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48results about How to "High dissociation efficiency" patented technology

Glutathione-modified chitosan copolymer serving as non-viral gene carrier material and preparation and application thereof

The invention discloses a glutathione-modified chitosan copolymer serving as a non-viral gene carrier material and preparation and application thereof, which belong to the fields of gene therapy and novel materials. A preparation method of the copolymer comprises the following steps of: (1) synthesizing an allyl-modified chitosan derivative; (2) synthesizing brush-like PEG (Polyethylene Glycol) polymer chains with different molecular weights through RAFT (Reversible Addition-Fragmentation Chain Transfer); (3) grafting the brush-like PEG onto a chitosan framework by adopting a free radical coupling method; and (4) linking glutathione to the chain end of the brush-like PEG by adopting an EDC (1-Ethyl-3-(3-Dimethyllaminopropyl) Carbodiimide hydrochloride)/NHS (N-hydroxysuccinimide) activation method to obtain a glutathione-modified chitosan copolymer carrier material. The glutathione-modified chitosan copolymer obtained by adopting the technology serves as the non-viral gene carrier material. By adopting the copolymer, the endocytosis function of a composite nanoparticle formed from the copolymer and DNA (Deoxyribonucleic Acid) can be remarkably enhanced, the releasing mechanism of DNA from a composite particle after cell entrance is improved, and the non-viral gene carrier material with a high transfection efficiency is further obtained.
Owner:NANKAI UNIV

Short process purification method of graphite

The invention relates to a short process purification method of graphite. The method includes crushing, grinding and flotation steps, and is characterized in that the method includes the following steps: (1) preparing graphite or a carbonaceous material having a particle size of less than 1 mm and a fixed carbon content of not less than 35% into pulp having a concentration of not higher than 60%,placing a direct contact type ultrasonic oscillator into the pulp, and controlling the power to be not less than 1.0 kW and time to be not less than 1 min; (2) diluting the crushed and dispersed pulpto pulp having a concentration of not higher than 30% and sending the diluted pulp into flotation equipment, adding an inhibitor, a collector and a foaming agent for coarse selection and fine selection in order, and carrying out stirring, inflation and flotation to obtain graphite concentrate and graphite tailings; and (3) condensing the graphite concentrate and graphite tailings until the concentration is 30%-60% or sending the graphite concentrate and graphite tailings into a direct contact type ultrasonic oscillator container for crushing and dispersing, and repeating the step (1) and (2) for 2-3 times. The beneficial effects of the short process purification method of graphite are that the method improves the dissociation efficiency, reduces the number of grinding and flotation, helpsto reduce the amount of a flotation reagent and improve the efficiency of the flotation reagent, optimizes the flotation effect, and improves the carbon content of the concentrate.
Owner:(CNBM) BENGBU DESIGN & RES INST FOR GLASS IND CO LTD

Automatic detaching machine for abandoned electronic circuit board element

The invention relates to an automatic detaching machine for an abandoned electronic circuit board element and belongs to the technical field of metallurgy and chemical industry. The automatic detaching machine comprises a horizontal shell, wherein a feeding port is formed at the front end of the shell; a rotary furnace and a rotary screen are respectively arranged in the shell along the horizontal direction according to a sequence; a discharging port is arranged at the rear end of the rotary furnace; a transmission mechanism is respectively connected with the exteriors of the rotary furnace and the rotary screen; the rotary screen comprises a first screen, a second screen and a third screen, the meshes of which are increasingly big; a tin solder blanking port is arranged under the first screen; an element blanking port is arranged under the second screen; a plate blanking port is arranged on the third screen; a substrate blanking port is arranged under the third screen; a nozzle passes through the rear end of the shell; a connecting fan is fixed in the shell; an air pipe is connected with the fan; and a thermodetector is arranged on the upper part of a superposed area of the rotary furnace and the rotary screen on the shell. The automatic detaching machine provided by the invention is simple in operation, strong in adaptability to circuit boards in different sizes, low in production cost and higher in processing capacity.
Owner:YANGZHOU NINGDA NOBLE METAL CO LTD

Glutathione-modified chitosan copolymer serving as non-viral gene carrier material and preparation and application thereof

The invention discloses a glutathione-modified chitosan copolymer serving as a non-viral gene carrier material and preparation and application thereof, which belong to the fields of gene therapy and novel materials. A preparation method of the copolymer comprises the following steps of: (1) synthesizing an allyl-modified chitosan derivative; (2) synthesizing brush-like PEG (Polyethylene Glycol) polymer chains with different molecular weights through RAFT (Reversible Addition-Fragmentation Chain Transfer); (3) grafting the brush-like PEG onto a chitosan framework by adopting a free radical coupling method; and (4) linking glutathione to the chain end of the brush-like PEG by adopting an EDC (1-Ethyl-3-(3-Dimethyllaminopropyl) Carbodiimide hydrochloride) / NHS (N-hydroxysuccinimide) activation method to obtain a glutathione-modified chitosan copolymer carrier material. The glutathione-modified chitosan copolymer obtained by adopting the technology serves as the non-viral gene carrier material. By adopting the copolymer, the endocytosis function of a composite nanoparticle formed from the copolymer and DNA (Deoxyribonucleic Acid) can be remarkably enhanced, the releasing mechanism of DNA from a composite particle after cell entrance is improved, and the non-viral gene carrier material with a high transfection efficiency is further obtained.
Owner:NANKAI UNIV

Organic small-molecule photovoltaic device based on three-terminal electrode structure

The invention belongs to the field of organic photovoltaic cells, and particularly relates to an organic small-molecule photovoltaic device based on a three-terminal electrode structure. The organic small-molecule photovoltaic device comprises an ITO layer serving as a first electrode layer, an organic small-molecule donor connected with a conductive face of the ITO layer, an organic small-molecule receptor connected with the other face of the organic small-molecule donor, a second electrode layer connected with the other face of the organic small-molecule receptor, a dielectric layer connected with the other face of the second electrode layer, and a third electrode layer connected with the other face of the dielectric layer, the first electrode layer is connected with a negative electrode of a direct-current voltage source, the third electrode layer is connected to a positive electrode of the direct-current voltage source, and the first electrode layer and the second electrode layer serve as output ends. According to the device, due to the fact that a potential is applied to the two ends of the organic small-molecule photovoltaic device, a strong electric field can be formed in the organic small-molecule photovoltaic device, the exciton disassociation efficiency is improved, and the energy conversion benefit of the organic small-molecule photovoltaic device is improved.
Owner:TAIYUAN UNIV OF TECH

Preparation method of nano bismuth/graphene composite material and application of nano bismuth/graphene composite material in MALDI-MS

The invention relates to the technical field of analysis, in particular to a preparation method of a nano bismuth/graphene composite material and the application of the nano bismuth/graphene composite material in MALDI-MS. The preparation method comprises the following steps of S01, preparing GO, and preparing a GO aqueous solution with the concentration being 0.5 mg/mL to 1.5 mg/mL; S02, adding bismuth salt in the step S01, stirring for 20-40 minutes, and adjusting the pH of the solution to be 9-10; S03, putting the solution treated in the step S02 into a reaction kettle to react for 8-12 hours; and S04, cleaning the solid obtained by the reaction in the step S03 with ethanol and water for multiple times, and then carrying out vacuum drying to obtain the bismuth/graphene composite material. According to an MALDI-MS negative ion mode analysis method established based on the bismuth-based nano material, the electron distribution in the system can be influenced by constructing the composite material, so that light absorption of the composite material is increased, separation of electron-hole pairs is accelerated, and the dissociation efficiency of an object to be detected is improved.
Owner:广东省农业科学院农业质量标准与监测技术研究所 +1

Reagent kit and method for dissociating animal embryo

The invention discloses a reagent kit and method for dissociating an animal embryo. The reagent kit for dissociating an animal embryo disclosed by the invention comprises reagents of which the names are respectively an enzymolysis solution C, an enzymolysis solution D and an enzymolysis solution E, wherein the enzymolysis solution C consists of cytochalasin storing fluid and TrypLE digestive fluid, and the volume ratio of the cytochalasin storing fluid to the TrypLE digestive fluid is (3 to 47) to (3 to:97); the enzymolysis solution D is fluid consisting of fetal calf serums, a pronase solution and a TCM-HEPES buffer solution, and the volume ratio of the fetal calf serums to the pronase solution to the TCM-HEPES buffer solution is (1 to 1 to 1) to (1 to 2 to 1); and the enzymolysis solution E consists of cytochalasin storing fluid and Accutase cell separation fluid, and the volume ratio of the cytochalasin storing fluid to the Accutase cell separation fluid is (3 to 47) to (3 to 97). Experiment proves that when the reagent kit and method disclosed by the invention are used for dissociating the animal embryo, the dissociating efficiency is high, the embryo of an animal of species, which cannot be dissociated by an existing method, can be dissociated, the reagent kit and the method are suitable for the embryos at different developmental stages, and hurt to cells in the dissociating process can also be reduced.
Owner:SHENZHEN HUADA GENE INST

Analysis and detection method for effectively screening phospholipid in krill oil

The invention discloses an analysis and detection method for effectively screening phospholipid in krill oil. The method comprises the following steps: taking krill powder, uniformly grinding, addinginto a trichloromethane-methanol mixed solution, and uniformly oscillating at room temperature to obtain an extracting solution A; performing ultrasonic treatment on the extracting solution A, centrifuging the mixture, and collecting a lower-layer organic phase and centrifuged residues to obtain an organic phase B and residues C; re-extracting the residue C with trichloromethane for multiple times, and collecting a lower-layer organic phase to obtain an organic phase D; mixing the organic phase B with the organic phase D to obtain an e1 extracting solution, blow-drying the e1 extracting solution, fixing the volume with acetonitrile, and filtering with an organic filter membrane to obtain a to-be-detected solution E; and finally, detecting the to-be-detected solution E through a hydrophilicinteraction liquid chromatography-mass spectrometry method in a precursor ion scanning mode. The method can realize non-targeted screening of phospholipid in krill oil, and has the characteristics ofhigh detection efficiency, high detection accuracy and high resolution.
Owner:ZHEJIANG GONGSHANG UNIVERSITY
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