Cationic polymer as well as preparation method and application thereof

A cationic polymer and polymer technology, applied in the direction of using vectors to introduce foreign genetic material, recombinant DNA technology, etc., can solve problems such as transfection failure and cytotoxicity
CN102464801AInactive Publication Date: 2012-05-23SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
Publication Date
2012-05-23
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a novel cationic polymer used as a non-viral gene carrier as well as a preparation method and application thereof. The cationic polymer provided by the invention has a structure shown as the following formula I. The cationic polymer has a 1,2,3-triazole group structure and a disulfide bond structure and is generated by an alkynylation monomer and an azide monomer (an azide-diethylene glycol monomer and an azide monomer containing a disulfide bond) through cycloaddition and polymerization of alkynyl-azide catalyzed by single-charge copper ions. The polymer provided by the invention has a structure in which triazole is adjacent to amide, and the structure has specific binding ability with nucleic acid, thus, the stability of genes in the transmission process can be improved; and the disulfide bond contained in the structure also has reduction responsiveness and can be degraded in a cell to further increase the transfection efficiency and lower the toxicity, therefore, the non-viral gene carrier prepared by using the polymer has the characteristics of high biocompatibility, low toxicity, high transfection efficiency and the like.
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Description

technical field

[0001] The present invention relates to a novel cationic polymer, its preparation method and use, and also relates to the nanocomposite composed of the novel cationic polymer and nucleic acid, its preparation method and use. Background technique

[0002] Gene therapy refers to the introduction of normal genes or genes with therapeutic effects into target cells in a certain way to correct gene defects or exert therapeutic effects. To be precise, it is the transfer of specific nucleic acid sequences related to genetic information. It is a comprehensive Sexually difficult biotechnology. Therefore, the key issue of gene therapy is to find highly efficient and low-toxic gene carriers. The gene vectors researched and developed so far mainly include viral vectors and non-viral vectors. Among them, viral vectors, such as various retroviruses, adenoviruses, herpes viruses, and adeno-associated viruses, have high transfection efficiency, but there are There are many ...

Claims

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