The invention discloses a cavitation-bubble-mediated laser transfection method. According to the method, laser is used to irradiate nano particles with especially strong light absorptivity, the nano particles are combined with a specific cell through a specific ligand and a specific receptor, and cavitation bubbles are generated at the peripheries of the nano particles which are irradiated by the laser. The generation of the cavitation bubbles causes a cell membrane to be temporarily permeable, and thus the transfection of an allogenic material is achieved. The laser is contactless and free from pollution and cytotoxicity, so that the safe gene transfection method is realized; and moreover, as the cell membrane is caused to be permeable through generating the cavitation bubbles, the method is a physical method, can realize the transfection of any type of cells, can realize transfection in large area, and does not need special, expensive and precision instruments.