obtain a curvature aggregationindex; establishing a corneal nerve topological structure based on corneal nerve image information; analyzing the anisotropy of the corneal neural network segment, and fusing the curvature polymerization indexes by adopting an induced generalized ordered weighted average method to obtain the curvature of the corneal neural image; according to the invention, the morphological structures and functional anisotropy of different corneal nerve segments are fully considered, individualized analysis is performed on different nerve branches, clinical diagnosis experience and corneal nerve curvature automatic calculation can be effectively combined, and therefore clinical diagnosis based on corneal nerve images is achieved.

It is intended to provide a drug of a novel type for restoring the corneal sense after keratotomy or improving the dry eye symptom. Application of a somatostatin receptor agonist is useful in improving corneal dysesthesia following cataract operation, following LASIK operation, or associating corneal nerve degeneration such as neuroparalytic keratitis, corneal ulcer or diabetic keratopathy, and the dry eye symptom.

The invention provides methods and compositions for minimizing, preventing, or treating damage to corneal nerves by administering to a subject with such damage or at risk of exposure to such damage a composition which blocks an activity of an IL-1 cytokine and / or an IL-17 cytokine.

The invention discloses astragaloside eye drops with high water solubility and high stability. The astragaloside eye drops comprise astragaloside serving as a main medicine, and are characterized by further comprising rebaudioside A and glycyrrhizic acid which serve as medicine auxiliary materials; the mass ratio of the astragaloside to the rebaudioside A ranges from 1:15 to 1:30; and the mass ratio of the glycyrrhizic acid to the rebaudioside A ranges from 1:3 to 1:6. According to the astragaloside eye drops, the administration concentration can reach 10 mg / ml, micelles have small grain diameter and are distributed uniformly, the medicine stability is high, the administration irritation property is obviously reduced, and the medicine absorbed by cornea is increased; moreover, the rebaudioside A and the glycyrrhizic acid have anti-diabetic and anti-inflammatory activities, so that the astragaloside eye drops have a good medicine effect of coordinating eye treatment diabetic cornea lesion and corneal nerve lesion, and the astragaloside eye drops are highly economical.

The invention discloses a U-shaped network and a segmentation method of nerve fibers in a cornea image, the U-shaped network comprises four layers of encoders and decoders, and the encoders and the decoders are symmetrically connected in a cross-layer manner; a multi-scale separation and fusion module is added after the upsampling operation of the decoder part; according to the U-shaped network, the parameter quantity is reduced, the receptive field is increased, and the segmentation performance is improved; the segmentation method adopts the trained U-shaped network, and the loss function adopts the combination of the fiber length difference between the prediction graph and the gold standard and the Dice loss to jointly constrain the U-shaped network. According to the method, fine cornealnerve fibers can be accurately segmented, and the segmentation precision of the corneal nerve fibers is improved.

The invention discloses a system for intelligently analyzing corneal nerve fibers by using confocal microscope images in vivo, including: an information recording module; a corneal image acquisition module for collecting several confocal microscope images of each layer of the cornea; an information extraction module; Sieve module, locate confocal images of abnormal corneal tissue from each corneal confocal image through the first neural network model; feature analysis module; feature comparison module; disease grading module, including disease course severity grading module and disease outcome grading module The course severity grading module is that all corneal confocal pictures pass through the second neural network model to obtain the course severity level; Return to grade; result sending module. The invention analyzes the characteristics of corneal nerve fibers, the degree of damage and the changing process thereof, and evaluates the state of local or systemic neuropathy.

The topical administration of a combination of nerve growth factor (NGF) and docosahexaenoic acid (DHA) has been discovered to synergistically increase the effects of NGF in re-innervating the cornea. This enhancement in corneal nerve re-growth will yield a faster anatomical and functional recovery after PRK or LASIK surgeries. Using rabbits, the application of NGF and DHA resulted in increased corneal nerve surface area, increased epithelial proliferation, and decreased rose bengal staining as compared with NGF, DHA, or vehicle control individually. The topical application of NGF plus DHA in accelerating the re-innervation after PRK or LASIK, will help avoid or alleviate the symptoms of dry eye or other neurotrophic keratopathies due to corneal injuries. The topical application can be by using a corneal shield or lens. This treatment will also be useful in other corneal abnormalities including those caused by chemical burn, congenital corneal neuropathy, or acquired corneal neuropathy.

The invention discloses an image recognition method for fungal keratitis based on the AMBP improved algorithm, which comprises the following steps: using the RX abnormal detection algorithm to preprocess and binarize the mycelium image and the normal corneal nerve image, and then perform expansion and corrosion processing , strengthen the hyphae and neural characteristic information in the image; improve the AMBP algorithm, calculate the mean value of the pixels in the analysis window except the center pixel, and use it as a new parameter; calculate the average value and median value of the pixels in the analysis window respectively pixels, calculate the variance between the two and the rest of the pixels in the analysis window, and use the difference as a new discriminant condition to extract image texture features; use the extracted features to train a classifier, and use this classifier to identify images acquired by confocal microscopes. Identify nerves and hyphae in images.

The invention relates to a light-sheet microscopy for corneal nerve imaging and belongs to the technical field of corneal nerve imaging. The light-sheet microscopy comprises a control device, an illumination light path and an image acquisition light path. The illumination light path injects light into the cornea of the eye, forming a dynamic light sheet at an angle of alpha with the horizontal plane at the position of the cornea of the eye; an image signal is transmitted into the image acquisition light path at an angle of beta with the horizontal plane, and the image acquisition light path sends the image signal into the control device for imaging, wherein the angle between the angle of alpha and the angle of beta is 90 degrees, and the angle of alpha is unequal to 90 degrees. The dynamiclight-sheet microscopy is used to acquire a corneal nerve image, accelerate an imaging speed, reduce the shooting time, and avoid the injury to eyes caused by illumination light directly shining thefundus.

Provided herein are methods of reducing corneal endothelial cell loss (e.g., nerve loss-related corneal endothelial cell loss) that include selecting a subject identified as having an eye with reduced numbers of corneal nerves as compared to a reference eye, e.g., an eye of a healthy control, and administering vasoactive intestinal peptide (VIP) or a nucleic acid encoding VIP to the selected subject.

The invention relates to an annular microneedle for ophthalmology, and belongs to the technical field of biology. The invention provides a microneedle. The microneedle comprises a needle body and a substrate, the needle body is fixed on the base; the base is annular, and the shape and the size of the base are matched with those of the sclera edge; the microneedle acts on the sclera margins of human canthus, eye diseases are treated by rapidly penetrating into the corneal stroma layer and dissolving and releasing contained drugs, the bioavailability of ocular surface drugs is remarkably improved, meanwhile, compared with a traditional non-invasive multi-array square microneedle, the microneedle is small in size, does not affect the light transmission of the center of the cornea, and can be applied to the field of ophthalmology. The stimulation to corneal nerves in a central area is smaller, the problems that a traditional non-invasive multi-array square microneedle can cause reduction of corneal translucency and is prone to causing corneal pain can be effectively solved, and the biological safety of eye microneedle administration and patient compliance are improved.

The invention provides application of Sirt1 in repairing corneal nerve injury. It is discovered herein that Sirt1 may promote the repairing of corneal nerve injury and may also promote the preparing of corneal epithelial injury as well as the axonal growth of trigeminal ganglion cells; the Sirt1 may be used to culture trigeminal ganglion cells, repair persistent corneal epithelial defect, repair diabetic corneal nerve injury and repair nervous keratitis. Moreover, corneal nerve injury can be more effectively repaired by the joint use of silencing signal regulator 1 and miR-182-5p agonist.

The invention discloses an application of rhFGF-21 in preparing a medicine for treating dry eye syndrome. rhFGF‑21 improved basal tear secretion, BUT, corneal fluorescein sodium staining and corneal nerve sensitivity in a dose-dependent manner in mice with dry eye induced by scopolamine combined with dry environment, which opened up a new method for the treatment of dry eye.

The invention discloses a recombinant human collagen, a coding gene and an application of the recombinant human collagen in preparation of a biodegradable collagen-based corneal substitute, and the recombinant human collagen and the coding gene are obtained by treating the recombinant human collagen and a high-molecular polymer in a water phase in a composite cross-linking manner and curing and molding in a mold, or after the recombinant human collagen and the high-molecular polymer are subjected to electrostatic spinning to form a nanofiber membrane, the nanofiber membrane is further subjected to composite crosslinking and drying to obtain the three-dimensional corneal scaffold, and platelet-rich plasma is added for use after rehydration swelling in clinical application. The cornea substitute is free of virus hidden danger, free of immunogenicity, controllable in degradability and good in refraction and mechanical strength, and has a remarkable induction effect on regeneration of corneal epithelial cells, corneal bodies and corneal nerves after being added with platelet-rich plasma (APRP).

A multichannel stimulation system for regenerating damaged corneal nerves, includes: a multichannel unit including a first channel formed of a conductive material and attached between an area above a left eye and a left eyebrow to transfer a stimulation signal, and a second channel formed of a conductive material and attached between an area above a right eye and a right eyebrow to transfer the stimulation signal; and a stimulation signal module for providing the first channel and the second channel with an electric pulse signal as the stimulation signal, wherein the multichannel unit includes a contactor formed between the first channel and the second channel in one piece to electrically contact with the stimulation signal module.

The invention discloses a fungal keratitis image recognition method based on RX abnormality detection and texture analysis, comprising: acquiring normal corneal nerve images and mycelium images containing only hyphae as training samples; acquiring fundus images of patients with fungal keratitis As a test sample; preprocessing, feature extraction and feature fusion are performed on the normal corneal nerve image in the training sample to obtain the neural features after training sample fusion; Extraction and feature fusion to obtain the mycelium features after training sample fusion; preprocessing, feature extraction and feature fusion to the image in the test sample to obtain the neural features after the test sample fusion and the mycelial features after the test sample fusion; Identify nerves and hyphae in test samples.

The present invention is directed to a software algorithm that measures the number of corneal nerve fibers in images captured by microscopy including images from patients obtained by in vivo corneal confocal microscopy, a noninvasive technique. The present invention solves a complicated segmentation problem, by exploiting the piece wise linear nature of the nerve fibers—i.e., the nerves are made up of a lot of straight line segments. The image is split into sub-regions, where each sub-region contains nerves mostly running in the same, straight direction. Having the nerves all in straight-lines within a single 2d image region dramatically simplifies the segmentation problem. The image intensities are summed in the direction of the nerves to reduce the 2d representation to a 1d signal having pronounced peaks where the nerves are located.

The invention relates to the field of biological medicine, in particular to application of a compound LM22B-10 in preparation of a medicine for repairing corneal epithelium and nerve trauma. The invention discloses application of the compound LM22B-10 in preparation of a medicine for treating corneal epithelium and nerve injury, and belongs to the field of biological medicine. Pharmacodynamic tests prove that after normal mice with corneal epithelium injury and type I diabetic mice are taken as experimental subjects and LM22B-10 eye drop administration is carried out, the length and density of corneal nerve fibers are remarkably increased, the corneal epithelium recovery speed is obviously increased, and the corneal sensitivity is basically recovered to be normal. The compound has a relatively good treatment effect on corneal nerve injury caused by keratitis, xerophthalmia, full femtosecond laser myopia correction, excimer laser surgery, diabetes and the like, and eye symptoms of patients can be relieved; and the drug toxicity is small, the stability is good, and important development and application prospects are realized.