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Biological ink for 3D printing of artificial skin

A technology of bio-ink and artificial skin, applied in drug delivery, medical science, capsule delivery, etc., can solve the problems of immune rejection, bacterial contamination, incomplete differentiation of exogenous stem cells, etc., and achieve the goal of reducing immune rejection Effect

Inactive Publication Date: 2020-02-11
THE THIRD XIANGYA HOSPITAL OF CENT SOUTH UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although through 3D scanning, it is possible to print a fully customized skin that conforms to specific parts of the human body and specific 3D features, which improves the fit and success rate of artificial skin and wounds, but the stem cell extraction does not take into account immune rejection, exogenous stem cells, etc. not fully differentiated
(2) The invention patent "A Method for Constructing Skin Tissue Based on Bio-3D Printing" (Publication No. CN106860918A) mentioned that the artificial skin using collagen sponge structure can shorten the wound healing period and enhance the skin elasticity after wound healing, but in In case of bacterial contamination during printing

Method used

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  • Biological ink for 3D printing of artificial skin
  • Biological ink for 3D printing of artificial skin
  • Biological ink for 3D printing of artificial skin

Examples

Experimental program
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Effect test

preparation example Construction

[0049] A preparation method for bioink for 3D printing artificial skin, has the following steps:

[0050] S1: Enzymatic solution configuration: D-Hanks solution was used to prepare 2g / L neutral protease solution, trypsin with a mass fraction of 0.25%, and EDTA with a mass fraction of 0.02%, and stored at 4°C after filter sterilization.

[0051] S2: Preparation of type IV collagen culture bottle: Dissolve human type IV collagen in 0.1% acetic acid solution, filter and sterilize, and store at 4°C for later use. Spread 1-2ml of the prepared type IV collagen in a culture bottle overnight, discard the supernatant, rinse 3-4 times with PBS solution with a concentration of 0.01mol / L, dry in a 40°C drying oven, and irradiate with ultraviolet light 2h disinfection standby.

[0052] S3: Extraction of epidermal stem cells: take normal skin slices from the body, wash them with PBS solution containing double antibiotics (penicillin, streptomycin) for 5-6 times, and remove the subcutaneous...

Embodiment 1

[0078] A kind of bio-ink for 3D printing artificial skin, the preparation of its C component and decellularized matrix scaffold comprises the following steps:

[0079] ①Dissolve gelatin in PBS buffer to form a 10wt% solution; dissolve sodium alginate in PBS buffer to form a 2wt% solution; use NaOH to neutralize residual acetic acid, adjust the pH value to 7.2, and batch in a 60°C oven Sterilize.

[0080] ② Weigh a certain amount of type I Sigma collagen powder, add acetic acid to a final concentration of 30 mg / mL, stir, and put it in a 4°C refrigerator overnight after stirring.

[0081] ③ According to the mass ratio of gelatin solution: collagen solution: sodium alginate solution is 1:1:2, mix evenly and carry out high temperature sterilization.

[0082] ④ Use 3D modeling software to reconstruct the image of the constructed 3D model to generate an STL file, import it into the 3D bioprinter for layering and setting of printing parameters, and then start printing.

[0083] ⑤ A...

Embodiment 2

[0085] A kind of bio-ink for 3D printing artificial skin, the preparation of its C component and decellularized matrix scaffold comprises the following steps:

[0086] ① Dissolve gelatin in PBS buffer to form a 10% solution, dissolve sodium alginate in PBS buffer to form a 2% solution, neutralize residual acetic acid with NaOH, adjust the pH value to 7.2, and batch in an oven at 60°C Sterilize.

[0087] ② Weigh a certain amount of type I Sigma collagen powder, add acetic acid to a final concentration of 30 mg / mL, stir, and put it in a 4°C refrigerator overnight after stirring.

[0088] ③ According to the mass ratio of gelatin solution: collagen solution: sodium alginate solution is 1:1:4, mix evenly and carry out high temperature sterilization.

[0089] ④ Use 3D modeling software to reconstruct the image of the constructed 3D model to generate an STL file, import it into the 3D bioprinter for layering and setting of printing parameters, and then start printing.

[0090] ⑤ Af...

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Abstract

The invention relates to biological ink for 3D printing of artificial skin, and belongs to the technical field of tissue engineering. The biological ink comprises a component A used for constructing an epidermal layer, a component B used for constructing a corium layer and a component C used for constructing an acellular matrix scaffold, wherein the component A comprises first seed cells, first carrier hydrogel and first growth factor slow-release gel containing traditional Chinese medicine components, and the mass ratio of the first carrier hydrogel to the first growth factor slow-release gelis (60: 1)-(60: 8); and the component B comprises second seed cells, second carrier hydrogel and second growth factor slow-release gel. The traditional Chinese medicine components such as dragon's blood and ampelopsis japonica and growth factor slow-release gel are added, and seed cells are extracted from the skin of a patient. The biological ink has good biocompatibility, can effectively promotecell proliferation and regeneration and wound healing, has good mechanical and fluidic properties, and can be used in 3D printing skin and other related fields.

Description

technical field [0001] The invention relates to a biological ink for 3D printing artificial skin. Background technique [0002] In China, 26 million people suffer from various degrees of burns and scalds every year. Among burn patients, 49% are disabled and 8% are permanently disabled. For the treatment of general I and II degree burns, it is mainly to smear scald ointment and take antibiotics. However, for patients with third-degree burns and deep second-degree burns whose wounds cannot heal by themselves, skin grafting is required. According to statistics, there are more than 3.2 million patients who need skin transplantation every year, and about 200,000 patients need to use skin replacement materials in a large area, with an average area of ​​5000 cm per person according to international statistics. 2 Calculated, the total required amount is nearly 109cm 2 . However, due to limited medical technology, large area of ​​skin grafting, high price and other reasons, autol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60A61L27/36A61L27/38A61L27/48A61L27/54A61L27/26
CPCA61L27/60A61L27/3834A61L27/362A61L27/26A61L27/48A61L27/54A61L2300/414A61L2300/30A61L2300/622A61L2300/412A61L2300/404C08L89/00C08L5/04
Inventor 刘雨辰谢文韬李晓茹杨慧洁谢媛媛
Owner THE THIRD XIANGYA HOSPITAL OF CENT SOUTH UNIV
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