Method for preparing tissue engineering cornea

Abstract

The invention relates to a method for preparing tissue engineering cornea. Amniotic epithelial stem cells and mesenchymal stem cells are adopted as seed cells; after in-vitro induced differentiation, the seed cells are planted on both surfaces of a natural acellular corneal stroma; and the tissue engineering cornea is formed by in-vitro organ culture. A scaffold used in the method overcomes the difficulty of large immunological rejection of a xenogenic corneal stroma and reserves the structure (capable of restoring the transparence of the cornea) and main components (comprising growth factorscapable of promoting the cornea to grow, proliferate and differentiate) of the natural cornea. Compared with a product in the prior art, the cornea has the advantages of low cost, convenient operation, wide source and easy storage; and the tissue engineering cornea containing living cells has certain elasticity and tenacity, easy change of shape size and thickness and extremely low immunogenicity, avoids complications caused by non-corneal materials, can be reformed by receptor cells after being implanted into a body, can be quickly integrated with an organism for realizing transparency, and is suitable for restoring cornea damage caused by various reasons.

Description

technical field

[0001] The invention belongs to the technical field of tissue engineering of biological materials, and in particular relates to a preparation method of tissue engineering cornea. Background technique

[0002] The anatomical characteristics of the eyeball determine that the position of the eyeball is exposed, the tissue structure is fragile, and a small external force or foreign body can cause serious damage. In addition, some corneal diseases include infectious keratopathy, corneal degeneration, malnutrition and immune keratopathy, etc. Can cause corneal damage. Once damaged, it will seriously affect vision or cause blindness. Corneal lesions are the second leading cause of blindness next to cataracts, and the number of cases is increasing at a rate of 1.5 million to 2 million cases per year. While keratoplasty is the most effective means of treating corneal lesions, donors for traditional keratoplasty mainly come from cadavers and donations. At present, t...