The invention relates to the technical field of cell biology, and in particular to a naked mole rate Schwann cell separation & purification and culture method. According to the method provided by the invention, Schwann cells are separated & purified and cultured from marrow DRG (dorsal root ganglion) of a fetal naked mole rate with the comprehensive use of a plurality of mediums, so that a reasonable method suitable for culturing the Schwann cells of a heterothermic mammal of rodent species, namely the naked mole rate, is found out. With the application of the method provided by the invention, a great amount of naked mole rate Schwann cells, which are normal in functions and activity, are conveniently, efficiently and economically obtained; the cells, when cultured under a hypoxia condition, can still reserve biological characteristics under an in-vivo state in an in-vitro environment, so as to facilitate further research, which is directly conducted in a pure in-vitro cell culture model, on special physiological functions of the naked mole rate Schwann cells; therefore, important theoretical bases are provided for researching a biological mechanism therein and for application to related clinical fields.