21results about How to "Reduce immune rejection" patented technology

The invention provides double-crosslinking acellular matrix hydrogel as well as a preparation method and application thereof, and belongs to the technical field of hydrogel. The preparation method comprises the following steps: (1) carrying out decellularization treatment on a porcine dermal layer to obtain a decellularized porcine dermal matrix, (2) putting freeze-dried powder of the decellularized porcine dermal matrix into an acidic pepsin solution, stirring and digesting, adjusting the concentration, adding 10xPBS, adjusting the pH value to obtain a pregel solution, and carrying out freeze-drying on the pregel solution to obtain the porcine dermal matrix freeze-dried powder. (3) putting gel formed by the pre-gel solution into a tannic acid solution for cross-linking to obtain mono-cross-linked acellular matrix hydrogel; (4) smashing the mono-cross-linked acellular matrix hydrogel, and mixing the smashed mono-cross-linked acellular matrix hydrogel with a glucose oxidase solution and a ferrous ion solution with the concentration of 0.1-10mg / ml to obtain a bi-cross-linked acellular matrix hydrogel precursor; 5, the double-crosslinking acellular matrix hydrogel precursor is placed under the condition of glucose with the concentration being 10-200 mg / dl to form the double-crosslinking acellular matrix hydrogel. The hydrogel prepared through the method can achieve the balance of safety, supporting performance and degradability, and is wide in application range.

The invention is applicable to the technical field of hemodialyzers, and provides a hollow fiber membrane hemodialyzer with a gradient aperture structure, which comprises a shell, a hollow fiber membrane bundle packaged in the shell, an end cover and a sealing component, the hollow fiber membrane bundle is composed of a plurality of gradient aperture hollow fiber membranes; the hollow fiber membrane is of a two-way gradient aperture structure along the membrane thickness direction; a compact screening layer and a loose transition layer are sequentially arranged on the inner wall of the hollow fiber membrane; a loose diffusion layer and a compact protective layer are sequentially arranged on the outer side of the hollow fiber membrane; the pore diameter of the compact protective layer is not greater than 30nm; the porosity of the hollow fiber membrane is synchronously and progressively increased along with the aperture gradient, the porosity range is 40%-80%, the dialyzer has the characteristics of bidirectional gradient aperture optimization design, gradient functional coating protection, modular adaptation, special-shaped section synergy and the like, and the technical problems that an existing dialyzer is incomplete in toxin removal, insufficient in safety and the like are solved.

The invention discloses an application of engineered citrobacter freundii in fermentation synthesis of a stable body. The engineered citrobacter freundii is citrobacter freundii overexpressing a polyphosphate kinase gene Ppk1. The stable body prepared by fermenting the engineered citrobacter freundii is rich in polyphosphates and polyamines, particularly, the content of the polyamines can be up to 21wt%, the polyphosphates in the stable body can store the polyamines in an ionic bond combination mode, the combined polyamines are insoluble in water, a natural anion coating is generated, and the stability of the stable body is improved. And the sustained-release tablet is not easily taken by intestinal epithelial cells, so that the sustained-release tablet has a certain sustained-release effect. The medicine prepared by taking the compound as a main active ingredient shows a remarkable curative effect in ulcerative colitis treatment, has the comprehensive effects of reducing oxidative stress, enhancing the anti-inflammatory ability of a body and regulating immunity, is suitable for patients of all ages, and has outstanding application value and market prospect.

The invention belongs to the technical field of biological carriers, and particularly relates to a preparation method and application of an exosome ferulic acid nano-composite. The invention provides a way for preparing ferulic acid nano-particles by using exosomes, and particularly relates to a preparation process for forming a nano-composite by using ferulic acid and stress yeast exosomes generated by stimulation. The preparation method of the exosome ferulic acid nano-composite provided by the invention has the advantages of high safety, low immunological rejection, high cell fusion degree, high entrapment rate and good stability. Besides, the selected stress yeast exosome can effectively protect and enhance the anti-inflammatory and anti-oxidation effects of ferulic acid, so that the prepared exosome ferulic acid nano-composite has relatively strong anti-inflammatory, anti-oxidation and repair effects, and has a good application prospect in the field of cosmetics or medicines.

The invention relates to the field of preparation of CD19 targeted CAR-T cells, in particular to an adjustable CD19 targeted CAR-T cell preparation technology which comprises the following operation steps: S1, T cell collection and separation: S1.1, peripheral blood collection: blood is collected from peripheral veins of a patient, and the general collection amount is determined according to the physical condition of the patient and the amount required by subsequent cell culture and is usually between 50ml and 500ml; s1.2, performing density gradient centrifugal separation on the T cells; s2, CAR gene construction and carrier preparation: S2.1, CAR structure design (taking an inducible promoter system as an example), CD19 targeting CAR-T cells can accurately recognize and combine CD19 antigens on the surfaces of tumor cells, and as CD19 is highly expressed on the surfaces of most blood system malignant tumor cells such as B cell lymphoma and leukemia and is limited in expression in normal tissue cells, the CD19 targeting CAR-T cells can be used for accurately recognizing and combining the CD19 antigens on the surfaces of the tumor cells. Therefore, the CAR-T cells can specifically attack tumor cells like a'biological missile ', and damage to normal tissues is reduced.

This invention discloses a solution-driven collagen-based artificial muscle, its preparation method, and its application. The preparation method of the artificial muscle includes the following steps: dissolving collagen powder in water, adding acetic acid to swell the solution, and mixing thoroughly to obtain a collagen solution; preparing a coagulation bath; adding the collagen solution to the coagulation bath, and obtaining collagen fibers through wet spinning; subsequently, twisting and shaping the obtained collagen fibers to obtain the solution-driven collagen-based artificial muscle. This invention, by combining wet spinning and twisting techniques, prepares a collagen-based artificial muscle with both excellent mechanical properties and good biocompatibility. This muscle exhibits high flexibility, long cycle life, fast response speed, and high strain. The entire preparation process is simple to operate, and the raw materials are inexpensive and readily available.

The invention provides composite hydrogel for promoting tissue regeneration and repair as well as a preparation method and application of the composite hydrogel, and belongs to the field of hydrogel materials. The method comprises the following steps: carrying out anhydride aminolysis ring-opening reaction on a styrene-maleic anhydride alternating copolymer, amino polyethylene glycol methyl ether, 3-aminophenylboronic acid and cysteamine hydrochloride; the preparation method comprises the following steps: carrying out a first amidation reaction on GRGDS pentapeptide and an SPDP cross-linking agent, then carrying out a disulfide bond exchange reaction on the obtained product and GPLGIAGQ-PEG-SH, and finally carrying out a second amidation reaction on the obtained product and 3-maleimide propionic acid-NHS ester; the preparation method comprises the following steps: carrying out a modification reaction on a PEDOT: PSS aqueous dispersion and 3-aminopropyltriethoxysilane; the preparation method comprises the following steps: mixing a multifunctional polymer, a signal molecule, a conductive component, four-arm PEG-hydrazide and oxidized sodium alginate, and sequentially carrying out physical gelation and chemical crosslinking to obtain the composite hydrogel. Therefore, the intelligent responsiveness, the biological activity and the conductivity of the hydrogel are synergistically improved.

The application discloses an ACVR2A antibody and application thereof, and relates to the technical field of biological medicine. The antibody provided by the application has the advantages of stronger affinity to ACVR2A and higher selectivity to ACVR2A, and provides diversified treatment options for different clinical requirements and expands the application potential of ACVR2A targeted treatment.

The invention relates to the technical field of biological medicines, in particular to an amniotic stem cell preparation and application thereof in treating erectile dysfunction. The preparation method of the amniotic stem cell preparation comprises the following steps: adding amniotic stem cells, the active extract, butanediol, glycerol, trehalose, glucose, amino acid and vitamins into normal saline containing human umbilical cord blood plasma, uniformly mixing to obtain the amniotic stem cell preparation, and storing the amniotic stem cell preparation in a liquid nitrogen tank. The prepared amniotic stem cell can significantly increase the ICP / MAP ratio, improve the activity of NOS and GC, increase the content of NO and cGMP, effectively improve erectile dysfunction and repair damaged tissues.

The invention discloses a preparation method of an antler-DCB bone transplantation scaffold material, which specifically comprises the following steps: S1, cutting the cancellous bone of an antler into a bone sample, and carrying out ultrasonic treatment; s2, soaking in ethanol, and shaking with a shaking table; s3, soaking in diethyl ether, and shaking with a shaking table; s4, soaking the bone sample in a sodium hydroxide solution for shaking table oscillation, cleaning the bone sample with flowing water after soaking, treating the bone sample with hydrogen peroxide with the mass fraction of 30% for 24 hours after cleaning, and cleaning the bone sample again after treatment; and S5, putting the cleaned bone sample into a mixture of chloroform and methanol in a volume ratio of 3: 1 for 1 hour, washing the bone sample with distilled water, and drying at 70 DEG C for 1 day to prepare the antler-DCB bone transplantation scaffold material. The invention also discloses the antler-DCB bone transplantation scaffold material. According to the antler-DCB bone transplantation scaffold material and the preparation method thereof disclosed by the invention, the antler-DCB bone transplantation scaffold material has high porosity, large aperture and specific surface area, and is more beneficial to bone regeneration.

This invention discloses a method for preparing antler-DCB bone graft scaffold material, specifically: S1, cutting cancellous bone from antlers into bone samples and treating them with ultrasound; S2, immersing the samples in ethanol and shaking on a shaker; S3, immersing the samples in ether and shaking on a shaker; S4, immersing the samples in sodium hydroxide solution and shaking on a shaker, then rinsing the bone samples with running water, treating them with 30% hydrogen peroxide for 24 hours, and rinsing them again; S5, placing the rinsed bone samples in a 3:1 mixture of chloroform and methanol for 1 hour, rinsing them with distilled water, and drying them at 70°C for 1 day to obtain the antler-DCB bone graft scaffold material. This invention also discloses the antler-DCB bone graft scaffold material. The antler-DCB bone graft scaffold material and its preparation method disclosed in this invention have high porosity, large pore size, and specific surface area, which are more conducive to bone regeneration.

The invention provides an artificial cornea scaffold and a preparation method thereof, and belongs to the technical field of new material preparation. The preparation process comprises the following steps: preparing the modified krill protein; preparing a polymethyl methacrylate microsphere template; modifying the modified polyacrylic acid with the modified krill protein; preparing an N, N-isopropylacrylamide monomer reaction solution; and preparing the artificial cornea bracket. The material composition of the double-network hydrogel has low cytotoxicity and immunogenicity, stimulation and rejection reaction to eye tissues can be reduced, attachment and growth of cells are facilitated, and the stent is endowed with sufficient strength and toughness by the interpenetrating double-network structure, so that the stent can bear normal physiological pressure and movement of eyes, and the stent has a good application prospect in the field of ophthalmology, ophthalmology, ophthalmology, ophthalmology, ophthalmology, ophthalmology, ophthalmology, ophthalmology, ophthalmology, ophthalmology and ophthalmology. Meanwhile, the stability and the porous characteristic of the structure are kept, so that exchange of nutrient substances and metabolic wastes is facilitated, a good microenvironment is provided for survival and function maintenance of cells, migration, proliferation and differentiation of corneal cells can be guided, and repair and regeneration of damaged corneal tissues are facilitated.

The invention provides a universal induced pluripotent stem cell in which an HLA-A gene, an HLA-B gene, a CIITA gene and a CD58 gene are knocked out as well as a preparation method and application of the universal induced pluripotent stem cell, a comprehensive immune escape mechanism is realized, the problem of dual attacks of T cells and NK cells in a transplantation process is solved, and the survival rate and clinical feasibility of transplanted cells are improved. The CRISPR-Cas9 system is introduced into the iPSCs by adopting a one-time electrotransfection technology, so that synchronous knockout of four genes is realized, the editing efficiency is greatly improved, and the success rate of simultaneous knockout of a plurality of genes is ensured. The method shortens the cell culture period, is suitable for large-scale application, and reduces the cost and complexity. The iPSCs can be further differentiated into various functional cells, such as mesenchymal stem cells and dopaminergic neurons, while low immunogenicity is kept, and the iPSCs are suitable for various scenes such as tissue engineering, cell drug preparation and transplantation drug preparation.

The invention discloses a 3D printing cavernous body organ-like model capable of simulating an erectile physiological process, and belongs to the technical field of tissue engineering and regenerative medicine. The hydrogel is formed by photo-curing 3D printing of a dual-network hydrogel material formed by blending a pig cavernous body acellular extracellular matrix pregel solution and polyethylene glycol diacrylate, and has a bionic sine curve expansion structure; micron-sized grooves for guiding the directional arrangement of smooth muscle cells are formed in the surface of the micro-sphere, and an elliptical cavity for providing structural support is formed in the micro-sphere. A pig cavernous body dECM pre-gel solution is used as a base material, so that a microenvironment for living of cells in a body can be effectively simulated; by regulating and controlling the proportion of PEGDA, the mechanical property of the model is matched with that of a natural cavernous body tissue. The organ model can reproduce the NO-mediated smooth muscle cell relaxation and tissue expansion physiological process in vitro, and provides an effective in-vitro platform for researching the pathological mechanism of erectile dysfunction, screening drugs and developing a novel treatment method.

The invention provides a medical rubber composite material as well as a preparation method and application thereof, and relates to the technical field of medical material engineering. The medical rubber composite material is prepared from the following components: rubber, polyether-ether-ketone, a hydroxyapatite biological compatibilizer, a bacteriostatic agent and a cross-linking agent. The preparation method comprises the following steps: (1) preparing rubber fibers; and (2) mixing the rubber fibers in the step (1) with polyether-ether-ketone, a biological compatilizer, a bacteriostatic agent and a cross-linking agent to obtain a rubber-based solution. Stirring the rubber-based solution, and precipitating to separate out a polymer; and (3) carrying out freeze drying treatment on the polymer obtained in the step (2) to obtain the medical rubber composite material. According to the application of the rubber medical composite material obtained by adopting the components and the preparation method in preparation of rubber-based composite aerogel, the obtained rubber-based aerogel is simple to operate, low in manufacturing cost, high in porosity, adjustable in mechanical strength and good in biocompatibility and antibacterial property.

The invention belongs to the field of medical materials, and particularly relates to an acellular dermal matrix and a cross-linking modification method thereof. According to the method, the acellular dermal matrix (ADM) is subjected to cross-linking modification by adopting glutamine transaminase (TG enzyme) in a mild and easy-to-operate manner, so that the physical and mechanical properties, the biocompatibility and the biological activity of the acellular dermal matrix (ADM) are improved. The thermal denaturation temperature, the thermal shrinkage temperature, the tensile strength and the like of the ADM dressing modified by glutamine transaminase (TG enzyme) are remarkably improved, and the ADM dressing has low immunogenicity and controllable degradability. The method can be used for preparing high-end dressings, hemostatic membrane materials, biological tissue patches, soft tissue repair materials, tissue engineering scaffold materials and the like, and has wide clinical application prospects.

This invention belongs to the field of antibody technology, specifically relating to an IL13Rα2 nanobody targeting IL13Rα2, a chimeric immune receptor, and their applications and products. This invention is the first to discover an IL13Rα2 nanobody with a specific complementarity-determining region amino acid sequence. The nanobody possesses advantages such as high affinity, high specificity, low immunogenicity, small molecular weight, and strong tissue penetration. This invention also provides a chimeric immune receptor comprising the nanobody, recombinant protein, nucleic acid molecule, expression vector, engineered immune cells, antibody preparation, kit, and pharmaceutical composition. CAR-T cells constructed based on the nanobody exhibit highly efficient and specific killing ability against IL13Rα2-positive tumor cells and can effectively overcome tumor antigen heterogeneity. They can be used to prepare drugs or kits for the diagnosis or treatment of IL13Rα2-related diseases, showing promising clinical application prospects.

The present application relates to the technical field of nanobody, in particular to anti-FcRH5 nanobody and its application. The present application provides a kind of anti-FcRH5 nanobody with high affinity and specificity, which is based on the single domain heavy chain variable region structure of camelid origin, with the advantages of small molecular weight, high stability, strong tissue penetration, easy to be engineered and the like. The nanobody specifically recognizes and binds to the target point highly expressed and stably expressed on the surface of multiple myeloma cells through its complementarity determining region. The nanobody of the present application can be used as a core recognition element to construct a variety of therapeutic or diagnostic tools such as chimeric antigen receptor T cells, bispecific antibodies, antibody drug conjugates or immunodetection probes. The technical scheme can solve the technical problem that there is a lack of high-quality, functionally clear anti-FcRH5 antibody in the prior art. The nanobody of the present application has significant clinical transformation and industrialization advantages, and promotes the treatment of multiple myeloma to multiple target points.

The application belongs to the field of biomedical materials, and particularly relates to a self-polarization degradable gradient electroactive hydrogel as well as a preparation method and application thereof. The application develops a novel degradable electroactive scaffold based on a high-electroactive natural macromolecule hydrogel, and through material component regulation and control and structure design, the piezoelectric performance of the scaffold is significantly improved, and the synergistic precise regulation and control of the scaffold on bone and cartilage regeneration are realized. Through the combination of electron transfer and ion transfer, the pathological electrical requirements of bone and cartilage regions are respectively adapted, and through the differential regulation and control of the electrical microenvironment and the material structure, the efficient bionics and bidirectional repair of the bone-cartilage interface are realized.

The application discloses a method for preparing a silk fibroin and hyaluronic acid composite gel, which comprises the following steps: uniformly mixing hyaluronate with a first solvent to prepare a hyaluronic acid gel, mixing the hyaluronic acid gel with a silk fibroin solution, high-speed shearing homogenization and emulsification, standing, high-speed shearing homogenization and emulsification, sealing, sterilization, and obtaining the composite gel. The method does not need to add additional cross-linking agents, initiators, enzymes and other reagents; during the homogenization and gel formation process, the silk fibroin solution and the hyaluronic acid gel can form a uniform mutual cross-linking double network, the risk of phase separation is low, and high-speed homogenization can make the silk fibroin produce an emulsification reaction, so that the color of the composite gel is whiter; the preparation method is simple, fast emulsification and gel formation, the production cycle is short, the product has low endotoxin, high biocompatibility, slow degradation, small inflammatory reaction, high safety of injection and filling, and the composite gel can play a role in water replenishment and moisturization after subcutaneous injection, has the effects of improving skin elasticity and gloss, and lightening fine lines and other whitening effects.

This invention specifically discloses an ACVR2A antibody and its applications, relating to the field of biomedical technology. The antibody provided by this invention has a stronger affinity for and higher selectivity for ACVR2A, offering diverse treatment options for different clinical needs and expanding the application potential of ACVR2A-targeted therapy.