Preparation method of bionic artificial nerve scaffold established by collagen

A collagen and artificial nerve technology, applied in the field of bionic artificial nerve scaffold preparation, can solve the problems of host immune rejection, easy collapse, complicated material extraction and purification, etc., and achieve the effect of low immune rejection and high mechanical strength of degradable

Active Publication Date: 2015-05-27
广州南方医大科技园有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] A variety of nerve replacement materials have been found so far, including biological materials such as: autologous tissue blood vessels? sarcolemma? amniotic tube; allogeneic tissue blood vessels? Reactive or easy to collapse, material collection and purification are more complicated, and the length, diameter, wall thickness and other physical properties of the catheter cannot be precisely controlled according to the needs, which limits its clinical application? At present, biodegradable materials are more researched at home and abroad. ? Among them, collagen has been widely used because of its strong plasticity, low immune rejection, and convenient acquisition. However, the collagen materials reported so far cannot Good for guiding the growth of regenerative nerves

Method used

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  • Preparation method of bionic artificial nerve scaffold established by collagen
  • Preparation method of bionic artificial nerve scaffold established by collagen
  • Preparation method of bionic artificial nerve scaffold established by collagen

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Experimental program
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Effect test

Embodiment 1

[0033] 1) Prepare a collagen solution with a concentration of 20mg / ml (made from commercially available collagen powder and water), pour it into a mold and freeze at -20°C for 1 hour, and then put it in a freeze dryer vacuum-70°C for 20 minutes ; Take out the columnar collagen material with a length of 1cm and a diameter of 1mm;

[0034] 2) The above-mentioned collagen material is slowly stretched to twice its original length at a speed of 1.2mm per second (drawing equipment) (the existing equipment on the market, and the American Sutter Instrument P97 programmable horizontal microelectrode drawing instrument can be used). Schematic diagram such as figure 1 As shown), the specific change process of collagen material in the stretching process is as follows figure 2 As shown in A~C, then freeze together with the equipment at -20°C for 1 hour, and then place it in vacuum -70°C for a second freeze-drying for 2 hours to obtain a linear scaffold with orderly microtubes inside (that is,...

Embodiment 2

[0043] 1) Preparation of ordered collagen scaffold: prepare a collagen solution with a concentration of 25mg / ml, pour it into a mold, freeze at -15°C for 80 minutes, and then put it in a freeze dryer vacuum -60°C for 25 minutes; take it out to be 1.2cm long Columnar collagen material with a diameter of 1.2mm;

[0044] 2) The above-mentioned collagen material is slowly stretched to 2.5 times the original length at a speed of 1.4mm per second with a drawing equipment, and then in the stretched state, it is frozen at -15°C for 80 minutes, and then put in a vacuum -80 Perform a second freeze-drying at ℃ for 1.5 hours to obtain a linear scaffold containing ordered micropipes inside, that is, an ordered collagen scaffold, ready for use;

[0045] 3) Preparation of nanofiber film: dissolve the collagen powder with hexafluoroisopropanol, and the concentration of collagen in the solution obtained is 80mg / ml. The solution is made into a nanofiber film with a thickness of 2.2mm by electrospinn...

Embodiment 3

[0049] 1) Preparation of ordered collagen scaffold: prepare a collagen solution with a concentration of 15mg / ml, pour it into a mold and freeze at -25°C for 60 minutes, then put it in a freeze dryer and freeze-dry at -80°C for 15 minutes; take it out to a length of 0.8cm Columnar collagen material with a diameter of 0.8mm;

[0050] 2) The above-mentioned collagen material is slowly stretched to 1.5 times the original length at a speed of 1mm per second with a drawing equipment, and then in the stretched state, it is placed at -25°C and frozen for 60 minutes, and then placed in a vacuum at -60°C Perform a second freeze-drying for 2.5 hours to obtain a linear scaffold containing ordered micropipes inside, that is, an ordered collagen scaffold, ready for use;

[0051] 3) Preparation of nanofiber film: dissolve the collagen powder with hexafluoroisopropanol, and the concentration of collagen in the solution obtained is 120mg / ml. The solution is made into a nanofiber film with a thickne...

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Abstract

The invention discloses a preparation method of a bionic artificial nerve scaffold established by a collagen. The method comprises the following steps: firstly preparing the collagen in three different shapes for later use: 1) dissolving the frozen and dried collagen by using hexafluoroisopropanol and preparing a nanofiber thin film by using an electrospinning technique; 2) preparing a linear scaffold containing an ordered fine pipeline by the collagen by combining freezing and drying with a mechanical stretching technology; and 3) dissolving the collagen in pure water to prepare a hydrogel; then, coiling the nanofiber thin film into a tube to simulate perilemma, wherein the ordered pipeline linear scaffold is filled in the tube to simulate perineurium; and finally, filling the hydrogel in the pipeline of the linear scaffold and the gap of the scaffold to simulate endoneurium. The bionic artificial nerve scaffold based on a bionic theory better simulates the structure of peripheral nerves and can promote and guide axon and Schwann cells to grow orderly and pass through damaged peripheral nerves based on a good mechanical property.

Description

Technical field [0001] The present invention is a preparation area for organizational engineering artificial neuroscopy, which specializes in a preparation method of bionic artificial neuropathy built by collagen. Background technique [0002] With the development of modern society, accident damage? Earthquake? Tumor surgery, etc., increased the number of nerve injuries year by year. Among the new trauma cases worldwide in the world, there are about 1.5%to 4.0%of surrounding nerve injury cases?There are 600,000 to 900,000 cases per year, and there are about 300,000 to 450,000 cases that need to be used to repair the nerve through nerve transplantation.Rebuilding is a major problem in the current trauma surgery? Micro -surgery? With the development of tissue engineering, peripheral nerve tissue engineering has also gradually developed. It is recognized as one of the effective means to solve this problem? Neurot catheters are many scholars in many scholars inBased on the previous r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/50
Inventor 郭家松王祥海
Owner 广州南方医大科技园有限公司
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