Cavitation-bubble-mediated laser cell transfection method

Abstract

The invention discloses a cavitation-bubble-mediated laser transfection method. According to the method, laser is used to irradiate nano particles with especially strong light absorptivity, the nano particles are combined with a specific cell through a specific ligand and a specific receptor, and cavitation bubbles are generated at the peripheries of the nano particles which are irradiated by the laser. The generation of the cavitation bubbles causes a cell membrane to be temporarily permeable, and thus the transfection of an allogenic material is achieved. The laser is contactless and free from pollution and cytotoxicity, so that the safe gene transfection method is realized; and moreover, as the cell membrane is caused to be permeable through generating the cavitation bubbles, the method is a physical method, can realize the transfection of any type of cells, can realize transfection in large area, and does not need special, expensive and precision instruments.

Description

technical field [0001] The invention belongs to the technical field of exogenous cell transfection, and relates to a cell laser transfection method mediated by cavitation bubbles. Background technique [0002] Genetic engineering technology is widely used in food, medicine and health, agriculture, industry, environmental protection and governance, and has brought certain social and economic benefits to human beings. In particular, gene therapy, which uses gene technology to introduce normal functional genes into target cells to repair defective tissues, has brought good news to human society. In the early 1990s, with the successful treatment of the world's first severe immunodeficiency disease with adenosine deaminase deficiency, gene therapy began to enter the clinical application stage, and its potential in the treatment of tumors, genetic diseases, and infectious diseases became more and more important. more and more people's attention. The gene transfection technology,...

AI Technical Summary

Problems solved by technology

However, since this method can only process one cell at a time, the efficiency is very low, and the cell death rate is high, so this method is not suitable for gene transfection of a large number of cells

On the other hand, during the operation of this method, precision instruments such as microscopes and optical tweezers are needed, and some methods also use very expensive femtosecond lasers

Therefore, this method has not been widely used so far.

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