Application of nerve polypeptide PACAP38 in the preparation of eye disease curing, injury repair or reproduction drugs

A neuropeptide, nerve injury technology, applied in nervous system diseases, sensory diseases, drug combinations, etc., can solve the problems of eye injury repair or regeneration that have not been seen in PACAP38 eye diseases, and achieve simple development and design solutions and avoid side effects. , the effect of promoting nerve recovery

Inactive Publication Date: 2010-06-23
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, there are no relevant reports on the application of PACA

Method used

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  • Application of nerve polypeptide PACAP38 in the preparation of eye disease curing, injury repair or reproduction drugs
  • Application of nerve polypeptide PACAP38 in the preparation of eye disease curing, injury repair or reproduction drugs
  • Application of nerve polypeptide PACAP38 in the preparation of eye disease curing, injury repair or reproduction drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1 LASIK surgery rabbit model preparation

[0040] Rabbits were anesthetized with 3% pentobarbital ear vein injection (15-30mg / kg), fixed in a proper position, cut off the long eyelashes around the eyelids, routinely laid towels, 75% alcohol and 0.5% iodophor to disinfect the eyelids and orbits week skin.

[0041] Preoperative local anesthesia: right eye, 0.4% topical anesthesia pupil drops tobucaine hydrochloride, conjunctival sac, 1 time after fixation, 1 time after eyelid opening. No surgery was done on the left eye.

[0042]Formulation of corneal flap: open the eyelids with the eyelid opener, absorb the eyeball with the negative pressure fixing ring, and moisten the cornea with normal saline. The keratome makes a corneal flap with a thickness of about 130 μm and a diameter of about 6 mm (the pedicle is located at the top). BSS was used to flush the corneal flap before and after the operation (to eliminate scattered debris). Under the microscope, the co...

Embodiment 2

[0043] Embodiment 2PACAP38 eye drops and corneal sensitivity measurement

[0044] PACAP38 is a commercially available product with a molecular weight of 4356.8, and each bottle contains 0.1mg of PACAP38 (0.23×10 -4 mmol), PBS was injected into the bottle equipped with PACAP38 with a syringe, and diluted into eye drops of two specifications:

[0045] (1) 5 μM / L eye drops: the concentration of PACAP38 in the eye drops is 5 μM / L, and the dosage of PBS is 0.0046L;

[0046] (2) 10 μM / L eye drops: the concentration of PACAP38 in the eye drops is 10 μM / L, and the amount of PBS used is 0.0023 L.

[0047] Filter the eye drops to remove bacteria, pack them in separate packages and put them in a 4°C refrigerator for later use.

[0048] This embodiment is divided into three experimental groups:

[0049] a. PBS group: 5 rabbits, 1 drop of PBS was applied to the right eye of the rabbits in each group, 4 times / day, starting from the first day after LASIK operation to 8 weeks after operati...

Embodiment 3

[0055] Example 3 Modified Bielschowsky method of silver nitrate staining and immunohistochemical staining and observation of corneal nerve

[0056] Modified Bielschowsky silver nitrate staining:

[0057] This embodiment is divided into three experimental groups:

[0058] a. PBS group: 5 rabbits, 1 drop of PBS was applied to the right eye of the rabbits in each group, 4 times / day, starting from the first day after LASIK operation to 4 weeks after operation. The left eye of each rabbit does not drip;

[0059] b. 5 μ M / L eye drop group: 5 rabbits, 1 eye drop of 5 μ M / L specification (preparation method is the same as embodiment 2) is used in the right eye of each group of rabbits, 4 times / day, since the first day after LASIK From 1 day to 4 weeks after operation. The left eye of each rabbit does not drip;

[0060] c.10 μ M / L eye drop group: 5 rabbits, 1 eye drop of 10 μ M / L specification (preparation method is the same as embodiment 2) is used in the right eye of each group o...

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Abstract

The present invention discloses the application of nerve polypeptide PACAP38 in the preparation of eye disease curing, injury repair or reproduction drugs. Compared with symptomatic treatment artificial tear aiming at corneal epithelium repair or tear streaming delay and the like at the existing market, by researching the function of the nerve polypeptide PACAP38 in corneal nerve repair and reproduction after rabbit eximer laser in-situ keratomileusis and the function of the nerve polypeptide PACAP38 for rabbit trigeminal ganglion cell proliferation and differentiation, the nerve polypeptide PACAP38 with certain concentration has an obvious growth promotion function for corneal nerve after an LASIK operation and rabbit trigeminal ganglion cells cultured outside a body; and thus, the present invention provides a new plot for xerophthalmia curing, cutting operations of cataract and eximer laser photorefractive keratectomy, and corneal nerve injures caused by other eye and systematic diseases.

Description

technical field [0001] The present invention relates to the field of treatment of eye diseases, in particular to the application of polypeptide drugs in the treatment of eye diseases, and in particular to the application of neuropeptide PACAP38 in the preparation of eye disease treatment, damage repair or regenerative medicine. Background technique [0002] Excimer laser corneal refractive surgery has entered ophthalmology clinics since 1988, and has taken off glasses for countless patients since it was used to correct refractive errors. LASIK is still the mainstream of corneal refractive surgery, and postoperative dry eye is the main factor affecting postoperative visual quality and patient satisfaction. [0003] The main cause of dry eye after LASIK is that the corneal nerve is damaged to varying degrees during the operation, which affects the perception of the cornea, which in turn leads to abnormal tear secretion dynamics and decreased tear film stability, and the repair...

Claims

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Application Information

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IPC IPC(8): A61K38/17A61P27/02A61P25/00
Inventor 丁勇赵秀丽余榕捷陈建苏
Owner JINAN UNIVERSITY
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