Method for preparing tissue engineering cornea

A tissue engineering and cornea technology, applied in the field of tissue engineering cornea preparation, can solve the problems of the amniotic membrane that has been removed from the cell components cannot exert amniotic membrane stem cells, the source of corneal limbal stem cells is limited, and the clinical application effect is not good, and the ability to proliferate and differentiate Strong, easy to change shape size and thickness, low cost effect

Active Publication Date: 2010-06-30
SHAANXI RUISHENG BIOTECH
View PDF3 Cites 24 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The current tissue-engineered cornea mainly uses amniotic membrane with decellularized components as a carrier, and limbal stem cells are inoculated on the surface to construct it. The main disadvantage is that the source of limbal stem cells is limited, and t

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0014] The technical solution of the present invention will be further described in detail below in conjunction with specific examples.

[0015] Step 1. Prepare the corneal scaffold: Obtain the porcine corneal tissue, peel off the tissue around the cornea, wash it with PBS solution and freeze it at -80°C for 1 hour, and then thaw it at room temperature. Repeat the freezing and thawing process three times to completely rupture the cells. Disintegration; soak in pure water at 4°C for 1 day, swell it and cut to 1 / 2 thickness; then place it in 0.2% (w / v) protease solution for 2 hours, and rinse with pure water 3 to 5 times ; Place it in 0.5M NaOH solution and soak for 20 minutes to dissolve cells and inactivate viruses. Rinse with PBS solution until pH is neutral; Place it in containing 40U / ml DNA enzyme and 30U / ml α-galactide Soak in the mixed solution of glycosidase for 30 minutes to remove residual DNA and α-galactosyl antigen components, reduce immunogenicity, rinse with PBS solu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for preparing tissue engineering cornea. Amniotic epithelial stem cells and mesenchymal stem cells are adopted as seed cells; after in-vitro induced differentiation, the seed cells are planted on both surfaces of a natural acellular corneal stroma; and the tissue engineering cornea is formed by in-vitro organ culture. A scaffold used in the method overcomes the difficulty of large immunological rejection of a xenogenic corneal stroma and reserves the structure (capable of restoring the transparence of the cornea) and main components (comprising growth factorscapable of promoting the cornea to grow, proliferate and differentiate) of the natural cornea. Compared with a product in the prior art, the cornea has the advantages of low cost, convenient operation, wide source and easy storage; and the tissue engineering cornea containing living cells has certain elasticity and tenacity, easy change of shape size and thickness and extremely low immunogenicity, avoids complications caused by non-corneal materials, can be reformed by receptor cells after being implanted into a body, can be quickly integrated with an organism for realizing transparency, and is suitable for restoring cornea damage caused by various reasons.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering of biological materials, and in particular relates to a preparation method of tissue engineering cornea. Background technique [0002] The anatomical characteristics of the eyeball determine that the position of the eyeball is exposed, the tissue structure is fragile, and a small external force or foreign body can cause serious damage. In addition, some corneal diseases include infectious keratopathy, corneal degeneration, malnutrition and immune keratopathy, etc. Can cause corneal damage. Once damaged, it will seriously affect vision or cause blindness. Corneal lesions are the second leading cause of blindness next to cataracts, and the number of cases is increasing at a rate of 1.5 million to 2 million cases per year. While keratoplasty is the most effective means of treating corneal lesions, donors for traditional keratoplasty mainly come from cadavers and donations. At present, t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61L27/38A61L27/36
CPCC12N2506/02C12N2533/90A61L2430/16A61F2/142C12N5/0605C12N5/0621
Inventor 王爱军
Owner SHAANXI RUISHENG BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap