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Allograft nerve graft, the method for preparing the same and uses thereof

A technology of grafts and nerves, applied in the medical field, can solve problems such as surgical failure, little clinical application value, and different structural nerve basement membranes

Inactive Publication Date: 2008-01-02
GENERAL HOSPITAL OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Biological materials such as autologous skeletal muscle and autologous vein, and artificial synthetic materials such as silicone tube, alginate gel, PGA, PLA, etc. have been studied as alternative materials, but their structure is very different from that of nerve basement membrane. little application value
Fresh allogeneic nerve transplantation has a relatively wide range of sources without sacrificing autologous nerves, but because of immune rejection, it often leads to surgical failure

Method used

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  • Allograft nerve graft, the method for preparing the same and uses thereof
  • Allograft nerve graft, the method for preparing the same and uses thereof
  • Allograft nerve graft, the method for preparing the same and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] 1 Source of material: Human ulnar nerve (donor who died of acute craniocerebral injury).

[0049] 2 preparation process:

[0050] 1) Under sterile conditions, remove epineurium fat and connective tissue under a microscope;

[0051] 2) At 25°C, shake and soak in distilled water on a shaker for 24 hours;

[0052] 3) Shaking, soaking and rinsing in the extract 1 for 24 hours [the preparation method of the extract 1 is as follows: dissolving SB-10 (purchased from Sigma Company) in 0.01M PBS solution to prepare a 250 mM SB-10 solution];

[0053] 4) Rinse with 0.1M PBS solution for 30 minutes;

[0054]5) Shaking and washing in the extract 2 for 24h [the preparation method of the extract 2 is as follows: Dilute Triton X-200 (purchased from Sigma Company) to a concentration of 0.28% in 0.01M PBS, and simultaneously dilute SB-16 (purchased from Sigma Company) ) was dissolved in this solution to prepare 1.2mM of SB-16];

[0055] 6) Repeat steps 3), 4) and 5) in sequence once;...

Embodiment 2

[0060] 1 Source of material: Human ulnar nerve (donor who died of acute craniocerebral injury).

[0061] 2 preparation process:

[0062] 1) Under sterile conditions, remove epineurium fat and connective tissue under a microscope;

[0063] 2) At 10°C, shake and soak in distilled water on a shaker for 28 hours;

[0064] 3) Shaking, soaking and rinsing in the extract 1 for 30 hours (the preparation method of the extract 1 is as follows: dissolve SB-10 in 0.01M PBS solution to prepare a 125mM SB-10 solution);

[0065] 4) Wash with 0.1M PBS solution for 40 minutes

[0066] 5) Shake and wash in the extract 2 for 30 hours (the preparation method of the extract 2 is as follows: Dilute Triton X-200 in 0.01MPBS to a concentration of 0.14%, and dissolve SB-16 in this solution to prepare 0.6mM SB -16);

[0067] 6) Repeat steps 3), 4) and 5) in sequence once;

[0068] 7) Wash the human ulnar nerve with 0.01 MPBS solution for 40 minutes to obtain the product.

[0069] The nerve allogr...

Embodiment 3

[0073] 1 Source of material: Human ulnar nerve (donor who died of acute craniocerebral injury).

[0074] 2 preparation process:

[0075] 1) Under sterile conditions, remove epineurium fat and connective tissue under a microscope;

[0076] 2) Shake, soak and rinse in distilled water on a shaker at 40°C for 12 hours;

[0077] 3) Shaking, soaking and rinsing in the extract 1 for 16 hours (the preparation method of the extract 1 is as follows: dissolve SB-10 in 0.01M PBS solution to prepare a 375mM SB-10 solution);

[0078] 4) Rinse with 0.01M PBS solution for 30 minutes

[0079] 5) Shake and wash in the extract 2 for 16 hours (the preparation method of the extract 2 is as follows: Dilute Triton X-200 in 0.01MPBS to a concentration of 0.42%, and dissolve SB-16 in this solution to prepare 1.8mM SB -16);

[0080] 6) Repeat steps 3), 4) and 5) in sequence once;

[0081] 7) Wash the human ulnar nerve with 0.01 MPBS solution for 35 minutes to obtain the product.

[0082] The allo...

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Abstract

The invention discloses a new kind of nerve allotransplant, the preparation method and its medical application. The invention employs chemical extraction method to remove the schwann cell, axon and myelin sheath that can cause rejection for nerve allotransplant, stores stroma cell and three-dimensional pipeline structure for axon regeneration, and provides intact regenerative frame. The physical and biochemical property of nerve allotransplant are similar to that of normal nerve, and can be used as nerve allotransplant for damaged nerve.

Description

technical field [0001] The present invention relates to a nerve graft material, its preparation method and its application, in particular to a kind of allogeneic nerve graft in which the Schwann cells, axons and myelin sheaths that cause rejection are completely removed, but at the same time the basement membrane tube remains intact and the clinical application of the allogeneic nerve graft belong to the field of medicine. Background technique [0002] The repair of peripheral nerve defects is a clinical problem, and its treatment should focus on solving two problems, one is the bridging repair of nerve defects, and the other is the selective docking of nerve fibers with distant target organs. At present, autologous nerve transplantation is still the standard operation for solving nerve defects in the clinical treatment process. However, due to the limited source of autologous nerves and many complications at the donor site, the medical community has been looking for biomate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36
Inventor 卢世璧孙明学王冠军李智彭江张莉赵斌王鑫许文静田玥
Owner GENERAL HOSPITAL OF PLA
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