Glutathione-modified chitosan copolymer serving as non-viral gene carrier material and preparation and application thereof

A technology of chitosan copolymer and glutathione is applied in the field of preparation of non-viral gene carriers, which can solve the problems of low transfection rate of active genes, low-efficiency intracellular operation, unfavorable biological distribution, etc., and reduce cytotoxicity. , low immune rejection, good protective effect
CN102140171AActive Publication Date: 2011-08-03NANKAI UNIV
2 Cites 22 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANKAI UNIV
Publication Date
2011-08-03

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a glutathione-modified chitosan copolymer serving as a non-viral gene carrier material and preparation and application thereof, which belong to the fields of gene therapy and novel materials. A preparation method of the copolymer comprises the following steps of: (1) synthesizing an allyl-modified chitosan derivative; (2) synthesizing brush-like PEG (Polyethylene Glycol) polymer chains with different molecular weights through RAFT (Reversible Addition-Fragmentation Chain Transfer); (3) grafting the brush-like PEG onto a chitosan framework by adopting a free radical coupling method; and (4) linking glutathione to the chain end of the brush-like PEG by adopting an EDC (1-Ethyl-3-(3-Dimethyllaminopropyl) Carbodiimide hydrochloride) / NHS (N-hydroxysuccinimide) activation method to obtain a glutathione-modified chitosan copolymer carrier material. The glutathione-modified chitosan copolymer obtained by adopting the technology serves as the non-viral gene carrier material. By adopting the copolymer, the endocytosis function of a composite nanoparticle formed from the copolymer and DNA (Deoxyribonucleic Acid) can be remarkably enhanced, the releasing mechanism of DNA from a composite particle after cell entrance is improved, and the non-viral gene carrier material with a high transfection efficiency is further obtained.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of gene therapy and new materials, in particular to a preparation method of a non-viral gene carrier based on glutathione modified chitosan. Background technique

[0002] Gene therapy has attracted increasing attention in the treatment of various human diseases. Gene therapy is a technology that uses molecular biology methods to introduce a target gene into a patient to express the target gene product, so that the disease can be treated. The carrier problem has always been one of the core technologies in the field of gene therapy research, and the key technology of gene therapy is the selection of gene delivery carrier. Gene vectors are usually divided into viral vectors and non-viral vectors. In most cases, viral vectors have high transfection efficiency, but there are problems such as toxicity, immunogenicity and gene transfection targeting, which limit their application in clinical treatment. Although non-viral v...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More